Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 279,645 +A 3.0% intergenic (‑47/‑33) RCAP_rcc00242 ← / → dapD hypothetical protein/2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034279,6450.→A 3.0% 17.4 ‑0.4 ‑0.1230intergenic (‑47/‑33)RCAP_rcc00242/dapDhypothetical protein/2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase
Reads supporting (aligned to +/- strand):  new base (4/2):  ref base (117/106):  total (121/109)
Fisher's exact test strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.98e-01

Only 100 of 250 total aligned reads displayed.

TTTTGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAACCCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGCC  >  NC_014034/279545‑279742
                                                                                                                                                                                                      
ttttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAc                                                                                                   >  2:2121490/1‑101 (MQ=255)
 tttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAcc                                                                                                  >  1:2849776/1‑101 (MQ=255)
   tGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGCGGGGCTTTCCCCAACTATAGGGGGAAAAccaa                                                                                                >  2:2783243/1‑99 (MQ=255)
    gCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAccatg                                                                                               <  2:2004743/101‑4 (MQ=255)
      aTCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGaa                                                                                            <  1:3713491/101‑1 (MQ=255)
      aTCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGaa                                                                                            <  1:3306574/101‑1 (MQ=255)
       tCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAAt                                                                                           <  2:2650621/101‑1 (MQ=255)
        ccATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATg                                                                                          <  2:3529889/101‑1 (MQ=255)
         cATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCGTATCCTGCTCATTGCGCGAAGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGc                                                                                         >  1:3808641/1‑101 (MQ=255)
          atgatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGcc                                                                                        >  2:132919/1‑101 (MQ=255)
          atgatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGcc                                                                                        <  1:3433070/101‑1 (MQ=255)
           tgatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCa                                                                                       <  2:515032/101‑1 (MQ=255)
           tgatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCa                                                                                       <  1:3870808/101‑1 (MQ=255)
            gatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCAc                                                                                      <  2:1075370/101‑1 (MQ=255)
            gaggCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCAc                                                                                      <  2:2391099/98‑1 (MQ=255)
              tgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCACCTATAGGGGGAAAA.CCATGAATGCCACtt                                                                                    >  2:3292728/1‑101 (MQ=255)
               gCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTg                                                                                   <  2:784646/101‑1 (MQ=255)
               gCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTg                                                                                   >  1:1096332/1‑101 (MQ=255)
                cTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCTTGAATGCCACTTGa                                                                                  >  1:297004/1‑101 (MQ=255)
                cTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGa                                                                                  <  2:2048117/101‑1 (MQ=255)
                 tGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGTGGGAAAA.CCATGAATGccgccagaa                                                                                 <  2:145378/101‑8 (MQ=255)
                  gTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGaaa                                                                                <  1:1475855/101‑1 (MQ=255)
                   tCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAag                                                                               >  1:4326838/1‑101 (MQ=255)
                    cACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAaga                                                                              <  2:4231883/101‑1 (MQ=255)
                    cACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATCCCACTTGAAaga                                                                              >  2:1297458/1‑101 (MQ=255)
                      cGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAgg                                                                            >  1:1313131/1‑101 (MQ=255)
                       ggAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGaaaagatc                                                                           >  1:766153/1‑96 (MQ=255)
                         aaCGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGAcc                                                                         >  1:4197640/1‑101 (MQ=255)
                         aaCGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGAcc                                                                         <  1:557548/101‑1 (MQ=255)
                          aCGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCg                                                                        >  1:823802/1‑101 (MQ=255)
                           cGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGt                                                                       <  1:2959731/101‑1 (MQ=255)
                            gggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGtt                                                                      >  1:933180/1‑101 (MQ=255)
                            gggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGtt                                                                      <  2:814542/101‑1 (MQ=255)
                            gggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGtt                                                                      >  1:3151547/1‑101 (MQ=255)
                            gggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGtt                                                                      >  2:472272/1‑101 (MQ=255)
                              gTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTcc                                                                    <  1:1501163/101‑1 (MQ=255)
                              gTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTcc                                                                    >  2:3938088/1‑101 (MQ=255)
                                gggAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGa                                                                  >  2:4041012/1‑101 (MQ=255)
                                 ggAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGAt                                                                 >  1:2332321/1‑101 (MQ=255)
                                   aaCGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGt                                                               >  1:28038/1‑101 (MQ=255)
                                     cGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCg                                                             <  1:2002711/101‑1 (MQ=255)
                                      ggCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGa                                                            >  2:2607654/1‑101 (MQ=255)
                                      ggCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGa                                                            <  2:4326569/101‑1 (MQ=255)
                                        cTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAAc                                                          <  2:1190160/101‑1 (MQ=255)
                                        cTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAAc                                                          >  1:2602092/1‑101 (MQ=255)
                                        cTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAAc                                                          <  1:2480072/101‑1 (MQ=255)
                                         tttCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACg                                                         >  1:4091972/1‑101 (MQ=255)
                                           tCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGcc                                                       >  1:31104/1‑101 (MQ=255)
                                            cGTCTTTCATAACCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCa                                                      <  2:4179037/101‑1 (MQ=255)
                                             gTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCAt                                                     <  2:2601921/101‑1 (MQ=255)
                                              tCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATg                                                    >  2:3708234/1‑101 (MQ=255)
                                                 ttCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAAcagatcggaa                                                 >  1:667973/1‑92 (MQ=255)
                                                   cATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGa                                               >  1:798746/1‑101 (MQ=255)
                                                    atatCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGaa                                              >  2:3335372/1‑101 (MQ=255)
                                                    atatCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGaa                                              >  1:3860051/1‑101 (MQ=255)
                                                    atatCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGaa                                              >  2:288423/1‑101 (MQ=255)
                                                     tatCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAg                                             >  1:3260959/1‑101 (MQ=255)
                                                       tCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCt                                           >  1:442149/1‑101 (MQ=255)
                                                          tGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGcc                                        >  1:1355417/1‑101 (MQ=255)
                                                           gCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCa                                       >  2:1154496/1‑101 (MQ=255)
                                                           gCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCa                                       <  1:1221824/101‑1 (MQ=255)
                                                            cTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCAt                                      <  1:2896447/101‑1 (MQ=255)
                                                             tCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTTGAAGCTGCCATc                                     <  1:2430447/101‑1 (MQ=255)
                                                             tCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATc                                     >  2:3973216/1‑101 (MQ=255)
                                                             tCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATc                                     >  1:103169/1‑101 (MQ=255)
                                                              cATTGCTCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCg                                    <  2:2992732/101‑1 (MQ=255)
                                                              cATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCg                                    >  2:84027/1‑101 (MQ=255)
                                                              cATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCg                                    >  1:1694863/1‑101 (MQ=255)
                                                                ttGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGaa                                  <  1:2262535/101‑1 (MQ=255)
                                                                ttGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGaa                                  <  1:1850252/101‑1 (MQ=255)
                                                                ttGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGaa                                  >  1:1715879/1‑101 (MQ=255)
                                                                   agcgAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgcc                               <  1:1183767/100‑1 (MQ=255)
                                                                     cgAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgccgc                             >  2:3050571/1‑101 (MQ=255)
                                                                     cgAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCAGATGTCGAACGCCATTCTCGAAGCTGCCATCGAAgccgc                             >  1:1897203/1‑101 (MQ=255)
                                                                      gAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgccgcc                            >  2:3143343/1‑101 (MQ=255)
                                                                      gAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgccgcc                            <  2:223434/101‑1 (MQ=255)
                                                                        ggggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTg                          <  2:2955158/101‑1 (MQ=255)
                                                                          ggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTggg                        <  1:4041269/101‑1 (MQ=255)
                                                                          ggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTggg                        >  2:416468/1‑101 (MQ=255)
                                                                            cTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAg                      >  1:4163312/1‑101 (MQ=255)
                                                                             tttCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAgg                     >  1:1768302/1‑101 (MQ=255)
                                                                              ttCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGt                    >  2:794983/1‑101 (MQ=255)
                                                                              ttCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGt                    <  1:3050792/101‑1 (MQ=255)
                                                                               tCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGtt                   <  1:982150/101‑1 (MQ=255)
                                                                                ccccAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTc                  >  2:2822608/1‑101 (MQ=255)
                                                                                  ccAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTcgc                >  2:4007647/1‑101 (MQ=255)
                                                                                   cAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTcgcg               <  2:103148/101‑1 (MQ=255)
                                                                                   cAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTcgcg               >  2:1631625/1‑101 (MQ=255)
                                                                                    aaCTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGCCCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCGGGGAGGTCCGGGa              >  1:2071568/1‑101 (MQ=255)
                                                                                    aaCTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGa              >  1:46983/1‑101 (MQ=255)
                                                                                     aCTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGac             >  1:2760791/1‑101 (MQ=255)
                                                                                     aCTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGac             >  1:3552199/1‑101 (MQ=255)
                                                                                     aCTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGac             >  2:2740516/1‑101 (MQ=255)
                                                                                       tataGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGacac           <  2:2544535/101‑1 (MQ=255)
                                                                                        ataGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACAcc          >  1:2613693/1‑101 (MQ=255)
                                                                                        ataGGGGGAAAA.CCAGGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCGGCCTGGGAGGTTCGCGACAcc          >  1:3750161/1‑101 (MQ=255)
                                                                                         taGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACAccc         >  2:1866049/1‑101 (MQ=255)
                                                                                               ggaaaACCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACg    >  2:2367332/3‑101 (MQ=255)
                                                                                               ggaaaACCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACg    <  2:1878827/99‑1 (MQ=255)
                                                                                                gaaaACCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGc   <  1:1810723/100‑1 (MQ=255)
                                                                                                                                                                                                      
TTTTGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAACCCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGCC  >  NC_014034/279545‑279742

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG