Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,247,167 G→C 9.1% A24A (GCC→GCG)  RCAP_rcc01176 ← GDSL‑like lipase/acylhydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,247,1670G→C 9.1% 5.7 ‑3.4 ‑0.1113A24A (GCC→GCG) RCAP_rcc01176GDSL‑like lipase/acylhydrolase
Reads supporting (aligned to +/- strand):  new base (12/0):  ref base (71/30):  total (83/30)
Fisher's exact test strand distribution p-value = 3.40e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.39e-01

Only 100 of 189 total aligned reads displayed.

TCCGCAACCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAGAC  >  NC_014034/1247067‑1247267
                                                                                                    |                                                                                                    
tCCGCAACCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCg                                                                                                      <  2:4140102/101‑1 (MQ=255)
   gCACCCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGcc                                                                                                   <  1:248428/101‑1 (MQ=255)
   gCAACCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGcc                                                                                                   <  1:3270699/101‑1 (MQ=255)
   gCAACCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGcc                                                                                                   <  1:4364601/101‑1 (MQ=255)
    cAACCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCcg                                                                                                  >  2:4355303/1‑101 (MQ=255)
     aaCCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGcccc                                                                                                 >  1:100477/1‑99 (MQ=255)
        cAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCGCACGGCCAGAAGCGTCACCGGCTCCGCCGCGGc                                                                                              >  1:3854755/1‑101 (MQ=255)
            ccTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGccccc                                                                                          <  2:3231105/101‑1 (MQ=255)
            ccTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCCCGGccccc                                                                                          >  2:3654891/1‑101 (MQ=255)
             cTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGcccccc                                                                                         <  1:1469205/101‑1 (MQ=255)
              tGCAGCTGCGGCACCAGCCCCTGATCCGGTTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGccccccc                                                                                        <  2:691976/101‑1 (MQ=255)
              tGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGACGCGGccccccc                                                                                        >  1:1441133/1‑101 (MQ=255)
              cgCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGccccccc                                                                                        <  2:2603758/100‑1 (MQ=255)
                 aGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGGCCCCccccc                                                                                     >  2:942287/1‑101 (MQ=255)
                  gCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCcccccg                                                                                    >  1:1375785/1‑101 (MQ=255)
                    tGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCccccccct                                                                                  >  1:548335/1‑98 (MQ=255)
                     gCGGCCCCAGCCCCTGATCCGGGTCCAGCCCGTCCCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATg                                                                                 <  1:554612/101‑1 (MQ=255)
                      cGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGc                                                                                >  2:3927056/1‑101 (MQ=255)
                        gCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGccc                                                                              <  2:3905371/101‑1 (MQ=255)
                        gCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCCATGccc                                                                              >  1:3397787/1‑101 (MQ=255)
                         ccccggCCCCTGATCCGGCTCCAGCCCGTGCCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCGCGGCCGCGGCCCCCCCGCCGATGCCCa                                                                             <  1:4381682/96‑1 (MQ=255)
                         ccccAGCCCCTGATCCGGGTCCAGCCCGTACCCCGGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCa                                                                             <  1:1167016/99‑1 (MQ=255)
                          cccAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAg                                                                            <  2:1755692/100‑1 (MQ=255)
                          aCCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCCGCCCCCCCGCCGATACCCAg                                                                            >  2:1791687/1‑101 (MQ=255)
                           ccAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCCGCCCCCCCCCCGATGCCCCgc                                                                           >  1:3625490/1‑101 (MQ=255)
                             aGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCGCGGGCCCCCCCCCCATGCCCAccgc                                                                         >  2:1198085/1‑101 (MQ=255)
                             aGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCCCCCACGGCCAGAAGCGTCACCGGCTCGGCCCCCGCCCCCCCCCCGACGCCCacccc                                                                         >  1:681614/1‑97 (MQ=255)
                              gCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCGCTGCCCACcgcg                                                                        >  2:1108091/1‑101 (MQ=255)
                              gCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCGCGGCCCCCCCCCCCCTGCCCCGcccg                                                                        >  1:1218298/1‑101 (MQ=255)
                               ccccTGACCCGGGTCCAGCCCGTCCCCCGGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGa                                                                       <  1:3194482/101‑1 (MQ=255)
                                 ccTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCCCGGCCCCCCCGCCGATGCCCCGCGCGaaa                                                                     >  1:450500/1‑101 (MQ=255)
                                 ccTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGCCGCCCACGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGaaa                                                                     <  2:4295000/101‑1 (MQ=255)
                                  cTGATCCGGGTCCAGCCCGTCCCCCGGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGaaaa                                                                    <  1:2239351/101‑1 (MQ=255)
                                  cTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCGCGGCCCCCCCGCCGCATCCCCGCGCGgaaa                                                                    >  2:161069/1‑101 (MQ=255)
                                   tGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGGCGCGGCCCCCCCGCCGATCCCCAgccggcacaa                                                                   >  1:3898428/1‑93 (MQ=255)
                                    gATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCCATGCCCAGCGCGAAAATc                                                                  >  2:3324795/1‑101 (MQ=255)
                                      cccGGGTCCAGCCCGTCCCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATccc                                                                <  2:3612389/100‑1 (MQ=255)
                                      cccGGGTCCAGCCCGTCCCCCTGGGTCAGGCGGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATccc                                                                <  2:2430621/100‑1 (MQ=255)
                                         gggTCCAGCCCGTCCCCCTGGGTCAGGCTGTCGCCCACGGCAAGAAGCTTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGc                                                             <  1:2690533/101‑1 (MQ=255)
                                         gggTCCAGCCCGTCCCCCTGGGTCAGGCTGCCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGc                                                             <  2:476166/101‑1 (MQ=255)
                                          ggTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCCAGGCCCCGCGCCAAAATACCCCCa                                                            >  2:1553561/1‑101 (MQ=255)
                                              cAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGGCCCCCCCCCGATGCCCCGCGCGAAAAACCCCCCAAgcc                                                        >  2:788432/1‑100 (MQ=255)
                                              cAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCGCCGCGGCCCCCCCCCCGACGCCCCGCGCGAAAAACCCCCCAAgcc                                                        >  1:3043487/1‑100 (MQ=255)
                                              cAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCCCCCCCGTCCCCCCCCCCGAAGCCCAGCGCGCGAACCCCCCCAAgac                                                        >  2:2862452/1‑99 (MQ=255)
                                               aGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGGCCCCCCGCCGATGCCCAGCGCGAAAAACCCCCCaaacac                                                       >  2:3360058/1‑97 (MQ=255)
                                               aGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGCTGCCCCGCGCGAAAAACCCCCCAAgcgc                                                       >  1:2471556/1‑101 (MQ=255)
                                               aGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAgcgc                                                       >  1:4165191/1‑101 (MQ=255)
                                                gCCCGTCCCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGcgcc                                                      <  1:3355015/101‑1 (MQ=255)
                                                 cccGTCCCCCTGGGTCAGGCTGCCGCCCAGGGCCAGAAGCGCCCCCGGCTCGGCCGCGCCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGcgccg                                                     <  2:4147284/101‑1 (MQ=255)
                                                   cGTCCCCCGGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCgccgcc                                                   <  1:1196994/101‑1 (MQ=255)
                                                    gtcccccTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCt                                                  <  2:2561900/98‑1 (MQ=255)
                                                    gtcccccGGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCt                                                  <  2:1725984/98‑1 (MQ=255)
                                                    gTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCCGCGCGAAAATCCCCGCAAGCGCCGCCt                                                  >  2:343200/1‑101 (MQ=255)
                                                      cccccTGGGTCAGGCTGTCGCCCGCGGCCAGAAGCGCCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTg                                                <  1:3094964/100‑1 (MQ=255)
                                                      cccccGGGGTCAGGCTGCCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTg                                                <  2:2351314/100‑1 (MQ=255)
                                                       ccccTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGc                                               >  1:2220149/1‑101 (MQ=255)
                                                        cccTGGGTCCGGCTGTCGCCCCCGGCCAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGcc                                              <  1:2215873/101‑1 (MQ=255)
                                                          cTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCCCAAGCGCCGCCCTGCCCa                                            >  1:726544/1‑101 (MQ=255)
                                                            gggTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCCGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCCgc                                          >  1:592718/1‑101 (MQ=255)
                                                             ggctgggCTGGCGCCCAGGGCCGGAAGCGTCCCCGGCCCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAgcc                                         <  1:3995696/96‑1 (MQ=255)
                                                             gaaaaGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATACCCAGCGCGAAAATCCCCGCAAGCGCCGGCTTGCCCAgcc                                         >  2:2992458/5‑101 (MQ=255)
                                                               tCAGGCTGTCGCCCACGGCCAGAAGCGCCACCGGCTCGGCCGCGGGCCCCCCCCCCAAGGCCAGCGGGGAAATCCCCCCAACGGCCCCCTTGCGCAgccgc                                       >  2:2932191/1‑101 (MQ=255)
                                                                cAGGCTGCCCCCCCCGGCAAGAAGCGTCCCCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAgccgcc                                      <  2:3854502/101‑1 (MQ=255)
                                                                 aGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCCCCGATGCCCAGCGCGAAAATCCCCCCAAGCGCCGCCTTGCCCAgccgccg                                     >  1:2767170/1‑101 (MQ=255)
                                                                     tGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCCCAAGCGCCGCCCTGCCCAGCcgccgccgc                                 >  2:1646984/1‑101 (MQ=255)
                                                                        cGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGGTGCCCAGCGCGAAAATCCCCGCAAGGGCCGCCTTCCCCAGCCGCGGCCGCAcc                              >  2:1158290/1‑101 (MQ=255)
                                                                        cGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCAcc                              >  2:2803635/1‑101 (MQ=255)
                                                                            cccGGCCAGACGCGCCACCGGCCCGGCCGGGCCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCat                          <  2:3129967/99‑1 (MQ=255)
                                                                            cagggCCGGAGGCGCCCCCGGCCCGGCCGCGCCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCat                          <  2:3441174/98‑1 (MQ=255)
                                                                            cACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCCGCGCGAAAATCCCCGCAAGCGCCGCCCTGCCCAGCCGCCGCCGCAccccct                          >  2:971386/1‑99 (MQ=255)
                                                                            cACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCat                          >  1:2135901/1‑101 (MQ=255)
                                                                             ccGGCCAGAAGCCTCCCCGGCGCGGCCGCGCCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCata                         <  1:2955830/100‑1 (MQ=255)
                                                                               ggCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATc                       >  1:2283515/1‑101 (MQ=255)
                                                                                gCCAGAAGCGTCCCCGGCCCGGCCCCGCCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATcc                      <  2:1462916/101‑1 (MQ=255)
                                                                                  cAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCCTATCCgg                    >  2:2233664/1‑101 (MQ=255)
                                                                                  cAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAACCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATcccg                    >  1:2182676/1‑99 (MQ=255)
                                                                                   aGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCACGCAAGCGCCGCCTTGCCCAGCCCCCGCCGCACCCCCTATCCGGt                   >  1:3730983/1‑101 (MQ=255)
                                                                                    gAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCCCAAGCGCCGCCTTGCCCAGACGCCGCCCCCCCCCCAATCCgggt                  >  1:4127685/1‑99 (MQ=255)
                                                                                      aGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGa                >  2:1120115/1‑101 (MQ=255)
                                                                                        cGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATc              >  1:3557319/1‑101 (MQ=255)
                                                                                         gcccccgccccGGCCGCGCCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATcc             <  1:788514/91‑1 (MQ=255)
                                                                                           cACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGGGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATcccc           >  2:2485027/1‑101 (MQ=255)
                                                                                           cACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATcccc           >  1:61700/1‑101 (MQ=255)
                                                                                              cGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCttt        >  1:3563023/1‑101 (MQ=255)
                                                                                               ggccgggCCGCGCCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTg       <  1:16434/96‑1 (MQ=255)
                                                                                               ggCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTg       >  2:606572/1‑101 (MQ=255)
                                                                                               ggCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTg       >  1:1320587/1‑101 (MQ=255)
                                                                                               ggCGCGGCCGCGCCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTg       <  2:1926399/101‑1 (MQ=255)
                                                                                                gCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGc      >  1:2378048/1‑101 (MQ=255)
                                                                                                 cTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCa     >  2:3535698/1‑101 (MQ=255)
                                                                                                 cTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTACa     >  2:3750450/1‑101 (MQ=255)
                                                                                                  tCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAg    >  1:1988330/1‑101 (MQ=255)
                                                                                                  ccGGCCGCGCCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAg    <  1:958712/100‑1 (MQ=255)
                                                                                                  ccGGCCGCGCCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAg    <  1:1016475/100‑1 (MQ=255)
                                                                                                   cGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAGa   >  1:1560275/1‑101 (MQ=255)
                                                                                                   cGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAGa   >  2:1288800/1‑101 (MQ=255)
                                                                                                   cGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAGa   >  2:3458741/1‑101 (MQ=255)
                                                                                                    ggCCGCGCCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAGAc  <  1:2052847/101‑1 (MQ=255)
                                                                                                    ggCCGCGCCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAGAc  <  1:2591288/101‑1 (MQ=255)
                                                                                                    ggCCGCGCCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAGAc  <  1:3211743/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
TCCGCAACCAGCCCTGCAGCTGCGGCACCAGCCCCTGATCCGGGTCCAGCCCGTACCCCTGGGTCAGGCTGTCGCCCACGGCCAGAAGCGTCACCGGCTCGGCCGCGGCCCCCCCGCCGATGCCCAGCGCGAAAATCCCCGCAAGCGCCGCCTTGCCCAGCCGCCGCCGCACCCCATATCCGGTTGATCCCCTTTGCAGAC  >  NC_014034/1247067‑1247267

Base quality scores:  ATCG  < 3 ≤  ATCG  < 8 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG