BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,258,963	+C	100%	coding (778/1971 nt)	efeU →	ferrous iron permease EfeU

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,258,963	1	.→C	100.0%	61.2	234	coding (778/1971 nt)	efeU	ferrous iron permease EfeU
Reads supporting (aligned to +/- strand): new base (122/91): ref base (0/21): total (122/112)
Fisher's exact test strand distribution p-value = 6.50e-08
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.81e-02

Only 100 of 250 total aligned reads displayed.


CAATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCT  >  NC_014034/3258863‑3259057


cAATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.a                                                                                                 <  2:3642897/101‑1 (MQ=255)


cAATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGGCGCCCTCTGGGCCGCGTTCCTGGCCGTG.a                                                                                                 >  1:2677086/1‑101 (MQ=255)


  aTGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.cc                                                                                              <  2:1842500/101‑1 (MQ=255)


   tGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccc                                                                                             >  2:323809/1‑100 (MQ=255)


    gCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccct                                                                                            <  2:861382/101‑3 (MQ=255)


    gCCTCGGTCGCGGGCACCGTGCTTTCGACGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccct                                                                                            >  2:1283051/1‑99 (MQ=255)


      cTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGCCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccctgc                                                                                          >  1:190210/1‑97 (MQ=255)


       tCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccctgcc                                                                                         >  2:2929378/1‑96 (MQ=255)


         ggTCGCGGGCACGGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGc                                                                                        >  1:2422707/1‑101 (MQ=255)


         ggTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGc                                                                                        >  1:2710167/1‑101 (MQ=255)


         ggTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGc                                                                                        <  2:4191658/101‑1 (MQ=255)


           tCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGctc                                                                                      <  1:784423/101‑1 (MQ=255)


           tCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGctc                                                                                      <  2:3124471/101‑1 (MQ=255)


           tCGAGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGctc                                                                                      >  2:2223096/1‑101 (MQ=255)


            cgcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGctct                                                                                     >  2:78924/1‑101 (MQ=255)


             gcgGGCCCCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCtt                                                                                    <  1:2033758/101‑1 (MQ=255)


             gcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCtt                                                                                    >  2:1999363/1‑101 (MQ=255)


             gcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCtt                                                                                    >  2:4443950/1‑101 (MQ=255)


              cgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTc                                                                                   >  1:614198/1‑101 (MQ=255)


                ggCCCCGTGCTTTCGGCGCTCTTTGGCTATCCCGACAGCCCGACCCAGGGCGAGGTCCCCCTCTGGGCCCCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCt                                                                                 <  1:3969026/101‑1 (MQ=255)


                ggCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCt                                                                                 >  2:2074495/1‑101 (MQ=255)


                ggCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCt                                                                                 <  2:3904679/101‑1 (MQ=255)


                ggCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCt                                                                                 <  2:4135383/101‑1 (MQ=255)


                 gCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTg                                                                                <  2:2712526/101‑1 (MQ=255)


                  cACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTgc                                                                               >  2:4305451/1‑101 (MQ=255)


                    ccGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTgcgc                                                                             >  2:110630/1‑101 (MQ=255)


                       tGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcccc                                                                          <  2:3211038/101‑1 (MQ=255)


                        gCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCcg                                                                         >  2:1677993/1‑101 (MQ=255)


                        gCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCcg                                                                         <  1:4177242/101‑1 (MQ=255)


                           ttCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCgcgc                                                                      >  2:1591611/1‑101 (MQ=255)


                             cGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                    <  2:623584/101‑1 (MQ=255)


                             cGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                    >  1:4078229/1‑101 (MQ=255)


                             cGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                    <  1:2149591/101‑1 (MQ=255)


                             cGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                    <  1:4287757/101‑1 (MQ=255)


                              ggCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgcccg                                                                   <  2:2351934/101‑1 (MQ=255)


                               gcgcTCTCTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgcccgc                                                                  >  2:3415147/1‑101 (MQ=255)


                                 gcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCgcccgccc                                                                <  1:3368337/101‑1 (MQ=255)


                                 gcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCCCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCgcccgccc                                                                >  1:4402954/1‑101 (MQ=255)


                                  ctctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGcccc                                                               >  2:2978449/1‑101 (MQ=255)


                                  ctctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGcccc                                                               >  1:2162249/1‑101 (MQ=255)


                                   tctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGccccc                                                              <  1:2591091/101‑1 (MQ=255)


                                    ctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGcccccc                                                             >  2:368672/1‑101 (MQ=255)


                                    ctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGcccccc                                                             >  1:3861852/1‑101 (MQ=255)


                                     tttGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCt                                                            >  2:4346609/1‑101 (MQ=255)


                                        ggCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTcc                                                         >  1:4202572/1‑101 (MQ=255)


                                         gCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCg                                                        >  1:1198693/1‑101 (MQ=255)


                                          cTATCACGACAGCCCGACCCGGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGc                                                       <  2:2676914/101‑1 (MQ=255)


                                           tATCACGACAGCCCGACCCGGGGCGAGGTCGCCCTCTGGGCCTCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCa                                                      <  2:1154538/101‑1 (MQ=255)


                                           tATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCa                                                      >  1:2538749/1‑101 (MQ=255)


                                                 gACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGc                                                >  2:3595024/1‑101 (MQ=255)


                                                  aCAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGcc                                               <  2:929845/101‑1 (MQ=255)


                                                  aCAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGcc                                               >  1:1943732/1‑101 (MQ=255)


                                                   cAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCt                                              >  2:1012093/1‑101 (MQ=255)


                                                    aGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTg                                             >  1:3615212/1‑101 (MQ=255)


                                                    aGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTg                                             >  1:606039/1‑101 (MQ=255)


                                                    aGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTg                                             >  1:823199/1‑101 (MQ=255)


                                                     gCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGa                                            >  1:1703292/1‑101 (MQ=255)


                                                     gCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGa                                            >  2:2611354/1‑101 (MQ=255)


                                                      cccGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGAt                                           <  1:3885920/101‑1 (MQ=255)


                                                         gccccAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCg                                        <  2:3182132/99‑1 (MQ=255)


                                                         gACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCg                                        <  2:1298218/101‑1 (MQ=255)


                                                           cccAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGAt                                      <  1:4346880/101‑1 (MQ=255)


                                                            ccAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATg                                     <  2:816258/101‑1 (MQ=255)


                                                             cggggCGAGGTCGCCCTCGGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGa                                    <  1:937187/99‑1 (MQ=255)


                                                               gggCGAGGCCGCCCTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAcc                                  <  1:1656405/101‑1 (MQ=255)


                                                                ggCGAGGTCCCCCTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAccc                                 <  2:1959895/101‑1 (MQ=255)


                                                                  ggAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCt                               <  1:1020664/100‑1 (MQ=255)


                                                                  cggggTCGCCCTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCt                               <  1:3016385/98‑1 (MQ=255)


                                                                  cGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCt                               <  2:2426021/101‑1 (MQ=255)


                                                                  cGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCt                               <  2:2448083/101‑1 (MQ=255)


                                                                  cGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCt                               >  1:3130810/1‑101 (MQ=255)


                                                                   gAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                              >  1:1537463/1‑101 (MQ=255)


                                                                     ggccGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTcc                            <  1:3232663/98‑1 (MQ=255)


                                                                     ggTCCCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTcc                            <  1:4274018/101‑1 (MQ=255)


                                                                     ggTCCCCCTCGGGGCCGCGTCCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTcc                            <  2:3358978/101‑1 (MQ=255)


                                                                       tCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGc                          >  2:1555814/1‑101 (MQ=255)


                                                                           ccTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACaa                      <  2:4416083/101‑1 (MQ=255)


                                                                             tctGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgc                    <  1:2484342/101‑1 (MQ=255)


                                                                             tctGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgc                    >  1:2915491/1‑101 (MQ=255)


                                                                              ctGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgcg                   <  1:4233586/101‑1 (MQ=255)


                                                                               tGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgcgc                  >  2:2328908/1‑101 (MQ=255)


                                                                                  gCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGt               <  1:1443230/101‑1 (MQ=255)


                                                                                  gCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGt               >  1:278437/1‑101 (MQ=255)


                                                                                   ccGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTc              <  2:541523/101‑1 (MQ=255)


                                                                                    cgcgTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTcc             <  1:528322/101‑1 (MQ=255)


                                                                                     gcgTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCg            <  1:368711/101‑1 (MQ=255)


                                                                                       gTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCggg          >  2:2558729/1‑101 (MQ=255)


                                                                                       gTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCggg          >  2:3850458/1‑101 (MQ=255)


                                                                                        ttCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgc         <  2:3562127/101‑1 (MQ=255)


                                                                                        ttCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgc         <  2:251804/101‑1 (MQ=255)


                                                                                        ttCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgc         >  1:1076781/1‑101 (MQ=255)


                                                                                        ttCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgc         >  1:2247345/1‑101 (MQ=255)


                                                                                         tCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgcg        >  2:2149538/1‑101 (MQ=255)


                                                                                          ccTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgcgc       <  1:3487261/101‑1 (MQ=255)


                                                                                          ccTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgcgc       >  1:4426942/1‑101 (MQ=255)


                                                                                            tGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcct     >  1:3000882/1‑101 (MQ=255)


                                                                                            tGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcct     >  1:1309707/1‑101 (MQ=255)


                                                                                            tGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcct     <  2:2162110/101‑1 (MQ=255)


                                                                                             ggCCGTG.CCCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcctc    <  1:4116364/101‑1 (MQ=255)


                                                                                               ccGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcctcct  >  2:23960/1‑101 (MQ=255)


CAATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCT  >  NC_014034/3258863‑3259057

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG