Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,160 +C 100% intergenic (‑136/+140) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1601.→C100.0% 300.6 99intergenic (‑136/+140)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (34/64):  ref base (1/0):  total (35/64)

Only 100 of 115 total aligned reads displayed.

CCGGTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAC  >  NC_014034/2640060‑2640255
                                                                                                      |                                                                                               
ccGGTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTgg                                                                                                  >  1:2959004/1‑101 (MQ=255)
   gTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.cct                                                                                              <  1:1095564/101‑3 (MQ=255)
   gTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.cct                                                                                              <  2:3313992/101‑3 (MQ=255)
   gTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.cct                                                                                              <  2:3159354/101‑3 (MQ=255)
   gTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.cct                                                                                              <  2:2848952/101‑3 (MQ=255)
    gtGCGGGTTATTATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.cctt                                                                                             <  2:1303434/100‑4 (MQ=255)
     tGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.ccttc                                                                                            <  2:1414767/101‑5 (MQ=255)
      gCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.ccttcg                                                                                           <  2:2235791/101‑6 (MQ=255)
      gCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.ccttcg                                                                                           <  1:509420/101‑6 (MQ=255)
       cGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCgg                                                                                           <  2:2825987/101‑1 (MQ=255)
        gggTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGc                                                                                          <  2:824401/101‑1 (MQ=255)
        gggTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGc                                                                                          <  2:519943/101‑1 (MQ=255)
          gTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCa                                                                                        <  2:3596109/101‑1 (MQ=255)
           ttATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCaa                                                                                       <  2:1560061/101‑1 (MQ=255)
           tgatgatgCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGACaa                                                                                       <  1:803219/99‑1 (MQ=255)
            tATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAAt                                                                                      <  2:2825399/101‑1 (MQ=255)
             atgatgCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAAtt                                                                                     <  1:2686796/101‑1 (MQ=255)
              tgatgCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAGCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTc                                                                                    <  1:1671355/101‑1 (MQ=255)
              tgatgCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTc                                                                                    <  2:4045769/101‑1 (MQ=255)
               gatgCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTca                                                                                   <  1:3866323/101‑2 (MQ=255)
                atgCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTcac                                                                                  <  2:2337753/101‑3 (MQ=255)
                  gcgcCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCAccc                                                                                <  1:575799/101‑1 (MQ=255)
                    gcggggTATCGGACGGGGCGGCAGGGGTGTCACCGGTGCCGCTTTTGCGCAGGCAAAGGGGGGGCGCTCCCCCCCTC.TTGGCCTTCGGCAAATTCCccccc                                                                              <  2:2554570/98‑1 (MQ=16)
                      cGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCg                                                                            <  2:260983/101‑1 (MQ=255)
                        ggTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAg                                                                          <  2:3215552/101‑1 (MQ=255)
                        ggTATGCGACGCGGCGGCAGGCGTGTCAACCGTCGCGCTTTTGCGCAGGCAAAAGGGCGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAg                                                                          <  1:3809351/101‑1 (MQ=37)
                              ggACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTAttt                                                                    <  1:2306650/100‑1 (MQ=255)
                              cGACGGGGCTGTAGTCGTGTCAACCGTGGCGCTTTTGCCCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTAttt                                                                    <  2:3937291/101‑1 (MQ=21)
                              cGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTAttt                                                                    <  1:4444468/101‑1 (MQ=255)
                                aCGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTgg                                                                  <  2:497954/101‑1 (MQ=255)
                                  gcggcgGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGag                                                                <  2:3005194/101‑2 (MQ=255)
                                   cggcggCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGagc                                                               <  2:4286988/101‑3 (MQ=255)
                                       ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                           <  2:70213/101‑1 (MQ=37)
                                       ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTAACCCCCCGAGGTATTTGGAGCAAGa                                                           >  2:1463435/1‑101 (MQ=25)
                                         gaGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                         <  2:2587362/100‑1 (MQ=25)
                                         cAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                         <  1:146990/101‑1 (MQ=37)
                                            gcggtgccaCCGGGGCCGTTTTGCGCCAGCAAAAGGGGGGCCCCGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                      <  1:2615763/93‑1 (MQ=12)
                                              gtgtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                    <  2:2107149/101‑3 (MQ=25)
                                                   aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                              <  1:94532/101‑1 (MQ=25)
                                                     ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                            <  2:3936702/101‑1 (MQ=25)
                                                         ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                        <  1:3877557/101‑1 (MQ=25)
                                                            gcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCCTCGGCCAATTCACCCCCCCGGGGATTTGGAGGAAGATG.AAAGAACAAGGGTTCATcc                                     >  1:1390025/1‑100 (MQ=16)
                                                              ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTTGC.CTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATGAAAA.ACCATGGGTTCATCTTg                                   >  2:4264698/1‑101 (MQ=21)
                                                              ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                   <  2:3070734/101‑1 (MQ=21)
                                                              ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                   <  2:3821418/101‑1 (MQ=25)
                                                                  gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                               <  2:2245420/101‑1 (MQ=25)
                                                                     gccccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                           >  2:381633/7‑101 (MQ=14)
                                                                      ccccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAata                          <  2:1672072/96‑1 (MQ=17)
                                                                       cccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                         <  1:1435728/97‑1 (MQ=17)
                                                                        ccgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATc                        >  2:3812970/4‑101 (MQ=17)
                                                                         cgaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                       >  1:2516379/3‑101 (MQ=17)
                                                                         cAAAAGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATc                        <  1:1662996/101‑1 (MQ=25)
                                                                          gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                      <  2:1214460/100‑1 (MQ=17)
                                                                          gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                      >  1:983552/2‑101 (MQ=17)
                                                                          gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                      <  2:2584643/100‑1 (MQ=17)
                                                                          gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                      >  2:2237596/2‑101 (MQ=17)
                                                                          gaaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                      >  1:3221725/2‑101 (MQ=17)
                                                                          aaaaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATcc                       <  1:1864880/101‑1 (MQ=21)
                                                                           aaaGGGGGGCGCTGCCGCCCTC.TTGGCCTTCGGTCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                      <  2:2034580/101‑1 (MQ=25)
                                                                           aaaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCCATTCACCCCCCGAGGGATTTGGGGCAAGATG.AAAGACCATAGGGTCATCTTGCCTCAAATATccc                      >  2:4382118/1‑101 (MQ=18)
                                                                           aaaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACGCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                      <  2:2958783/101‑1 (MQ=25)
                                                                           aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                     >  1:501348/1‑101 (MQ=17)
                                                                           aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                     >  1:3317379/1‑101 (MQ=17)
                                                                           aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                     >  1:284981/1‑101 (MQ=17)
                                                                           aaaAGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                     <  2:4434161/101‑1 (MQ=17)
                                                                            aaagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                    >  2:1754790/4‑101 (MQ=17)
                                                                            aaagggagCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                    <  1:151104/98‑1 (MQ=18)
                                                                            aaGGGGGGCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                     <  2:3915414/101‑1 (MQ=25)
                                                                             aagggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggg                   >  2:3995351/3‑101 (MQ=18)
                                                                              ggggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggg                   <  1:3064119/101‑1 (MQ=25)
                                                                               gggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                 >  1:2364239/1‑101 (MQ=18)
                                                                               gggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCggggg                 <  2:2442551/101‑1 (MQ=18)
                                                                                ggggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                <  2:4170680/101‑2 (MQ=18)
                                                                                 gggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg               >  1:1483656/1‑99 (MQ=18)
                                                                                 gggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                <  1:3798836/101‑1 (MQ=25)
                                                                                 gggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGGTCATCTTGCCTCAAATATCCCgggggg                >  1:2066226/1‑101 (MQ=21)
                                                                                  ggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga              <  2:2365971/101‑4 (MQ=21)
                                                                                  ggCGCTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa               <  1:1569019/101‑1 (MQ=25)
                                                                                   gcgcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag             <  1:371556/101‑5 (MQ=21)
                                                                                   gcgcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag             <  2:1301882/101‑5 (MQ=21)
                                                                                   gcgcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGACATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag             >  2:170973/1‑97 (MQ=21)
                                                                                   gcgcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTTTTTGGAGCAAGATG.AAAGAACATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAg              >  1:1491290/1‑101 (MQ=25)
                                                                                    cgcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg            <  2:3386072/101‑6 (MQ=21)
                                                                                     gcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc           >  2:2897688/1‑95 (MQ=21)
                                                                                     gcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGCGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggtgaggc           >  1:2673094/1‑95 (MQ=21)
                                                                                     gcTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGGGTc            >  1:2493273/1‑101 (MQ=25)
                                                                                      gctgcccccTC.TTGGCCTTTGGCCAATTC.CCCCCTGGGATATTTCGGGCAAGATGAAATCA.TGCCCCTTCATCTTGCCGCAAATATCCCGGGGGGAgcgaa          <  2:519174/97‑5 (MQ=2)
                                                                                      cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcg          >  1:157205/1‑94 (MQ=25)
                                                                                      cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc           >  2:258998/1‑101 (MQ=25)
                                                                                      cTGCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc           >  2:1747285/1‑101 (MQ=25)
                                                                                        gCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggggaggcgaa        >  2:60573/1‑93 (MQ=21)
                                                                                        gCCCCCCTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg         <  1:1819846/101‑1 (MQ=37)
                                                                                          tctccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa       <  2:3142271/98‑1 (MQ=37)
                                                                                          cccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa       <  2:1107672/101‑1 (MQ=37)
                                                                                          cccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa       >  1:3381854/1‑101 (MQ=37)
                                                                                           ccccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa      >  2:331491/1‑101 (MQ=37)
                                                                                             ccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg    >  2:1598174/1‑101 (MQ=37)
                                                                                             ccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg    <  1:2747996/101‑1 (MQ=37)
                                                                                             ccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg    >  2:192453/1‑101 (MQ=37)
                                                                                             ccTC.TTGGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAga    >  1:3142495/1‑100 (MQ=37)
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CCGGTTGCGGGTTATGATGCGCCGGGTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTC.TTGG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAC  >  NC_014034/2640060‑2640255

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG