Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,180 +C 100% coding (1323/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1801.→C100.0% 459.5 121coding (1323/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (58/63):  ref base (0/0):  total (58/63)

Only 100 of 141 total aligned reads displayed.

GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCA.GGGCGC.GGCAC.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGC  >  NC_014034/3256084‑3256270
                                                                                                    |                                                                                                  
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                                   >  1:3916117/1‑101 (MQ=255)
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                                   >  2:283032/1‑101 (MQ=255)
  gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.ccc                                                                                                 <  2:825982/101‑2 (MQ=255)
   cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccg                                                                                                <  2:3955621/101‑3 (MQ=255)
    gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgc                                                                                               >  2:294367/1‑98 (MQ=255)
     cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcg                                                                                              <  2:4122909/101‑5 (MQ=255)
     cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcg                                                                                              <  1:2418644/101‑5 (MQ=255)
     cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcg                                                                                              <  2:3763221/101‑5 (MQ=255)
      ggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcga                                                                                             <  1:566515/101‑6 (MQ=255)
       gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGAt                                                                                             <  1:1258206/101‑1 (MQ=255)
       gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGAt                                                                                             >  2:2508695/1‑101 (MQ=255)
        cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATg                                                                                            >  2:2068279/1‑101 (MQ=255)
         ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATgg                                                                                           >  2:606532/1‑101 (MQ=255)
         ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATgg                                                                                           <  2:389661/101‑1 (MQ=255)
          gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGTGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcgaaggc                                                                                         >  1:2628194/1‑92 (MQ=255)
            gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcac                                                                                        <  1:3886669/101‑4 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                                       <  1:191685/101‑5 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                                       >  1:1504409/1‑97 (MQ=255)
              aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacgg                                                                                      >  1:3789833/1‑96 (MQ=255)
                 gcgTCTCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctg                                                                                   <  2:811348/101‑9 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                                  >  1:4149803/1‑92 (MQ=255)
                   gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                                 >  1:3610445/1‑91 (MQ=255)
                         gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.tt                                                                         >  2:3900584/1‑100 (MQ=255)
                           cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgc                                                                       >  2:1242136/1‑98 (MQ=255)
                              ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcg                                                                    >  2:2226666/1‑95 (MQ=255)
                              ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcg                                                                    <  1:4062803/101‑7 (MQ=255)
                              ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcc                                                                    >  1:3422125/1‑95 (MQ=255)
                              ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAg.ttgctcg                                                                    <  2:1740750/101‑7 (MQ=255)
                               gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAg.ttgctcgc                                                                   <  2:1539701/101‑8 (MQ=255)
                                ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcgcc                                                                  <  1:2586236/101‑9 (MQ=255)
                                ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAg.ttgctcgcc                                                                  <  2:2228428/101‑9 (MQ=255)
                                  cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgcnngnccn                                                                >  1:1520125/1‑91 (MQ=255)
                                   ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCgg                                                                >  1:4297851/1‑101 (MQ=255)
                                    tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGc                                                               <  1:1802939/101‑1 (MQ=255)
                                    tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGc                                                               <  1:4197041/101‑1 (MQ=255)
                                     ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCa                                                              <  2:59330/101‑1 (MQ=255)
                                     ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCa                                                              <  1:665718/101‑1 (MQ=255)
                                     ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCa                                                              <  1:3133041/101‑1 (MQ=255)
                                      tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.g                                                            >  1:772153/1‑101 (MQ=255)
                                       gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.gg                                                           >  2:1671341/1‑101 (MQ=255)
                                        cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCC.ggg                                                          >  1:1914198/1‑101 (MQ=255)
                                        cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.ggg                                                          >  1:3952799/1‑101 (MQ=255)
                                        cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.ggg                                                          >  2:2296240/1‑101 (MQ=255)
                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGgc                                                         >  2:1037877/1‑101 (MQ=255)
                                          cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCA.GGgcg                                                        <  1:2068416/101‑1 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGgggc                                                       >  1:1352026/1‑101 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGgcgc                                                       <  1:4013088/101‑1 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGgcgc                                                       <  2:3821496/101‑1 (MQ=255)
                                             gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGGGC.gg                                                    >  2:495393/1‑101 (MQ=255)
                                             gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGC.gg                                                    <  2:2815073/101‑1 (MQ=255)
                                              ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCC.ggggggggg                                                    >  1:1677020/1‑95 (MQ=255)
                                               gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGC.gggc                                                  <  1:1029373/101‑3 (MQ=255)
                                               gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGC.gggc                                                  <  1:283067/101‑3 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGC.gggcac                                                >  1:3908448/1‑97 (MQ=255)
                                                   cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.gg                                              >  1:3470692/1‑101 (MQ=255)
                                                    gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.ggg                                             >  2:2568168/1‑100 (MQ=255)
                                                       cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcgg                                          <  2:494783/101‑5 (MQ=255)
                                                       cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcgg                                          <  1:558534/101‑5 (MQ=255)
                                                       cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcgg                                          <  1:4253680/101‑5 (MQ=255)
                                                        aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcggc                                         >  2:1469339/1‑96 (MQ=255)
                                                         ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGGGCGGGCACGGGcg.gcc                                        >  2:3599619/1‑101 (MQ=255)
                                                         ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcc                                        <  1:1834940/101‑1 (MQ=255)
                                                         ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcc                                        <  2:3563113/101‑1 (MQ=255)
                                                          cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gccc                                       <  2:1334541/101‑1 (MQ=255)
                                                           gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcggtgta                                      <  2:4149542/101‑9 (MQ=255)
                                                           gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccg                                      <  2:3148867/101‑1 (MQ=255)
                                                           gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccg                                      <  1:1031322/101‑1 (MQ=255)
                                                            cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccgg                                     >  1:699642/1‑101 (MQ=255)
                                                             aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccggc                                    <  1:1403647/101‑1 (MQ=255)
                                                              tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGGGTTGCTCGTCCGGCAGGGGCCC.GGGACGGGcg.gcccggcc                                   >  2:513802/1‑101 (MQ=255)
                                                              tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccggcc                                   <  2:1100231/101‑1 (MQ=255)
                                                               ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCg                                  <  1:171620/101‑1 (MQ=255)
                                                                 ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGAt                                <  1:900555/101‑1 (MQ=255)
                                                                 ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGAt                                <  2:1438779/101‑1 (MQ=255)
                                                                 ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGAt                                <  1:3446593/101‑1 (MQ=255)
                                                                     gggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCTATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGGTCTGc                            >  1:3011321/1‑101 (MQ=255)
                                                                      ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGGGCGGGCACGGGCG.GCCCGGCCGATCTGCg                           >  1:4098301/1‑101 (MQ=255)
                                                                      ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCCCGGGCG.GGCCGGCCGCTCTGCg                           >  2:2074016/1‑101 (MQ=255)
                                                                         cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGGGCGGGCACGGGCG.GCCCGGCCGATCTGCGGcc                        >  1:3569720/1‑100 (MQ=255)
                                                                         cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCt                        >  1:663391/1‑101 (MQ=255)
                                                                         cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCt                        >  2:2560430/1‑101 (MQ=255)
                                                                         cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAC.GGGGCGCCCCGCCGATCttgggcc                        >  1:349589/1‑95 (MQ=255)
                                                                          tGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGGG.GCCCCGGCGGTCTGCGGCTg                       >  2:3261672/1‑101 (MQ=255)
                                                                              cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCCGCCGCTCTGCGGCTGGagg                   >  2:2544728/5‑99 (MQ=255)
                                                                               ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCCGCCGATCTGCGGCTgggggg                  >  1:3528768/4‑97 (MQ=255)
                                                                                tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGCTCTGCGGCTGGGCggg                 >  1:1833705/3‑101 (MQ=255)
                                                                                tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCCGGGCggg                 >  1:3211977/3‑101 (MQ=255)
                                                                                  gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCt               <  2:3789580/101‑1 (MQ=255)
                                                                                      agttcaagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGGTCTGCGGCTGGGCCGGGTCCTg           >  2:292580/10‑101 (MQ=255)
                                                                                       ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa          <  1:1469444/93‑1 (MQ=255)
                                                                                       ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa          <  1:254437/93‑1 (MQ=255)
                                                                                       ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa          <  1:2047568/93‑1 (MQ=255)
                                                                                         cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg        <  2:3908194/95‑1 (MQ=255)
                                                                                          gcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGc       >  1:3622534/6‑101 (MQ=255)
                                                                                           cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGcc      >  2:2762653/5‑101 (MQ=255)
                                                                                           cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc      <  1:1962583/97‑1 (MQ=255)
                                                                                             gctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGGGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGGTGCCgg    >  1:2372415/3‑101 (MQ=255)
                                                                                             gctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCgg    <  2:2548462/99‑1 (MQ=255)
                                                                                              ctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg   <  2:3915863/100‑1 (MQ=255)
                                                                                               tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGGG.GCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGATGCCgggg  >  1:946604/1‑100 (MQ=255)
                                                                                                    |                                                                                                  
GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCA.GGGCGC.GGCAC.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGC  >  NC_014034/3256084‑3256270

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG