Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	774,640	+C	100%	intergenic (‑94/+26)	oppB1 ← / ← oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	774,640	1	.→C	100.0%	81.3	21	intergenic (‑94/+26)	oppB1/oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (20/1):  ref base (0/0):  total (20/1)

CGGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCCGGT.CG.A.CG.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGC  >  NC_014034/774596‑774730                                              |                                                                                              gcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGt                                      >  2:420557/10‑101 (MQ=255)  caggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGtt                                     >  1:2718900/9‑101 (MQ=255)          gcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTAtg                             >  2:1383497/1‑100 (MQ=255)                      ccaggtcggaCCGGCCCTGCCGGTCCGTC.CC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCCGCCACGTCCCAGAATTGGCTGTCCCTgg                 >  2:2802417/10‑101 (MQ=255)                        gggtcggaCCGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCg               >  1:1567138/8‑101 (MQ=255)                        gggtcggaCCGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCg               >  1:2506449/8‑101 (MQ=255)                         ggtcggaCCGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGt              >  1:2647008/7‑101 (MQ=255)                          gtcggaCCGGCCCTGCCGGTCCG.ACCC.TACTGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGtt             >  1:3966371/6‑101 (MQ=255)                           tcggaCCGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTc            >  2:2107355/5‑101 (MQ=255)                            cggaCCGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa           >  1:1274061/4‑101 (MQ=255)                            cggaCCGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa           >  1:3308746/4‑101 (MQ=255)                             ggaCCGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt          >  1:1159198/3‑101 (MQ=255)                             ggaCCGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt          >  1:1334507/3‑101 (MQ=255)                             ggaCCGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt          >  1:3098029/3‑101 (MQ=255)                               cccGCCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtct        <  2:1211264/100‑1 (MQ=255)                               aCCGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGATCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtct        >  2:4046061/1‑101 (MQ=255)                                ccGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtctc       >  2:384458/1‑101 (MQ=255)                                ccGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtctc       >  1:2095916/1‑101 (MQ=255)                                 cGGCCCTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCg      >  2:4290952/1‑101 (MQ=255)                                    cccTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAcc   >  2:2078924/1‑100 (MQ=255)                                    cccTGCCGGTCCG.ACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACg   >  2:650032/1‑101 (MQ=255)                                     ccTGCCGGTCCG.A.CCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGc  >  2:675226/1‑101 (MQ=255)                                              |                                                                                              CGGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCCGGT.CG.A.CG.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGC  >  NC_014034/774596‑774730 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG