Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,849	+G	100%	intergenic (‑181/+112)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,849	1	.→G	100.0%	58.6	19	intergenic (‑181/+112)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (2/17):  ref base (0/0):  total (2/17)

GTTCGGGCGGAATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GG..CCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGG  >  NC_014034/2747749‑2747943                                                                                                      |                                                                                                  gtgcGGGGGAAATAGAAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc                                                                                                    <  2:3519727/98‑1 (MQ=255) ggtggggCGGAATAGGAACCGCGCCCTGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc                                                                                                    <  2:2444205/97‑1 (MQ=255) gTTCGGGCGGAATAGGAACCGCGCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGAGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc                                                                                                    <  1:1276197/101‑1 (MQ=255)  ttCGGGCGGAATAGAAAACGCGGCCCGCGTCTCCCCGCCCGCAGCGCCCGGTTGCGGCATGCCCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.g                                                                                                  <  2:754216/101‑1 (MQ=255)   tggggCGGAAAAGGAAACGCGCCCCGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.gg                                                                                                 <  1:3774888/99‑2 (MQ=255)    ggggCGGAAGAGGAAACGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggc                                                                                                <  1:3982800/100‑3 (MQ=39)    cGGGCGGAATAGGAAACGCGCCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggc                                                                                                <  2:310373/101‑3 (MQ=255)     gggCGGAATAGGAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcg                                                                                               <  2:4100345/101‑4 (MQ=39)       ggggAATAGGAAACCCGCCCCGCGTTCCCCCACCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcggg                                                                                             <  1:3205711/99‑6 (MQ=37)       gCGGAATGGAAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcggg                                                                                             <  2:3190201/101‑6 (MQ=37)       gCGGAATAGGAAACGCGNCCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcggg                                                                                             <  2:512722/101‑6 (MQ=255)       gCGGAATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcggg                                                                                             >  1:1032849/1‑96 (MQ=255)        cGGAATAGGAAACGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggccc.ggcgggc                                                                                            <  1:4230145/101‑7 (MQ=38)             tAGAAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGaa                                                                                        <  2:1032761/101‑1 (MQ=25)             aaGGAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGaa                                                                                        <  2:3037013/100‑1 (MQ=25)              aGGAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAg                                                                                       <  1:2444692/101‑1 (MQ=25)               ggaacggccccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                      <  1:545456/91‑1 (MQ=25)               ggAAACGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                      <  1:955445/101‑1 (MQ=37)               ggAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgc                                                                                      >  2:4194194/1‑101 (MQ=255)                gAACCGCGCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgcg                                                                                     <  1:1223420/101‑1 (MQ=25)                 aaccgcgCCCCGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAgcgc                                                                                    <  2:1698072/98‑1 (MQ=25)                   ccgcaccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgccc                                                                                  <  1:3181821/95‑1 (MQ=25)                     cccccccGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcccgc                                                                                <  1:2102195/97‑1 (MQ=21)                      cgccccGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcccgcc                                                                               <  2:1802792/98‑1 (MQ=21)                        ccccGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                            <  1:747995/100‑1 (MQ=255)                        ccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                            <  2:188383/100‑1 (MQ=25)                          ccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                          <  2:2395303/101‑3 (MQ=25)                          ccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                          <  1:702708/101‑3 (MQ=25)                           cgctTTCCCCCGCCCGCGGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                         <  2:4142621/101‑4 (MQ=21)                           cgctTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                         <  2:4133233/101‑4 (MQ=21)                               tccccccGCCCGCAGCGCCCGGTTGCGGCAGGACCGCTTCAGCCCGCACCACAGGGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                     <  1:3237609/98‑8 (MQ=21)                                ccccccGCCCGCAGCGCCCGGTTGCGGCAGGACCGCTTCACCCCGCCCCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgggcg                                                                    <  1:4116955/99‑9 (MQ=18)                                                                                            cgcCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGc       >  2:4142135/1‑101 (MQ=18)                                                                                             gcccggcccgGCGGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg      >  1:490430/1‑101 (MQ=18)                                                                                               ccggcccgGCGGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGAt    >  1:1645388/1‑101 (MQ=18)                                                                                                 ggcccgGCGGGCGAGAAGCGCCCGCC.GGGACCGGC.GCTGCCCGAGGGCCTTTGGGCCCTCGGGGGGCGCAGGCCCTCTGTGACGCATGGCCAAGGCGATgg  >  1:3661614/1‑101 (MQ=18)                                                                                                      |                                                                                                  GTTCGGGCGGAATAGGAAACGCGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GG..CCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGG  >  NC_014034/2747749‑2747943 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG