Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,808,557 +G 100% intergenic (+112/+42) pyrD2 → / ← acsA2 dihydroorotate oxidase/acetate‑‑CoA ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,808,5571.→G100.0% 159.0 77intergenic (+112/+42)pyrD2/acsA2dihydroorotate oxidase/acetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  new base (48/28):  ref base (1/0):  total (49/28)

CTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGA.GTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGCCTCCGGTCAGGCCCGGTTCATCCGGTTGTCGATCAGCTCCTGCACCACGCCCGGA  >  NC_014034/2808457‑2808647
                                                                                                     |                                                                                          
cTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGgaga                                                                                             <  1:4332884/101‑1 (MQ=38)
cTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGgaga                                                                                             <  2:653778/101‑1 (MQ=38)
 tGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.g                                                                                           <  2:1209383/101‑1 (MQ=37)
  gCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.gg                                                                                          <  1:247780/101‑2 (MQ=21)
   cGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggt                                                                                         <  1:545287/101‑3 (MQ=18)
   cGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggt                                                                                         <  2:585776/101‑3 (MQ=18)
   cGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggg                                                                                         >  1:437820/1‑99 (MQ=21)
    gggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggtg                                                                                        <  2:2919264/101‑4 (MQ=17)
     ggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGgga.ggtgg                                                                                       >  2:2110546/1‑97 (MQ=9)
     ggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggtgg                                                                                       >  2:4161494/1‑97 (MQ=17)
     ggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggtgg                                                                                       <  2:3786572/101‑5 (MQ=14)
     ggggATTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggtgg                                                                                       >  1:760969/1‑97 (MQ=9)
       ggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggtggca                                                                                     <  2:229708/101‑7 (MQ=2)
        gTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGgcgc                                                                                     >  1:2634365/1‑101 (MQ=2)
        gTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGgcgc                                                                                     >  1:617794/1‑101 (MQ=2)
         tttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCa                                                                                    <  1:3382779/101‑1 (MQ=2)
         tttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCa                                                                                    <  2:719948/101‑1 (MQ=2)
         tttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCa                                                                                    >  1:433541/1‑101 (MQ=2)
         tttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGA.GGgggcgca                                                                                   >  1:131055/1‑96 (MQ=9)
         tttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGA.GGgggcgca                                                                                   >  2:892030/1‑96 (MQ=12)
          ttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAg                                                                                   >  2:357817/1‑101 (MQ=9)
          ttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAg                                                                                   <  2:873714/101‑1 (MQ=9)
          ttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAg                                                                                   >  1:4250359/1‑101 (MQ=12)
           tCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAgg                                                                                  >  1:2272461/1‑101 (MQ=16)
           tCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAgg                                                                                  >  1:3298947/1‑101 (MQ=12)
            cacaCCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGc                                                                                 <  2:696838/101‑1 (MQ=12)
            cacaCCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGc                                                                                 <  2:1183270/101‑1 (MQ=12)
            cacaCCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGc                                                                                 <  1:752360/101‑1 (MQ=12)
             acccccccgctcccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCa                                                                                <  1:651672/98‑1 (MQ=11)
             acacCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCa                                                                                >  1:1006789/1‑101 (MQ=16)
             acacCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCa                                                                                >  1:1363297/1‑101 (MQ=16)
             acacCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCa                                                                                >  1:3227046/1‑101 (MQ=16)
               acccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAg                                                                              >  2:3531158/1‑101 (MQ=19)
               acccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAg                                                                              <  1:2442170/101‑1 (MQ=12)
               acccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAg                                                                              >  2:196360/1‑101 (MQ=19)
               acccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAg                                                                              <  2:1935326/101‑1 (MQ=12)
                  cccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCg                                                                           >  1:4097995/1‑101 (MQ=19)
                  cccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCg                                                                           >  2:3517668/1‑101 (MQ=19)
                  cccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCg                                                                           >  1:812313/1‑101 (MQ=19)
                  cccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCg                                                                           >  1:3012246/1‑101 (MQ=19)
                    cgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCggg                                                                         >  1:524485/1‑101 (MQ=19)
                    cgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCggg                                                                         >  1:4004766/1‑101 (MQ=19)
                     gcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCgggg                                                                        >  2:3746458/1‑101 (MQ=19)
                     gcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCgggg                                                                        >  2:454692/1‑101 (MQ=16)
                     gcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCgggg                                                                        >  2:117469/1‑101 (MQ=16)
                     gcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTAGCGCAGGCAAGTCgggg                                                                        >  2:1301982/1‑101 (MQ=255)
                      cacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCggggg                                                                       >  2:2868585/1‑101 (MQ=16)
                      cacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCggggg                                                                       <  2:2304176/101‑1 (MQ=16)
                       acccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCgggggg                                                                      <  2:3634316/101‑1 (MQ=16)
                        cccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggc                                                                     <  2:833810/101‑1 (MQ=16)
                        cccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggc                                                                     >  2:1983718/1‑101 (MQ=12)
                        cccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggc                                                                     <  1:2871449/101‑1 (MQ=12)
                         ccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAACTCTCCCGGGGGGAGAGGTGGCGCAGGCCAGTCGGGGggcg                                                                    >  2:1654762/1‑101 (MQ=11)
                         ccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcg                                                                    >  1:460764/1‑101 (MQ=16)
                         ccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcg                                                                    <  2:4020576/101‑1 (MQ=16)
                         ccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcg                                                                    <  2:2070295/101‑1 (MQ=16)
                         ccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcg                                                                    <  1:3399683/101‑1 (MQ=12)
                         ccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcg                                                                    <  2:627128/101‑1 (MQ=16)
                          cccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcgg                                                                   >  2:1801842/1‑101 (MQ=16)
                          cccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcgg                                                                   <  2:2372562/101‑1 (MQ=16)
                           ccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcggc                                                                  <  1:3719185/101‑1 (MQ=16)
                           ccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcggc                                                                  <  2:4130868/101‑1 (MQ=12)
                           ccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcggc                                                                  >  2:251837/1‑101 (MQ=12)
                             gTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGgcgc                                                                >  2:3386561/1‑101 (MQ=16)
                             gTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGgcgc                                                                >  2:4029539/1‑101 (MQ=16)
                               ggCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccc                                                              <  2:1159361/101‑1 (MQ=12)
                               ggCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccc                                                              >  1:2841970/1‑101 (MQ=16)
                               ggCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccc                                                              >  1:1196094/1‑101 (MQ=16)
                               ggCGGATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccc                                                              >  2:3764551/1‑101 (MQ=255)
                                gCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccc                                                             <  2:2733734/101‑1 (MQ=12)
                                gCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccc                                                             >  2:816953/1‑101 (MQ=16)
                                 cGTATTTGCGCCCAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc                                                            >  1:2423077/1‑101 (MQ=255)
                                 cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc                                                            <  2:4097735/101‑1 (MQ=12)
                                 cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc                                                            >  1:462577/1‑101 (MQ=16)
                                 cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc                                                            <  2:2823212/101‑1 (MQ=12)
                                 cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc                                                            >  2:2385641/1‑101 (MQ=16)
                                  gTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccccc                                                           <  1:2701913/101‑1 (MQ=12)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCGCCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg                                                          <  1:398494/101‑1 (MQ=255)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg                                                          >  1:4189162/1‑101 (MQ=12)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg                                                          >  1:1154660/1‑101 (MQ=12)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg                                                          <  1:2237387/101‑1 (MQ=12)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg                                                          >  1:2872155/1‑101 (MQ=12)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg                                                          >  2:2268402/1‑101 (MQ=12)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg                                                          >  2:3144385/1‑101 (MQ=12)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg                                                          >  2:355604/1‑101 (MQ=12)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg                                                          <  1:2689716/101‑1 (MQ=12)
                                    aTTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGGGGCGCCCCCCGc                                                         >  1:266396/1‑101 (MQ=9)
                                    aTTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGc                                                         >  2:2628604/1‑101 (MQ=9)
                                    aTTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGc                                                         >  1:636587/1‑101 (MQ=9)
                                    aTTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGGGGCGCAGGCAAGTCGGGGGGGGGGGGCCCCCGc                                                         >  2:1822602/1‑101 (MQ=11)
                                                                                          cgggggggGGG.GGGGCCCAGGCAGGTCGGGGGGCGGCGCCCCCCGCCTCCGGTCAGGCCCGGTTCATCCGGTTGTCGATCAGCTCCTGCACCACGCCCGGa  <  2:1709747/99‑1 (MQ=11)
                                                                                           cGGGGGTAGAGGAGGCGCAGGCAAGTCGGGGGGGGGCGGCCCCCGCCTCCGCCCAGGCCCGGATCATCCGGGCTTCGATCAGCTCCTGCTCCACGCCGGGa  >  2:1068949/1‑101 (MQ=255)
                                                                                                     |                                                                                          
CTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGA.GTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGCCTCCGGTCAGGCCCGGTTCATCCGGTTGTCGATCAGCTCCTGCACCACGCCCGGA  >  NC_014034/2808457‑2808647

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG