Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,678,900 +C 100% intergenic (+29/+58) RCAP_rcc02495 → / ← RCAP_rcc02496 GTPase, EngC family/group 1 glycosyl transferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,678,9001.→C100.0% 19.5 110intergenic (+29/+58)RCAP_rcc02495/RCAP_rcc02496GTPase, EngC family/group 1 glycosyl transferase
Reads supporting (aligned to +/- strand):  new base (64/37):  ref base (0/8):  total (65/45)
Fisher's exact test strand distribution p-value = 5.67e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.11e-01

Only 100 of 142 total aligned reads displayed.

GCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CG.CCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGCG  >  NC_014034/2678800‑2678993
                                                                                                        |                                                                                               
gCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.Cg                                                                                                  <  1:3475967/101‑1 (MQ=255)
  ccGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CG.cc                                                                                               <  1:2151099/101‑1 (MQ=255)
   cgcgACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CG.ccc                                                                                              >  1:1243792/1‑101 (MQ=255)
   cgcgACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CG.ccc                                                                                              <  1:2781706/101‑1 (MQ=255)
   cgcgACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGCGGGG.GCA.T.CG.ccc                                                                                              >  2:2618352/1‑101 (MQ=255)
      ggccGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAAGCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CG.ccccca                                                                                           <  2:646659/99‑3 (MQ=255)
      gACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CG.cccccc                                                                                           >  2:3083563/1‑99 (MQ=255)
      gACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CG.ccccca                                                                                           >  2:4419174/1‑99 (MQ=255)
      gACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CG.ccccca                                                                                           >  2:1412429/1‑99 (MQ=255)
        ccGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CG.cccccacc                                                                                         <  2:2740916/101‑5 (MQ=255)
         cgcgCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CG.cccccaccg                                                                                        >  1:3033310/1‑96 (MQ=255)
         cgcgCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CG.cccccaccg                                                                                        <  1:2864171/101‑6 (MQ=255)
          gcgcCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.Cc.gc                                                                                      >  2:1062684/1‑101 (MQ=255)
           cgcCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.Cc.gcg                                                                                     <  2:3918874/101‑1 (MQ=255)
           cgcCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.Cc.gcg                                                                                     <  2:2379495/101‑1 (MQ=255)
             ccTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGcc                                                                                   <  2:2100443/101‑1 (MQ=255)
             ccTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGcc                                                                                   >  2:4175443/1‑101 (MQ=255)
              ctgcGGAAAGATGCCCGGGGCGGCGCTGCGCGACAAACCCCGGCGGCGCGGCGACTGACGCCGTTTCGGGGGCCGGTGGGG.GCA.T.CCCCCCCA.CC.GCGccc                                                                                  <  2:3975913/98‑1 (MQ=255)
              cTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GGA.T.CGCCCCCC.CC.GCGccc                                                                                  >  2:3308641/1‑101 (MQ=255)
              cTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTC.GGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCg                                                                                 >  1:188412/1‑101 (MQ=255)
               ttCGGCAAGATGCCCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCg                                                                                 <  2:296479/101‑1 (MQ=255)
                tCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCgc                                                                                >  1:2952829/1‑100 (MQ=255)
                  ggCAAGATGCCCCGGGCGGCGCGCCGCGACAAACCCGGGCTGCGCGGCGACTGACGCCGTTTGGGGGGCGGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGAcg                                                                              <  2:3740780/101‑1 (MQ=255)
                  ggCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGAcg                                                                              >  2:1044296/1‑101 (MQ=255)
                    cAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGAcgcg                                                                            >  1:4144419/1‑101 (MQ=255)
                     aaGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGAcgcgc                                                                           >  2:3289362/1‑101 (MQ=255)
                     aaGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGAcgcgc                                                                           <  1:4237464/101‑1 (MQ=255)
                     aaGACGCCCCGGGCGGCGCGCCGCAACAAACCCCGGCTGCGCGGGGACTGCCGCCGGTGGGGGGGCGGGGGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGAcgcgc                                                                           <  2:2330145/101‑1 (MQ=255)
                       gATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCgcgg                                                                         >  1:1161919/1‑101 (MQ=255)
                        aTGCCCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTGGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCgcggc                                                                        <  1:3542994/101‑1 (MQ=255)
                           aCCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCggg                                                                     <  2:4381238/101‑1 (MQ=255)
                           aCCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCggg                                                                     >  2:883573/1‑101 (MQ=255)
                              cGGGGGGGGGTGCGCGAAAACCCCCGGCTGGGGGGCGCGTGACGCGGGTGGGGGGGCGGGGGGGG.GCACC.CC.CCCCC.CC.GCGCCCGACGCGCGGCGGGCtt                                                                  <  1:4175704/101‑1 (MQ=9)
                              cGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCtt                                                                  >  2:3723897/1‑101 (MQ=255)
                               gggGGGCGCTGCGCGACAAACCCCGGCTGCGCGGCGACTGACGCCGGTGGGGGGGCGGGGGGGG.GCA.TCCC.CCCCA.CC.GCGCCCGACGCGCGGCGGGCTTg                                                                 <  1:3363637/101‑1 (MQ=25)
                                 gcggcgCGGCGCGACAAACCCCGGCTGGGCGGCGCCTGCCGCCGGTTCGGGGGCGGGGGGGG.GCA.TCCC.CCCCA.CC.GCGCCCGACGCGCGGCGGGCTTgcc                                                               <  1:1036839/101‑1 (MQ=25)
                                  gggcgCTGCGCGCAAACTCCCGGCGGGGGGGCGACTGACGCCGGTTGGGGGGCGGGGGGGG.GCA.TCCC.CCCCA.CC.GCGCCCGACGCGCGGCGGGCTTgccc                                                              <  1:8498/100‑1 (MQ=21)
                                   ggcgCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCtttcacc                                                             >  1:2491952/1‑96 (MQ=255)
                                   ggcgCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTgcccg                                                             >  1:207208/1‑101 (MQ=255)
                                     cgcTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTgcccccc                                                           >  2:1632125/1‑101 (MQ=255)
                                      gcTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTgcccgccc                                                          >  1:3991560/1‑101 (MQ=255)
                                      gcTGCGCGACAAACCCCGGCTGCGCGGCGACTGGCGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTgcccgccc                                                          <  2:1219416/101‑1 (MQ=38)
                                       cTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCCTGCCCGCCCa                                                         >  2:2243244/1‑101 (MQ=255)
                                       cTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGGGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCa                                                         >  1:3945572/1‑101 (MQ=255)
                                         gcgcGACAACTCCCGGCGGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag                                                       <  2:1651678/101‑1 (MQ=37)
                                            cgACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag                                                    >  1:2669675/1‑101 (MQ=255)
                                               caacgcccGGCGGCGCGGCGACGGCCGCCGGTTCGGGGGCCGGTGGGG.GCA.TCCC.CCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgc                                                 <  1:1812510/96‑1 (MQ=21)
                                               cAAGTCCGGGCGGCGGGGCGGGGGGCGCGGGTCGGGGGGCGGGGGGGG.GCACC.CC.CCCCG.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgc                                                 <  1:1676270/101‑1 (MQ=2)
                                               cAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgc                                                 <  1:1080953/101‑1 (MQ=255)
                                                aaaTCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcg                                                <  2:4051866/101‑1 (MQ=255)
                                                aaaTCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcg                                                >  2:3302730/1‑101 (MQ=255)
                                                aaaTCCCGGCTGCGCGGCGACTGACGCCGGTGCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcg                                                <  1:3995523/101‑1 (MQ=255)
                                                    cccGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGcccc                                            >  2:2719852/1‑101 (MQ=255)
                                                      cGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCg                                          <  2:509078/101‑1 (MQ=255)
                                                       ggCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCTCGGCGGGCTTGCCCGCCCAAGGGGGGGGCCCCCgg                                         >  1:1604858/1‑101 (MQ=37)
                                                       ggCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCgg                                         >  1:2672605/1‑101 (MQ=255)
                                                       ggCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGACCCCgg                                         >  1:3899851/1‑101 (MQ=255)
                                                        ggtggccggcgGCTGCCGCGGGTTGGGGGGCGGGGGGGGCCCC.C.CC.CCCCC.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGATGGCGCCCCCGGa                                        <  1:3191330/95‑1 (MQ=9)
                                                        gCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGa                                        >  1:3526311/1‑101 (MQ=255)
                                                          tGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAtc                                      >  2:363940/1‑101 (MQ=255)
                                                          tGCGCGGCGACTGACACCGGTTCGGGGGCCGGGGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGCGGGCGGCCCCGGAtc                                      >  2:380876/1‑101 (MQ=25)
                                                          ggcgcGGCGACTGCCGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAtc                                      <  2:1161828/100‑1 (MQ=255)
                                                           gcgcGGCGGCTGACGCGGGGCCGGGGGCGGGGGGGG.GCACC.CC.CCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAtct                                     <  2:1536036/101‑1 (MQ=18)
                                                           gcgcGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAtct                                     <  2:4166797/101‑1 (MQ=255)
                                                            cggggcgACTGCCGCCGTTGGGGGGCCCGGGGGGG.GCACC.CC.CCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAtctc                                    <  2:3097692/98‑1 (MQ=18)
                                                            cgcgGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAtctc                                    >  1:1547778/1‑101 (MQ=255)
                                                             gcggcgACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCa                                   <  1:3349680/101‑1 (MQ=255)
                                                               ggcgACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCCAGGAGGGCGCCCCCCGGTCTCAgc                                 >  2:2259869/1‑101 (MQ=25)
                                                               ggcgACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAgc                                 <  1:1412536/101‑1 (MQ=255)
                                                               ggcgACTGACGCCGGTTCGGGCGCCGGTGGGG.GCA.T.CC.CCCCACCC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAgc                                 <  2:1764106/101‑1 (MQ=37)
                                                                 cgACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAgcgc                               <  1:4419446/101‑1 (MQ=255)
                                                                  gACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAgcccc                              >  2:431297/1‑98 (MQ=255)
                                                                  gACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCa                              <  2:2944859/101‑1 (MQ=255)
                                                                    cTGACCCCGCCTCGGGCGCCGGTGGTG.GCA.T.CG.CCCCC.CCTGCTCCCGACGCGCGGCGGGCGTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAAc                            <  2:2048453/101‑1 (MQ=21)
                                                                       gcGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTg                         <  1:1062772/100‑1 (MQ=255)
                                                                       aCGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTg                         <  1:2143905/101‑1 (MQ=255)
                                                                       aCGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTg                         >  2:471645/1‑101 (MQ=255)
                                                                        cGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCCGCGCAACGTgc                        >  2:1225687/1‑101 (MQ=255)
                                                                           cGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTgccgc                     >  2:3660208/1‑101 (MQ=255)
                                                                           cGGTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACCTgccgc                     >  2:1143917/1‑101 (MQ=255)
                                                                            ggTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTgccgcc                    >  2:857735/1‑101 (MQ=255)
                                                                            ggTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTgccgcc                    <  1:4196954/101‑1 (MQ=255)
                                                                            ggTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTgccgcc                    >  2:228300/1‑101 (MQ=255)
                                                                            ggTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTgccgcc                    >  2:2759295/1‑101 (MQ=255)
                                                                             gTTCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGccgccc                   >  1:4290695/1‑101 (MQ=255)
                                                                              ttCGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGccgcccg                  <  2:2686016/101‑1 (MQ=255)
                                                                                cGGGGGCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCcgcccgcc                <  1:622479/101‑1 (MQ=255)
                                                                                 gggggCGGGGGGGG.GCC.C.CCCCCCCC.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCccgccg               <  1:1632231/101‑1 (MQ=21)
                                                                                 gggggCCGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCccgccg               <  2:1817639/101‑1 (MQ=255)
                                                                                  ggggCCGGTGGGG.CCA.C.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGt              <  2:759030/101‑1 (MQ=25)
                                                                                      ccGGTGGGG.GCA.TCCC.CCCCC.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCg          <  1:981939/101‑1 (MQ=25)
                                                                                       cGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGa         >  1:249406/1‑101 (MQ=255)
                                                                                       cGGTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGCGCCGCCCGCCGTCTCGa         >  1:848431/1‑101 (MQ=255)
                                                                                        ggTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGAc        <  2:3613324/101‑1 (MQ=255)
                                                                                        ggTGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGAc        >  2:1438199/1‑101 (MQ=255)
                                                                                          tGGGG.GCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACaa      >  2:1337899/1‑101 (MQ=255)
                                                                                           gatg.gCA.T.CGTCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGCCTCggaaaa     >  2:3418043/4‑96 (MQ=25)
                                                                                            ggg.gCA.C.CGCCCCCC.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAg    <  2:3991304/101‑1 (MQ=25)
                                                                                              g.gCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGCg  >  2:4019956/1‑101 (MQ=255)
                                                                                               agCA.T.CGCCCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGCg  >  1:3904138/2‑101 (MQ=255)
                                                                                                        |                                                                                               
GCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGG.GCA.T.CG.CCCCA.CC.GCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGCG  >  NC_014034/2678800‑2678993

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG