Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,080 +C 86.0% intergenic (+128/‑114) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0801.→C86.0% 43.8 ‑2.5 ‑0.2107intergenic (+128/‑114)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (60/31):  ref base (6/9):  total (67/40)
Fisher's exact test strand distribution p-value = 8.28e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.80e-01

Only 100 of 125 total aligned reads displayed.

CTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493984‑2494170
                                                                                                       |                                                                                           
 tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.ccc                                                                                       >  1:2395499/1‑101 (MQ=17)
 tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCG.cc.ccc                                                                                       <  2:4142574/101‑1 (MQ=17)
  ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccc                                                                                      >  1:2365263/1‑100 (MQ=17)
   cccccGGGATATTTGCAGCAAGATGAAACCGGATCCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccct                                                                                     <  1:19700/101‑3 (MQ=17)
    ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAT.TGGC..GAA.G.CCGGGGGGGGGCGGGCC.cc.ccccc                                                                                     >  2:3136668/1‑99 (MQ=17)
    ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccctg                                                                                    <  1:4366253/101‑4 (MQ=17)
    ccccGGGATATGTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccctg                                                                                    <  2:1322038/101‑4 (MQ=17)
      ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccctgcc                                                                                  <  2:2951645/101‑6 (MQ=17)
       cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGACC.CCCTGccc                                                                                  >  2:1214419/1‑101 (MQ=17)
       cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATACCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGccc                                                                                  >  1:2563268/1‑101 (MQ=17)
        gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGcccc                                                                                 >  1:2784255/1‑101 (MQ=0)
        gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGcccc                                                                                 <  1:2186554/101‑1 (MQ=0)
        gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGcccc                                                                                 <  1:3208022/101‑1 (MQ=17)
        gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGcccc                                                                                 >  1:576951/1‑101 (MQ=17)
        gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGcccc                                                                                 >  2:1325244/1‑101 (MQ=18)
         ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcg                                                                                >  2:1447132/1‑101 (MQ=0)
         ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.GGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcg                                                                                >  1:4009471/1‑101 (MQ=17)
         ggATANNTGCAGAAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcg                                                                                <  1:1594892/101‑1 (MQ=17)
          gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgc                                                                               >  2:1683206/1‑100 (MQ=2)
           atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                                              <  1:1155047/101‑3 (MQ=2)
            tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.A.GCCAGAGGGGGC.AGCGCCC.CCCTGCCCcggcgg                                                                            >  2:225289/1‑101 (MQ=17)
            tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCACcggcg                                                                             >  2:341801/1‑101 (MQ=17)
             atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggcgg                                                                            >  1:947985/1‑101 (MQ=17)
             atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGCAGCAC.CC.CCCTGCCCcggcgg                                                                            >  1:501559/1‑101 (MQ=17)
             anNTGCNGCAAGATGAANGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccgg                                                                            <  2:1707762/101‑1 (MQ=2)
               ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccggat                                                                          >  2:91130/1‑99 (MQ=2)
                tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.CCGCCCTGCCCCGGCGGTcc                                                                         >  2:1376656/1‑101 (MQ=18)
                 gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCg                                                                        >  2:3410585/1‑101 (MQ=18)
                  cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGTGC.AGCGCCC.CCCTGCCCCGGCGGTCCGt                                                                       >  1:1490453/1‑101 (MQ=18)
                  cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCCGCGGGCCgg                                                                       >  2:3847046/1‑100 (MQ=14)
                   agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTg                                                                      >  1:3046297/1‑101 (MQ=17)
                    gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTgg                                                                     >  1:1966347/1‑101 (MQ=17)
                     caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                                                                    <  1:3185265/101‑1 (MQ=17)
                     caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                                                                    >  2:503747/1‑101 (MQ=17)
                      aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcg                                                                   >  2:2922315/1‑101 (MQ=18)
                      aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcg                                                                   <  2:1110206/101‑1 (MQ=17)
                        gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAAAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcggc                                                                 <  1:2171140/101‑1 (MQ=18)
                          tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGgcgc                                                               <  2:225445/101‑1 (MQ=17)
                          tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.CCTCCCTGCCCCGGCGGTCCGTGGCGgggc                                                               >  1:414463/1‑101 (MQ=17)
                             aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                                            <  1:1249351/101‑1 (MQ=17)
                              aCCGGATGCCTTTCATCTTGCCCGAAATATCCTGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                                           >  2:3138641/1‑101 (MQ=18)
                              aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                                           <  1:2335769/101‑1 (MQ=17)
                                cGGATTCCTTTCATCTTGCCGGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                         <  2:3625771/101‑1 (MQ=14)
                                cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                         >  2:1859906/1‑101 (MQ=18)
                                cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGAGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                         >  2:3217971/1‑101 (MQ=18)
                                cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.ACG.CCAGAGGGGGGC.CGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                         >  2:2470170/1‑101 (MQ=18)
                                cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGGGGCGGCGCCTTGAc                                                         >  1:2467268/1‑101 (MQ=17)
                                cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAGAGGGGGGC.CGCGCCC.CCCTGCCCCGGCGGCCCGGGGCGGGGCCTTgcc                                                         >  1:3069832/1‑99 (MQ=12)
                                cGGATGCCTTTCATCTTGCCCGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                         <  1:1762593/101‑1 (MQ=17)
                                 ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGaag                                                        >  2:590309/1‑99 (MQ=17)
                                    tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCACCGC.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                                     <  1:2541303/101‑1 (MQ=18)
                                      ccTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.GGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                                   <  1:4447110/101‑1 (MQ=17)
                                        tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                 <  2:2395349/101‑1 (MQ=17)
                                        tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                 >  1:33748/1‑101 (MQ=18)
                                         ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                                                >  2:109218/1‑101 (MQ=18)
                                          tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                                               >  2:940965/1‑101 (MQ=18)
                                           cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGGGCCGCCTTGAGGGGCGGGGgcg                                              >  2:1158134/1‑101 (MQ=17)
                                           cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                                              >  2:1887942/1‑101 (MQ=18)
                                            aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                                             >  1:4316494/1‑101 (MQ=18)
                                            aTCTTGCCCGAAATATCCCGGGGGGTGAA.TAGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                                             <  1:621085/101‑1 (MQ=18)
                                              cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAGGGGCGGGGGGGCgg                                           >  1:2783674/1‑101 (MQ=17)
                                              cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                                           <  2:1427906/101‑1 (MQ=18)
                                                 gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggggatcggaa                                        >  2:3740502/1‑93 (MQ=17)
                                                 gCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.A.GCCAGGGGGGGGCAGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                                        >  1:3528497/1‑101 (MQ=21)
                                                   ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                                      <  1:3502492/101‑1 (MQ=18)
                                                   ccGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                                      >  2:2103695/1‑101 (MQ=18)
                                                    ggAAGTATCCGGGGGGGTGAA.TGGC..GAA.G.CCGGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                                     <  1:1523195/100‑1 (MQ=21)
                                                    cGAAATAGCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                                     <  2:883167/101‑1 (MQ=18)
                                                           tcggggggggTGAA.TGGC..G.AGG.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa                              <  1:3249841/97‑1 (MQ=25)
                                                             ccGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt                            <  2:3162691/101‑1 (MQ=21)
                                                              cGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAACGAAAttc                           >  2:683660/1‑100 (MQ=21)
                                                               ggggggTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGGGGGGGCGCGGGGATTAACCGAAAATGc                          >  1:810231/1‑101 (MQ=18)
                                                                gggggTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                         >  2:1844584/1‑101 (MQ=25)
                                                                gggggTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                         >  2:762742/1‑101 (MQ=25)
                                                                   ggTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa                      <  1:3944784/101‑1 (MQ=25)
                                                                   ggTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa                      <  1:688782/101‑1 (MQ=25)
                                                                    gTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg                     >  1:1934737/1‑101 (MQ=25)
                                                                    gTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg                     <  1:744363/101‑1 (MQ=25)
                                                                     tGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa                    <  2:2936647/101‑1 (MQ=25)
                                                                     tGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa                    >  1:4331794/1‑101 (MQ=25)
                                                                                gcgaagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGAtgctgc              <  1:4198661/91‑1 (MQ=38)
                                                                                gcgaagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGAtgctgc              <  1:151488/91‑1 (MQ=38)
                                                                                  gaagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGGGa            >  2:2567155/9‑101 (MQ=37)
                                                                                  gaagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            <  1:303296/93‑1 (MQ=38)
                                                                                  gaagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            >  2:1945454/9‑101 (MQ=38)
                                                                                   aagccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAt           >  1:1249893/8‑101 (MQ=38)
                                                                                    agccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATc          <  2:2803826/95‑1 (MQ=38)
                                                                                    agccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATc          >  2:4443256/7‑101 (MQ=38)
                                                                                      ccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        <  1:1145100/97‑1 (MQ=38)
                                                                                      ccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        >  2:2189416/5‑101 (MQ=38)
                                                                                      ccagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGAGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCt        <  2:3401636/97‑1 (MQ=38)
                                                                                       cagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc       >  1:2646775/4‑101 (MQ=38)
                                                                                       cagaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc       >  2:152024/4‑101 (MQ=38)
                                                                                        agaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGTGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  1:615203/3‑101 (MQ=38)
                                                                                        agaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAGTAACCGAAATTGCAACAGATGCTGCGATCCTCg      >  1:3086947/3‑101 (MQ=38)
                                                                                         gaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGGGATCCTCGc     >  1:3165267/2‑101 (MQ=38)
                                                                                         gaGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGc     <  2:4207857/100‑1 (MQ=39)
                                                                                           ggggggC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGccc   >  2:3116917/1‑101 (MQ=255)
                                                                                            gggggC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCa  >  1:3733740/1‑101 (MQ=255)
                                                                                            gggggC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCa  >  1:2624509/1‑101 (MQ=255)
                                                                                                       |                                                                                           
CTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493984‑2494170

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG