Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,220 +G 100% coding (1363/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2201.→G100.0% 387.1 101coding (1363/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (41/60):  ref base (0/0):  total (41/60)

Only 100 of 136 total aligned reads displayed.

AAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCA.GGGCGC.GGCA.C.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCC  >  NC_014034/3256127‑3256314
                                                                                                        |                                                                                                 
aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGgggc                                                                                                    >  1:1352026/1‑101 (MQ=255)
aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGgcgc                                                                                                    <  2:3821496/101‑1 (MQ=255)
aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGgcgc                                                                                                    <  1:4013088/101‑1 (MQ=255)
  gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGC.gg                                                                                                 >  2:495393/1‑101 (MQ=255)
  gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gg                                                                                                 <  2:2815073/101‑1 (MQ=255)
    gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggc                                                                                               <  1:1029373/101‑3 (MQ=255)
    gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggc                                                                                               <  2:2356144/101‑3 (MQ=255)
    gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGC.gggc                                                                                               <  1:283067/101‑3 (MQ=255)
            gaCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggcgg                                                                                      <  2:742220/100‑5 (MQ=255)
            cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggcgg                                                                                      <  1:4253680/101‑5 (MQ=255)
             aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggcggc                                                                                     >  2:1469339/1‑96 (MQ=255)
              ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCA.CGGGcg.gcc                                                                                    >  2:3599619/1‑101 (MQ=255)
              ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcc                                                                                    <  2:3563113/101‑1 (MQ=255)
               cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gccc                                                                                   <  2:1334541/101‑1 (MQ=255)
                gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggcggtgta                                                                                  <  2:4149542/101‑9 (MQ=255)
                gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcccg                                                                                  <  1:1031322/101‑1 (MQ=255)
                gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcccg                                                                                  <  2:3148867/101‑1 (MQ=255)
                 cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcccgg                                                                                 >  1:699642/1‑101 (MQ=255)
                   tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGG.GTTGCTCGTCCGGCAGGGGCCC.GGGA.CGGGcg.gcccggcc                                                                               >  2:513802/1‑101 (MQ=255)
                   tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGG.TGCTCGCCCGGCC.GGGCGCGGGCA.CGGGcg.gccccgcc                                                                               >  1:3474846/1‑101 (MQ=255)
                   tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcccggcc                                                                               <  2:1100231/101‑1 (MQ=255)
                      ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGAt                                                                            <  1:900555/101‑1 (MQ=255)
                      ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGAt                                                                            <  2:1438779/101‑1 (MQ=255)
                      ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGAt                                                                            <  1:3446593/101‑1 (MQ=255)
                       cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATc                                                                           <  2:1958504/101‑1 (MQ=255)
                          gggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCTATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGGTCTGc                                                                        >  1:3011321/1‑101 (MQ=255)
                           ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCA.CGGGCG.GCCCGGCCGATCTGCg                                                                       >  1:4098301/1‑101 (MQ=255)
                           ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCC.CGGGCG.GGCCGGCCGCTCTGCg                                                                       >  2:2074016/1‑101 (MQ=255)
                              cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCt                                                                    >  1:663391/1‑101 (MQ=255)
                              cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCt                                                                    >  2:2560430/1‑101 (MQ=255)
                              cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.C.GGGGCGCCCCGCCGATCttgggcc                                                                    >  1:349589/1‑95 (MQ=255)
                               tGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTTCGGccg                                                                   >  1:1872995/1‑99 (MQ=255)
                                gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTgg                                                                  <  1:663097/101‑1 (MQ=255)
                                   cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACg                                                               <  2:4114351/97‑1 (MQ=255)
                                   cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCCGCCGCTCTGCGGCTGGagg                                                               >  2:2544728/5‑99 (MQ=255)
                                    ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACgg                                                              <  1:3901193/98‑1 (MQ=255)
                                    ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCCGCCGATCTGCGGCTgggggg                                                              >  1:3528768/4‑97 (MQ=255)
                                     tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGCTCTGCGGCTGGGCggg                                                             >  1:1833705/3‑101 (MQ=255)
                                     tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCCGGGCggg                                                             >  1:3211977/3‑101 (MQ=255)
                                            ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                                      <  1:1469444/93‑1 (MQ=255)
                                            ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                                      <  1:254437/93‑1 (MQ=255)
                                               gcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGc                                                   >  1:3622534/6‑101 (MQ=255)
                                               gcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc                                                   <  2:3474606/96‑1 (MQ=255)
                                               gcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc                                                   <  2:4160713/96‑1 (MQ=255)
                                                cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGGGCCTGTTGcc                                                  >  2:215707/5‑101 (MQ=255)
                                                cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGcc                                                  >  1:2369759/5‑101 (MQ=255)
                                                cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                                                  <  1:1962583/97‑1 (MQ=255)
                                                  gctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGGTGCCgg                                                >  1:2372415/3‑101 (MQ=255)
                                                  gctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCgg                                                <  2:2548462/99‑1 (MQ=255)
                                                   ctgtaccccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCAATCTGCGGCTGGACGGGCTCCTGATGCCggg                                               <  1:1647977/94‑1 (MQ=255)
                                                   ctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg                                               <  2:3915863/100‑1 (MQ=255)
                                                    tGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGGG.GCCCGGCCGATCTGCGGCTGGGCGGGCCCCCGATGCCgggg                                              >  1:1750420/1‑100 (MQ=255)
                                                       aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                                           <  1:2744790/98‑1 (MQ=255)
                                                       aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                                           <  2:2137209/98‑1 (MQ=255)
                                                       aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                                           <  2:1823918/98‑1 (MQ=255)
                                                       aacccGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGGG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGcccc                                           >  2:2892527/4‑101 (MQ=255)
                                                        acccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg                                          <  2:1359245/99‑1 (MQ=255)
                                                          ccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                                        <  2:3313452/101‑1 (MQ=255)
                                                           cgcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGCTCTGCGGCTGGGCGGGCTCCCGGTGCCGGGCGCcgcgc                                       >  1:1755174/1‑100 (MQ=255)
                                                            gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCGGGag                                      >  2:3691921/1‑100 (MQ=255)
                                                            gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                                      <  1:3273288/101‑1 (MQ=255)
                                                                  ggcacggctggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCATG.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCTCGACGCCcg                                 <  2:1073871/91‑1 (MQ=255)
                                                                      cggctggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCt                             <  2:3123550/95‑1 (MQ=255)
                                                                          tggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc                         >  1:126989/3‑101 (MQ=255)
                                                                          tggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc                         <  2:2593186/99‑1 (MQ=255)
                                                                           ggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg                        >  2:389409/2‑101 (MQ=255)
                                                                           ggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg                        >  1:1326670/2‑101 (MQ=255)
                                                                              ccgagttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCCAg                      >  2:1315414/10‑101 (MQ=255)
                                                                               cgagttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAgg                     <  1:3599867/93‑1 (MQ=255)
                                                                               cgagttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCCCGCTGATCGAgg                     >  1:4073944/9‑101 (MQ=255)
                                                                               cgagttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATACCGGGCGCCGCGACGCCCGCGCTGAACGAgg                     >  1:2108039/9‑101 (MQ=255)
                                                                                gagttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGa                    >  2:4371108/8‑101 (MQ=255)
                                                                                gagttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGa                    >  2:2208765/8‑101 (MQ=255)
                                                                                 ggttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAt                   <  1:90616/95‑1 (MQ=255)
                                                                                 agttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCGCGACGCCCCCGCTGATCGAGGAt                   >  2:4089098/7‑101 (MQ=255)
                                                                                 agttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAt                   >  1:810548/7‑101 (MQ=255)
                                                                                  gttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtc                  <  2:1127743/96‑1 (MQ=255)
                                                                                   ttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGGGCCGCGACGCCCGCGCTGATCGAGGAtct                 >  1:2794287/5‑101 (MQ=255)
                                                                                    tgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtctc                <  1:4261277/98‑1 (MQ=255)
                                                                                     gctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCg               <  2:1404421/99‑1 (MQ=255)
                                                                                       tCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAc             >  2:2930903/1‑101 (MQ=255)
                                                                                       tCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAc             <  2:258957/101‑1 (MQ=255)
                                                                                       tCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAc             >  2:4336664/1‑101 (MQ=255)
                                                                                        cGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGGTGATCGAGGAGCTCGAcc            >  2:2553826/1‑101 (MQ=255)
                                                                                        cGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAcc            <  2:518091/101‑1 (MQ=255)
                                                                                        cGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAcc            <  2:1676909/101‑1 (MQ=255)
                                                                                        cGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAcc            <  1:1648406/101‑1 (MQ=255)
                                                                                         gCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcg           <  1:2074153/101‑1 (MQ=255)
                                                                                         gCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcg           >  2:2884350/1‑101 (MQ=255)
                                                                                           ccGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGTCGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcgcg         >  2:2993774/1‑101 (MQ=255)
                                                                                           ccGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcgcg         <  2:3185292/101‑1 (MQ=255)
                                                                                             ggCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGcc       >  2:3799793/1‑101 (MQ=255)
                                                                                             ggCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGcc       <  2:349554/101‑1 (MQ=255)
                                                                                             ggCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGcc       <  1:294402/101‑1 (MQ=255)
                                                                                              gCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCg      >  2:2602528/1‑101 (MQ=255)
                                                                                               cA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCgg     >  2:1092771/1‑101 (MQ=255)
                                                                                                a.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGc    >  2:4341316/1‑101 (MQ=255)
                                                                                                a.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGc    >  1:1135231/1‑101 (MQ=255)
                                                                                                  gggCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGcc   >  1:2063906/1‑101 (MQ=255)
                                                                                                   ggCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGccc  >  2:630188/1‑101 (MQ=255)
                                                                                                        |                                                                                                 
AAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCA.GGGCGC.GGCA.C.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCC  >  NC_014034/3256127‑3256314

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG