Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,239,738 +G 1.9% intergenic (+6/+31) lldD → / ← RCAP_rcc03049 L‑lactate dehydrogenase/major facilitator superfamily protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,239,7381.→G 1.9% 4.5 ‑3.3 ‑0.1211intergenic (+6/+31)lldD/RCAP_rcc03049L‑lactate dehydrogenase/major facilitator superfamily protein
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (89/118):  total (93/118)
Fisher's exact test strand distribution p-value = 3.64e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.46e-01

Only 100 of 232 total aligned reads displayed.

ACATCTCGATGGCGCTGTGTGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGAGGCCATCACCAGCGCCACAGCG  >  NC_014034/3239638‑3239838
                                                                                                     |                                                                                                      
 cATCTCGATGGCGCTGTGTGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.g                                                                                                       <  1:3518083/101‑1 (MQ=255)
   tctcGATGGCGCTGTGTGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.ggg                                                                                                     >  2:1440507/1‑101 (MQ=255)
   tctcGATGGCGCTGTGTGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.ggg                                                                                                     <  1:237796/101‑1 (MQ=255)
    ctcGATGGCGCTGTGTGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.gggg                                                                                                    >  1:2097585/1‑100 (MQ=255)
    ctcGAGGGCGCGTTGGGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGTTTC.TCACCACTTACCGCTGAGCAGGC.GGG.cc                                                                                                  <  1:3390044/101‑1 (MQ=255)
     tcGATGGCGCTGTGTGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.ggggc                                                                                                   >  1:128295/1‑99 (MQ=255)
     tcGATGGCGCTGTGTGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.cc                                                                                                  >  2:4444419/1‑101 (MQ=255)
      cGATGGCGCTGTGTGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGATTCTTCACCACTTACCGCTGAGCAGGC.GGG.gcc                                                                                                 >  1:3798114/1‑101 (MQ=255)
        aTGGCGCTGTGTGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.gcccg                                                                                               >  2:1476678/1‑99 (MQ=255)
        aTGGCGCTGTGTGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.gg                                                                                              <  2:2671330/101‑1 (MQ=255)
         tGGCGCTGTGTGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.ggg                                                                                             >  1:3322189/1‑101 (MQ=255)
          ggCGCTGTGTGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.cggg                                                                                            >  2:3227055/1‑100 (MQ=255)
             gcTGTGTGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.gcgcggc                                                                                         >  1:2803314/1‑95 (MQ=255)
               tgtgggGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACAccc                                                                                       <  1:582420/101‑1 (MQ=255)
                 tgtgGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.CGGACAccccc                                                                                     >  2:3912134/1‑100 (MQ=255)
                  gtgGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.GGC.GGGGGAcccccg                                                                                    >  2:4285336/1‑99 (MQ=255)
                  gtgGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.GCC.CGGGCAcccccg                                                                                    >  1:356347/1‑99 (MQ=255)
                  gtgGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGGCAcccccg                                                                                    >  2:2616900/1‑99 (MQ=255)
                   tgGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.CGGGCAccccccg                                                                                   >  1:611817/1‑98 (MQ=255)
                    ggCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGc                                                                                  <  2:4250077/101‑1 (MQ=255)
                     gCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.GCC.CGGGGACCCCCGGcc                                                                                 >  2:3678653/1‑101 (MQ=255)
                      cGAGAAGCGGGTGGAGGATCTCGGCCGCGACGACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACGCCCCGGGCCg                                                                                <  2:4021/101‑1 (MQ=255)
                      cGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGCGGGG.CCCGGGGACCCCCCgccc                                                                                  >  1:3725304/1‑98 (MQ=255)
                      cGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCg                                                                                <  2:3484642/101‑1 (MQ=255)
                        agaAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGGCCCCCCCCGCCggc                                                                              >  2:419595/1‑99 (MQ=255)
                        agaAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTc                                                                              <  2:1083955/101‑1 (MQ=255)
                        agaAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTc                                                                              <  2:3547119/101‑1 (MQ=255)
                         gaAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGCGGGG.CCC.GGGCCCCCCCCGCCCgcc                                                                              >  1:3919560/1‑99 (MQ=255)
                         gaAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGGCCCC.GGGGCCCCCCGGGcgtcc                                                                              >  2:3562938/1‑97 (MQ=255)
                         gaAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCa                                                                             <  2:2203401/101‑1 (MQ=255)
                          aaGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCAt                                                                            <  1:2586669/101‑1 (MQ=255)
                          aaGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.CGGGCACCCCGGGGCGCCac                                                                            >  2:1958249/1‑100 (MQ=255)
                            gCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.CGGGCACCCCCCGCCGGCCTcc                                                                          >  1:503208/1‑101 (MQ=255)
                              gggTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.GCC.GGGGCACCCCCGGcccgcgccccc                                                                        >  1:905776/1‑92 (MQ=255)
                              gggTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.CGGGCACCCCCGGCCgcccccccc                                                                        >  1:2896465/1‑93 (MQ=255)
                                gTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.GCC.GGGGCCACCCCGGCCGCCCCCCCCTc                                                                      >  2:615497/1‑101 (MQ=255)
                                    aGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAggt                                                                  <  2:4175536/101‑2 (MQ=255)
                                    aGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGgc                                                                  <  2:3411647/101‑1 (MQ=255)
                                    aGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGA.CCC.GGGGCCCCCCACGCGGGCATccccccccgg                                                                  >  2:316193/1‑95 (MQ=255)
                                     ggATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGgcg                                                                 <  2:3321957/101‑1 (MQ=255)
                                          tcGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCt                                                            <  2:2909270/101‑1 (MQ=255)
                                          tcGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCt                                                            <  2:544360/101‑1 (MQ=255)
                                           cGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCCCTTACCGCTGAGCAGGC.GGG.CCC.GGGACCCCCCGGGCCGTCATCCCCTCAGGCGCGCCTg                                                           <  2:3598908/101‑1 (MQ=255)
                                           cGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGGCCACCCCCGCCGCCATCCCCCCCGGCGCGcccc                                                           >  1:3301754/1‑99 (MQ=255)
                                           cGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTg                                                           <  2:2085500/101‑1 (MQ=255)
                                             gCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.GCC.GGGGCACCCCGGGCCGGCATACCCCCCGGCGCGcccggc                                                         >  2:2437869/1‑97 (MQ=255)
                                             gCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGAt                                                         <  2:2484279/101‑1 (MQ=255)
                                               cgcgACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATcc                                                       <  1:3877185/101‑1 (MQ=255)
                                               cgcgACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATca                                                       <  1:3053172/101‑2 (MQ=255)
                                                  gACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCt                                                    <  2:503156/101‑1 (MQ=255)
                                                   aCATCCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCtt                                                   <  2:677309/101‑1 (MQ=255)
                                                   aCAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.CGGGCACCCCCGGCCGCCCTCCCCCCCGGCGCGcccggaccgcct                                                   >  1:734430/1‑91 (MQ=255)
                                                    cAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTc                                                  <  1:419635/101‑1 (MQ=255)
                                                      aCCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAg                                                <  1:4172254/101‑1 (MQ=255)
                                                      aCCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAg                                                <  1:3623964/101‑1 (MQ=255)
                                                       ccTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAgc                                               <  1:3146971/101‑1 (MQ=255)
                                                          gctgGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGcc                                            <  1:1913198/101‑1 (MQ=255)
                                                          gctgGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.CGGGCACCCCCGGCCGTCCTCCCCTCCCGCGCGCCCGGTCCGCCTCACCGcc                                            >  2:4039385/1‑101 (MQ=255)
                                                           ctgGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGccc                                           <  1:4024797/101‑1 (MQ=255)
                                                            tgGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGTAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCa                                          >  1:2533552/1‑101 (MQ=255)
                                                            tgGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGGCACCCCCGGCCGGCATCCCCCCCGGCGCGCCCGATCCGCCTCCGCGcccc                                          >  1:247287/1‑100 (MQ=255)
                                                            tgGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCa                                          <  1:1230507/101‑1 (MQ=255)
                                                             ggTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCag                                         <  2:2870762/101‑1 (MQ=255)
                                                              gTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCaga                                        <  2:1685117/101‑1 (MQ=255)
                                                              gTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.CGGGGACCCCCCGCCGGCCTCCCCCCACGCGCGCCCGATCCCCCTCTGCGccccca                                        >  1:3769347/1‑98 (MQ=255)
                                                              gTGCCCGAGGGCTTCTTCACCACTTACCGCGGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCaga                                        <  1:1375869/101‑1 (MQ=255)
                                                               tGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCagag                                       <  1:466831/101‑1 (MQ=255)
                                                                    gAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.GCC.GGGGCACCCCGGGCCGCCATCCCCCCAGGCGCGCCCGATCCGCTTCAGCGCCCCCAGGCCgg                                  >  2:2992276/1‑101 (MQ=255)
                                                                     aGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGCCACCCCCCGCCGCCATCCCCCCAGGCGCGCCCCATCCGCTTCAGCGCCCACACGCCggg                                 >  2:1294938/1‑101 (MQ=255)
                                                                     aGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCggg                                 <  2:3361328/101‑1 (MQ=255)
                                                                      gggCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.CGGGCACCCCGGGGCGTCATCCCCGCCTGCGCGCCCGATCCGCTTCATCGCCCCGACGCCgggg                                >  2:2743561/1‑100 (MQ=255)
                                                                       ggCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCa                               <  2:793507/101‑1 (MQ=255)
                                                                          ttcttcACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGGGACCCCGCGCCGGCCTCCCCCCCGGGGCGCCCGTTCCGCCTCCGCGCCCAAAGGCCGGGCCggg                            >  1:1410075/1‑100 (MQ=255)
                                                                           tcttcACCACTTACCGCTTAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGAt                           <  2:1897217/101‑1 (MQ=255)
                                                                           tcttcACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGAt                           <  2:3894744/101‑1 (MQ=255)
                                                                            cttcACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATc                          <  1:2111473/101‑1 (MQ=255)
                                                                            cttcACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATc                          <  2:2105853/101‑1 (MQ=255)
                                                                            cttcACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATc                          <  2:1268016/101‑1 (MQ=255)
                                                                            cttcACCACTTACCGCTGAGCAGGC.GGG.CCC.CGGGCACCCCCGGCCGGCATCCCCCCAGGCGCGCCCGATCCGCTTCAGCGCCCCGAGGCCCGGGAGGGTc                          >  2:849137/1‑101 (MQ=255)
                                                                             ttcACCACTTACCGCTGAGCAGGC.GGG.CCC.CGGGCACCCCGGGCCCCCCTCCCCCCACGCCCCCCTGATCCCCCTTTGCCCCCCCAGGCCGGGCCGGAGCg                         >  1:1205991/1‑101 (MQ=255)
                                                                              tcACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGGCACCCCCGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCCGAGGCCGGGCAGGGTCGa                        >  2:3737939/1‑101 (MQ=255)
                                                                              tcACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGa                        <  1:4444691/101‑1 (MQ=255)
                                                                                accacTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGAgg                      <  1:3171530/101‑1 (MQ=255)
                                                                                accacTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGAgg                      <  1:4248804/101‑1 (MQ=255)
                                                                                  cacTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGAGGcc                    <  2:4225702/101‑1 (MQ=255)
                                                                                  cacTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGAGGcc                    <  1:4285605/101‑1 (MQ=255)
                                                                                   acTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGAGGCCa                   <  1:1559284/101‑1 (MQ=255)
                                                                                     ttACCGCTGAGCAGGC.GGG.CCC.GGGGCACCCCCGGCCGGCCTCCCCCCCCGCGCGCCCGGTCCGCCTCCGCGCCCCGAGGCCGGGCCGGGGCGAgggcccc                 >  1:107469/1‑96 (MQ=255)
                                                                                     ttACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGAGGCCATc                 <  1:2626443/101‑1 (MQ=255)
                                                                                      tACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGAGGCCATCa                <  2:24789/101‑1 (MQ=255)
                                                                                      tACCGCGGGGCAGTC.GGG.CCC.CGGGCACCGGGCGCCGTCATCCCCTCAGGCGCGCCTGACCGGCTGCAGCACCAAGAGGCCGGGCAGGATCGAGGCCATCa                <  2:3764560/101‑1 (MQ=255)
                                                                                       aCCGCTGAGCAGGC.GGG.CCC.GGGACCCCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGAGGCCATCAc               <  1:1777746/101‑1 (MQ=255)
                                                                                        ccGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGAGGCCATCAcc              <  1:2149971/101‑1 (MQ=255)
                                                                                         cGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGAGGCCATCACCa             <  2:4321413/101‑1 (MQ=255)
                                                                                           cTGAGCAGGC.GGG.GCC.GGGGCACCCCCGGCCGCCCTCCCCTCCGGCGCGCCCGATGCGGATCCGCGCCCAGAGGCCGGGCAGGCTCCCGGGCAACACCaac           >  2:2233700/1‑99 (MQ=255)
                                                                                           cTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGACGCCATCACCAgc           <  1:2423293/101‑1 (MQ=255)
                                                                                             gAGCAGGC.GGG.CCC.GGGGCACCCCCGGCCGGCATCCCCCCCCGCCCGCCTGATCCGCTTCCCCGCCCCGAGGCCCGGCAGGGTCCAGGCCACCAcccccgc         >  2:3093633/1‑96 (MQ=255)
                                                                                                 aGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGAGGCCATCACCAGCGCcaca     >  1:1886807/1‑101 (MQ=255)
                                                                                                 aGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCCCCGGCGCGCCTGATCCGCTTCAGCGCCCCGAGGCCGGGCAGGATCGAGGCCATCACCAGCGCcaca     >  2:3861297/1‑101 (MQ=255)
                                                                                                   gC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGAGGCCATCACCAGCGCCACAGc   <  1:3227284/101‑1 (MQ=255)
                                                                                                     |                                                                                                      
ACATCTCGATGGCGCTGTGTGGCGAGAAGCGGGTGCAGGATCTCGGCCGCGACAACCTGCTGGTGCCCGAGGGCTTCTTCACCACTTACCGCTGAGCAGGC.GGG.CCC.GGGACACCCCGGGCCGTCATCCCCTCAGGCGCGCCTGATCCGCTTCAGCGCCCAGAGGCCGGGCAGGATCGAGGCCATCACCAGCGCCACAGCG  >  NC_014034/3239638‑3239838

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG