Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,189 +C 93.9% coding (1332/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1891.→C93.9% 14.9 ‑2.1 0.0115coding (1332/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (51/57):  ref base (1/6):  total (52/63)
Fisher's exact test strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCA.GGGCGC.GGCAC.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256093‑3256278
                                                                                                     |                                                                                                
ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATgg                                                                                                   >  2:606532/1‑101 (MQ=255)
ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATgg                                                                                                   <  2:389661/101‑1 (MQ=255)
    cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                                               >  1:1504409/1‑97 (MQ=255)
    cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                                               <  1:53951/101‑5 (MQ=255)
    cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                                               <  1:191685/101‑5 (MQ=255)
     aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacgg                                                                                              >  1:3789833/1‑96 (MQ=255)
     aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacgg                                                                                              >  1:258984/1‑96 (MQ=255)
     aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacgg                                                                                              >  2:3005961/1‑96 (MQ=255)
        gcgTCTCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctg                                                                                           <  2:811348/101‑9 (MQ=255)
          gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                                         >  1:3610445/1‑91 (MQ=255)
                gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.tt                                                                                 >  2:3900584/1‑100 (MQ=255)
                  cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgc                                                                               >  2:1242136/1‑98 (MQ=255)
                   ggggggGTTTTGCTCAAGGGGGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgct                                                                              <  1:24564/99‑5 (MQ=255)
                    aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctc                                                                             >  1:943869/1‑96 (MQ=255)
                    aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctc                                                                             >  1:3535347/1‑96 (MQ=255)
                     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcg                                                                            <  2:1219197/101‑7 (MQ=255)
                     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcg                                                                            >  1:3123774/1‑95 (MQ=255)
                     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcg                                                                            <  1:4062803/101‑7 (MQ=255)
                     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcg                                                                            >  2:2226666/1‑95 (MQ=255)
                     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcc                                                                            >  1:3422125/1‑95 (MQ=255)
                     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAg.ttgctcg                                                                            <  2:1740750/101‑7 (MQ=255)
                      gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAg.ttgctcgc                                                                           <  2:1539701/101‑8 (MQ=255)
                       ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgctcgcc                                                                          <  1:2586236/101‑9 (MQ=255)
                       ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAg.ttgctcgcc                                                                          <  2:2228428/101‑9 (MQ=255)
                         cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAg.ttgcnngnccn                                                                        >  1:1520125/1‑91 (MQ=255)
                           tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGc                                                                       <  1:1802939/101‑1 (MQ=255)
                           tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGc                                                                       <  2:3632108/101‑1 (MQ=255)
                           tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGc                                                                       <  1:4197041/101‑1 (MQ=255)
                            ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCa                                                                      <  1:3133041/101‑1 (MQ=255)
                            ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCa                                                                      <  1:665718/101‑1 (MQ=255)
                              gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.gg                                                                   >  2:1671341/1‑101 (MQ=255)
                               cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCC.ggg                                                                  >  1:1914198/1‑101 (MQ=255)
                               cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.ggg                                                                  >  1:3952799/1‑101 (MQ=255)
                               cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.ggg                                                                  <  2:2585952/101‑1 (MQ=255)
                                tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGgc                                                                 <  1:4161350/101‑1 (MQ=255)
                                  aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGgggc                                                               >  1:1352026/1‑101 (MQ=255)
                                  aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGgcgc                                                               <  1:4013088/101‑1 (MQ=255)
                                    gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGGGC.gg                                                            >  2:495393/1‑101 (MQ=255)
                                    gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGC.gg                                                            <  2:1431411/101‑1 (MQ=255)
                                     ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCC.ggggggggg                                                            >  1:1677020/1‑95 (MQ=255)
                                      gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGC.gggc                                                          <  2:2356144/101‑3 (MQ=255)
                                      gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGC.gggc                                                          <  1:1029373/101‑3 (MQ=255)
                                        ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGC.gggcac                                                        >  1:3908448/1‑97 (MQ=255)
                                        ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGC.gggcac                                                        <  1:701771/101‑5 (MQ=255)
                                          cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.gg                                                      >  1:3470692/1‑101 (MQ=255)
                                           gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.ggg                                                     >  2:2568168/1‑100 (MQ=255)
                                            ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggc                                                    <  2:2548104/101‑3 (MQ=255)
                                              gaCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcgg                                                  <  2:742220/100‑5 (MQ=255)
                                              cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcgg                                                  <  1:4253680/101‑5 (MQ=255)
                                              cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcgg                                                  <  1:558534/101‑5 (MQ=255)
                                               aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcggc                                                 >  2:1469339/1‑96 (MQ=255)
                                                ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGGGCGGGCACGGGcg.gcc                                                >  2:3599619/1‑101 (MQ=255)
                                                ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcc                                                <  1:1834940/101‑1 (MQ=255)
                                                 cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gccc                                               <  2:1334541/101‑1 (MQ=255)
                                                  gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAc.gggcggtgta                                              <  2:4149542/101‑9 (MQ=255)
                                                  gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccg                                              <  1:1031322/101‑1 (MQ=255)
                                                   cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccgg                                             >  1:699642/1‑101 (MQ=255)
                                                    aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccggc                                            <  1:1403647/101‑1 (MQ=255)
                                                     tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGGGTTGCTCGTCCGGCAGGGGCCC.GGGACGGGcg.gcccggcc                                           >  2:513802/1‑101 (MQ=255)
                                                     tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGcg.gcccggcc                                           <  2:1100231/101‑1 (MQ=255)
                                                      ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCg                                          <  1:171620/101‑1 (MQ=255)
                                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGAt                                        <  2:1438779/101‑1 (MQ=255)
                                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGAt                                        <  1:3446593/101‑1 (MQ=255)
                                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGAt                                        <  1:900555/101‑1 (MQ=255)
                                                         cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATc                                       <  2:1958504/101‑1 (MQ=255)
                                                            gggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCTATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGGTCTGc                                    >  1:3011321/1‑101 (MQ=255)
                                                             ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGGGCGGGCACGGGCG.GCCCGGCCGATCTGCg                                   >  1:4098301/1‑101 (MQ=255)
                                                             ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCCCGGGCG.GGCCGGCCGCTCTGCg                                   >  2:2074016/1‑101 (MQ=255)
                                                             ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCg                                   <  2:554941/101‑1 (MQ=255)
                                                                cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCAC.GGGGCGCCCCGCCGATCttgggcc                                >  1:349589/1‑95 (MQ=255)
                                                                 tGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGGG.GCCCCGGCGGTCTGCGGCTg                               >  2:3261672/1‑101 (MQ=255)
                                                                  gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTgg                              <  1:663097/101‑1 (MQ=255)
                                                                     cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACg                           <  2:4114351/97‑1 (MQ=255)
                                                                     cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCCGCCGCTCTGCGGCTGGagg                           >  2:2544728/5‑99 (MQ=255)
                                                                      ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACgg                          <  1:3901193/98‑1 (MQ=255)
                                                                      ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCCGCCGATCTGCGGCTgggggg                          >  1:3528768/4‑97 (MQ=255)
                                                                       tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGCTCTGCGGCTGGGCggg                         >  1:1833705/3‑101 (MQ=255)
                                                                         gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCt                       <  2:3789580/101‑1 (MQ=255)
                                                                             agttcaagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGGTCTGCGGCTGGGCCGGGTCCTg                   >  2:292580/10‑101 (MQ=255)
                                                                              ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                  <  1:1469444/93‑1 (MQ=255)
                                                                              ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                  <  1:2047568/93‑1 (MQ=255)
                                                                                cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                <  2:3908194/95‑1 (MQ=255)
                                                                                 gcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGc               >  1:3622534/6‑101 (MQ=255)
                                                                                 gcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc               <  2:4160713/96‑1 (MQ=255)
                                                                                 gcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc               <  2:3474606/96‑1 (MQ=255)
                                                                                  cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGGGCCTGTTGcc              >  2:215707/5‑101 (MQ=255)
                                                                                  cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGcc              >  2:2762653/5‑101 (MQ=255)
                                                                                  cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGcc              >  1:2369759/5‑101 (MQ=255)
                                                                                  cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc              <  1:1962583/97‑1 (MQ=255)
                                                                                    gctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGGGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGGTGCCgg            >  1:2372415/3‑101 (MQ=255)
                                                                                    gctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCgg            <  2:2548462/99‑1 (MQ=255)
                                                                                      tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGGG.GCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGATGCCgggg          >  1:946604/1‑100 (MQ=255)
                                                                                      tGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGGG.GCCCGGCCGATCTGCGGCTGGGCGGGCCCCCGATGCCgggg          >  1:1750420/1‑100 (MQ=255)
                                                                                         aacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc       <  1:2744790/98‑1 (MQ=255)
                                                                                         aacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCCGGGCGGGCTCCTGATGCCGGGcgcc       >  2:2089296/4‑101 (MQ=255)
                                                                                         aacccGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGGG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGcccc       >  2:2892527/4‑101 (MQ=255)
                                                                                            ccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg    <  2:3313452/101‑1 (MQ=255)
                                                                                              gcgATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGGGCCCGATGCCGGGCGCcgcggc  >  1:4151829/1‑99 (MQ=255)
                                                                                              gcgATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCGGGag  >  2:3691921/1‑100 (MQ=255)
                                                                                              gcgATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA.GGGCGCGGGCACGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc  <  1:3273288/101‑1 (MQ=255)
                                                                                                     |                                                                                                
GGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCA.GGGCGC.GGCAC.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256093‑3256278

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG