Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,171 +C 100% coding (1314/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1711.→C100.0% 461.6 122coding (1314/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (57/65):  ref base (0/0):  total (57/65)

Only 100 of 147 total aligned reads displayed.

GGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCA.GGGCGC.GGCAC.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCT  >  NC_014034/3256072‑3256257
                                                                                                     |                                                                                                 
gggggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAgg                                                                                                    >  2:693890/1‑101 (MQ=255)
  gggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGGGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcg                                                                                                  <  2:2136833/101‑3 (MQ=255)
  gggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcg                                                                                                  >  2:2047431/1‑99 (MQ=255)
  gggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcg                                                                                                  >  1:346480/1‑99 (MQ=255)
  gggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcg                                                                                                  <  2:3256326/101‑3 (MQ=255)
   gggcGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgc                                                                                                 >  1:2551693/4‑98 (MQ=255)
   ggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgc                                                                                                 >  2:2753021/1‑98 (MQ=255)
   ggTCGATCGGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgc                                                                                                 <  2:2217604/101‑4 (MQ=255)
    gTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCCCCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgca                                                                                                <  1:495046/101‑5 (MQ=255)
    gTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgca                                                                                                <  2:1811126/101‑5 (MQ=255)
    gTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgca                                                                                                >  2:1059301/1‑97 (MQ=255)
      cGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagc                                                                                              >  2:3862675/1‑95 (MQ=255)
       gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagct                                                                                             <  2:4412629/101‑8 (MQ=255)
       gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagct                                                                                             <  2:3736919/101‑8 (MQ=255)
         tCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagctgg                                                                                           <  1:3341736/101‑10 (MQ=255)
         tCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagctgg                                                                                           >  1:4114611/1‑92 (MQ=255)
          cTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagctgga                                                                                          >  1:3993783/1‑91 (MQ=255)
          cTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagctgga                                                                                          >  2:2519237/1‑91 (MQ=255)
            gggCGCGGCGGTGCAGCGCGTCGCGGTCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                       <  1:3733523/101‑1 (MQ=255)
            gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                       >  1:3916117/1‑101 (MQ=255)
            gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                       >  1:1958633/1‑101 (MQ=255)
            gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                       >  2:283032/1‑101 (MQ=255)
            gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                       >  2:3900939/1‑101 (MQ=255)
             ggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cc                                                                                      <  1:4087519/101‑1 (MQ=255)
             ggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cc                                                                                      <  2:3863109/101‑1 (MQ=255)
              gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.ccc                                                                                     <  2:825982/101‑2 (MQ=255)
               cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccg                                                                                    >  1:1334638/1‑99 (MQ=255)
                gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgc                                                                                   >  2:294367/1‑98 (MQ=255)
                 cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcg                                                                                  <  2:4122909/101‑5 (MQ=255)
                 cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcg                                                                                  <  1:1670592/101‑5 (MQ=255)
                 cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcg                                                                                  <  2:3763221/101‑5 (MQ=255)
                 cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcg                                                                                  <  1:2418644/101‑5 (MQ=255)
                  ggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcga                                                                                 <  1:566515/101‑6 (MQ=255)
                   gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGAt                                                                                 <  1:1258206/101‑1 (MQ=255)
                    cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATg                                                                                >  2:2068279/1‑101 (MQ=255)
                    cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATg                                                                                <  1:33284/101‑1 (MQ=255)
                     ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATgg                                                                               >  2:606532/1‑101 (MQ=255)
                      gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGTGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcgaaggc                                                                             >  1:2628194/1‑92 (MQ=255)
                      gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggc                                                                              <  1:1439509/101‑2 (MQ=255)
                        gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcac                                                                            <  1:3886669/101‑4 (MQ=255)
                         cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                           >  1:1504409/1‑97 (MQ=255)
                         cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                           <  1:53951/101‑5 (MQ=255)
                          aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacgg                                                                          >  1:3789833/1‑96 (MQ=255)
                          aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacgg                                                                          >  1:258984/1‑96 (MQ=255)
                             gcgTCTCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctg                                                                       <  2:811348/101‑9 (MQ=255)
                              cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                      >  1:4149803/1‑92 (MQ=255)
                               gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                     >  1:4006765/1‑91 (MQ=255)
                                     gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.tt                                                            >  2:3900584/1‑100 (MQ=255)
                                       cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgc                                                          >  2:1242136/1‑98 (MQ=255)
                                        ggggggGTTTTGCTCAAGGGGGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgct                                                         <  1:24564/99‑5 (MQ=255)
                                         aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctc                                                        >  1:3535347/1‑96 (MQ=255)
                                         aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctc                                                        >  1:943869/1‑96 (MQ=255)
                                          ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcg                                                       <  1:4062803/101‑7 (MQ=255)
                                          ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcg                                                       >  2:2226666/1‑95 (MQ=255)
                                          ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.g.ttgctcg                                                       <  2:1740750/101‑7 (MQ=255)
                                           gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.g.ttgctcgc                                                      <  2:1539701/101‑8 (MQ=255)
                                                tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGc                                                  <  1:1802939/101‑1 (MQ=255)
                                                tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGc                                                  <  1:4197041/101‑1 (MQ=255)
                                                 ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCa                                                 <  1:3133041/101‑1 (MQ=255)
                                                 ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCa                                                 <  1:665718/101‑1 (MQ=255)
                                                   gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.gg                                              >  2:1671341/1‑101 (MQ=255)
                                                    cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCC.ggg                                             >  1:1914198/1‑101 (MQ=255)
                                                    cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.ggg                                             >  2:2296240/1‑101 (MQ=255)
                                                      cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCA.GGgcg                                           <  1:2068416/101‑1 (MQ=255)
                                                       aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGgggc                                          >  1:1352026/1‑101 (MQ=255)
                                                         gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gg                                       <  2:2815073/101‑1 (MQ=255)
                                                          ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCC.ggggggggg                                       >  1:1677020/1‑95 (MQ=255)
                                                           gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggc                                     <  2:2356144/101‑3 (MQ=255)
                                                           gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggc                                     <  1:1029373/101‑3 (MQ=255)
                                                           gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggc                                     <  1:283067/101‑3 (MQ=255)
                                                             ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggcac                                   >  1:3908448/1‑97 (MQ=255)
                                                             ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggcac                                   <  1:701771/101‑5 (MQ=255)
                                                                gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCAc.ggg                                >  2:2568168/1‑100 (MQ=255)
                                                                   cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcgg                             <  2:494783/101‑5 (MQ=255)
                                                                   cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcgg                             <  1:4253680/101‑5 (MQ=255)
                                                                    aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcggc                            >  2:1469339/1‑96 (MQ=255)
                                                                     ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCACGGGcg.gcc                           >  2:3599619/1‑101 (MQ=255)
                                                                     ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcc                           <  2:3563113/101‑1 (MQ=255)
                                                                      cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gccc                          <  2:1334541/101‑1 (MQ=255)
                                                                       gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccg                         <  1:1031322/101‑1 (MQ=255)
                                                                       gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccg                         <  2:3148867/101‑1 (MQ=255)
                                                                        cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccgg                        >  1:699642/1‑101 (MQ=255)
                                                                          tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGG.GTTGCTCGTCCGGCAGGGGCCC.GGGACGGGcg.gcccggcc                      >  2:513802/1‑101 (MQ=255)
                                                                          tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGG.TGCTCGCCCGGCC.GGGCGCGGGCACGGGcg.gccccgcc                      >  1:3474846/1‑101 (MQ=255)
                                                                          tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccggcc                      <  2:1100231/101‑1 (MQ=255)
                                                                             ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGAt                   <  1:3446593/101‑1 (MQ=255)
                                                                             ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGAt                   <  2:1438779/101‑1 (MQ=255)
                                                                             ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGAt                   <  1:900555/101‑1 (MQ=255)
                                                                                 gggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCTATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGGTCTGc               >  1:3011321/1‑101 (MQ=255)
                                                                                  ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCg              <  2:554941/101‑1 (MQ=255)
                                                                                     cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCACGGGCG.GCCCGGCCGATCTGCGGcc           >  1:3569720/1‑100 (MQ=255)
                                                                                     cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCt           >  2:2560430/1‑101 (MQ=255)
                                                                                     cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCAC.GGGGCGCCCCGCCGATCttgggcc           >  1:349589/1‑95 (MQ=255)
                                                                                      tGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTTCGGccg          >  1:1872995/1‑99 (MQ=255)
                                                                                       gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTgg         <  1:663097/101‑1 (MQ=255)
                                                                                          cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACg      <  2:4114351/97‑1 (MQ=255)
                                                                                          cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCCGCCGCTCTGCGGCTGGagg      >  2:2544728/5‑99 (MQ=255)
                                                                                            tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGCTCTGCGGCTGGGCggg    >  1:1833705/3‑101 (MQ=255)
                                                                                            tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCCGGGCggg    >  1:3211977/3‑101 (MQ=255)
                                                                                              gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCt  <  2:3789580/101‑1 (MQ=255)
                                                                                                     |                                                                                                 
GGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCA.GGGCGC.GGCAC.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCT  >  NC_014034/3256072‑3256257

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG