BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,494,076	+C	11.8%	intergenic (+124/‑118)	RCAP_rcc02319 → / → RCAP_rcc02320	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,494,076	0	.→C	11.8%	36.4 ‑2.8 ‑1.2	117	intergenic (+124/‑118)	RCAP_rcc02319/RCAP_rcc02320	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (4/8): ref base (66/37): total (71/46)
Fisher's exact test strand distribution p-value = 5.88e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.81e-02

Only 100 of 133 total aligned reads displayed.


AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493977‑2494170


aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGCa                                                                                                  >  1:4364755/1‑101 (MQ=18)


 aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCag                                                                                                 <  2:413502/101‑2 (MQ=18)


 aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCag                                                                                                 <  1:2028967/101‑2 (MQ=18)


  gCGGACTCCCCCCGGGATTTTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCagc                                                                                                <  2:4145191/101‑3 (MQ=18)


  gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCagc                                                                                                >  2:450441/1‑99 (MQ=18)


    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGCagcgc                                                                                              >  1:815323/1‑97 (MQ=17)


       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cc                                                                                        >  2:688702/1‑101 (MQ=17)


        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.ccc                                                                                       >  1:2395499/1‑101 (MQ=17)


        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCG.cc.ccc                                                                                       <  2:4142574/101‑1 (MQ=17)


         ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccc                                                                                      >  1:2365263/1‑100 (MQ=17)


         ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccc                                                                                      <  2:1952332/101‑2 (MQ=17)


          cccccGGGATATTTGCAGCAAGATGAAACCGGATCCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccct                                                                                     <  1:19700/101‑3 (MQ=17)


           ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccctg                                                                                    <  1:4366253/101‑4 (MQ=17)


           ccccGGGATATGTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccctg                                                                                    <  2:1322038/101‑4 (MQ=17)


             ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccctgcc                                                                                  <  2:2951645/101‑6 (MQ=17)


              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGACC.CCCTGccc                                                                                  >  2:1214419/1‑101 (MQ=17)


               gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGcccc                                                                                 <  1:2186554/101‑1 (MQ=0)


               gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGcccc                                                                                 >  1:2784255/1‑101 (MQ=0)


               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGcccc                                                                                 <  1:3208022/101‑1 (MQ=17)


               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGcccc                                                                                 >  2:1325244/1‑101 (MQ=18)


               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGcccc                                                                                 >  1:576951/1‑101 (MQ=17)


                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcg                                                                                >  2:1447132/1‑101 (MQ=0)


                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.GGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcg                                                                                >  1:4009471/1‑101 (MQ=17)


                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgc                                                                               <  1:2793530/101‑2 (MQ=0)


                 gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcgg                                                                               >  1:2613171/1‑101 (MQ=17)


                  gtatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                                              <  2:1429237/100‑3 (MQ=2)


                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                                              <  1:1155047/101‑3 (MQ=2)


                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                                              <  2:2819530/101‑3 (MQ=2)


                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggcg                                                                             >  2:3118786/1‑101 (MQ=18)


                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.A.GCCAGAGGGGGC.AGCGCCC.CCCTGCCCcggcgg                                                                            >  2:225289/1‑101 (MQ=17)


                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCACcggcg                                                                             >  2:341801/1‑101 (MQ=17)


                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggcgg                                                                            >  1:947985/1‑101 (MQ=17)


                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGCAGCAC.CC.CCCTGCCCcggcgg                                                                            >  1:501559/1‑101 (MQ=17)


                    anNTGCNGCAAGATGAANGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccgg                                                                            <  2:1707762/101‑1 (MQ=2)


                      ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccggat                                                                          >  2:91130/1‑99 (MQ=2)


                      ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTc                                                                          <  2:3706895/101‑1 (MQ=17)


                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.CCGCCCTGCCCCGGCGGTcc                                                                         >  2:1376656/1‑101 (MQ=18)


                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCg                                                                        >  2:3410585/1‑101 (MQ=18)


                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGTGC.AGCGCCC.CCCTGCCCCGGCGGTCCGt                                                                       >  1:1490453/1‑101 (MQ=18)


                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCCGCGGGCCgg                                                                       >  2:3847046/1‑100 (MQ=14)


                          agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTg                                                                      >  2:1075039/1‑101 (MQ=18)


                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                                                                    >  1:3860326/1‑101 (MQ=18)


                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                                                                    <  1:3185265/101‑1 (MQ=17)


                             aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcg                                                                   <  2:1110206/101‑1 (MQ=17)


                             aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcg                                                                   >  2:2922315/1‑101 (MQ=18)


                                 tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGgcgc                                                               <  2:225445/101‑1 (MQ=17)


                                 tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGgcgc                                                               >  2:4269411/1‑101 (MQ=17)


                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCt                                                             <  1:4261496/101‑1 (MQ=17)


                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                                            <  1:1249351/101‑1 (MQ=17)


                                     aCCGGATGCCTTTCATCTTGCCCGAAATATCCTGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                                           >  2:3138641/1‑101 (MQ=18)


                                     aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                                           <  1:2335769/101‑1 (MQ=17)


                                      ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTgc                                                          >  1:3157711/1‑100 (MQ=17)


                                       cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGAGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                         >  2:3217971/1‑101 (MQ=18)


                                       cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.ACG.CCAGAGGGGGGC.CGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                         >  2:2470170/1‑101 (MQ=18)


                                       cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGGGGCGGCGCCTTGAc                                                         >  1:2467268/1‑101 (MQ=17)


                                       cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAGAGGGGGGC.CGCGCCC.CCCTGCCCCGGCGGCCCGGGGCGGGGCCTTgcc                                                         >  1:3069832/1‑99 (MQ=12)


                                       cGGATGCCTTTCATCTTGCCCGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                         <  1:1762593/101‑1 (MQ=17)


                                        ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGaag                                                        >  2:590309/1‑99 (MQ=17)


                                         gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGGGCCTTGAcgg                                                       >  1:1750029/1‑101 (MQ=17)


                                           tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCACCGC.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                                     <  1:2541303/101‑1 (MQ=18)


                                             ccTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                                   <  2:2365113/101‑1 (MQ=17)


                                             ccTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.GGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                                   <  1:4447110/101‑1 (MQ=17)


                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                 <  2:2395349/101‑1 (MQ=17)


                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                 >  1:33748/1‑101 (MQ=18)


                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                                               >  2:940965/1‑101 (MQ=18)


                                                  cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGGGCCGCCTTGAGGGGCGGGGgcg                                              >  2:1158134/1‑101 (MQ=17)


                                                  cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                                              >  2:1887942/1‑101 (MQ=18)


                                                   aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                                             >  1:4316494/1‑101 (MQ=18)


                                                        gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggggatcggaa                                        >  2:3740502/1‑93 (MQ=17)


                                                        gCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.A.GCCAGGGGGGGGCAGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                                        >  1:3528497/1‑101 (MQ=21)


                                                          ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                                      <  1:3502492/101‑1 (MQ=18)


                                                           ggAAGTATCCGGGGGGGTGAA.TGGC..GAA.G.CCGGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                                     <  1:1523195/100‑1 (MQ=21)


                                                           cGAAATAGCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                                     <  2:883167/101‑1 (MQ=18)


                                                                  tcggggggggTGAA.TGGC..G.AGG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa                              <  1:3249841/97‑1 (MQ=25)


                                                                    ccGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt                            >  2:1775408/1‑101 (MQ=21)


                                                                    ccGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt                            <  2:3162691/101‑1 (MQ=21)


                                                                     cGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAACGAAAttc                           >  2:683660/1‑100 (MQ=21)


                                                                       gggggTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                         >  2:1844584/1‑101 (MQ=25)


                                                                        ggggTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa                        >  1:2041783/1‑101 (MQ=25)


                                                                          ggTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa                      <  1:3944784/101‑1 (MQ=25)


                                                                          ggTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa                      <  1:688782/101‑1 (MQ=25)


                                                                           gTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg                     >  1:1934737/1‑101 (MQ=25)


                                                                           gTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg                     <  1:744363/101‑1 (MQ=25)


                                                                                       gcgaagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGAtgctgc              <  1:4198661/91‑1 (MQ=38)


                                                                                       gcgaagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGAtgctgc              <  1:151488/91‑1 (MQ=38)


                                                                                         gaagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            <  1:303296/93‑1 (MQ=38)


                                                                                         gaagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            >  2:1945454/9‑101 (MQ=38)


                                                                                          aagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAt           >  1:1249893/8‑101 (MQ=38)


                                                                                           agccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATc          <  2:2803826/95‑1 (MQ=38)


                                                                                           agccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATc          >  2:4443256/7‑101 (MQ=38)


                                                                                             ccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        <  1:1145100/97‑1 (MQ=38)


                                                                                             ccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        >  2:2189416/5‑101 (MQ=38)


                                                                                             ccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGAGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        <  2:3401636/97‑1 (MQ=38)


                                                                                              cagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc       >  1:2646775/4‑101 (MQ=38)


                                                                                              cagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc       >  2:152024/4‑101 (MQ=38)


                                                                                               agaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGTGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  1:615203/3‑101 (MQ=38)


                                                                                               agaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAGTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  1:3086947/3‑101 (MQ=38)


                                                                                                  ggggggC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGccc   >  2:3116917/1‑101 (MQ=255)


                                                                                                  ggggggC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGccc   >  1:687343/1‑101 (MQ=255)


                                                                                                   gggggC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCa  >  1:2624509/1‑101 (MQ=255)


AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493977‑2494170

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 23 ≤


ATCG

< 31 ≤


ATCG

< 40 ≤


ATCG