Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,244,803 G→T 100% A294S (GCC→TCC)  RCAP_rcc01174 → D‑2‑hydroxyglutarate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,244,8030G→T100.0% 731.1 192A294S (GCC→TCC) RCAP_rcc01174D‑2‑hydroxyglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (107/85):  ref base (0/0):  total (107/85)

Only 100 of 199 total aligned reads displayed.

AACCGGCGCTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAAGCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATGCGCGAATC  >  NC_014034/1244705‑1244900
                                                                                                  |                                                                                                 
aaCCGGCGCTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATcc                                                                                                 >  2:2163001/1‑101 (MQ=255)
 gccGGCGCTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATccc                                                                                                >  2:143745/2‑101 (MQ=255)
  ccGGCGCTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCt                                                                                               >  2:785518/1‑101 (MQ=255)
   cGGCGCTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTg                                                                                              >  1:2518024/1‑101 (MQ=255)
    ggCGCTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGc                                                                                             <  2:1816507/101‑1 (MQ=255)
       gcTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTg                                                                                          <  2:4219733/101‑1 (MQ=255)
       gcTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTg                                                                                          <  2:4043561/101‑1 (MQ=255)
        cTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTgg                                                                                         >  1:722037/1‑101 (MQ=255)
        cTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTgg                                                                                         <  2:3646576/101‑1 (MQ=255)
         ttgCCTGTCCCGATGCGCCCCGCCCGTTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGgc                                                                                        <  1:1062746/99‑1 (MQ=255)
         tGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGgc                                                                                        <  1:3528688/101‑1 (MQ=255)
         tGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGgc                                                                                        <  2:627245/101‑1 (MQ=255)
          ggCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGgcg                                                                                       <  1:1988876/101‑1 (MQ=255)
          ggCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGgcg                                                                                       >  1:3690344/1‑101 (MQ=255)
             cTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGCGCCGCGCTGGAATCCCTGCTTGTCgcgg                                                                                    >  2:2812543/1‑101 (MQ=255)
              tGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACGACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCgcggc                                                                                   >  1:2168641/1‑101 (MQ=255)
              tGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCgcggc                                                                                   <  2:3997511/101‑1 (MQ=255)
              tGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCgcggc                                                                                   >  1:4437859/1‑101 (MQ=255)
               gTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCgcggcg                                                                                  >  1:746405/1‑101 (MQ=255)
               gTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCgcggcg                                                                                  <  1:3296829/101‑1 (MQ=255)
               gTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCgcggcg                                                                                  >  2:1729210/1‑101 (MQ=255)
                  ccTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATg                                                                               >  1:2642224/1‑101 (MQ=255)
                   cTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATgg                                                                              <  2:1684348/101‑1 (MQ=255)
                     ctGCGCCCCCCCCGGTCAATCTGCTGATCGAGAGCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAg                                                                            <  1:3863966/100‑1 (MQ=255)
                     aTGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAg                                                                            >  1:1596342/1‑101 (MQ=255)
                        cgccccgcccGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCg                                                                         <  1:2812749/101‑1 (MQ=255)
                              gcccgGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAg                                                                   >  1:127938/1‑101 (MQ=255)
                               cccgGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGa                                                                  <  2:2865168/101‑1 (MQ=255)
                               cccgGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGa                                                                  <  1:4249190/101‑1 (MQ=255)
                               cccgGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGa                                                                  <  1:1703548/101‑1 (MQ=255)
                                ccgGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGAAGAt                                                                 >  1:522405/1‑101 (MQ=255)
                                  ggTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCg                                                               >  1:439968/1‑101 (MQ=255)
                                    tCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGcc                                                             <  2:1596236/101‑1 (MQ=255)
                                    tCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGcc                                                             >  2:3710660/1‑101 (MQ=255)
                                     cAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGGAGATCTCCg                                                            >  1:2412411/1‑101 (MQ=255)
                                     cAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCg                                                            <  2:1302554/101‑1 (MQ=255)
                                     cAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCg                                                            >  2:2950590/1‑101 (MQ=255)
                                      aaTCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGGAGGTCGCCGa                                                           >  1:2537957/1‑101 (MQ=255)
                                      aaTCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCgg                                                           >  1:428071/1‑100 (MQ=255)
                                          tgctgATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCg                                                       >  2:3542478/1‑101 (MQ=255)
                                            ctgATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGt                                                     <  2:2440906/101‑1 (MQ=255)
                                              gATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCCAGGCGGTGa                                                   >  1:650796/1‑101 (MQ=255)
                                                tctagaCCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGAtt                                                 <  2:721957/98‑1 (MQ=255)
                                                tCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGAtt                                                 <  2:4064218/101‑1 (MQ=255)
                                                 cGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTg                                                <  2:2593319/101‑1 (MQ=255)
                                                 cGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTg                                                <  2:2910257/101‑1 (MQ=255)
                                                 cGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGATTg                                                >  2:513588/1‑101 (MQ=255)
                                                  gagaCCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGGAGATCGCCGAGGCGGTGGTTGc                                               >  2:3033379/1‑101 (MQ=255)
                                                  gagaCCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGATTGc                                               >  1:2888950/1‑101 (MQ=255)
                                                  gagaCCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATAGCCGAGGCGGGGGTTGc                                               >  2:3890514/1‑101 (MQ=255)
                                                  gagaCCGCGACCACCGTGCCCGGCGCCGATCCCGGGGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGGGGGGGAAATTTCCGAGGCggggcgtgc                                               >  1:18600/1‑94 (MQ=255)
                                                   agaCCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGATTGcc                                              >  1:4350110/1‑101 (MQ=255)
                                                     aCCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCa                                            <  1:1523863/101‑1 (MQ=255)
                                                     aCCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCa                                            <  1:2210486/101‑1 (MQ=255)
                                                     aCCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGATTGCCCa                                            >  2:1342210/1‑101 (MQ=255)
                                                      ccGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCaa                                           >  2:3794967/1‑101 (MQ=255)
                                                       cgcgACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCAGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAAt                                          >  2:188336/1‑101 (MQ=255)
                                                       cgcgACCACCGTGCCCGGCGCCCATCCCGGGGCCCCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAAt                                          <  1:1438544/101‑1 (MQ=255)
                                                        gcgACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATc                                         >  2:85729/1‑101 (MQ=255)
                                                         cgACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATcc                                        <  2:439928/101‑1 (MQ=255)
                                                          gACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGGGGTGATTGCCCAATCCg                                       >  2:3522633/1‑101 (MQ=255)
                                                            ccaccGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCgcc                                     <  2:4112471/101‑1 (MQ=255)
                                                              accGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCgccag                                   <  1:1819700/101‑1 (MQ=255)
                                                              accGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGATTGCCCAATCCgccag                                   >  1:227894/1‑101 (MQ=255)
                                                                cGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCgcccgcc                                 >  2:1214492/1‑101 (MQ=255)
                                                                cGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCgccagcc                                 <  2:3388247/101‑1 (MQ=255)
                                                                 gTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCgccagcca                                >  1:764192/1‑101 (MQ=255)
                                                                  tGCCCGGCGCCGATCCCGGTTCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGccagccag                               <  1:3033600/101‑1 (MQ=255)
                                                                  tGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGccagccag                               >  1:4368314/1‑101 (MQ=255)
                                                                   gCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCcagccagc                              >  1:2112856/1‑101 (MQ=255)
                                                                   gCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCcagccagc                              <  1:3537576/101‑1 (MQ=255)
                                                                   gCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCcagccagc                              <  1:1651529/101‑1 (MQ=255)
                                                                   gCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGATTGCCCAATCCGCcagccagc                              >  1:4076601/1‑101 (MQ=255)
                                                                    cccGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAgcg                             <  1:3078851/101‑1 (MQ=255)
                                                                    cccGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAgcg                             >  1:1379668/1‑101 (MQ=255)
                                                                     ccGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAgcgc                            >  2:2332049/1‑101 (MQ=255)
                                                                       ggCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGcgccg                          <  1:966888/101‑1 (MQ=255)
                                                                          gcCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCaa                       >  2:4159910/1‑101 (MQ=255)
                                                                            cGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGc                     >  1:4161822/1‑101 (MQ=255)
                                                                            cGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGc                     >  2:4277723/1‑101 (MQ=255)
                                                                                  tggTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTggg               >  1:2864178/2‑101 (MQ=255)
                                                                                  cGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTggg               <  1:1265613/101‑1 (MQ=255)
                                                                                   ggTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGga              >  2:3490570/1‑101 (MQ=255)
                                                                                   ggTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGga              >  1:984810/1‑101 (MQ=255)
                                                                                      gCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGAt           <  1:3085268/101‑1 (MQ=255)
                                                                                      gCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGAt           >  1:2921317/1‑101 (MQ=255)
                                                                                       ccGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATg          <  1:4056762/101‑1 (MQ=255)
                                                                                        cgcgCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATgc         <  1:2986941/101‑1 (MQ=255)
                                                                                        cgcgCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATgc         <  2:3102776/101‑1 (MQ=255)
                                                                                        cgcgCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATgc         >  1:3485804/1‑101 (MQ=255)
                                                                                         gcgcTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGGAGATCGCCCAGGCGGTGGTTGCCCAATCCGCCAGCCCGCGCCGCAAGCTGCGGGGGATgcg        >  1:627592/1‑101 (MQ=255)
                                                                                         gcgcTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCTCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCTCAAGCTGTGTGAGATgcg        <  2:3157779/101‑1 (MQ=255)
                                                                                          cgcTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATgcgc       >  2:3837336/1‑101 (MQ=255)
                                                                                           gcTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATGcgcg      >  1:4071384/1‑101 (MQ=255)
                                                                                           gcTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGATTGCCCAATCCGCCCGCCAGCGCCGCAAGCTGTGGGAGATGcgcg      >  1:802567/1‑101 (MQ=255)
                                                                                             tGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGGGATGCGCGaa    >  2:1661537/1‑101 (MQ=255)
                                                                                             tGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATGCGCGaa    >  2:1838797/1‑101 (MQ=255)
                                                                                             tGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGGTTGCCCAATCCGCCAGCCAGAGCCGCAAGCTGTGGGAGGTTGGCGaa    >  1:728135/1‑101 (MQ=255)
                                                                                              ggAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGGGGGAGATGCGCGAAt   >  1:1288828/1‑101 (MQ=255)
                                                                                               gAATCCCGGCTTGGCGCGGCGAGGGAGGCGGGGCAGATCGCCGAGGCGGTGATTCCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATGCGCGAATc  <  1:1009446/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AACCGGCGCTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAAGCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATGCGCGAATC  >  NC_014034/1244705‑1244900

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG