BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,413,995	+G	2.0%	coding (1756/2184 nt)	priA →	primosomal protein N`

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,413,995	1	.→G	2.0%	2.1 ‑3.3 ‑0.1	149	coding (1756/2184 nt)	priA	primosomal protein N`
Reads supporting (aligned to +/- strand): new base (3/0): ref base (47/99): total (50/99)
Fisher's exact test strand distribution p-value = 3.63e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.67e-01

Only 100 of 194 total aligned reads displayed.


GGTCGGCGTGATCGACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACGAGGAGGCGTTCTGGAAGG  >  NC_014034/3413895‑3414094


ggTCGGCGTGATCGACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCgggcggggc                                                                                                         <  1:2937578/101‑1 (MQ=255)


 gTCGGCGTGATCGACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCgggcggggc.g                                                                                                       <  1:1431726/101‑1 (MQ=255)


   cggctggATCGACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCTGGCGGGGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGgcggggc.gg.g                                                                                                    <  2:3531532/95‑1 (MQ=255)


    ggCGTGATCGACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGGGGCGGGCCGGgcggggc.gg.gg                                                                                                   >  1:2829509/1‑100 (MQ=255)


    ggCGTGATCGACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGGGGCGGGCCGGgcggggc.gg.gg                                                                                                   >  1:2502936/1‑100 (MQ=255)


    ggCGTGATCGACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGANGCGGCAGGTGGGGGGGCGGgccgggc.gg.gg                                                                                                   >  1:486449/1‑100 (MQ=255)


       gTGATCGACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.a                                                                                               <  2:3267606/101‑1 (MQ=255)


        tGATCGACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGgg.gg.ggggga                                                                                               >  1:3586443/1‑99 (MQ=255)


         gATCGACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.aaa                                                                                             <  1:4114089/101‑1 (MQ=255)


          aTCGACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.aaaa                                                                                            <  1:660722/101‑1 (MQ=255)


          aTCGACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGCGCCGGGCGGGGC.GG.GGGG.aaaa                                                                                            >  2:1202273/1‑101 (MQ=255)


            cGACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGGGGCGGGCCGGGCGGGGC.GG.GGGG.gaaaaa                                                                                          >  1:2336031/1‑100 (MQ=255)


             gACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCCGGGG.GG.GCGG.GAAAAcc                                                                                         >  2:4238524/1‑101 (MQ=255)


                gCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GGAGGGG.AAAAAccgg                                                                                       >  1:2977435/1‑99 (MQ=255)


                   gATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCt                                                                                   <  1:1255951/101‑1 (MQ=255)


                   gATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCt                                                                                   <  2:3192063/101‑1 (MQ=255)


                   cgtCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCCCCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCt                                                                                   <  1:1630130/99‑1 (MQ=255)


                    aTCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGTC.GG.GCGG.AAAAACCCGGGCTg                                                                                  <  1:2539621/101‑1 (MQ=255)


                    aTCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTg                                                                                  <  2:3365848/101‑1 (MQ=255)


                      cTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGGGGCGGGCCGGGCGGGGCGGG.GGGG.AAAAGCCCggggggg                                                                                 >  1:3513605/1‑97 (MQ=255)


                       tGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGgcg                                                                               <  1:4047525/101‑1 (MQ=255)


                          gCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.tt                                                                           <  1:3324850/101‑1 (MQ=255)


                           ccTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.ttt                                                                          <  1:2199626/101‑1 (MQ=255)


                            cTGCAGNGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.tttn                                                                         <  1:301102/101‑2 (MQ=255)


                            cTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGGCGGGCGGGGC.GG.GGGG.GAAAAACCGGGGGGgcggcttt                                                                          >  1:739740/1‑96 (MQ=255)


                            cTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGGGGCGGGCCGGGCGGGGC.GG.GGGG.GAAAACCCGGGGTGGGGC.gttt                                                                         >  1:788832/1‑101 (MQ=255)


                             tGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTg                                                                        <  2:1684592/101‑1 (MQ=255)


                              gCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGGGGCGGGCCGGGCGGGGC.GG.GCGG.GAAAAACCGGGCGGGAGC.gttttc                                                                       >  2:486117/1‑99 (MQ=255)


                                 gggCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCTGGCGGGGCGGG.GGGG.AAAAACCCGGG.TGGCGC.CTTTTCAGa                                                                    >  1:1169146/1‑101 (MQ=255)


                                  ggCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGAc                                                                   <  1:3711164/101‑1 (MQ=255)


                                    cTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGCGGG.GGGG.AAAAACCCGGGTGGGGGC.tttttcgaccc                                                                  >  2:1023837/1‑94 (MQ=255)


                                     tCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTa                                                                <  1:3919392/101‑1 (MQ=255)


                                       cGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGGTGGAGC.TTTTGCCGACCTAta                                                              >  2:493245/1‑100 (MQ=255)


                                        gACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.GAAAAACCCGGCTGGCGG.TTTTGCCGGCCCATCa                                                             >  2:3551405/1‑101 (MQ=255)


                                        gACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCa                                                             <  1:245642/101‑1 (MQ=255)


                                         gccTGGGGGCGGCGGCGCGCCCTTTTCAACTGATGCGGCAGGTGGCGGGCCGGTCGGGCC.GG.GCGC.AAAACCCCGGGCTGGCGC.TTTTGCAGACCTATCAg                                                            <  2:3587782/100‑1 (MQ=255)


                                         aCCTGCGGGCGGCGGAGCGCGCCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAg                                                            <  1:1041195/101‑1 (MQ=255)


                                         aCCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.GAAAAACCGGGGGGGGGC.TTTTTCAGACCTATCAg                                                            >  1:3801697/1‑101 (MQ=255)


                                          ccTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GGGG.GAAAACCCCGGCTGGGGC.CTTTTCAGGCCCATCacc                                                           >  2:22470/1‑99 (MQ=255)


                                          ccTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGc                                                           <  2:2345129/101‑1 (MQ=255)


                                           cTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.GAAAACCCCGGCTGGCGC.TTTTGCAGACCTATAAGcc                                                          >  2:2298210/1‑101 (MQ=255)


                                           cTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGcc                                                          <  1:2310210/101‑1 (MQ=255)


                                            tGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGccc                                                         <  1:22483/101‑1 (MQ=255)


                                              cgggcggCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGa                                                       <  1:4238785/101‑1 (MQ=255)


                                              cgggcggCGGAGCGCACCTTTCAACTGATGCGGCAGGGGGCGGGCCGGGCGGGGC.GG.GCGGAAAAAACCCGGGC.GGGGC.TTTTTCAGACCTATccaaccca                                                       >  1:1541720/1‑94 (MQ=255)


                                                ggcggcGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GGGG.GAAAAACCGGGCTGGCGC.CTTTGCNGACCCATNNGCNCGCAc                                                     >  2:2120665/1‑101 (MQ=255)


                                                ggcggcGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAAc                                                     <  2:1413570/101‑1 (MQ=255)


                                                    gcggAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATcc                                                 <  1:2092669/101‑1 (MQ=255)


                                                     cggAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCg                                                <  1:1184137/101‑1 (MQ=255)


                                                     cggAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCg                                                <  2:4406656/101‑1 (MQ=255)


                                                     cggAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGACGGGCGGGGC.GG.GCGG.GAAAACCCCGGCTGGCGC.TTTTGCCGACCTATCAGCCCCAAAAACCg                                                >  2:1928040/1‑101 (MQ=255)


                                                      ggAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.GAAAACCCGGGGTGGCGC.TTTTGCAGACCTATCAGCCCGAAAATCCgg                                               >  1:3590387/1‑101 (MQ=255)


                                                        aGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.GAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTg                                             >  1:985605/1‑101 (MQ=255)


                                                          cgcACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GGGG.GAAAACCCGGGCTGGCGC.TTTTGCAGAACCATCAGCCCCAACACACCGTGAt                                           >  1:3054122/1‑101 (MQ=255)


                                                          cgcACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGAt                                           <  1:2702804/101‑1 (MQ=255)


                                                          cgcACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAAACCCGGCGGGCGC.GTTTGCAGACCCATCCGACCCAACCTCCGGGGAt                                           >  2:3514477/1‑101 (MQ=255)


                                                            cACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.GAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCCGGGATcc                                         >  1:554352/1‑101 (MQ=255)


                                                            cACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATcc                                         <  1:139084/101‑1 (MQ=255)


                                                             aCCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAAACCCGGCGGGGGC.TTTTGCAGACCTATTAGCCCGAACATTCAGGGATCcg                                        >  2:267429/1‑101 (MQ=255)


                                                               cTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCcgcg                                      <  1:442800/101‑1 (MQ=255)


                                                               cTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCcgcg                                      <  2:2829302/101‑1 (MQ=255)


                                                               cTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGAACcgcc                                      >  1:518530/1‑100 (MQ=255)


                                                                  tCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCa                                   <  2:1366912/101‑1 (MQ=255)


                                                                   cAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGGACATCCGGGGATTCCCGCCAt                                  >  2:946538/1‑101 (MQ=255)


                                                                   cAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCAt                                  <  1:4362746/101‑1 (MQ=255)


                                                                    aaCTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCACCAtt                                 <  2:3714754/101‑1 (MQ=255)


                                                                     aCTGATGCGGCTGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTGGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTc                                <  2:509494/101‑1 (MQ=255)


                                                                     aCTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTc                                <  2:1235038/101‑1 (MQ=255)


                                                                      cTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCt                               <  2:1255808/101‑1 (MQ=255)


                                                                      cTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCt                               <  1:3601356/101‑1 (MQ=255)


                                                                       tGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCtt                              <  1:486169/101‑1 (MQ=255)


                                                                        gATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.GAAAACCCGGGCTGGCGC.TTTTTCTGACCCATCAGCCCGAACATCCCGGGATCCGCGCCATTCTTg                             >  1:3409324/1‑101 (MQ=255)


                                                                        gATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTg                             <  1:1023922/101‑1 (MQ=255)


                                                                        gATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTg                             <  1:3278095/101‑1 (MQ=255)


                                                                         aTGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGGGATCCGCGCCCTTCTTgg                            >  2:3565906/1‑101 (MQ=255)


                                                                           gCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCg                          <  2:693686/101‑1 (MQ=255)


                                                                            cGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGCTTTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCg                          >  1:2711570/1‑101 (MQ=255)


                                                                             ggCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGGGATCCGCGCCATTCTTGGCggg                        >  2:1687448/1‑100 (MQ=255)


                                                                              gCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTg                       <  2:4201794/101‑1 (MQ=255)


                                                                                aGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGAc                     <  1:222719/101‑1 (MQ=255)


                                                                                aGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGAc                     <  1:2478262/101‑1 (MQ=255)


                                                                                aGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCCCGCCATTCTTGGCgggggc                     >  1:336723/1‑97 (MQ=255)


                                                                                   tGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACgag                  <  1:1129568/101‑1 (MQ=255)


                                                                                   tGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACgag                  <  1:2621216/101‑1 (MQ=255)


                                                                                    ggCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGGCgagg                 >  1:3385200/1‑101 (MQ=255)


                                                                                      cgggccgggcGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACgaggag               <  2:3467337/101‑1 (MQ=255)


                                                                                      cgggccgggcGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGGGATCCGCGCCATTCTTGGCGGTGACgaggag               <  1:4019077/101‑1 (MQ=255)


                                                                                       gggccgggcGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACGaggagg              >  1:3171746/1‑101 (MQ=255)


                                                                                        ggccgggcGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACGAGGAGGc             <  2:2502773/101‑1 (MQ=255)


                                                                                        ggccgggcGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACGAGGAGGc             <  1:946623/101‑1 (MQ=255)


                                                                                        ggccgggcGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGGGACGAGGAGGc             >  1:1265997/1‑101 (MQ=255)


                                                                                            gggcggggc.gG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACGAGGAGGCGTTc         <  2:3053901/101‑1 (MQ=255)


                                                                                            gggcggggc.gG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGGGACGAGGAGGCGGTc         >  2:159326/1‑101 (MQ=255)


                                                                                            gggcggggc.gG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCCATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACGAGGAGGCGTTc         <  2:3425400/101‑1 (MQ=255)


                                                                                               cggggc.gg.gCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACGAGGGGGCGGTCTgg      >  2:4024176/1‑101 (MQ=255)


                                                                                               cggggc.gg.gCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACGAGGAGGCGTTCTgg      >  1:4101709/1‑101 (MQ=255)


                                                                                               cggggc.gg.gCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACGAGGAGGCGTTCTgg      <  2:380268/101‑1 (MQ=255)


                                                                                                ggggc.gg.gCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACGAGGAGGCGTTCTGGa     >  1:1726068/1‑101 (MQ=255)


                                                                                                 gggc.gg.gcGG.AAAAACCCGGGCTGGCGC.TTTTGCAGATCTATCAGCCCCAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACGAGGAGGCGTTCTGGaa    <  2:2977214/101‑1 (MQ=255)


                                                                                                   gc.gg.gcgg.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACGAGGAGGCGTTCTGGAagg  <  2:911864/101‑1 (MQ=255)


GGTCGGCGTGATCGACGCCGATCTGGGCCTGCAGGGCTCCGACCTGCGGGCGGCGGAGCGCACCTTTCAACTGATGCGGCAGGTGGCGGGCCGGGCGGGGC.GG.GCGG.AAAAACCCGGGCTGGCGC.TTTTGCAGACCTATCAGCCCGAACATCCGGTGATCCGCGCCATTCTTGGCGGTGACGAGGAGGCGTTCTGGAAGG  >  NC_014034/3413895‑3414094

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG