Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 586,917 +G 100% intergenic (‑145/+37) RCAP_rcc00548 ← / ← purL BolA family protein/phosphoribosylformylglycinamidine synthase II

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034586,9171.→G100.0% 28.1 155intergenic (‑145/+37)RCAP_rcc00548/purLBolA family protein/phosphoribosylformylglycinamidine synthase II
Reads supporting (aligned to +/- strand):  new base (74/70):  ref base (0/11):  total (74/81)
Fisher's exact test strand distribution p-value = 7.54e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.80e-02

Only 100 of 190 total aligned reads displayed.

GGCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.GGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAGCG  >  NC_014034/586817‑587012
                                                                                                       |                                                                                                
ggCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.c                                                                                                   <  2:774262/101‑1 (MQ=255)
 gCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.g                                                                                                 >  1:1859001/1‑101 (MQ=255)
  cGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gg                                                                                                <  1:1021562/101‑1 (MQ=255)
  cGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gg                                                                                                >  2:470785/1‑101 (MQ=255)
     cAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.ggggg                                                                                             >  1:1449963/1‑101 (MQ=255)
      aaGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggggg                                                                                            <  1:2486138/101‑1 (MQ=255)
      aaGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggggg                                                                                            >  2:1843104/1‑101 (MQ=255)
      aaGAGCCAAGCACCGGTTGCGTGAGCCAAGAGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggggg                                                                                            >  2:3585614/1‑101 (MQ=255)
       agagCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggggg.g                                                                                          >  2:3680588/1‑101 (MQ=255)
        gagCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggggg.gg                                                                                         <  1:760554/101‑2 (MQ=255)
        gagCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggggg.gg                                                                                         >  2:3882430/1‑100 (MQ=255)
             aaGCACCGTGTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGATGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCtt                                                                                     <  1:4388886/101‑1 (MQ=255)
             aaGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCtt                                                                                     >  1:475308/1‑101 (MQ=255)
             aaGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCtt                                                                                     >  1:3562515/1‑101 (MQ=255)
              aGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTc                                                                                    >  2:2154433/1‑101 (MQ=255)
               gccccGGTTGCGTGGGCCAAGTGACGCAGCGGGCGGGGGCCGCGGCGGGGGCGGGGGTGAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCt                                                                                   <  1:3664928/98‑1 (MQ=255)
                cACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTg                                                                                  <  1:4310158/101‑1 (MQ=255)
                cACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTg                                                                                  >  1:3936529/1‑101 (MQ=255)
                cACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCGTCTg                                                                                  >  2:3386440/1‑101 (MQ=255)
                 aCCGGTTGCGTGTGCCAAGTGACGCAGCGGGCGGGGGCCGCGGGGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTgc                                                                                 <  2:3632789/101‑1 (MQ=255)
                    ggTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTgcccg                                                                              >  1:3304165/1‑101 (MQ=255)
                     gTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTgcccgc                                                                             <  1:2782000/101‑1 (MQ=255)
                      ttGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTgcccgcc                                                                            >  2:1218599/1‑101 (MQ=255)
                       tGCGTGTGCCAAGGGACGCGGCGGGCGGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTgcccgccc                                                                           <  2:2290340/101‑1 (MQ=255)
                         ggtggGCCAAGGGGCGCAGGGGGCAGGGGCCGCGGCGAGGAGGGGGGGCGGGAGGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCg                                                                         <  2:2474131/100‑1 (MQ=255)
                          gtgtGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCgg                                                                        >  2:1685891/1‑101 (MQ=255)
                          gtgtGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCgg                                                                        <  2:2906738/101‑1 (MQ=255)
                           tgtgCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGa                                                                       >  1:829374/1‑101 (MQ=255)
                              gCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCt                                                                    >  1:2671885/1‑101 (MQ=255)
                                cAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTg                                                                  <  2:245353/101‑1 (MQ=255)
                                  aGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCg                                                                >  1:377894/1‑101 (MQ=255)
                                  aGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCg                                                                <  2:2431689/101‑1 (MQ=255)
                                   gTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGt                                                               >  1:3982088/1‑101 (MQ=255)
                                     ggcGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGGCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGttt                                                             <  1:4443134/99‑1 (MQ=255)
                                     gACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGttt                                                             >  1:1192827/1‑101 (MQ=255)
                                     gACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGttt                                                             >  1:2224599/1‑101 (MQ=255)
                                      gcGCAGCGGGCAGGGGCCGCGGGGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGtttt                                                            <  1:2209513/100‑1 (MQ=255)
                                      ccGCAGCGGGCGGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGtttt                                                            <  2:1373893/100‑1 (MQ=255)
                                       cGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTc                                                           <  2:380038/101‑1 (MQ=255)
                                          aGCGGGCGGGGGCCGCGGGGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGc                                                        <  1:2701423/101‑1 (MQ=255)
                                           gCGGGCAAGGGTCGCTGCTAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GTGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGcc                                                       >  2:1879858/1‑101 (MQ=255)
                                            ggggGAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAGGTGGCGCCGAGG.TGG.GGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCt                                                      <  2:645255/100‑1 (MQ=255)
                                             gggggggggCCGGGGGGGGGAGGGGGGTCGGGACGGGGGCAAGTGTCGCCGGGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCtt                                                     <  1:1178561/96‑1 (MQ=255)
                                              ggCGGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTc                                                    <  1:2970661/101‑1 (MQ=255)
                                               gCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                   >  2:1130577/1‑101 (MQ=255)
                                               gCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                   >  2:3133742/1‑101 (MQ=255)
                                               gCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                   >  2:4022597/1‑101 (MQ=255)
                                                gaGGGGCGGGGGCGGGGGCGGGGGGCAGGACGGGGGGAAGTGCCGCGGGGC.G.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGa                                                  <  2:2502270/100‑1 (MQ=255)
                                                cAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGGGCTTGCGTTTTTAGCCTTCGa                                                  >  2:2824365/1‑101 (MQ=255)
                                                 gggggCCGCGGCGAGGACGGGGGTCAGGAGGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAc                                                 <  2:530791/100‑1 (MQ=255)
                                                 gggcgCCGGGGCGAGGGGGGGGGTCGGGCCGGGGGAAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAc                                                 <  2:3635721/97‑1 (MQ=255)
                                                 aGGGGCCGCGGCGAGGGCGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAc                                                 <  1:3282734/101‑1 (MQ=255)
                                                 aGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAc                                                 >  2:4212140/1‑101 (MQ=255)
                                                 aGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAc                                                 >  1:3600507/1‑101 (MQ=255)
                                                      ccGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcggcgg                                            >  2:1895434/1‑101 (MQ=255)
                                                      ccGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcggcgg                                            >  1:2446120/1‑101 (MQ=255)
                                                          ggcgAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACggcggcggc                                        <  2:3981832/101‑1 (MQ=255)
                                                          ggcgAAGACGGGGGTCAGGACGGGGGCAAGTGTCGCCCAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACggcggcggc                                        <  1:3905038/101‑1 (MQ=255)
                                                           gcgAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGgcggcggcg                                       <  2:431860/101‑1 (MQ=255)
                                                           gcgAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGGTTTCAGCCTTCGACGgcggcggcg                                       >  1:2566742/1‑101 (MQ=255)
                                                            cgAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGa                                      >  1:4100612/1‑101 (MQ=255)
                                                              aGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAg                                    >  2:831615/1‑101 (MQ=255)
                                                              aGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAg                                    <  1:1400017/101‑1 (MQ=255)
                                                                gACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgc                                  <  2:1501069/101‑1 (MQ=255)
                                                                gACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgc                                  >  2:2184567/1‑101 (MQ=255)
                                                                  cGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcgg                                >  2:944234/1‑101 (MQ=255)
                                                                   gggggTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggt                               >  1:2052771/1‑101 (MQ=255)
                                                                    ggggTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtg                              <  2:3303933/101‑1 (MQ=255)
                                                                     ggggcgggACGGGGCCAAGCGCCCCCCACC.C.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgc                             <  1:1781561/95‑1 (MQ=255)
                                                                     gggACAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGCTTTCAGCCTTAGACGGCGGCGGCGAAGgcggtgc                             >  2:2792380/1‑101 (MQ=255)
                                                                      ggTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcg                            <  2:1413986/101‑1 (MQ=255)
                                                                       gTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcgg                           <  2:1449856/101‑1 (MQ=255)
                                                                        tCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcggt                          <  2:4089401/101‑1 (MQ=255)
                                                                        gcAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcggt                          <  2:1202829/100‑1 (MQ=255)
                                                                         cggggggggggGAAGTGGCGCCGAGC.TGG.GGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTa                         <  2:940138/95‑1 (MQ=255)
                                                                          aGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTaa                        >  2:1178953/1‑101 (MQ=255)
                                                                          aGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTaa                        >  2:4323437/1‑101 (MQ=255)
                                                                          aGGACGGGGCCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTaa                        <  2:2671713/101‑1 (MQ=255)
                                                                           ggAGGGGGGCAAGTGGCGCCGAGG.TGG.GGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTaaa                       <  2:3216569/101‑1 (MQ=255)
                                                                           ggACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTaaa                       <  2:394608/101‑1 (MQ=255)
                                                                             gggggggCAGGTGTCGCCGGGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGc                     <  1:617167/99‑1 (MQ=255)
                                                                             gggggggCAAGTGTCGCGGAGC.TGG.GGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGc                     <  1:544387/99‑1 (MQ=255)
                                                                              cGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGGGGGGCGGTAAAGCg                    >  2:1220724/1‑101 (MQ=255)
                                                                               gggggCAAGTGTCGCCGAGC.T.CGGGGGGG.GGGCTTCTGCCCGCCCCGGAGCTGGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGGGCGGTAAAGCgg                   >  2:3529030/1‑100 (MQ=255)
                                                                               gggggCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCgg                   >  2:1033739/1‑100 (MQ=255)
                                                                                ggggCAAGTGTCGCCGAGC.TGG.GGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTg                  <  1:3341107/101‑1 (MQ=255)
                                                                                ggggCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTg                  >  2:3136330/1‑101 (MQ=255)
                                                                                   gCAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTg               <  1:1239653/101‑1 (MQ=255)
                                                                                    caggtggCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGa              <  2:1561370/98‑1 (MQ=255)
                                                                                    cAAGTGTCGCCGAGCTT.G.GGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGa              <  1:572783/101‑1 (MQ=255)
                                                                                    cAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGa              >  2:163235/1‑101 (MQ=255)
                                                                                    cAAGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGGGCTgg              >  1:4114378/1‑100 (MQ=255)
                                                                                    cAAGTGGCGCCGGGC.G.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGa              <  1:2346052/101‑1 (MQ=255)
                                                                                     aaGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAg             >  1:2726473/1‑101 (MQ=255)
                                                                                     aaGTGTCGCCGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAg             >  1:1066607/1‑101 (MQ=255)
                                                                                         ggcGCCGAGC.TGG.GGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGctct         <  2:1066519/99‑1 (MQ=255)
                                                                                             ccGAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCa     <  1:4073020/101‑1 (MQ=255)
                                                                                              ggggC.T.C.GGGGGGCGCACTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAg    <  1:2962342/98‑1 (MQ=255)
                                                                                               gAGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAgc   >  1:3879468/1‑101 (MQ=255)
                                                                                                aGC.T.CGGGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAgcg  >  2:583757/1‑101 (MQ=255)
                                                                                                       |                                                                                                
GGCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.GGGGGG.GCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAGCG  >  NC_014034/586817‑587012

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG