Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,532,987 +G 90.3% intergenic (‑6/‑19) RCAP_rcc03340 ← / → RCAP_rcc03341 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,532,9871.→G90.3% 48.7 ‑3.3 ‑0.3175intergenic (‑6/‑19)RCAP_rcc03340/RCAP_rcc03341hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (83/75):  ref base (4/13):  total (87/88)
Fisher's exact test strand distribution p-value = 3.86e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.30e-01

Only 100 of 194 total aligned reads displayed.

AGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTC.G.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGC.G.GCCC..GCCCTTCGATCCAGTGCAGCCAACCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.CTGCGCGCCCGGCAGGGCCGCCAGATCGTGC  >  NC_014034/3532888‑3533080
                                                                                                       |                                                                                                 
aGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGc                                                                                                    >  2:2478782/1‑101 (MQ=255)
 gCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGC.g                                                                                                  <  1:991704/101‑1 (MQ=255)
  cGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGC.g.g                                                                                                >  1:4134548/1‑101 (MQ=255)
  cGGGGAAAGCGGCCCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGC.g.g                                                                                                <  2:1419701/101‑1 (MQ=255)
     ggAAAGCGGCCCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcc                                                                                             <  2:1086527/101‑1 (MQ=255)
      gAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggccc                                                                                            <  1:3781163/101‑2 (MQ=255)
       aaggcggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcccg                                                                                           <  2:2630036/98‑3 (MQ=255)
          gcggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcccggcc                                                                                        <  1:354704/101‑6 (MQ=255)
           cggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcccggccc                                                                                       <  2:1388874/101‑7 (MQ=255)
            ggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGC.g.ggcagatcgga                                                                                      >  1:3327681/1‑91 (MQ=255)
            ggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcccggccct                                                                                      <  1:740899/101‑8 (MQ=255)
            ggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcccggccct                                                                                      <  1:4125660/101‑8 (MQ=255)
            ggccCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcccggccct                                                                                      <  1:315555/101‑8 (MQ=255)
               gcCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCg                                                                                   <  2:206387/101‑1 (MQ=255)
                ccTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGa                                                                                  <  2:3306780/101‑1 (MQ=255)
                 cTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGAt                                                                                 <  2:3252759/101‑1 (MQ=255)
                  tGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATc                                                                                >  2:3915218/1‑101 (MQ=255)
                     cGGTCCAGCCGACCGAAAGCAGCCGCGCCTTGGCCAGCCGGGGGTC.GAGGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAg                                                                             >  2:2154891/1‑101 (MQ=255)
                      ggTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGt                                                                            >  1:1887363/1‑101 (MQ=255)
                       gcccAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTg                                                                           <  1:4035910/99‑1 (MQ=255)
                       gTCCAGCCGACCGAAAGCATCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTg                                                                           <  2:1249849/101‑1 (MQ=255)
                       gTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTg                                                                           <  1:1522652/101‑1 (MQ=255)
                         ccAGCCGCCCGAAAGCATCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCa                                                                         <  2:3739277/101‑1 (MQ=255)
                          cAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAg                                                                        >  1:3679337/1‑101 (MQ=255)
                           aGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGc                                                                       >  2:1020533/1‑101 (MQ=255)
                            gCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGcc                                                                      >  1:4017338/1‑101 (MQ=255)
                            gCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGcc                                                                      <  2:3488107/101‑1 (MQ=255)
                              cgaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCag                                                                    <  2:1725625/101‑2 (MQ=255)
                              cgaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCag                                                                    >  2:2142891/1‑100 (MQ=255)
                              cgaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCag                                                                    <  1:1471825/101‑2 (MQ=255)
                               gaccgaAACCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCagc                                                                   <  1:2094509/101‑3 (MQ=255)
                                  cgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGt                                                                <  2:190066/101‑1 (MQ=255)
                                    aaaGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTcc                                                              >  2:1706528/1‑101 (MQ=255)
                                    aaaGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTcc                                                              >  2:782572/1‑101 (MQ=255)
                                     aaGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCt                                                             >  1:3185268/1‑101 (MQ=255)
                                     aaGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCt                                                             <  1:1816196/101‑1 (MQ=255)
                                     aaGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGCCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCt                                                             >  2:2963688/1‑101 (MQ=255)
                                       gcagcCGCGCCTCGGCCAGCCGGGGGCCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCg                                                           >  2:2287977/1‑101 (MQ=255)
                                        cagcCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGa                                                          >  1:1783900/1‑101 (MQ=255)
                                          gcCGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGAcc                                                        >  1:4011198/1‑101 (MQ=255)
                                           ccGCGCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCg                                                       <  1:4213663/101‑1 (MQ=255)
                                            cgcgCCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGc                                                      >  1:641736/1‑101 (MQ=255)
                                             gcgcCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCa                                                     >  2:3542821/1‑101 (MQ=255)
                                              cgcCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCaa                                                    >  1:507847/1‑101 (MQ=255)
                                              cgcCTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCaa                                                    >  2:2007206/1‑101 (MQ=255)
                                                ccTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAcc                                                  <  2:3420400/101‑1 (MQ=255)
                                                 cTCGGCCGGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCg                                                 <  2:3109798/101‑1 (MQ=255)
                                                 cTCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCg                                                 >  2:3341353/1‑101 (MQ=255)
                                                  tCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGGCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGt                                                >  1:3983256/1‑101 (MQ=255)
                                                  tCGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCgc                                                >  1:3498676/1‑100 (MQ=255)
                                                   cGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTc                                               >  1:2443792/1‑101 (MQ=255)
                                                   cGGCCAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTc                                               <  1:2883477/101‑1 (MQ=255)
                                                      ccAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGcc                                            <  2:3004814/101‑1 (MQ=255)
                                                      ccAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGcc                                            >  1:794760/1‑101 (MQ=255)
                                                       cAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGccc                                           >  2:356578/1‑101 (MQ=255)
                                                       cAGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGccc                                           <  1:1549571/101‑1 (MQ=255)
                                                        aGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGcccc                                          >  2:2332376/1‑101 (MQ=255)
                                                        aGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGcccc                                          <  2:3461928/101‑1 (MQ=255)
                                                        aGCCGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGcccc                                          <  1:3364590/101‑1 (MQ=255)
                                                        aGCCGGGGGCCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCA.GCCCTTCGATCCAGTGCAGCCACCCGTCCTCGACCGCAACCCTCGcccc                                          >  1:3611169/1‑101 (MQ=255)
                                                          ccGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGc                                        <  2:4265304/101‑1 (MQ=255)
                                                           cGGGGGTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGca                                       >  1:123991/1‑101 (MQ=255)
                                                            gggggTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGccc                                      >  1:3429420/1‑99 (MQ=255)
                                                            gggggTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGcac                                      >  2:2734888/1‑101 (MQ=255)
                                                             ggggTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGcaca                                     <  2:4082680/101‑1 (MQ=255)
                                                             ggggTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGcaca                                     >  1:2322534/1‑101 (MQ=255)
                                                             ggggTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGcaca                                     <  2:2604558/101‑1 (MQ=255)
                                                              gggTCGG.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAg                                    <  1:216109/101‑1 (MQ=255)
                                                                gtcgg.ggg.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAATCGTCGCCCCGCACAGAt                                  >  2:4009380/5‑101 (MQ=255)
                                                                gTC.G.GGGTCGATCTGCGCCATCGCGGCGCAAAGCTGC.G.GCCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAt                                  <  2:4029126/101‑1 (MQ=255)
                                                                  cgg.ggg.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cc                               >  2:2189633/3‑100 (MQ=255)
                                                                  cgg.ggg.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cc                               >  2:257897/3‑100 (MQ=255)
                                                                   gg.ggg.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              <  2:2420709/100‑3 (MQ=255)
                                                                   gg.ggg.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              >  1:2486302/2‑99 (MQ=255)
                                                                   gg.ggg.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              >  1:1847914/2‑99 (MQ=255)
                                                                    g.ggg.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctg                             <  2:2536982/101‑4 (MQ=255)
                                                                    g.ggg.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctg                             <  2:3023538/101‑4 (MQ=255)
                                                                      ggg.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctgc                            <  1:3240427/101‑5 (MQ=255)
                                                                      ggg.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTAGACCGCAACCGTCGCCCCGCACAGAT.cctgc                            <  1:1998786/101‑5 (MQ=255)
                                                                       gg.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctgcg                           >  1:2967169/1‑96 (MQ=255)
                                                                       gg.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctgcg                           <  1:3005138/101‑6 (MQ=255)
                                                                       gg.CGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctgcg                           <  2:1847790/101‑6 (MQ=255)
                                                                           gATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGGGCAGCCACCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTgcgcgc                         >  1:3068633/1‑101 (MQ=255)
                                                                             tCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGccc                       <  2:2707151/101‑1 (MQ=255)
                                                                              cTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCg                      >  2:807207/1‑101 (MQ=255)
                                                                              cTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCg                      >  2:147896/1‑101 (MQ=255)
                                                                              cTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCg                      >  1:2786139/1‑101 (MQ=255)
                                                                               tGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCgg                     <  1:1397083/101‑1 (MQ=255)
                                                                               tGCGCCATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCgc                     >  1:1131258/1‑100 (MQ=255)
                                                                                   ccATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAgg                 <  2:3679084/101‑1 (MQ=255)
                                                                                   ccATCGCGGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAgg                 >  1:2270130/1‑101 (MQ=255)
                                                                                       cgcgGCGCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgccg             <  2:3614173/101‑1 (MQ=255)
                                                                                         cggcgCAAAGCTGC.GTGCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgccgcc           <  1:1047543/101‑1 (MQ=255)
                                                                                          ggggcAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCCCAGATCCTGCGCGCCCGGCAGGGCCGCCa          >  1:1688487/4‑101 (MQ=255)
                                                                                          ggcgCAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCa          <  2:2320253/101‑1 (MQ=255)
                                                                                           gcgcAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAg         <  2:1570954/101‑1 (MQ=255)
                                                                                            cgcAAAGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGa        <  1:2332526/101‑1 (MQ=255)
                                                                                              cagaGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATc      >  1:2558817/4‑101 (MQ=255)
                                                                                              cAAAGCTGC.G.GCCCCGGCCCTTCGATCCAGTGCAGACAGCCGTCCTGGACCGCAACCGTCGCCCCGCACAGCTCCTGCCCTCCCGGCACGGTCGTCacacc      >  2:456912/1‑97 (MQ=255)
                                                                                                aaGCTGCGG.GCCCG.GCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATCGt    >  2:564277/1‑101 (MQ=255)
                                                                                                       |                                                                                                 
AGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTC.G.GGG.CGATCTGCGCCATCGCGGCGCAAAGCTGC.G.GCCC..GCCCTTCGATCCAGTGCAGCCAACCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.CTGCGCGCCCGGCAGGGCCGCCAGATCGTGC  >  NC_014034/3532888‑3533080

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG