Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,872 C→G 2.9% intergenic (+59/+98) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,8720C→G 2.9% 2.5 ‑2.1 ‑1.2138intergenic (+59/+98)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (61/72):  total (62/76)
Fisher's exact test strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.46e-02

Only 100 of 156 total aligned reads displayed.

CCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....GCCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAC  >  NC_014034/1303774‑1303965
                                                                                                       |                                                                                                    
ccTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAc                                                                                                      <  1:372764/101‑1 (MQ=37)
 cTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAcc                                                                                                     <  1:909789/101‑1 (MQ=37)
  tGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAccc                                                                                                    >  1:1415423/1‑101 (MQ=37)
   gTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAcccc                                                                                                   <  1:3090249/101‑1 (MQ=37)
    tCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCa                                                                                                  <  1:1356658/101‑1 (MQ=37)
    tCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCCGCCCCCC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAACCCa                                                                                                  >  1:3584021/1‑101 (MQ=37)
     cccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCTCCCCCGGGATATTTGGGGCAAGAT.AAACCCCAt                                                                                                 <  2:3617591/101‑1 (MQ=37)
     cccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCAt                                                                                                 <  1:981910/101‑1 (MQ=37)
      ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATc                                                                                                <  1:2094341/101‑1 (MQ=37)
      ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATc                                                                                                <  1:2504877/101‑1 (MQ=37)
        gcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCt                                                                                              <  1:1209386/101‑1 (MQ=37)
        gcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCt                                                                                              <  1:1717887/101‑1 (MQ=37)
         cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCtt                                                                                             <  2:3924512/101‑1 (MQ=37)
         cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCtt                                                                                             <  2:2294799/101‑1 (MQ=37)
         cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCGCACCCCCGGGATATTTGGGGCAAGAT.AAAACCCATCCtt                                                                                             >  2:2516216/1‑101 (MQ=25)
          gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCttt                                                                                            <  1:2266365/101‑1 (MQ=37)
          gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCttt                                                                                            >  2:549019/1‑101 (MQ=37)
          gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCttt                                                                                            >  2:954734/1‑101 (MQ=37)
           aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTc                                                                                           <  2:1655431/101‑1 (MQ=37)
            aaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCa                                                                                          <  1:2941568/101‑1 (MQ=37)
            aaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCa                                                                                          <  1:560586/101‑1 (MQ=37)
             aCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCAc                                                                                         <  2:1520131/101‑1 (MQ=37)
              cccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCAcc                                                                                        <  1:2909380/101‑1 (MQ=37)
               ccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCt                                                                                       <  2:1309633/101‑1 (MQ=37)
               ccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCt                                                                                       <  1:268955/101‑1 (MQ=37)
                cTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCtt                                                                                      <  2:1157380/101‑1 (MQ=37)
                 tGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTg                                                                                     <  1:1029661/101‑1 (MQ=37)
                 tGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTg                                                                                     <  1:2340026/101‑1 (MQ=37)
                  gcgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGc                                                                                    <  2:232175/101‑1 (MQ=37)
                  gcgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGc                                                                                    >  1:4069879/1‑101 (MQ=37)
                   cgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGcc                                                                                   <  2:709830/101‑1 (MQ=37)
                    gcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCg                                                                                  >  2:198702/1‑101 (MQ=37)
                    gcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCg                                                                                  >  1:381672/1‑101 (MQ=37)
                     cgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGc                                                                                 <  1:2484888/101‑1 (MQ=37)
                       ccGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAACCCATCCTTTCCCCTTGCCGCaa                                                                               >  1:2350921/1‑101 (MQ=25)
                         gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAat                                                                             >  1:325991/1‑101 (MQ=25)
                         gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAat                                                                             >  1:945980/1‑101 (MQ=25)
                         gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAat                                                                             <  1:548970/101‑1 (MQ=37)
                         gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGAAAat                                                                             <  2:603395/101‑1 (MQ=25)
                           ggTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCTCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAatat                                                                           <  1:4408920/101‑1 (MQ=37)
                           ggTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAatat                                                                           >  1:1970261/1‑101 (MQ=37)
                            gTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATc                                                                          <  1:4091011/101‑1 (MQ=37)
                            gTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATc                                                                          <  2:2894150/101‑1 (MQ=37)
                            gTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATc                                                                          <  2:4366074/101‑1 (MQ=37)
                              cAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCCCAAATATccc                                                                        >  1:4031346/1‑101 (MQ=25)
                                gCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCCCCCCCGGGGTATTTGGGGCAAGAT.AAACCCCCTCCTTTCACCTTGCCGCAAATATCCCgg                                                                      >  2:4200577/1‑101 (MQ=25)
                                 cGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                     >  2:3450970/1‑101 (MQ=25)
                                 cGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGAGATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                     >  1:3764368/1‑101 (MQ=37)
                                  gTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCgggg                                                                    <  1:2713752/101‑1 (MQ=37)
                                  gTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCgggg                                                                    >  2:1044292/1‑101 (MQ=37)
                                   tttAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCggggg                                                                   <  2:2141317/101‑1 (MQ=37)
                                    ttAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCgggggg                                                                  >  2:3877198/1‑101 (MQ=25)
                                      aGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCCCCTTGCCGCAAATATCCCGGGGGGTg                                                                <  2:2817443/101‑1 (MQ=25)
                                      aGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg                                                                >  1:2803911/1‑101 (MQ=37)
                                      aGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg                                                                >  2:3034117/1‑101 (MQ=25)
                                        gCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                              >  2:627859/1‑101 (MQ=25)
                                        gCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                              <  2:2298036/101‑1 (MQ=37)
                                        gCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGaa                                                              >  1:3093844/1‑101 (MQ=25)
                                         cAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGGCAGAT.AAACCCCATACTTTCACCTTGCCGCAAATATTCCGGGGGGGGact                                                             >  2:4417341/1‑99 (MQ=21)
                                         cAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt                                                             <  2:3835025/101‑1 (MQ=37)
                                         cAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt                                                             <  1:2281954/101‑1 (MQ=37)
                                          aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCGCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                            <  1:2705808/101‑1 (MQ=25)
                                          aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                            <  1:3714195/101‑1 (MQ=37)
                                          aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                            <  2:537103/101‑1 (MQ=37)
                                          aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAAtt                                                            >  1:953618/1‑101 (MQ=25)
                                          aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAACCCCATACCTGTTCCTTGCCACAAATATGCCgggggggcaact                                                            >  1:3837157/1‑95 (MQ=12)
                                          aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCACCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                            <  1:1772363/101‑1 (MQ=25)
                                           aGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                           <  2:2710675/101‑1 (MQ=37)
                                           aGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                           <  2:3421725/101‑1 (MQ=37)
                                           aGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAGTg                                                           >  2:3748250/1‑101 (MQ=25)
                                            gggggCTCTGC.CCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgcca                                                       <  2:4436892/101‑2 (MQ=2)
                                             ggggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgc                                                         >  2:2568582/1‑101 (MQ=37)
                                              gggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgcc                                                        >  1:423240/1‑101 (MQ=37)
                                              gggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgcc                                                        <  2:344965/101‑1 (MQ=37)
                                               ggCGCTG..CCCCCGCGGCTTCGCGACACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTGGCCGCAAATGTCCCGGGGGGTGAATTGgcc....g                                                   <  2:4177995/101‑1 (MQ=25)
                                                                                      tttGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcg                  >  1:3534568/1‑101 (MQ=14)
                                                                                       ttGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcgg                 <  2:4304026/101‑1 (MQ=14)
                                                                                        tGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGTGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                >  2:300071/1‑101 (MQ=9)
                                                                                        tGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                >  1:3138648/1‑101 (MQ=14)
                                                                                        tGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                >  2:3787186/1‑101 (MQ=14)
                                                                                        tGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                <  2:1970132/101‑1 (MQ=14)
                                                                                        tGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                <  2:3221761/101‑1 (MQ=14)
                                                                                        tGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                >  2:3016145/1‑101 (MQ=9)
                                                                                          gggCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc              >  2:806173/1‑101 (MQ=14)
                                                                                          gggCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc              <  2:4133103/101‑1 (MQ=14)
                                                                                          gggCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc              >  2:4154139/1‑101 (MQ=14)
                                                                                           ggCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccg             >  2:3622874/1‑101 (MQ=14)
                                                                                             cAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca           >  1:3356076/1‑101 (MQ=14)
                                                                                             cAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca           >  2:1556678/1‑101 (MQ=14)
                                                                                              aaGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcag          >  1:777618/1‑101 (MQ=14)
                                                                                               aGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg         >  2:1255868/1‑101 (MQ=14)
                                                                                               aGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg         >  1:2649734/1‑101 (MQ=14)
                                                                                               aGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg         >  2:3254270/1‑101 (MQ=14)
                                                                                               aGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg         >  2:2472281/1‑101 (MQ=14)
                                                                                               aGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg         <  1:203533/101‑1 (MQ=14)
                                                                                                 aT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggc       >  1:93677/1‑101 (MQ=14)
                                                                                                  t.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc      >  2:4247280/1‑101 (MQ=14)
                                                                                                    aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccggagggccc     >  2:2690958/1‑100 (MQ=9)
                                                                                                     aaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc    >  1:3966317/1‑101 (MQ=14)
                                                                                                     aaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc    >  2:1781408/1‑101 (MQ=14)
                                                                                                       |                                                                                                    
CCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....GCCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAC  >  NC_014034/1303774‑1303965

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG