Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,173,565 +C 95.9% intergenic (+600/‑82) tyrA → / → RCAP_rcc02983 prephenate dehydrogenase/extensin family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,173,5651.→C95.9% 2.2 ‑1.7 ‑1.173intergenic (+600/‑82)tyrA/RCAP_rcc02983prephenate dehydrogenase/extensin family protein
Reads supporting (aligned to +/- strand):  new base (52/18):  ref base (1/2):  total (53/20)
Fisher's exact test strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.64e-02

CGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.G.CCGCCGCGCAAGCGCA.A.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCTGA  >  NC_014034/3173467‑3173659
                                                                                                      |                                                                                              
cgcCGGTCACGCCCGCCCCCGCCCCCACCGCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCgcgcaagcgca.ac                                                                                                <  1:4198847/101‑2 (MQ=255)
     gTCCCGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.A.Cgcccc                                                                                          <  2:3302900/101‑1 (MQ=255)
      tcccccccgcccccgccCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGccccc                                                                                          <  2:3233413/96‑1 (MQ=255)
      tCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGccccc                                                                                          <  2:4447773/101‑1 (MQ=255)
      tCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGccccc                                                                                          <  1:1998913/101‑1 (MQ=255)
         cgcccgccCCCGCCCCCACCCCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCccccgcc                                                                                       <  2:3897393/101‑1 (MQ=255)
         cgcccgccCCCGCCCCCACCCCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCccccgcc                                                                                       <  2:4091146/101‑1 (MQ=255)
            ccgcccccgcccCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCccccgccccg                                                                                    >  1:2466598/1‑101 (MQ=255)
             cgcccccgccccCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCgg                                                                                   >  1:3148041/1‑101 (MQ=255)
                 cccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgc                                                                               >  1:3702609/1‑101 (MQ=255)
                  ccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcg                                                                              >  2:170697/1‑101 (MQ=255)
                  ccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcg                                                                              >  2:2861359/1‑101 (MQ=255)
                   cgcnccnCCCCCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcgc                                                                             <  1:693662/101‑1 (MQ=255)
                   cgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcgc                                                                             >  2:1059951/1‑101 (MQ=255)
                     cccccCCCACCCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcgccc                                                                           <  1:2861567/101‑1 (MQ=255)
                       cccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcgcccgc                                                                         >  2:3203897/1‑101 (MQ=255)
                        ccaccaCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcgcccgcg                                                                        <  2:3147817/101‑1 (MQ=255)
                        ccaccaCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGCAAacgcccgcg                                                                        >  2:370479/1‑101 (MQ=255)
                           ccccTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAgcgcccgcgccc                                                                     <  2:2271227/98‑1 (MQ=255)
                            cacTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGcgcccgcgcccg                                                                    >  1:722248/1‑101 (MQ=255)
                             acTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCCAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCCGCCGa                                                                   >  2:81102/1‑101 (MQ=255)
                             acTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGa                                                                   >  2:3519289/1‑101 (MQ=255)
                              cTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGaa                                                                  >  1:3308529/1‑101 (MQ=255)
                              cTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGaa                                                                  >  1:2777402/1‑101 (MQ=255)
                               tCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAg                                                                 >  1:1788786/1‑101 (MQ=255)
                               tCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAg                                                                 >  1:2250503/1‑101 (MQ=255)
                               ccccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAg                                                                 <  2:3647862/100‑1 (MQ=255)
                                cccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAGGCGCCCGCGCCCGAAGc                                                                >  1:1472906/1‑101 (MQ=255)
                                cccGCAGCCCGGAAGCCGCCGCCGGGCGGAGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCACC.GCCCCCGCCCCGGAAGCGCCCGCGCCCGCAGc                                                                >  1:325567/1‑101 (MQ=255)
                                 ccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGcc                                                               >  2:4091318/1‑101 (MQ=255)
                                  cGCAGCCCGGACGCCGCCGCCGGGCGGCGTGCTCTCGCGCCTTTTCG.GCCCGCCGCCCAAGCGCA.ACCCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGccc                                                              <  1:4197055/101‑1 (MQ=255)
                                  cGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGccc                                                              <  2:722168/101‑1 (MQ=255)
                                  cGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGccc                                                              >  1:3639885/1‑101 (MQ=255)
                                  cGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGccc                                                              >  1:782018/1‑101 (MQ=255)
                                   gCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGcccc                                                             >  1:1940887/1‑101 (MQ=255)
                                    cAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCt                                                            >  2:909754/1‑101 (MQ=255)
                                      ccccGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTcc                                                          <  2:1175805/100‑1 (MQ=255)
                                        ccGGAAGCCGCCGCCGGGCGGCGTGCGGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCACC.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCgg                                                        <  2:1472801/101‑1 (MQ=255)
                                          ggAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGcc                                                      >  1:702474/1‑101 (MQ=255)
                                          ggAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGcc                                                      <  1:4088994/101‑1 (MQ=255)
                                           gAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCCGccc                                                     >  2:2693792/1‑101 (MQ=255)
                                            aaGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCg                                                    >  2:1379384/1‑101 (MQ=255)
                                            aaGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCCGCCCg                                                    >  2:3189376/1‑101 (MQ=255)
                                                cgccgccgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAgg                                                >  2:2167304/1‑101 (MQ=255)
                                                 tccgccgGGCGGCGTGCTTTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAggg                                               <  2:1024848/100‑1 (MQ=255)
                                                    gccgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCg                                            <  2:1411550/101‑1 (MQ=255)
                                                      cgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAg                                          >  2:561971/1‑101 (MQ=255)
                                                       gggCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGc                                         >  1:1670171/1‑101 (MQ=255)
                                                         gcgtctttcTGTCCCTCCTTTTCGCC.CCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGccc                                       <  2:3651659/93‑1 (MQ=255)
                                                         gcggcgTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGccc                                       <  2:4317926/101‑1 (MQ=255)
                                                             cgTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                                   >  1:1568678/1‑101 (MQ=255)
                                                              gTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                                  >  2:172950/1‑101 (MQ=255)
                                                              gTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                                  >  2:2144794/1‑101 (MQ=255)
                                                              gTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                                  >  2:2626796/1‑101 (MQ=255)
                                                              gTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGCGCCGAGCCCGGCCt                                  >  1:4407058/1‑101 (MQ=255)
                                                               tGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTg                                 >  1:2203422/1‑101 (MQ=255)
                                                                gCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgc                                >  2:344976/1‑101 (MQ=255)
                                                                 cTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcg                               >  1:3404696/1‑101 (MQ=255)
                                                                  tGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcgc                              >  1:1252541/1‑101 (MQ=255)
                                                                   gTCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGcgcg                             >  2:3997491/1‑101 (MQ=255)
                                                                    tCGCGCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCgg                            >  2:1400277/1‑101 (MQ=255)
                                                                     cgcgCCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCggg                           >  2:4166906/1‑101 (MQ=255)
                                                                        gcCTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggccg                        >  1:3071123/1‑101 (MQ=255)
                                                                         ccTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcgg                       >  1:692339/1‑101 (MQ=255)
                                                                          cTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcggt                      >  1:270049/1‑101 (MQ=255)
                                                                          cTTTTCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGgggcggc                      >  2:2033347/1‑100 (MQ=255)
                                                                            tttCG.GCCCGCCGCGCAAGCGCACC.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt                    <  2:2250358/101‑1 (MQ=255)
                                                                            tttCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt                    <  2:781938/101‑1 (MQ=255)
                                                                             ttCG.GCCCGCCGCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGGCGGGCTg                   >  2:1706526/1‑101 (MQ=255)
                                                                                cggcccgccgCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcg                 >  2:1375862/5‑101 (MQ=255)
                                                                                cgcccggccgcGCAAGCGCA.ACGCCCACGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcg                 <  2:1963762/95‑1 (MQ=255)
                                                                                 ggcccgccgCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcgg                >  2:3215247/4‑101 (MQ=255)
                                                                                   cccgccgCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcggcg              >  1:3096547/2‑101 (MQ=255)
                                                                                   cccgccgCGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGGCGGCCTgcggcg              >  2:2226607/2‑101 (MQ=255)
                                                                                     cgccgcGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTg            >  2:906424/1‑101 (MQ=255)
                                                                                     cgccgcGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCCGCCTGCGCGGGGCGGGCTGCGGCGTg            >  2:89913/1‑101 (MQ=255)
                                                                                       ccgcGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGcc          >  1:1067871/1‑101 (MQ=255)
                                                                                       ccgcGCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGcc          >  2:3321790/1‑101 (MQ=255)
                                                                                        cgcgCAAGCGCA.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGccc         <  2:1788667/101‑1 (MQ=255)
                                                                                         gcgcaagcgca.acGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCg        >  2:2689824/1‑101 (MQ=255)
                                                                                           gcaagcgca.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGc      >  1:4389547/1‑101 (MQ=255)
                                                                                           gcaagcgca.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGc      >  1:2445876/1‑101 (MQ=255)
                                                                                           gcaagcgca.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGc      >  2:2457887/1‑101 (MQ=255)
                                                                                            caagcgca.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGGGTGCCCGGcc     >  2:1750829/1‑101 (MQ=255)
                                                                                            caagcgca.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGcc     >  1:3920809/1‑101 (MQ=255)
                                                                                             aagcgca.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCt    >  1:3892166/1‑101 (MQ=255)
                                                                                             aagcgca.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCt    >  2:2625211/1‑101 (MQ=255)
                                                                                              agcgca.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCTg   >  1:2531872/1‑101 (MQ=255)
                                                                                               gcgca.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCTGa  <  2:2777212/101‑1 (MQ=255)
                                                                                                      |                                                                                              
CGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.G.CCGCCGCGCAAGCGCA.A.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCTGA  >  NC_014034/3173467‑3173659

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG