Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,283,564 A→G 100% T305A (ACG→GCG)  ppdK → pyruvate, phosphate dikinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,283,5640A→G100.0% 676.0 183T305A (ACG→GCG) ppdKpyruvate, phosphate dikinase
Reads supporting (aligned to +/- strand):  new base (94/89):  ref base (0/0):  total (94/89)

Only 100 of 192 total aligned reads displayed.

CGGTGCTCTGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACACGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGCTCTTGCGGGT  >  NC_014034/1283466‑1283661
                                                                                                  |                                                                                                 
 atctctctGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCgg                                                                                                <  2:3450021/97‑1 (MQ=255)
  gTGCTCTGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGt                                                                                               <  1:255723/101‑1 (MQ=255)
  gTGCTCTGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGt                                                                                               <  2:2311258/101‑1 (MQ=255)
   tGCTCTGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTg                                                                                              <  1:701822/101‑1 (MQ=255)
      tctGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCgg                                                                                           >  1:4219315/1‑101 (MQ=255)
      tctGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGGGCgg                                                                                           >  1:3986114/1‑101 (MQ=255)
       ctGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGACATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCggg                                                                                          <  2:292185/101‑1 (MQ=255)
        tGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGGGCgggg                                                                                         >  1:3820325/1‑100 (MQ=255)
          cGGGTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTgg                                                                                       <  1:343313/101‑1 (MQ=255)
           ggCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGgc                                                                                      >  1:2742175/1‑101 (MQ=255)
           ggCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGgc                                                                                      <  2:1213178/101‑1 (MQ=255)
           ggCCATGGCTCGGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGgc                                                                                      <  2:675024/101‑1 (MQ=255)
                tGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCt                                                                                 >  1:2411651/1‑101 (MQ=255)
                tGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCt                                                                                 >  1:284915/1‑101 (MQ=255)
                 ggCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGGTTTCGGTGATCGACGCGGGGCGGGGGgggcgccc                                                                                >  1:3032668/1‑99 (MQ=255)
                  gCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCg                                                                               >  1:2472475/1‑101 (MQ=255)
                   ctctGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCGCgg                                                                              >  2:2087550/1‑101 (MQ=255)
                    tctGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGc                                                                             >  1:1524046/1‑101 (MQ=255)
                    tctGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGc                                                                             <  2:3428923/101‑1 (MQ=255)
                    tctGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGGGCGGGGGGCGCGCTCGGc                                                                             >  1:3683277/1‑101 (MQ=255)
                     ctgcctgcCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGGGATCGACGCGGTGCGGGGGGCGCGCTCGGcc                                                                            >  2:2058794/1‑101 (MQ=255)
                       gcctgccGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCg                                                                          <  1:2326341/101‑1 (MQ=255)
                        cctgccGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCgg                                                                         <  2:2615703/101‑1 (MQ=255)
                        cctgccGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCgg                                                                         <  2:3283233/101‑1 (MQ=255)
                        cctgccGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCgg                                                                         <  2:785754/101‑1 (MQ=255)
                          tgccGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGGCCGGGc                                                                       >  2:417045/1‑101 (MQ=255)
                          tgccGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGc                                                                       <  1:2382607/101‑1 (MQ=255)
                             cGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGAc                                                                    <  1:476149/101‑1 (MQ=255)
                             cGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGAc                                                                    >  1:2199491/1‑101 (MQ=255)
                              gggCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCTACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACg                                                                   >  1:148617/1‑101 (MQ=255)
                              gggCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACg                                                                   >  2:2646639/1‑101 (MQ=255)
                              gggCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACg                                                                   >  2:4202147/1‑101 (MQ=255)
                               ggCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACgg                                                                  >  1:1629471/1‑101 (MQ=255)
                                gcgcGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGt                                                                 <  1:4437085/101‑1 (MQ=255)
                                 cgcgGCTGCGCGAGGAAATGCAGCTGGAATTGGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTg                                                                <  1:1457226/101‑1 (MQ=255)
                                   cgGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGaa                                                              <  2:455766/101‑1 (MQ=255)
                                   cgGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGaa                                                              <  1:3626370/101‑1 (MQ=255)
                                   cgGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGaa                                                              >  1:2047094/1‑101 (MQ=255)
                                   cgGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGaa                                                              <  1:188240/101‑1 (MQ=255)
                                    ggCTGGGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCCACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAg                                                             >  1:3274618/1‑101 (MQ=255)
                                    ggCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAg                                                             <  2:2805739/101‑1 (MQ=255)
                                    ggCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAg                                                             >  2:2404116/1‑101 (MQ=255)
                                    ggCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGGGAAg                                                             >  2:2187162/1‑101 (MQ=255)
                                     gCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGa                                                            >  1:1265760/1‑101 (MQ=255)
                                     gCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGa                                                            >  1:2498506/1‑101 (MQ=255)
                                      cTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGAt                                                           <  1:722214/101‑1 (MQ=255)
                                      cTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGAt                                                           <  2:1531601/101‑1 (MQ=255)
                                       tGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATc                                                          >  2:1362203/1‑101 (MQ=255)
                                         cgcgAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATcgc                                                        >  2:2717759/1‑101 (MQ=255)
                                            gAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGt                                                     >  1:2717623/1‑101 (MQ=255)
                                            gAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGGGAAGATCGCggg                                                     >  2:1609874/1‑100 (MQ=255)
                                             aGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCgggg                                                    >  2:4088570/1‑99 (MQ=255)
                                             aGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTg                                                    <  2:1012312/101‑1 (MQ=255)
                                             aGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTg                                                    <  1:2600513/101‑1 (MQ=255)
                                               gAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGc                                                  >  1:2991047/1‑101 (MQ=255)
                                                  aTGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCttt                                               >  1:2095000/1‑101 (MQ=255)
                                                   tGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTg                                              >  1:1008557/1‑101 (MQ=255)
                                                   tGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTg                                              >  1:494455/1‑101 (MQ=255)
                                                     cAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGc                                            <  1:1307411/101‑1 (MQ=255)
                                                     cAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGc                                            <  1:958422/101‑1 (MQ=255)
                                                     cAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGc                                            >  1:1925688/1‑101 (MQ=255)
                                                      aGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGcc                                           >  2:2247938/1‑101 (MQ=255)
                                                      aGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGcc                                           <  1:3749087/101‑1 (MQ=255)
                                                      aGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGcc                                           <  1:893237/101‑1 (MQ=255)
                                                       gCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCg                                          >  2:2727890/1‑101 (MQ=255)
                                                        cTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGa                                         <  1:3783545/101‑1 (MQ=255)
                                                         tGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAg                                        <  2:1258069/101‑1 (MQ=255)
                                                         tGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAg                                        >  1:432472/1‑101 (MQ=255)
                                                         tGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAg                                        <  1:2187301/101‑1 (MQ=255)
                                                         tGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCGTTGGCCGAg                                        >  2:1504744/1‑101 (MQ=255)
                                                          ggAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAgg                                       >  1:4174446/1‑101 (MQ=255)
                                                          ggAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAgg                                       <  1:112113/101‑1 (MQ=255)
                                                            aaTTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGGGGCTTTGGCCGAGGAc                                     >  2:3720495/1‑101 (MQ=255)
                                                              ttCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACgg                                   >  2:2149032/1‑101 (MQ=255)
                                                               tCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACggg                                  >  1:1168609/1‑101 (MQ=255)
                                                                cGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGa                                 <  1:663747/101‑1 (MQ=255)
                                                                 gcaaTTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGat                                <  2:3819446/98‑1 (MQ=255)
                                                                  ccATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGatc                               >  2:3382672/1‑101 (MQ=255)
                                                                   cATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGatca                              <  2:3582437/101‑1 (MQ=255)
                                                                    aTTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGatcat                             <  1:3050544/101‑1 (MQ=255)
                                                                      tGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGAtcatca                           >  1:4116644/1‑101 (MQ=255)
                                                                      tGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGAtcatca                           <  2:1913354/101‑1 (MQ=255)
                                                                        aaGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCAcc                         >  2:1869255/1‑101 (MQ=255)
                                                                        aaGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCAcc                         <  2:3826478/101‑1 (MQ=255)
                                                                         aGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCAccc                        <  1:1362300/101‑1 (MQ=255)
                                                                         aGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCAccc                        >  1:3931142/1‑101 (MQ=255)
                                                                               gcggATTTCGGTGATCGGCGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAg                  <  2:3342825/101‑1 (MQ=255)
                                                                                cggATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGa                 <  2:2488005/101‑1 (MQ=255)
                                                                                cggATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGa                 >  1:1857675/1‑101 (MQ=255)
                                                                                 ggATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGAt                >  2:4278448/1‑101 (MQ=255)
                                                                                 ggATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGAt                <  2:3683024/101‑1 (MQ=255)
                                                                                  gATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATg               <  1:3129624/101‑1 (MQ=255)
                                                                                   aTTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATgc              >  2:411916/1‑101 (MQ=255)
                                                                                     ttCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATgcgc            <  2:3982645/101‑1 (MQ=255)
                                                                                      tCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGct           <  2:3032447/101‑1 (MQ=255)
                                                                                       cGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGctc          <  1:3508720/101‑1 (MQ=255)
                                                                                           gATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGCTCTTGc      >  2:2690386/1‑101 (MQ=255)
                                                                                           gATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGCTCTTGc      >  1:2136774/1‑101 (MQ=255)
                                                                                            aTCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGCTCTTGCg     >  2:57970/1‑101 (MQ=255)
                                                                                               gACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGCTCTTGCGGGt  <  2:14802/101‑1 (MQ=255)
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CGGTGCTCTGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACACGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGCTCTTGCGGGT  >  NC_014034/1283466‑1283661

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG