Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,869	+G	100%	intergenic (‑201/+92)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,869	1	.→G	100.0%	56.2	22	intergenic (‑201/+92)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (20/1):  ref base (1/0):  total (21/1)

CGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GG..CCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTC  >  NC_014034/2747770‑2747962                                                                                                      |                                                                                                cgccccGCTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGcgcccgcc                                                                                                  <  2:1802792/98‑1 (MQ=21)   ccccGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                               <  1:747995/100‑1 (MQ=255)   ccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                                               <  2:188383/100‑1 (MQ=25)     ccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                                             <  2:2395303/101‑3 (MQ=25)     ccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                                             <  1:702708/101‑3 (MQ=25)      cgctTTCCCCCGCCCGCGGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                                            <  2:4142621/101‑4 (MQ=21)      cgctTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                                            <  2:4133233/101‑4 (MQ=21)          tccccccGCCCGCAGCGCCCGGTTGCGGCAGGACCGCTTCAGCCCGCACCACAGGGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgggc                                                                                        <  1:3237609/98‑8 (MQ=21)           ccccccGCCCGCAGCGCCCGGTTGCGGCAGGACCGCTTCACCCCGCCCCACAGAGGGCAGCGCCCGGCCCGGCGGGCGAGAAGCGCCCgcc.gggccgggcg                                                                                       <  1:4116955/99‑9 (MQ=18)                                                                       cgcCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGc                          >  2:4142135/1‑101 (MQ=18)                                                                        gcccggcccgGCGGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg                         >  1:490430/1‑101 (MQ=18)                                                                          ccggcccgGCGGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGAt                       >  1:1645388/1‑101 (MQ=18)                                                                            ggcccgGCGGGCGAGAAGCGCCCGCC.GGGACCGGC.GCTGCCCGAGGGCCTTTGGGCCCTCGGGGGGCGCAGGCCCTCTGTGACGCATGGCCAAGGCGATgg                     >  1:3661614/1‑101 (MQ=18)                                                                              cccggcgGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGcc                   >  2:1361967/5‑101 (MQ=18)                                                                               ccggcgGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCACGGCGATGGCCt                  >  2:3374190/4‑101 (MQ=18)                                                                                   cgGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGCTGGCCTTGGc              >  2:1885540/1‑101 (MQ=21)                                                                                    ggggggggaGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc             <  1:4194455/93‑1 (MQ=21)                                                                                    gggcGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc             >  2:1409004/4‑101 (MQ=21)                                                                                    gggcGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc             >  1:119572/4‑101 (MQ=21)                                                                                     ggcGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCa            >  2:1498111/3‑101 (MQ=21)                                                                                     ggcGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCa            >  1:3600466/3‑101 (MQ=21)                                                                                        gagaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg         >  1:475572/1‑101 (MQ=21)                                                                                        gagaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg         >  1:198/1‑101 (MQ=21)                                                                                         agaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGt        >  1:3857312/1‑101 (MQ=21)                                                                                         agaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGt        >  1:3026178/1‑101 (MQ=21)                                                                                         agaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGt        >  1:2458491/1‑101 (MQ=21)                                                                                         agaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGt        >  2:4325000/1‑101 (MQ=21)                                                                                          gaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTc       >  2:376633/1‑101 (MQ=21)                                                                                          gaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTc       >  2:4235120/1‑101 (MQ=21)                                                                                          gaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGGCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAggcg       >  2:2084552/1‑99 (MQ=21)                                                                                           aaGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCa      >  2:430159/1‑101 (MQ=21)                                                                                               gcccgccgGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCATAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTc  >  1:1958903/1‑101 (MQ=18)                                                                                                      |                                                                                                CGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GG..CCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTC  >  NC_014034/2747770‑2747962 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG