Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,173,548	+C	100%	intergenic (+583/‑99)	tyrA → / → RCAP_rcc02983	prephenate dehydrogenase/extensin family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,173,548	1	.→C	100.0%	271.3	73	intergenic (+583/‑99)	tyrA/RCAP_rcc02983	prephenate dehydrogenase/extensin family protein
Reads supporting (aligned to +/- strand):  new base (49/23):  ref base (0/1):  total (49/24)

CCCGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.G.CCGCCGCGCAAGCGCA.A.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTG  >  NC_014034/3173450‑3173642                                                                                                     |                                                                                                 cccGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.g.cc                                                                                                <  2:952209/101‑1 (MQ=255) cccGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.g.cc                                                                                                <  1:1500144/101‑1 (MQ=255)  ccGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.g.ccc                                                                                               >  1:3648115/1‑100 (MQ=255)  ccGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCCGTCGCGCCTTTTCG.g.ccc                                                                                               >  1:3651912/1‑100 (MQ=255)     gTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.g.cccgcc                                                                                            <  2:3043960/101‑5 (MQ=255)       cGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCcgccgc                                                                                           >  2:1902161/1‑101 (MQ=255)          cccgcccGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCgcgca                                                                                        <  2:2862029/96‑1 (MQ=255)          cTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCgcgca                                                                                        >  1:1024933/1‑101 (MQ=255)              gCCTCGCCGGCCCCCCCCCCCCCCGCCCCCACCCCCCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCgcgcaagcg                                                                                    <  1:3062409/101‑1 (MQ=255)              gCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCgcgcaagcg                                                                                    >  1:1963891/1‑101 (MQ=255)               ccGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCgcgcaagcgc                                                                                   <  1:3341172/101‑1 (MQ=255)                  cgcCGGTCACGCCCGCCCCCGCCCCCACCGCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCgcgcaagcgca.ac                                                                               <  1:4198847/101‑2 (MQ=255)                       gTCCCGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.A.Cgcccc                                                                         <  2:3302900/101‑1 (MQ=255)                        tcccccccgcccccgccCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGccccc                                                                         <  2:3233413/96‑1 (MQ=255)                        tCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGccccc                                                                         <  1:1998913/101‑1 (MQ=255)                        tCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGccccc                                                                         <  2:4447773/101‑1 (MQ=255)                           cgcccgccCCCGCCCCCACCCCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCccccgcc                                                                      <  2:3897393/101‑1 (MQ=255)                           cgcccgccCCCGCCCCCACCCCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCccccgcc                                                                      <  2:4091146/101‑1 (MQ=255)                              ccgcccccgcccCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCccccgccccg                                                                   >  1:2466598/1‑101 (MQ=255)                               cgcccccgccccCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCgg                                                                  >  1:3148041/1‑101 (MQ=255)                                   cccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgc                                                              >  1:3702609/1‑101 (MQ=255)                                    ccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcg                                                             >  2:170697/1‑101 (MQ=255)                                    ccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcg                                                             >  2:2861359/1‑101 (MQ=255)                                     cgcnccnCCCCCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcgc                                                            <  1:693662/101‑1 (MQ=255)                                     cgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcgc                                                            >  2:1059951/1‑101 (MQ=255)                                       cccccCCCACCCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcgccc                                                          <  1:2861567/101‑1 (MQ=255)                                         cccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcgcccgc                                                        >  2:3203897/1‑101 (MQ=255)                                          ccaccaCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcgcccgcg                                                       <  2:3147817/101‑1 (MQ=255)                                          ccaccaCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGCAAacgcccgcg                                                       >  2:370479/1‑101 (MQ=255)                                             ccccTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcgcccgcgccc                                                    <  2:2271227/98‑1 (MQ=255)                                              cacTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGcgcccgcgcccg                                                   >  1:722248/1‑101 (MQ=255)                                               acTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCCAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCCGCCGa                                                  >  2:81102/1‑101 (MQ=255)                                               acTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGa                                                  >  2:3519289/1‑101 (MQ=255)                                                cTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGaa                                                 >  1:2777402/1‑101 (MQ=255)                                                cTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGaa                                                 >  1:3308529/1‑101 (MQ=255)                                                 tCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAg                                                >  1:2250503/1‑101 (MQ=255)                                                 tCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAg                                                >  1:1788786/1‑101 (MQ=255)                                                 ccccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAg                                                <  2:3647862/100‑1 (MQ=255)                                                  cccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAGGCGCCCGCGCCCGAAGc                                               >  1:1472906/1‑101 (MQ=255)                                                  cccGCAGCCCGGAAGCCGCCGCCGGGCGGAGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCACC.GCCCCCGCCCCGGAAGCGCCCGCGCCCGCAGc                                               >  1:325567/1‑101 (MQ=255)                                                   ccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGcc                                              >  2:4091318/1‑101 (MQ=255)                                                    cGCAGCCCGGACGCCGCCGCCGGGCGGCGTGCTCTCGCGCCTTTTCG.GCCCGCCGCCCAAGCGCA.ACCCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGccc                                             <  1:4197055/101‑1 (MQ=255)                                                    cGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGccc                                             <  2:722168/101‑1 (MQ=255)                                                    cGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGccc                                             >  1:782018/1‑101 (MQ=255)                                                    cGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGccc                                             >  1:3639885/1‑101 (MQ=255)                                                     gCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGcccc                                            >  1:1940887/1‑101 (MQ=255)                                                      cAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCt                                           >  2:909754/1‑101 (MQ=255)                                                        ccccGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTcc                                         <  2:1175805/100‑1 (MQ=255)                                                          ccGGAAGCCGCCGCCGGGCGGCGTGCGGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCACC.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCgg                                       <  2:1472801/101‑1 (MQ=255)                                                            ggAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGcc                                     >  1:702474/1‑101 (MQ=255)                                                            ggAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGcc                                     <  1:4088994/101‑1 (MQ=255)                                                             gAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCCGccc                                    >  2:2693792/1‑101 (MQ=255)                                                              aaGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCg                                   >  2:1379384/1‑101 (MQ=255)                                                              aaGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCCGCCCg                                   >  2:3189376/1‑101 (MQ=255)                                                                  cgccgccgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAgg                               >  2:2167304/1‑101 (MQ=255)                                                                   tccgccgGGCGGCGTGCTTTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAggg                              <  2:1024848/100‑1 (MQ=255)                                                                      gccgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCg                           <  2:1411550/101‑1 (MQ=255)                                                                        cgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAg                         >  2:561971/1‑101 (MQ=255)                                                                         gggCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGc                        >  1:1670171/1‑101 (MQ=255)                                                                           gcgtctttcTGTCCCTCCTTTTCGCC.CCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGccc                      <  2:3651659/93‑1 (MQ=255)                                                                           gcggcgTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGccc                      <  2:4317926/101‑1 (MQ=255)                                                                               cgTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                  >  1:1568678/1‑101 (MQ=255)                                                                                gTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                 >  2:172950/1‑101 (MQ=255)                                                                                gTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                 >  2:2144794/1‑101 (MQ=255)                                                                                gTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                 >  2:2626796/1‑101 (MQ=255)                                                                                gTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGCGCCGAGCCCGGCCt                 >  1:4407058/1‑101 (MQ=255)                                                                                 tGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTg                >  1:2203422/1‑101 (MQ=255)                                                                                  gCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgc               >  2:344976/1‑101 (MQ=255)                                                                                   cTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcg              >  1:3404696/1‑101 (MQ=255)                                                                                    tGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcgc             >  1:1252541/1‑101 (MQ=255)                                                                                     gTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGcgcg            >  2:3997491/1‑101 (MQ=255)                                                                                      tCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCgg           >  2:1400277/1‑101 (MQ=255)                                                                                       cgcgCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCggg          >  2:4166906/1‑101 (MQ=255)                                                                                          gcCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggccg       >  1:3071123/1‑101 (MQ=255)                                                                                           ccTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcgg      >  1:692339/1‑101 (MQ=255)                                                                                            cTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcggt     >  1:270049/1‑101 (MQ=255)                                                                                            cTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGgggcggc     >  2:2033347/1‑100 (MQ=255)                                                                                              tttCG.GCCCGCCGCGCAAGCGCACC.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt   <  2:2250358/101‑1 (MQ=255)                                                                                              tttCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt   <  2:781938/101‑1 (MQ=255)                                                                                               ttCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGGCGGGCTg  >  2:1706526/1‑101 (MQ=255)                                                                                                     |                                                                                                 CCCGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.G.CCGCCGCGCAAGCGCA.A.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTG  >  NC_014034/3173450‑3173642 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG