Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,780,410 +T 2.4% coding (980/1257 nt) RCAP_rcc02591 ← surface presentation of antigens protein family

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,780,4101.→T 2.4% 9.0 ‑2.9 ‑0.3165coding (980/1257 nt)RCAP_rcc02591surface presentation of antigens protein family
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (76/85):  total (80/85)
Fisher's exact test strand distribution p-value = 5.31e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.71e-01

Only 100 of 181 total aligned reads displayed.

CAGACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGCCGCACCGCGCG  >  NC_014034/2780310‑2780510
                                                                                                     |                                                                                                      
cAGACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGc                                                                                                         >  1:2148074/1‑101 (MQ=255)
 aGACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.t                                                                                                       <  1:2112144/101‑1 (MQ=255)
 aGACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.t                                                                                                       >  1:2589194/1‑101 (MQ=255)
  gACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.tt                                                                                                      <  1:537284/101‑1 (MQ=255)
   aCCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.ttt                                                                                                     >  1:3875286/1‑100 (MQ=255)
        cgccACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGcccc                                                                                                >  1:3730395/1‑101 (MQ=255)
        cgccACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGcccc                                                                                                <  1:953923/101‑1 (MQ=255)
          ccACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGGCCCGc                                                                                              >  1:110199/1‑101 (MQ=255)
           cACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTTCCCCCcc                                                                                             >  1:428636/1‑101 (MQ=255)
           cACTGCCGGGCAGCGGATCATAGGCCGGGGAATCAATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGcc                                                                                             <  1:3826236/101‑1 (MQ=255)
            aCTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCt                                                                                            <  1:1611021/101‑1 (MQ=255)
                ccGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCCCCCCgca                                                                                        >  2:4185305/1‑99 (MQ=255)
                 cGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCCCCCCC.AAg                                                                                      >  1:103506/1‑101 (MQ=255)
                  gggCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAgg                                                                                     >  2:2429596/1‑101 (MQ=255)
                   ggCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTGGCCCCCCCCCgcggg                                                                                      >  1:1942452/1‑97 (MQ=255)
                     cAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGggcg                                                                                  <  2:445402/101‑1 (MQ=255)
                     cAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGggcg                                                                                  <  1:719203/101‑1 (MQ=255)
                      aGCGGATCATAGGCCGGGGAAGCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTTCCCCCCCCCGCAAggggg                                                                                  >  1:2545972/1‑99 (MQ=255)
                       gCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTTGCGCCCCCCCG.AAGggggg                                                                                 >  2:526109/1‑101 (MQ=255)
                       gCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGggcggc                                                                                <  1:1766289/101‑1 (MQ=255)
                          gATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGggcggcggc                                                                             <  1:1021354/101‑1 (MQ=255)
                           aTCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCCCCCCC.AAAgggggcgggc                                                                            >  2:3874365/1‑99 (MQ=255)
                            tCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCac                                                                           <  2:2316077/101‑1 (MQ=255)
                            tCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCCCG.GAGggggggggcgc                                                                           >  2:1791447/1‑99 (MQ=255)
                             cATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGGCCCCCCCCC.CAGGGGGGCCGCagc                                                                          >  2:2690495/1‑99 (MQ=255)
                              aTAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCacga                                                                         <  2:3062614/101‑1 (MQ=255)
                               tAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCCCCTCG.CAGGGGGGGGGCacggc                                                                        >  2:3621716/1‑99 (MQ=255)
                               tAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCCCG.AAGGGCGGGGGGccgac                                                                        >  2:1249716/1‑101 (MQ=255)
                                aGGCCGGGGAATGGATCCGGTGGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCacgacg                                                                       <  2:3794588/101‑1 (MQ=255)
                                aGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCacgacg                                                                       <  1:2426395/101‑1 (MQ=255)
                                 ggCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCCCCCCC.AAGGGCGGCGGCacgccgg                                                                      >  1:769592/1‑100 (MQ=255)
                                  gCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGTCCCCCCCCC.CAGGGGGGCGGCCCGAcgcg                                                                     >  1:3655714/1‑101 (MQ=255)
                                  gCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGAcgcg                                                                     <  2:1102525/101‑1 (MQ=255)
                                     ggggAATCGATCCGGTCGAGCGGCGCGCGGGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCCCG.AAGGGCGGCGGCAAGACggggcg                                                                  >  2:2919240/1‑101 (MQ=255)
                                     ggggAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGGCCCCCCCCG.AAGGGGGGCGGGGCGGCgcggcg                                                                  >  1:1324561/1‑101 (MQ=255)
                                      gggAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGgcg.c                                                                <  2:3202719/101‑1 (MQ=255)
                                       ggAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.Ca                                                               <  1:4225362/101‑1 (MQ=255)
                                        gAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGGACCACGCGGCG.CAc                                                              >  2:2047692/1‑101 (MQ=255)
                                          aTCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGGCCCGCCTCC.GAGGGCGGCGGGACGAGCCGGCG.CACCt                                                            >  2:1815619/1‑101 (MQ=255)
                                          aTCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCGCCTCC.CAGGGGGGCGGGCCGACGCGGGG.CAccc                                                            >  1:3844451/1‑100 (MQ=255)
                                          aTCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCt                                                            <  2:3166612/101‑1 (MQ=255)
                                          aTCGATCCGGTCGAGCGGCGCCCGGGGCCCGCCGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCCCCCCG.GAAGGCCGGGGCACGACGCGGGG.CGCCt                                                            >  2:627615/1‑101 (MQ=255)
                                           tCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCCCG.AAAGGCGGGGGGCCGACGCGGGG.CCccct                                                           >  1:3919305/1‑99 (MQ=255)
                                            cGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCGCCCCG.AAGGGCGGCGGCGCGGCGCGGCG.CCCCttc                                                          >  2:2804794/1‑101 (MQ=255)
                                            cGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCttc                                                          <  2:2560150/101‑1 (MQ=255)
                                             gATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCttcg                                                         <  1:2096147/101‑1 (MQ=255)
                                                ccGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCttcggtt                                                      <  2:1324464/101‑1 (MQ=255)
                                                 cGGTCGAGCGGGGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCttcggttc                                                     <  2:3181374/101‑1 (MQ=255)
                                                 cGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCGCCCTCTCG.GAGGGGGGCGGCCGGGCGCGGCC.CCCCctcgggtc                                                     >  2:752644/1‑101 (MQ=255)
                                                 cGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCttcggttc                                                     <  1:2171958/101‑1 (MQ=255)
                                                 cGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCCCCTCC.AAGGGCGGCGGGGCGACGCGGCG.CAccctgcggtc                                                     >  2:593677/1‑93 (MQ=255)
                                                  ggTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTTCCCCCCCCCC.CAGGGGGGGGGGGCGCCGCGGCG.CCCCctcggttcg                                                    >  1:2872823/1‑101 (MQ=255)
                                                    tCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCggg                                                  <  1:2048305/101‑1 (MQ=255)
                                                     cGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGGCCCCCCTCC.CAGGGCGGGGGGCCCACCCGGGG.CACCCTCGGGTTgggg                                                 >  2:4216159/1‑100 (MQ=255)
                                                     cGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCGCCCCG.CAGGGGGGGGGCGCGACCCGGCG.CGCCctcgggtcgcgg                                                 >  2:3332250/1‑98 (MQ=255)
                                                     cGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCCCCCCG.AAGGGGGGGGGGGCGGCGCGGCG.CCCCTTCCGGTCgggg                                                 >  2:950185/1‑100 (MQ=255)
                                                      gAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCGCCTCG.GAGGGCGGCGGGACGACGCGGCG.GCCCTTTTGTTCggggg                                                >  2:4022038/1‑99 (MQ=255)
                                                      gAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGggcg                                                <  2:3844198/101‑1 (MQ=255)
                                                       atcggcgCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGggcgg                                               <  2:1380627/99‑1 (MQ=255)
                                                       aGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGggcgg                                               <  1:1358523/101‑1 (MQ=255)
                                                           gcgcgcgcGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGcc                                           <  2:3875032/101‑1 (MQ=255)
                                                            cgcgcgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCa                                          <  1:3023001/101‑1 (MQ=255)
                                                             gcgcgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGGGGCGGCACGACGCGGCG.CCCCTTCGGGTCGGGGGGCGCCAt                                         >  1:981133/1‑101 (MQ=255)
                                                             gcgcgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCAt                                         <  1:862368/101‑1 (MQ=255)
                                                             ccgcgcgGCCCGCTGGGCGCCGTTTTCCGGGCAAAGGGGC.TTGTCCCCTCCTCG.AAGGGCGGCGCCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCAt                                         <  2:1486845/100‑1 (MQ=255)
                                                              cgcgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGGCCCGCCCCC.AAGGGCGGCGGCACGCCGCCGCG.CCCCCTCGGGTCCggggggggccgc                                        >  1:4179992/1‑94 (MQ=255)
                                                              cgcgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCGCCCCG.AAAGGGGGCGGCACGCCGCGGGGCCCCCCCCGGTTCGggggggccccc                                         >  1:3121908/1‑95 (MQ=255)
                                                               gcgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCa                                       <  1:3458827/101‑1 (MQ=255)
                                                               gcgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCa                                       <  2:428596/101‑1 (MQ=255)
                                                                cgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAg                                      <  1:2075923/101‑1 (MQ=255)
                                                                  cgGCCGGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTCGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGct                                    <  2:504160/101‑1 (MQ=255)
                                                                   ggCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGctc                                   <  1:1157787/101‑1 (MQ=255)
                                                                    gCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCCCCCCC.GAGGGCGGCGGCCCGACGCGGCG.CCCCTTCCGTTCGGGGGGCGCCCCCAGctct                                  >  1:12932/1‑101 (MQ=255)
                                                                       cGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCGCCCCG.GAGGGGGGCGGGACGGCGCGGCG.CAGCCTCGGGTCGGGCGGGGCCCGCAGCccctct                               >  2:1719483/1‑101 (MQ=255)
                                                                       cGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCtcttct                               <  1:903509/101‑1 (MQ=255)
                                                                         cTGGGCGCCGATTTCGCGGCAAAGGGGC.TTTGCCCCGCCTCG.AAGGGGGGCGGCACGACGCGGCG.CACCTTCGGGTCGGGCGGGGCCACCAGCTcttcttt                             >  1:3751005/1‑100 (MQ=255)
                                                                          tGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCGCCCCG.AAGGGGGGGGGGACGACGCGGGG.CACCCTCGGTTCGGGCGGGGCCCTCCGCCcctctttc                            >  1:837388/1‑99 (MQ=255)
                                                                          tGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCCCCTCG.GAGGGGGGGGGGACGACGCGGGG.CACCCTCGGTTCGGGCGGGGGCAACCGCTcctctttc                            >  2:2120183/1‑99 (MQ=255)
                                                                          tGGGCGCCGATTTCCCGGCAAAGGGGC.TTTGCCCCCCCCCG.CAGGGGGGCGGGACGACGCGGGG.CACCCTCCGGTTGGGGGGGGCCACCCGCCcttttttc                            >  2:785768/1‑99 (MQ=255)
                                                                          tGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTcc                            <  2:2255013/101‑1 (MQ=255)
                                                                           gggCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCt                           <  2:264448/101‑1 (MQ=255)
                                                                           gggCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCt                           <  1:1479430/101‑1 (MQ=255)
                                                                             gcgcCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCg                         <  1:144356/101‑1 (MQ=255)
                                                                               gcCGATTTCCCGGCAAAGGGGC.TTTGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGGGGCGCCATCAGCCCTTCTTCCTCctg                       >  1:1744424/1‑101 (MQ=255)
                                                                               gcCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCgtg                       <  2:2159280/101‑1 (MQ=255)
                                                                                 cGATTTCCCGGCAAAGGGGCTTTGGCCCCCCCCCG..AGGGGGGGGGGACGACGCGGCG.CACCCTCCGGTCGGGGGGGGCCCTCCGCCCTTCTTCCTCGTGtt                     >  2:1756320/1‑101 (MQ=255)
                                                                                   aTTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGGGGCACGACGCGGCG.CACCTTCGGTTCCGGGGGCGCCATCCGCTCTTCTTCCTCCTGGTcc                   >  2:2060317/1‑100 (MQ=255)
                                                                                   aTTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCg                   <  2:2569293/101‑1 (MQ=255)
                                                                                   aTTTCCCGGCAAAGGGGC.TTGGCCCCGCCCCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCCTCAGCTCTTTTTTCTCGTTTTCg                   >  1:1988303/1‑101 (MQ=255)
                                                                                    tttCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGGGCCCTCAGCTCTTCTTCCTCGTGTTCGa                  >  1:3235355/1‑101 (MQ=255)
                                                                                     ttCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGAt                 <  2:2463904/101‑1 (MQ=255)
                                                                                       cccGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCa               >  2:4270106/1‑101 (MQ=255)
                                                                                        ccGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAg              <  1:3990345/101‑1 (MQ=255)
                                                                                          ggCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCCGCTCTTCTTCCTCGTGTTCGATCAGcc            >  2:239300/1‑101 (MQ=255)
                                                                                           gCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGCCg           <  2:2872615/101‑1 (MQ=255)
                                                                                             aaaGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCTGGCGGGGCCATCCGCTCTTCTTCCTNGTGTTCGNtnnnnnnnn         >  2:598272/1‑93 (MQ=255)
                                                                                              aaGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGCCGCAc        >  1:2793665/1‑101 (MQ=255)
                                                                                               aGGGGC.TTGGCCCCGCCTCG.AAGGGGGGGGGGACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCCTCCGCCCTTCTTCCCCGGGTTCGATCAgacccccc       >  2:3029155/1‑94 (MQ=255)
                                                                                               aGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGCCGCAcc       <  2:2320419/101‑1 (MQ=255)
                                                                                               aGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGCCGCAcc       <  1:2690685/101‑1 (MQ=255)
                                                                                                     |                                                                                                      
CAGACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGGCCCCGCCTCG.AAGGGCGGCGGCACGACGCGGCG.CACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGCCGCACCGCGCG  >  NC_014034/2780310‑2780510

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG