Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,880,582 +G 100% coding (1003/1053 nt) pdxA ← 4‑hydroxythreonine‑4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,880,5821.→G100.0% 36.7 23coding (1003/1053 nt)pdxA4‑hydroxythreonine‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (21/2):  ref base (0/0):  total (21/2)

CAAGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCC.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCGGG  >  NC_014034/2880484‑2880634
                                                                                                             |                                                    
cAAGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCg.cg                                                    >  2:2896521/1‑101 (MQ=255)
cAAGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCg.cg                                                    >  2:2391693/1‑101 (MQ=255)
 aaGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCg.cgt                                                   >  2:795088/1‑100 (MQ=255)
 aaGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCg.cgt                                                   >  1:3201459/1‑100 (MQ=255)
   gggCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCg.cgtca                                                 >  2:2185637/1‑98 (MQ=255)
    ggcggcAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCg.cgtcac                                                >  2:1042544/1‑97 (MQ=255)
    ggcggcAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCg.cgtcac                                                >  1:545642/1‑97 (MQ=255)
       ggcAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCg.cgtcacagc                                             >  1:1266681/1‑94 (MQ=255)
         cAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCg.cgtcacagccg                                           >  2:1890414/1‑92 (MQ=25)
                                                 cATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATc              >  2:3552354/1‑101 (MQ=9)
                                                 cATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATc              >  2:2313433/1‑101 (MQ=9)
                                                 cATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATc              >  2:2126126/1‑101 (MQ=9)
                                                 cATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGGCAAGGCCGCGATc              >  2:3929105/1‑101 (MQ=2)
                                                  ctCTTCCTTCAAATACCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCa             <  2:2120557/100‑1 (MQ=9)
                                                  aTCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCa             >  1:1486681/1‑101 (MQ=9)
                                                  aTCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCa             >  2:2872736/1‑101 (MQ=9)
                                                   tCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATccg            >  2:2483284/1‑99 (MQ=9)
                                                    cTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGa           >  1:2732462/1‑101 (MQ=9)
                                                    cTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGa           >  2:1797565/1‑101 (MQ=9)
                                                     ttGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGAc          >  1:3616674/1‑101 (MQ=9)
                                                     ttGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGAc          >  2:2180277/1‑101 (MQ=9)
                                                       gCTTCAAATATCCGGGGGGTCGGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACtg        <  1:2185776/101‑1 (MQ=12)
                                                        cTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACtgt       >  1:2041846/1‑101 (MQ=12)
                                                        cTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACtgt       >  2:3713978/1‑101 (MQ=12)
                                                         ttCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACtgtg      >  1:704137/1‑101 (MQ=12)
                                                         ttCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACtctg      >  2:1446721/1‑101 (MQ=9)
                                                          tCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCCTCGTCAAGGCCGCGATCAGACTGTGc     >  1:1342240/1‑101 (MQ=9)
                                                           cAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGGCGCGATCAGACTGTGCg    >  2:4010299/1‑101 (MQ=9)
                                                           cAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCg    >  1:2577397/1‑101 (MQ=12)
                                                            aaaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCgg   >  1:2788940/1‑101 (MQ=12)
                                                            aaaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCgg   >  1:1920837/1‑101 (MQ=12)
                                                             aaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCggg  >  1:693873/1‑101 (MQ=12)
                                                                                                             |                                                    
CAAGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCC.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCGGG  >  NC_014034/2880484‑2880634

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG