Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 296,280 +T 3.5% intergenic (‑80/‑203) RCAP_rcc00258 ← / → RCAP_rcc00259 lipoprotein/porin family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034296,2800.→T 3.5% 15.5 ‑2.7 ‑0.0225intergenic (‑80/‑203)RCAP_rcc00258/RCAP_rcc00259lipoprotein/porin family protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (138/79):  total (139/86)
Fisher's exact test strand distribution p-value = 6.62e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.01e-01

Only 100 of 251 total aligned reads displayed.

CAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTCCCGCCGGTCCCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCG  >  NC_014034/296180‑296379
                                                                                                                                                                                                        
cAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTc                                                                                                     >  1:3870850/1‑101 (MQ=255)
 aaaGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcc                                                                                                    >  1:871044/1‑101 (MQ=255)
   agagGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccc                                                                                                  <  1:2511481/101‑1 (MQ=255)
    cagGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTccccc                                                                                                 >  1:3684075/2‑101 (MQ=255)
     agGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccg                                                                                                >  2:4056992/1‑100 (MQ=255)
     agGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccc                                                                                                >  1:3131582/1‑101 (MQ=255)
      ggTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgc                                                                                               >  2:2043442/1‑99 (MQ=255)
          gCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGGTTTTT.CCCCCNCGGGa                                                                                          >  1:1174463/1‑101 (MQ=255)
           cgttcttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAc                                                                                         <  2:3570418/99‑1 (MQ=255)
           cTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAc                                                                                         <  1:1540215/101‑1 (MQ=255)
            tttCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAcc                                                                                        <  2:2173433/101‑1 (MQ=255)
            tttCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAcc                                                                                        >  1:1667160/1‑101 (MQ=255)
            tttCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAcc                                                                                        <  2:1864069/101‑1 (MQ=255)
             tnnnnnnaccGATCGACNGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccg                                                                                       <  2:1141186/92‑1 (MQ=255)
              tcttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgc                                                                                      >  1:2171003/1‑101 (MQ=255)
               cttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcc                                                                                     >  1:2976532/1‑101 (MQ=255)
               cttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcc                                                                                     >  2:4032781/1‑101 (MQ=255)
               cttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcc                                                                                     >  2:1028945/1‑101 (MQ=255)
                ttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgccc                                                                                    >  1:2807346/1‑101 (MQ=255)
                 tcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccg                                                                                   >  1:332178/1‑101 (MQ=255)
                 tcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccc                                                                                   >  1:2246701/1‑100 (MQ=255)
                  cATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccgc                                                                                  >  1:2484411/1‑101 (MQ=255)
                   aTACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCcgcg                                                                                 <  1:1604059/101‑1 (MQ=255)
                       gATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGcggcgg                                                                             >  2:1656814/1‑101 (MQ=255)
                       gATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGcggcgg                                                                             >  2:2839609/1‑101 (MQ=255)
                        aTCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGGGACAAGATTTTT.CCCCCGCGGGACGGCCCGCGGCggg                                                                            >  2:3394752/1‑101 (MQ=255)
                           gACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGgcg                                                                         <  2:1268480/101‑1 (MQ=255)
                           gACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGgcg                                                                         >  2:4202251/1‑101 (MQ=255)
                            aCTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGgcgc                                                                        >  2:2268752/1‑101 (MQ=255)
                              tGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCaa                                                                      >  2:3416678/1‑101 (MQ=255)
                              tGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCaa                                                                      >  1:2011306/1‑101 (MQ=255)
                               gCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAAc                                                                     >  2:2439477/1‑101 (MQ=255)
                               gCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCCGCGGGGCGCAAc                                                                     >  1:2732595/1‑101 (MQ=255)
                                 ccccGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGCCCGCCCGCGGCGGGGCGCAAccc                                                                   >  2:50926/1‑100 (MQ=255)
                                   ccGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTcc                                                                 <  2:2783266/101‑1 (MQ=255)
                                    cGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccg                                                                >  2:2746960/1‑100 (MQ=255)
                                      cAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgcc                                                              >  2:2362000/1‑98 (MQ=255)
                                      cAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgcc                                                              >  2:1369748/1‑98 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTGCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                             >  1:2540593/1‑97 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                             >  2:2111824/1‑97 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                             >  2:1210873/1‑97 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTccgccg                                                             >  1:1675270/1‑97 (MQ=255)
                                         gCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGt                                                          >  2:4145800/1‑101 (MQ=255)
                                          cTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTc                                                         >  2:3818219/1‑101 (MQ=255)
                                          cTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTc                                                         >  1:1003116/1‑101 (MQ=255)
                                           tGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGGAACCT.CCGCCGGTcc                                                        >  2:551641/1‑101 (MQ=255)
                                           tGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcc                                                        >  2:986620/1‑101 (MQ=255)
                                            gAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccc                                                       >  1:2928705/1‑101 (MQ=255)
                                             aaCCCATTGGTTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccca                                                      <  1:381182/101‑2 (MQ=255)
                                             aaCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccca                                                      <  2:4378821/101‑2 (MQ=255)
                                             aaCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTccca                                                      >  1:3623423/1‑100 (MQ=255)
                                              ccccATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcccag                                                     <  1:106579/100‑3 (MQ=255)
                                              aCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcccag                                                     <  1:738955/101‑3 (MQ=255)
                                               cccATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcccagc                                                    >  1:4113232/1‑98 (MQ=255)
                                                ccATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGTcccagcg                                                   >  2:1808345/1‑97 (MQ=255)
                                                    tGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCggggg                                              >  1:1343697/1‑101 (MQ=255)
                                                    tGGCTTTTCCTGCCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCggggg                                              <  2:1524042/101‑1 (MQ=255)
                                                     ggCTTTTCCTGTCTCTAGCCGATGGCGGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGa                                             <  2:670252/101‑1 (MQ=255)
                                                     ggCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGa                                             >  2:458509/1‑101 (MQ=255)
                                                      gCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGAc                                            >  2:960994/1‑101 (MQ=255)
                                                       cTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGGCt                                           >  1:2278060/1‑101 (MQ=255)
                                                          ttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACtggg                                        >  1:1282135/1‑99 (MQ=255)
                                                            ccTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGc                                      <  1:221680/101‑1 (MQ=255)
                                                             cagcctccAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGAT.CCCAGCGGGGGACTGTGGCa                                     <  1:3294237/97‑1 (MQ=255)
                                                             cTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGCCTGTGGCa                                     >  2:2487089/1‑101 (MQ=255)
                                                             cTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCa                                     <  1:2747150/101‑1 (MQ=255)
                                                              tGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaa                                    >  2:161183/1‑101 (MQ=255)
                                                               gTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaaa                                   >  2:820984/1‑101 (MQ=255)
                                                               gTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaaa                                   <  1:2061794/101‑1 (MQ=255)
                                                                tctcTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGGCTGTGGCAAAg                                  >  1:3410878/1‑101 (MQ=255)
                                                                 ctctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGc                                 >  2:4003931/1‑101 (MQ=255)
                                                                 ctctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGc                                 >  2:1630909/1‑101 (MQ=255)
                                                                 ctctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCACCGGGGGACTGTGGCAAAGc                                 >  2:2849452/1‑101 (MQ=255)
                                                                   ctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGTCCGCCCGCGGCGGGGCGCAACC.CCCGGCGG.ACCCAGCCGGGGCTTGTGGCACAGCTg                               >  2:2922674/1‑101 (MQ=255)
                                                                   ctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTg                               >  2:3961465/1‑101 (MQ=255)
                                                                    tAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGc                              >  2:324369/1‑101 (MQ=255)
                                                                    tAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGc                              >  1:615018/1‑101 (MQ=255)
                                                                    tAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGc                              >  1:1725777/1‑101 (MQ=255)
                                                                    tAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGc                              >  1:3520188/1‑101 (MQ=255)
                                                                     aGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGTCCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGca                             <  1:2986865/101‑1 (MQ=255)
                                                                     aGCCGATGGCCGGGGGGGCGACAAGA.TTTTCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGca                             <  1:4146061/101‑1 (MQ=255)
                                                                      gCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcac                            >  2:3034099/1‑101 (MQ=255)
                                                                       ccGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTCCC.CCGGT.CCCAGCGGGGGACTGTGGGAAAGCTGcacc                           >  1:2351821/1‑101 (MQ=255)
                                                                       ccGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcacc                           >  1:2793652/1‑101 (MQ=255)
                                                                        cGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcacca                          <  1:551693/101‑1 (MQ=255)
                                                                         gATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGGCTGTGGCAAAGCTGcaccac                         >  1:1401059/1‑101 (MQ=255)
                                                                          aTGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCAcg                        <  2:2556198/101‑1 (MQ=255)
                                                                           tGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTTGCAAAGCTGCACCAcgc                       >  2:3493858/1‑101 (MQ=255)
                                                                                  gggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCg                <  2:332143/101‑1 (MQ=255)
                                                                                   ggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGc               >  1:1856898/1‑101 (MQ=255)
                                                                                   ggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGc               >  2:2893226/1‑101 (MQ=255)
                                                                                    gggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGTGACTGTGGCAAAGCTGCAGCACGCGGTTTCGCt              >  1:3843871/1‑101 (MQ=255)
                                                                                     ggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCtt             <  2:3131358/101‑1 (MQ=255)
                                                                                     ggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCtt             <  1:980119/101‑1 (MQ=255)
                                                                                       cGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTg           <  2:2540430/101‑1 (MQ=255)
                                                                                       cGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTg           <  2:1671272/101‑1 (MQ=255)
                                                                                        gACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGGGGCAAAGCTGCACCACGCGGGTTCGCTTTgg          >  2:793408/1‑101 (MQ=255)
                                                                                         aCAAGATTTTT.CCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTTGCAAAGCTGCACCACGCGGTTTCGCTTTGGc         <  1:3961721/101‑1 (MQ=255)
                                                                                         aCAAGA.TTCTCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGc         <  2:3683822/101‑1 (MQ=255)
                                                                                               attttTCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCtt    <  2:2081353/100‑1 (MQ=255)
                                                                                                                                                                                                        
CAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTCCCCCCGCGGGACCGCCCGCGGCGGGGCGCAACCTCCCGCCGGTCCCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCG  >  NC_014034/296180‑296379

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG