Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,225 +G 100% coding (1368/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2251.→G100.0% 346.7 93coding (1368/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (36/57):  ref base (0/0):  total (36/57)

Only 100 of 127 total aligned reads displayed.

CGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCA.GGGCGC.GGCA.C.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCCCTA  >  NC_014034/3256135‑3256317
                                                                                                       |                                                                                             
cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gg                                                                                             >  1:3470692/1‑101 (MQ=255)
  ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggc                                                                                           <  2:2548104/101‑3 (MQ=255)
    gaCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggcgg                                                                                         <  2:742220/100‑5 (MQ=255)
    cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggcgg                                                                                         <  2:494783/101‑5 (MQ=255)
    cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggcgg                                                                                         <  1:558534/101‑5 (MQ=255)
     aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggcggc                                                                                        >  2:1469339/1‑96 (MQ=255)
      ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCA.CGGGcg.gcc                                                                                       >  2:3599619/1‑101 (MQ=255)
      ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcc                                                                                       <  1:1834940/101‑1 (MQ=255)
       cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gccc                                                                                      <  2:1334541/101‑1 (MQ=255)
        gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.c.gggcggtgta                                                                                     <  2:4149542/101‑9 (MQ=255)
        gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcccg                                                                                     <  2:3148867/101‑1 (MQ=255)
        gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcccg                                                                                     <  1:1031322/101‑1 (MQ=255)
         cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcccgg                                                                                    >  1:699642/1‑101 (MQ=255)
          aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcccggc                                                                                   <  1:1403647/101‑1 (MQ=255)
           tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGG.GTTGCTCGTCCGGCAGGGGCCC.GGGA.CGGGcg.gcccggcc                                                                                  >  2:513802/1‑101 (MQ=255)
           tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGG.TGCTCGCCCGGCC.GGGCGCGGGCA.CGGGcg.gccccgcc                                                                                  >  1:3474846/1‑101 (MQ=255)
           tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGcg.gcccggcc                                                                                  <  2:1100231/101‑1 (MQ=255)
              ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGAt                                                                               <  1:3446593/101‑1 (MQ=255)
              ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGAt                                                                               <  1:900555/101‑1 (MQ=255)
              ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGAt                                                                               <  2:1438779/101‑1 (MQ=255)
               cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATc                                                                              <  2:1958504/101‑1 (MQ=255)
                  gggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCTATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGGTCTGc                                                                           >  1:3011321/1‑101 (MQ=255)
                   ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCA.CGGGCG.GCCCGGCCGATCTGCg                                                                          >  1:4098301/1‑101 (MQ=255)
                   ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCC.CGGGCG.GGCCGGCCGCTCTGCg                                                                          >  2:2074016/1‑101 (MQ=255)
                   ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCg                                                                          <  2:554941/101‑1 (MQ=255)
                      cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGcc                                                                       >  1:3569720/1‑100 (MQ=255)
                      cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCt                                                                       >  2:2560430/1‑101 (MQ=255)
                      cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.C.GGGGCGCCCCGCCGATCttgggcc                                                                       >  1:349589/1‑95 (MQ=255)
                        gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTgg                                                                     <  1:663097/101‑1 (MQ=255)
                           cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACg                                                                  <  2:4114351/97‑1 (MQ=255)
                            ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACgg                                                                 <  1:3901193/98‑1 (MQ=255)
                             tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGCTCTGCGGCTGGGCggg                                                                >  1:1833705/3‑101 (MQ=255)
                             tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCCGGGCggg                                                                >  1:3211977/3‑101 (MQ=255)
                               gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCt                                                              <  2:3789580/101‑1 (MQ=255)
                                   agttcaagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGGTCTGCGGCTGGGCCGGGTCCTg                                                          >  2:292580/10‑101 (MQ=255)
                                    ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                                         <  1:1469444/93‑1 (MQ=255)
                                    ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                                         <  1:2047568/93‑1 (MQ=255)
                                      cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                                                       <  2:3908194/95‑1 (MQ=255)
                                       gcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGc                                                      >  1:3622534/6‑101 (MQ=255)
                                       gcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc                                                      <  2:4160713/96‑1 (MQ=255)
                                       gcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc                                                      <  2:3474606/96‑1 (MQ=255)
                                        cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGGGCCTGTTGcc                                                     >  2:215707/5‑101 (MQ=255)
                                        cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGcc                                                     >  2:2762653/5‑101 (MQ=255)
                                        cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGcc                                                     >  1:2369759/5‑101 (MQ=255)
                                        cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                                                     <  1:1962583/97‑1 (MQ=255)
                                          gctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGGGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGGTGCCgg                                                   >  1:2372415/3‑101 (MQ=255)
                                          gctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCgg                                                   <  2:2548462/99‑1 (MQ=255)
                                           ctgtaccccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCAATCTGCGGCTGGACGGGCTCCTGATGCCggg                                                  <  1:1647977/94‑1 (MQ=255)
                                           ctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg                                                  <  2:3915863/100‑1 (MQ=255)
                                            tGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGGG.GCCCGGCCGATCTGCGGCTGGGCGGGCCCCCGATGCCgggg                                                 >  1:1750420/1‑100 (MQ=255)
                                               aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                                              <  1:2744790/98‑1 (MQ=255)
                                               aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                                              <  2:1823918/98‑1 (MQ=255)
                                               aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCCGGGCGGGCTCCTGATGCCGGGcgcc                                              >  2:2089296/4‑101 (MQ=255)
                                               aacccGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGGG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGcccc                                              >  2:2892527/4‑101 (MQ=255)
                                                  ccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                                           <  2:3313452/101‑1 (MQ=255)
                                                   cgcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGCTCTGCGGCTGGGCGGGCTCCCGGTGCCGGGCGCcgcgc                                          >  1:1755174/1‑100 (MQ=255)
                                                    gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGGGCCCGATGCCGGGCGCcgcggc                                         >  1:4151829/1‑99 (MQ=255)
                                                    gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCGGGag                                         >  2:3691921/1‑100 (MQ=255)
                                                          ggcacggctggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCATG.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCTCGACGCCcg                                    <  2:1073871/91‑1 (MQ=255)
                                                            cacggctggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcgcg                                  <  1:2226811/93‑1 (MQ=255)
                                                              cggctggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCt                                <  2:3123550/95‑1 (MQ=255)
                                                               ggctggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg                               <  1:1242229/96‑1 (MQ=255)
                                                                  tggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc                            <  2:2593186/99‑1 (MQ=255)
                                                                  tggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc                            >  2:2214310/3‑101 (MQ=255)
                                                                  tggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc                            >  1:126989/3‑101 (MQ=255)
                                                                  tggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGACGCCGCGACGCCCGCGCTGATc                            <  2:1323500/99‑1 (MQ=255)
                                                                   ggCCGA.GTTGCTCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg                           >  1:2888696/2‑101 (MQ=255)
                                                                      ccgagttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCCAg                         >  2:1315414/10‑101 (MQ=255)
                                                                       cgagttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAgg                        <  1:3599867/93‑1 (MQ=255)
                                                                       cgagttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCCCGCTGATCGAgg                        >  1:4073944/9‑101 (MQ=255)
                                                                       cgagttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATACCGGGCGCCGCGACGCCCGCGCTGAACGAgg                        >  1:2108039/9‑101 (MQ=255)
                                                                        gagttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGa                       >  2:4371108/8‑101 (MQ=255)
                                                                        gagttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGa                       >  2:2208765/8‑101 (MQ=255)
                                                                         ggttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAt                      <  1:90616/95‑1 (MQ=255)
                                                                         agttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCGCGACGCCCCCGCTGATCGAGGAt                      >  2:4089098/7‑101 (MQ=255)
                                                                         agttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAt                      <  2:1120572/95‑1 (MQ=255)
                                                                         agttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAt                      >  1:810548/7‑101 (MQ=255)
                                                                          gttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtc                     <  2:3528520/96‑1 (MQ=255)
                                                                           ttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGGGCCGCGACGCCCGCGCTGATCGAGGAtct                    >  1:2794287/5‑101 (MQ=255)
                                                                           ttgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtct                    <  2:4242135/97‑1 (MQ=255)
                                                                            tgctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtctc                   <  1:4261277/98‑1 (MQ=255)
                                                                             gctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCg                  <  2:1404421/99‑1 (MQ=255)
                                                                              ctCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGa                 >  1:2588940/2‑101 (MQ=255)
                                                                              cccGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGa                 <  1:3026438/99‑1 (MQ=255)
                                                                               tCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAc                <  2:258957/101‑1 (MQ=255)
                                                                               tCGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAc                >  2:2930903/1‑101 (MQ=255)
                                                                                cGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGGTGATCGAGGAGCTCGAcc               >  2:2553826/1‑101 (MQ=255)
                                                                                cGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAcc               <  2:1676909/101‑1 (MQ=255)
                                                                                cGCCCGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAcc               <  1:1648406/101‑1 (MQ=255)
                                                                                   ccGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGTCGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcgcg            >  2:2993774/1‑101 (MQ=255)
                                                                                   ccGGCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcgcg            <  2:3185292/101‑1 (MQ=255)
                                                                                     ggCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGcc          >  2:3799793/1‑101 (MQ=255)
                                                                                     ggCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGcc          <  2:349554/101‑1 (MQ=255)
                                                                                      gCA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCg         >  2:2602528/1‑101 (MQ=255)
                                                                                       cA.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCgg        >  2:1092771/1‑101 (MQ=255)
                                                                                        a.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGc       >  2:4341316/1‑101 (MQ=255)
                                                                                        a.GGGCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGc       >  1:1135231/1‑101 (MQ=255)
                                                                                          gggCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGcc      >  1:2063906/1‑101 (MQ=255)
                                                                                           ggCGCGGGCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGccc     >  2:630188/1‑101 (MQ=255)
                                                                                              gcgggCA.CGGGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGGGCTGATCGAGGATCTCGACCGCGCCGGCCCCtc  >  1:3786013/4‑100 (MQ=255)
                                                                                                       |                                                                                             
CGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCA.GGGCGC.GGCA.C.GGCG.GCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCCCTA  >  NC_014034/3256135‑3256317

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG