Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,412,109 +G 2.3% intergenic (‑76/‑131) talC ← / → priA transaldolase/primosomal protein N`

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,412,1091.→G 2.3% 2.3 ‑3.9 ‑0.3129intergenic (‑76/‑131)talC/priAtransaldolase/primosomal protein N`
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (33/93):  total (36/93)
Fisher's exact test strand distribution p-value = 2.04e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.54e-01

Only 100 of 175 total aligned reads displayed.

TCGGCGGTATCGACGAAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGCAAATACGCCCG  >  NC_014034/3412008‑3412209
                                                                                                       |                                                                                                     
tCGGGGGTAT.GGCGAAAAATTTTCTTGAGGGGCCTCCTGCGTTCGGTCT.GCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCaa                                                                                                        <  1:2987010/101‑1 (MQ=255)
 cggcggTATCGACGAAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCaa                                                                                                        <  2:3864133/101‑1 (MQ=255)
 cggcggTATCGACGAAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCaa                                                                                                        >  1:2088104/1‑101 (MQ=255)
  ggcggTATCGACGAAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.g                                                                                                      >  1:792485/1‑101 (MQ=255)
     ggTATCGACGAAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.gggg                                                                                                   >  1:2478520/1‑101 (MQ=255)
      gTATCGACGAAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.ggggg                                                                                                  <  2:2226875/101‑1 (MQ=255)
       tATCGACGAAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.gggggg                                                                                                 >  2:3032701/1‑101 (MQ=255)
        aTCGACGAAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.ggggggg                                                                                                <  2:3742455/101‑1 (MQ=255)
         tCGACGAAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGc                                                                                               <  2:808067/101‑1 (MQ=255)
          cgacgaAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCt                                                                                              <  1:3340604/101‑1 (MQ=255)
            acgaAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.ggggggggctg                                                                                            >  1:2169793/1‑97 (MQ=255)
            acgaAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTg                                                                                            <  1:2741086/101‑1 (MQ=255)
             cgaAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.gggggagggggg                                                                                           >  1:722162/1‑94 (MQ=255)
             cgaAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTgc                                                                                           <  2:549351/101‑1 (MQ=255)
             cgaAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTgc                                                                                           <  2:1109714/101‑1 (MQ=255)
              gaagaa.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCNGAANCGGGCGCAA.GGGGGGGCTTgtg                                                                                          >  1:3975600/1‑99 (MQ=255)
              gaagaa.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.gggggggggtggc                                                                                          >  2:1214757/1‑95 (MQ=255)
               aagaa.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.ggggggggggggcg                                                                                         >  2:1699065/1‑94 (MQ=255)
                 gaa.TTTCATGAAGGGCGTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCGGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTgccgcc                                                                                       <  2:1898193/101‑1 (MQ=255)
                 gaa.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTgccgcc                                                                                       <  2:728844/101‑1 (MQ=255)
                  aa.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGccc                                                                                      <  1:635752/101‑1 (MQ=255)
                   a.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCGTgcgcgcgc                                                                                     >  2:289967/1‑95 (MQ=255)
                     tttCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGccccc                                                                                    <  1:1655945/101‑1 (MQ=255)
                      ttCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGcccccc                                                                                   >  1:1323192/1‑101 (MQ=255)
                      ttCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGcccccc                                                                                   <  1:3730329/101‑1 (MQ=255)
                       tCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCt                                                                                  <  2:276061/101‑1 (MQ=255)
                       tCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCt                                                                                  <  1:3168394/101‑1 (MQ=255)
                        cATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTc                                                                                 <  2:821709/101‑1 (MQ=255)
                         aTGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCg                                                                                <  1:1379547/101‑1 (MQ=255)
                            aaGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGGGTGGCGcccccccccccc                                                                             >  1:3668863/1‑95 (MQ=255)
                            aaGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGcc                                                                             >  2:487575/1‑101 (MQ=255)
                              gggCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTg                                                                           <  2:1068356/101‑1 (MQ=255)
                                gCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGAc                                                                         <  1:2662212/101‑1 (MQ=255)
                                 cctcctGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACg                                                                        <  1:3939276/101‑1 (MQ=255)
                                   tcctGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGc                                                                      <  2:2673203/101‑1 (MQ=255)
                                    cctGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTTGCCCCCCCCCCGGCCCGCCGGcc                                                                     >  1:2685935/1‑101 (MQ=255)
                                    cctGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACTGcc                                                                     <  2:2833472/101‑1 (MQ=255)
                                      tGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCctc                                                                   <  1:894475/101‑1 (MQ=255)
                                      tGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCctc                                                                   <  1:468831/101‑1 (MQ=255)
                                        cgcTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCtc.tc                                                                <  1:1509264/101‑1 (MQ=255)
                                          ctctGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.Tccc                                                              <  2:3839886/101‑1 (MQ=255)
                                           tctgtctgGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.Tcccc                                                             <  2:4070318/101‑1 (MQ=255)
                                            ctgtctgGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.Tccccc                                                            <  1:4210010/101‑1 (MQ=255)
                                                 tgGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCGTGACGGCCTC.TCCCCCcgcgc                                                       <  1:2539970/101‑1 (MQ=255)
                                                  ggCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAAGGGGGGGGTTTCCCCCCCCCCCCGCCCGGCCGGCCCC.Ccccccccccg                                                       >  1:1807066/1‑97 (MQ=255)
                                                  ggCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCCCGGCCTGACGGCCCC.Tccccccccccg                                                      >  1:1944687/1‑96 (MQ=255)
                                                  ggCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCCTTGCGCCCCCCCCCGCCCGACCGGCTC.Cccccccccccg                                                      >  2:3207670/1‑96 (MQ=255)
                                                   gCCGGGGTTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGt                                                     <  2:852575/101‑1 (MQ=255)
                                                    ccGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTa                                                    <  1:3479129/101‑1 (MQ=255)
                                                     cgcgATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTAt                                                   <  1:822449/101‑1 (MQ=255)
                                                     cgcgATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTAt                                                   <  1:840558/101‑1 (MQ=255)
                                                      gggATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTAtt                                                  <  2:1174480/99‑1 (MQ=255)
                                                      gcgATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTAtt                                                  <  1:1767039/101‑1 (MQ=255)
                                                      gcgATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCCGCCTGACGGGCCC.TCCCCCCCCCCGCAtt                                                  >  2:3514257/1‑101 (MQ=255)
                                                      gcgATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCCCGGCCTGACGGCCTC.TCCCCCCGCGCGGAtt                                                  >  1:4285099/1‑101 (MQ=255)
                                                        gATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTc                                                <  1:3908252/101‑1 (MQ=255)
                                                         aTTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGGTTGGCGCCCCCCCCCGCCCCGCCGCCCC.CCCCCCCCCGCGTAgtttt                                               >  2:3943563/1‑99 (MQ=255)
                                                         aTTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGTCCACCCCCTGGGCCTGCAGGGCTC.CCCCCCcgccccgattttg                                               >  2:2104596/1‑91 (MQ=255)
                                                          tttAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGGTTTCCCCCCCCCCCGCCCCGGCGGGCCC.CCCCCCCCCCCGCATTTccg                                              >  1:473511/1‑99 (MQ=255)
                                                          tttAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTCTTTCCCCcgcgcttccccgt                                               <  2:2467033/101‑9 (MQ=255)
                                                           ttAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGc                                             <  1:331075/101‑1 (MQ=255)
                                                            tAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGcc                                            <  2:311384/101‑1 (MQ=255)
                                                              gcgcAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCaa                                          <  1:1812651/101‑1 (MQ=255)
                                                              gcgcAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCaa                                          <  1:1462171/101‑1 (MQ=255)
                                                               cgcAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCaag                                         <  2:1548975/101‑1 (MQ=255)
                                                                gcAAACAGGCGGAAAAGGCGAGGGCGGAAACGGGCCGCA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCaaga                                        <  2:4024199/101‑1 (MQ=255)
                                                                gcAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCaaga                                        <  1:1035170/101‑1 (MQ=255)
                                                                 cAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGGCTGAGGGCCCC.CCCCCCCCCCCGGATTTCGGCCaaaaa                                       >  2:1825485/1‑101 (MQ=255)
                                                                 cAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCaagaa                                       <  1:1620812/101‑1 (MQ=255)
                                                                  aaaCAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCaagaag                                      <  1:126478/101‑1 (MQ=255)
                                                                  aaaCAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCaagaag                                      <  1:2356698/101‑1 (MQ=255)
                                                                    aCAGCGGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAgaagaa                                    <  1:4175446/101‑1 (MQ=255)
                                                                       gcgggAAATGGCGAGAGCGGAATCGGGCGCAG.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAAcc                                 <  2:2865805/98‑1 (MQ=255)
                                                                            aaaTGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGGCGCCCCCCCCGGGCTGACCGGCTC.TCCCCCCCGCCGCATTTCTGCCCGGAAGAAAACccccc                            >  1:2900417/1‑101 (MQ=255)
                                                                             aagggCGAGAGCGGAATCGGTCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAATAAACCccgccg                           <  2:1378724/98‑1 (MQ=255)
                                                                              agggCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGt                          <  2:3586879/99‑1 (MQ=255)
                                                                               tGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGGTTGGCGGCCCCCCCGGCCTCACGGCCCC.TCCCCCCGCGCGCATTTCGGCCCAGAAAAAACCccgcccct                         >  1:2759566/1‑98 (MQ=255)
                                                                                 gggagaGCGGAAGCGGGCGCAA.GGGGGGGGTTGCCCCCCCCCGCGGCCGGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTttc                       <  1:1214850/99‑1 (MQ=38)
                                                                                  cGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTttct                      <  1:2218250/101‑1 (MQ=255)
                                                                                  cGAGAGCGGAACCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTttct                      <  2:1660658/101‑1 (MQ=255)
                                                                                   gagaGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTttctt                     <  2:2635495/101‑1 (MQ=255)
                                                                                    agagCGGAATCGGGCGCAAGGGGGGGGTTTGCCCCCCCCCCCGGCCTGCGGGCCCT.CCCCCCCCCCCGATTTTTGCCCAAAAAAAAACCCCCCCCTttttt                     >  1:4388501/1‑101 (MQ=255)
                                                                                      ggCGGAATCGGGCGCAG.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAACCCCCGCCGTTTcttctt                  <  2:2083503/100‑1 (MQ=255)
                                                                                      agCGGAATCGGGCGCAG.GGGGGGGCTTCCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTcttctt                  <  2:3898825/101‑1 (MQ=255)
                                                                                      agCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTcttctt                  <  2:4318927/101‑1 (MQ=255)
                                                                                      agCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTcttctt                  <  2:456952/101‑1 (MQ=255)
                                                                                      agCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTcttctt                  <  2:581414/101‑1 (MQ=255)
                                                                                          gAATCGGGCCCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTg              <  2:592474/101‑1 (MQ=255)
                                                                                           aaTCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGCCGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGc             <  1:50138/101‑1 (MQ=255)
                                                                                            agcGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.CCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGCa            <  1:1721820/99‑1 (MQ=255)
                                                                                            aTCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCCGCCCGACGGCCCC.TCCCCCCCCGCGCATTTCGGCCAAGAAGAAAACCCGCCGCTTCTTCTTGGTGCa            >  2:1281353/1‑101 (MQ=255)
                                                                                             tCGGGCGCAA.GGGGGGGGTTTGCGCCCCCCCCCGCCCGACGGCCCC.TCCCCCCCCCCGGCTTTTCGCCAAAAAAAAACCCCCCCCGTTTTTCTTTGTGcca           >  2:2563478/1‑99 (MQ=255)
                                                                                              ggggCGCAG.GGGGGGGCTTGCCGCCCCCCTCGCCCTGACGGCCCC.CCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGCaaa          <  1:2549068/100‑1 (MQ=38)
                                                                                              cGGGCGCAG.GGGGGGCCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGCaaa          <  2:3447441/101‑1 (MQ=255)
                                                                                                ggCGCAG.GGGGGGGCTGCCCCCCCCCCTCGGCCTGACGGCCTC.CCCCCCCGCGCGTATTTCGGCCAAGAAGAACCCCCGCCGTTTCTTCTTGGTGCAAATa        <  1:3201721/101‑1 (MQ=38)
                                                                                                ggCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGCAAATa        <  1:4083861/101‑1 (MQ=255)
                                                                                                  cgcAA.GGGGGGGCTTCCCCCCCCCCTCGGCCTGACGGCCTC.CCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGCAAATAcg      <  2:3323401/101‑1 (MQ=255)
                                                                                                  cgcAA.GGGGGGCCTTCCCGCCCCCCTCGGCCTGACGGCCTC.CCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGCAAATAcg      <  2:911700/101‑1 (MQ=255)
                                                                                                   gcAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAACCCCCGCCGTTTCTTCTTGGTGCAAATAcgc     <  1:139277/101‑1 (MQ=255)
                                                                                                     aa.GGGGGGCCGTGCCCCCCCCCTCGGCCTGACGGCCTC.CCCCCCCGCGCGTATTTCGGCCAAGAAGAACCCCCGCCGTTTCTTCTTGGTGCAAATAcgccc   <  1:3424213/101‑1 (MQ=38)
                                                                                                       |                                                                                                     
TCGGCGGTATCGACGAAGAA.TTTCATGAAGGGCCTCCTGCGCTCTGTCTGGCCGCGATTTAGCGCAAACAGCCGGAAATGGCGAGAGCGGAATCGGGCGCAA.GGGGGGGCTTGCCGCCCCCCTCGGCCTGACGGCCTC.TCCCCCCGCGCGTATTTCGGCCAAGAAGAAACCCCGCCGTTTCTTCTTGGTGCAAATACGCCCG  >  NC_014034/3412008‑3412209

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG