Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 905,035 +C 90.9% intergenic (+139/‑57) RCAP_rcc00821 → / → murB hypothetical protein/UDP‑N‑acetylmuramate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034905,0351.→C90.9% 37.5 ‑2.4 ‑1.2153intergenic (+139/‑57)RCAP_rcc00821/murBhypothetical protein/UDP‑N‑acetylmuramate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (78/61):  ref base (4/10):  total (82/71)
Fisher's exact test strand distribution p-value = 8.87e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.70e-02

Only 100 of 184 total aligned reads displayed.

GCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CG.CCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGCTACACCCCGCGCGGCGCGCTGAC  >  NC_014034/904935‑905129
                                                                                                          |                                                                                              
gCCAAAATTCACGCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAg.cg                                                                                                 >  1:2415032/1‑101 (MQ=255)
gCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAg.cg                                                                                                 >  1:1109769/1‑101 (MQ=255)
gCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAg.cg                                                                                                 >  2:1224017/1‑101 (MQ=255)
    aaaTTCACCCCCCGCCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CG.cccc                                                                                            <  2:168558/101‑1 (MQ=255)
     aaTTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGGAG.CG.ccccc                                                                                           >  2:3718714/1‑101 (MQ=255)
      aTTCCCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CG.cccccc                                                                                          <  1:2622039/101‑1 (MQ=255)
      aTTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGTAG.CG.cccccc                                                                                          >  2:170407/1‑101 (MQ=255)
      aTTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CG.cccccc                                                                                          >  1:4314246/1‑101 (MQ=255)
        tCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CG.cccccccc                                                                                        <  1:2213106/101‑1 (MQ=255)
          aCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGGCG.CG.cccccccccc                                                                                      >  2:884695/1‑99 (MQ=255)
          aCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CG.ccccccccct                                                                                      >  1:4216669/1‑99 (MQ=255)
           ccccccGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CG.CCCCCCCcctt                                                                                     >  1:4242531/1‑101 (MQ=255)
            cccccGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CG.CCCCCCCccttt                                                                                    >  2:1668733/1‑100 (MQ=255)
            cccccGGCGTATTGGCACCAGGAAAAAGCCCGGGGTTTTTCCTGGTC.GAAATCCG.CGGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CG.CCCCCCCccttt                                                                                    <  2:1003884/101‑2 (MQ=37)
               ccGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCctttctt                                                                                  <  2:2222329/101‑1 (MQ=255)
                cGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATCCG.CGGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCctttcttt                                                                                 <  2:3490689/101‑1 (MQ=37)
                cGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCctttcttt                                                                                 >  2:109118/1‑101 (MQ=255)
                  gtttATTGGCACCGAGAAAAAGCCCGGCGTTTTTCCTGGGC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCg                                                                               <  1:319119/98‑1 (MQ=25)
                  gCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCg                                                                               >  2:586241/1‑101 (MQ=255)
                   cGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGa                                                                              >  2:3599213/1‑101 (MQ=255)
                   cGTATTTCCACCAGGAAAAAGCCCGGCTTTGTTCCTGGTC.GAAATGCG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGa                                                                              <  1:303109/101‑1 (MQ=25)
                    gtttttGCCCCAGGAAAAAGGCCGGCCTTTTTCCCGGCC.CCAATACGCCCGGGCGGGACCCCGTCGTGC.GTGGGGGTCCCG.CC.CCCCCCCCTTTCTTTCGa                                                                              <  2:211508/98‑1 (MQ=255)
                    gTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGAc                                                                             >  1:3419095/1‑101 (MQ=255)
                    gTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGAc                                                                             >  1:1815174/1‑101 (MQ=255)
                      aTTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGAccc                                                                           >  1:78303/1‑101 (MQ=255)
                      aTTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGAC.GAAATACG.CTGTG.GGGAGTCCT.CTTGCGGACGGGGGGCAG.CGCCCCCCCCctttctttggcctc                                                                           >  2:1450790/1‑95 (MQ=25)
                       tttGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGAcccc                                                                          >  2:415669/1‑101 (MQ=255)
                        ttGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCt                                                                         >  2:2347218/1‑101 (MQ=255)
                        ttGCACCAGGAAAAAACCCGGCGTTTTTCCTGGTC.GAAATCCG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCt                                                                         <  2:1835019/101‑1 (MQ=37)
                         tGCGCCGGGGAAAGGCCGGGCGTTTGT.CTGGCCTGAAAAAGG.CAGGG.GGGGGCCCG.CTTGCGGGGGGGGGCCCG.CGCCCCCCCCCTTTCTTTCGACCCCtt                                                                        <  1:3631964/101‑1 (MQ=255)
                           cACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGc                                                                      >  1:3818167/1‑101 (MQ=255)
                           cACCA.GAAAAAACCCGGTTTTTTTCCGGGGCAAAAATGCG.CGGGG.GGGAGTCCG.TTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGc                                                                      <  1:884780/101‑1 (MQ=12)
                            cccAGGAAAAAGCCCTGTCTTTTTCCCTGTCAAAAATCCG.GGGGG.GGGAGTCCG.CTTGCGGAGGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGc                                                                      <  2:3732039/100‑1 (MQ=12)
                            aCCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGcc                                                                     >  1:906981/1‑101 (MQ=255)
                             ccAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGccc                                                                    >  2:726872/1‑101 (MQ=255)
                              cAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGcccc                                                                   >  2:3359047/1‑101 (MQ=255)
                               aGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGccccc                                                                  >  2:1922676/1‑100 (MQ=255)
                                ggAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCAt                                                                 <  2:4179214/101‑1 (MQ=255)
                                ggAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CG.CCCCCCCCTTTCTTTCGACCCCTTGCCCCAtt                                                                <  2:2935950/101‑1 (MQ=255)
                                ggAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGACCG.CGCCCCCCCCCTTTCTTTCGACCCCCTGCCCCAt                                                                 >  2:1789837/1‑101 (MQ=37)
                                    aaaGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTggg                                                             >  2:2736678/1‑101 (MQ=255)
                                    aaaGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAATCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGGCCCCTTGTCCCATTcgg                                                             >  1:2717358/1‑101 (MQ=25)
                                       gCCCGGCGTTTTTCCTGGTC.GAAAGACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTg                                                          <  2:3152485/101‑1 (MQ=255)
                                        cccGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGc                                                         <  1:3515428/101‑1 (MQ=255)
                                          cGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGctc                                                       <  2:3418247/101‑1 (MQ=255)
                                           ggCGTTTTTCCTGGTC.GAAATACG.CCTGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGGGctct                                                      >  1:1324264/1‑101 (MQ=37)
                                            gCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCtctc                                                     <  1:605094/101‑1 (MQ=255)
                                            gCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCtctc                                                     <  2:3639695/101‑1 (MQ=255)
                                             cGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCg                                                    >  1:2357289/1‑101 (MQ=255)
                                              gTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTCCGACCCCTTGCCCCATTGGGGTGCTCTCGt                                                   <  1:3974159/101‑1 (MQ=255)
                                               tttttCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGtt                                                  >  1:707404/1‑101 (MQ=255)
                                               tttttCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGtt                                                  >  1:1882792/1‑101 (MQ=255)
                                               tttttCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGGGCTCTCGtt                                                  >  2:3428275/1‑101 (MQ=255)
                                               ttttcCCTGGCC.AAAAGAGG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGtt                                                  <  2:47026/101‑1 (MQ=25)
                                                ttttCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTa                                                 <  2:2598252/101‑1 (MQ=255)
                                                ttttCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTa                                                 >  1:4139655/1‑101 (MQ=255)
                                                 tttCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTAt                                                >  1:473388/1‑101 (MQ=255)
                                                  ttCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATg                                               >  2:3374414/1‑101 (MQ=255)
                                                  ttCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGGGCTCTCGTTATg                                               >  1:4070886/1‑101 (MQ=255)
                                                   tCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATgg                                              >  1:2952247/1‑101 (MQ=255)
                                                       ggTC.GAAATCCG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAGCCC.CCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAgg                                          <  1:2208599/101‑1 (MQ=37)
                                                         tC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGc                                        >  2:854405/1‑101 (MQ=255)
                                                          c.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGGGCTCTCGTTATGGCAgggga                                       >  2:4319258/1‑99 (MQ=255)
                                                            gAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGGAt                                      >  2:3299445/1‑101 (MQ=255)
                                                            gAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCAt                                      >  1:1424121/1‑101 (MQ=255)
                                                            gAAATACG.CCGGG.GGGAGCCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCAt                                      <  2:3536473/101‑1 (MQ=255)
                                                              aaaacgcggggg.gggAGTCCG.CTGGCGGACGGGGGGCAGCCC.CCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATg                                     <  2:249919/92‑1 (MQ=255)
                                                                tACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGAc                                  <  1:1352453/101‑1 (MQ=255)
                                                                 aCG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGGATgggga                                 >  1:560761/1‑98 (MQ=37)
                                                                  cG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACaa                                <  2:588163/101‑1 (MQ=255)
                                                                   g.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAg                               <  2:3594849/101‑1 (MQ=255)
                                                                     ccGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGGTCTCGTTATGGCAGGGCATGGGCagac                              >  1:465677/1‑98 (MQ=25)
                                                                       ggg.gggGGTCCG.TTGCCGGCCGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTg                            <  2:1577959/101‑1 (MQ=255)
                                                                       ggg.gggAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGGATGGGCAAGCTg                            >  2:39918/1‑101 (MQ=37)
                                                                        gg.gggAGTCCG.CTTGGGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGa                           <  2:274504/101‑1 (MQ=255)
                                                                         g.gggAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGGGTGGGCAAGCTGAg                          >  1:2298146/1‑101 (MQ=37)
                                                                         g.gggAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGGATGGGCAAGCTggg                          >  2:1686164/1‑99 (MQ=37)
                                                                         g.gggAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACCAACTggg                          >  2:2844998/1‑99 (MQ=37)
                                                                         g.gggAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAg                          <  1:1224110/101‑1 (MQ=255)
                                                                           gggAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGc                         <  1:157246/101‑1 (MQ=255)
                                                                             gAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGGCAAGCTGAGCtc                       >  2:4288306/1‑100 (MQ=255)
                                                                             gAGTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGCTa                       <  1:1789956/101‑1 (MQ=255)
                                                                              ggTCCG.CTTGCGGACGGGGGGCAC.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGCTac                      <  1:2132956/100‑1 (MQ=255)
                                                                              aGTCCG.CTTGCGGACGGGGGGCAG.CG.CCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGGATGGGAAAGGTGAGCCaca                     >  1:1528749/1‑101 (MQ=37)
                                                                               gTCCG.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGGGTGGAACAGCTGGGGTacc                     >  2:655458/1‑100 (MQ=25)
                                                                                   g.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGCTACCcccc                 >  2:206100/1‑101 (MQ=255)
                                                                                   g.CTTGCGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGCTACAcccc                 <  1:2805550/101‑1 (MQ=255)
                                                                                         cGGACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGGTACAcccccccgg            >  2:3265332/1‑96 (MQ=255)
                                                                                          ggACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGCTACACCCCgcgcgg           >  1:1628903/1‑101 (MQ=255)
                                                                                          ggACGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCAGAGCTACACCCCccgcgg           >  1:1232831/1‑101 (MQ=255)
                                                                                            aCGGGGGGCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGGATGGACAAGCTGAGCTACACCCCccgcggcg         >  1:634746/1‑101 (MQ=255)
                                                                                            aCGGGGGGAAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGCTACACCCCgcgcggcg         <  1:2587110/101‑1 (MQ=255)
                                                                                              ggggggCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATCGGCAAGCTGAGCTACACCCCgcgcggggcc       >  2:1342146/1‑100 (MQ=255)
                                                                                               gggggCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATTGCACGGCATGGACAAGGTGAGCTACACCCCGCGCGgcgcgc      >  2:4422096/1‑101 (MQ=255)
                                                                                               gggggCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGCTACACCCCGCGCGgcgcgc      >  1:3767985/1‑101 (MQ=255)
                                                                                                ggggCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGCTACACCCCGCGCGGCGCGCt     <  1:3718967/101‑1 (MQ=255)
                                                                                                 gggCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGCTACACCCCGCGCGGCGCGCTg    <  1:3544194/101‑1 (MQ=255)
                                                                                                  ggCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGCTACACCCCGCGCGGCGCGCTGa   <  1:3359284/101‑1 (MQ=255)
                                                                                                  ggCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGCTACACCCCGCGCGGCGCGCTGa   <  2:796522/101‑1 (MQ=255)
                                                                                                   gCAG.CGCCCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGCTACACCCCGCGCGGCGCGCTGAc  <  2:1195220/101‑1 (MQ=255)
                                                                                                          |                                                                                              
GCCAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTC.GAAATACG.CCGGG.GGGAGTCCG.CTTGCGGACGGGGGGCAG.CG.CCCCCCCCTTTCTTTCGACCCCTTGCCCCATTGGGGTGCTCTCGTTATGGCAGGGCATGGACAAGCTGAGCTACACCCCGCGCGGCGCGCTGAC  >  NC_014034/904935‑905129

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG