Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,873 C→G 2.9% intergenic (+60/+97) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,8730C→G 2.9% 2.5 ‑2.1 ‑0.9138intergenic (+60/+97)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (62/72):  total (62/76)
Fisher's exact test strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.13e-01

Only 100 of 157 total aligned reads displayed.

CTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....GCCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACC  >  NC_014034/1303775‑1303966
                                                                                                       |                                                                                                    
cTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCCGGGTATTTGGGGCAAGAT.AAAc.c                                                                                                      >  2:3936609/1‑101 (MQ=37)
 tGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAc.cc                                                                                                     >  1:1415423/1‑101 (MQ=37)
 tGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAc.cc                                                                                                     >  1:2817650/1‑101 (MQ=37)
  gTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAc.ccc                                                                                                    >  1:675918/1‑101 (MQ=37)
   tCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCa                                                                                                   <  1:1356658/101‑1 (MQ=37)
     ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATc                                                                                                 <  1:2094341/101‑1 (MQ=37)
     ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATc                                                                                                 <  1:297474/101‑1 (MQ=37)
     ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATc                                                                                                 <  1:2504877/101‑1 (MQ=37)
       gcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCt                                                                                               <  1:1209386/101‑1 (MQ=37)
       gcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCt                                                                                               <  1:1717887/101‑1 (MQ=37)
        cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCtt                                                                                              <  2:3924512/101‑1 (MQ=37)
        cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCtt                                                                                              >  2:1236502/1‑101 (MQ=37)
         gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCttt                                                                                             >  2:954734/1‑101 (MQ=37)
         gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCttt                                                                                             >  2:549019/1‑101 (MQ=37)
         gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCttt                                                                                             <  1:2266365/101‑1 (MQ=37)
          aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                                                                            <  2:1153367/101‑1 (MQ=37)
          aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                                                                            <  1:898519/101‑1 (MQ=37)
          aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                                                                            <  1:4183610/101‑1 (MQ=37)
          aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAA.CCCATCATTTc                                                                                            >  2:1634156/1‑101 (MQ=25)
           aaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCa                                                                                           <  1:560586/101‑1 (MQ=37)
             cccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCAcc                                                                                         <  1:2909380/101‑1 (MQ=37)
              ccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCt                                                                                        <  2:1309633/101‑1 (MQ=37)
               cTGCGCGCCGTGGTCAGCGTTTAGGCGAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCtt                                                                                       <  1:2694111/101‑1 (MQ=25)
                tGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTg                                                                                      <  1:1029661/101‑1 (MQ=37)
                tGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTg                                                                                      <  1:2340026/101‑1 (MQ=37)
                 gcgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGc                                                                                     >  1:4069879/1‑101 (MQ=37)
                  cgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGcc                                                                                    <  2:709830/101‑1 (MQ=37)
                   gcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCg                                                                                   >  2:198702/1‑101 (MQ=37)
                    cgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGc                                                                                  <  1:2484888/101‑1 (MQ=37)
                      ccGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAA.CCCATCCTTTCCCCTTGCCGCaa                                                                                >  1:2350921/1‑101 (MQ=25)
                       cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCaaa                                                                               <  1:3252659/101‑1 (MQ=37)
                        gTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAat                                                                              >  1:325991/1‑101 (MQ=25)
                        gTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGAAAat                                                                              <  2:603395/101‑1 (MQ=25)
                          ggTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAatat                                                                            >  1:1970261/1‑101 (MQ=37)
                           gTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATc                                                                           <  2:4366074/101‑1 (MQ=37)
                             cAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATccc                                                                         <  2:1415316/101‑1 (MQ=37)
                             cAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCCCAAATATccc                                                                         >  1:4031346/1‑101 (MQ=25)
                              aGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCg                                                                        <  2:2251175/101‑1 (MQ=37)
                               gCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCCCCCCCGGGGTATTTGGGGCAAGAT.AAAC.CCCCTCCTTTCACCTTGCCGCAAATATCCCgg                                                                       >  2:4200577/1‑101 (MQ=25)
                                cGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                      >  2:3450970/1‑101 (MQ=25)
                                cGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                      <  2:1935375/101‑1 (MQ=37)
                                cGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGAGATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                      >  1:3764368/1‑101 (MQ=37)
                                 gTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggg                                                                     <  1:2713752/101‑1 (MQ=37)
                                 gTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggg                                                                     >  2:1044292/1‑101 (MQ=37)
                                  tttAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCTCCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggggg                                                                    <  1:3110089/101‑1 (MQ=37)
                                  tttAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggggg                                                                    <  2:2141317/101‑1 (MQ=37)
                                  tttAGGCAAGGGGGCGATG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggggg                                                                    >  1:4411521/1‑101 (MQ=25)
                                   ttAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggggg                                                                   >  2:3877198/1‑101 (MQ=25)
                                    tAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGt                                                                  <  1:3450426/101‑1 (MQ=37)
                                     cggacggggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAAt                                                              <  1:3652129/95‑1 (MQ=2)
                                     aGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCCCCTTGCCGCAAATATCCCGGGGGGTg                                                                 <  2:2817443/101‑1 (MQ=25)
                                     aGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg                                                                 >  2:3034117/1‑101 (MQ=25)
                                      ggCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGa                                                                <  1:94295/101‑1 (MQ=37)
                                       gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                               >  2:627859/1‑101 (MQ=25)
                                       gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                               <  2:2298036/101‑1 (MQ=37)
                                       gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                               <  1:2713358/101‑1 (MQ=37)
                                       gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGaa                                                               >  1:3093844/1‑101 (MQ=25)
                                        cAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGGCAGAT.AAAC.CCCATACTTTCACCTTGCCGCAAATATTCCGGGGGGGGact                                                              >  2:4417341/1‑99 (MQ=21)
                                        cAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt                                                              <  1:2281954/101‑1 (MQ=37)
                                        cAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt                                                              <  2:3835025/101‑1 (MQ=37)
                                        acggggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGG.GGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTgg                                                           <  2:3002218/98‑1 (MQ=2)
                                         aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                             <  2:537103/101‑1 (MQ=37)
                                         aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAAtt                                                             >  1:953618/1‑101 (MQ=25)
                                         aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAAC.CCCATACCTGTTCCTTGCCACAAATATGCCgggggggcaact                                                             >  1:3837157/1‑95 (MQ=12)
                                         aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCACCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                             <  1:1772363/101‑1 (MQ=25)
                                          aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                            <  2:2710675/101‑1 (MQ=37)
                                          aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                            <  2:3443337/101‑1 (MQ=37)
                                          aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                            <  2:3421725/101‑1 (MQ=37)
                                          aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAGTg                                                            >  2:3748250/1‑101 (MQ=25)
                                           gggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGG.GGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgcca                                                        <  2:4436892/101‑2 (MQ=2)
                                           gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                           >  2:3503051/1‑101 (MQ=37)
                                           gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                           <  1:3824735/101‑1 (MQ=25)
                                            ggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgc                                                          >  2:2568582/1‑101 (MQ=37)
                                             gggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgcc                                                         >  1:423240/1‑101 (MQ=37)
                                              ggCGCTG.CCCCCGCGGCTTCGCGACACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTGGCCGCAAATGTCCCGGGGGGTGAATTGgcc....g                                                    <  2:4177995/101‑1 (MQ=25)
                                                                                    tttGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcg                   >  1:3534568/1‑101 (MQ=14)
                                                                                    tttGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcg                   <  1:954819/101‑1 (MQ=14)
                                                                                     ttGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcgg                  <  2:4304026/101‑1 (MQ=14)
                                                                                      tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGTGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                 >  2:300071/1‑101 (MQ=9)
                                                                                      tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                 <  2:3221761/101‑1 (MQ=14)
                                                                                      tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                 >  2:3787186/1‑101 (MQ=14)
                                                                                      tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                 >  2:2885445/1‑101 (MQ=14)
                                                                                        gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc               >  2:806173/1‑101 (MQ=14)
                                                                                        gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc               >  1:3736375/1‑101 (MQ=14)
                                                                                        gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc               >  2:4154139/1‑101 (MQ=14)
                                                                                         ggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccg              >  2:3622874/1‑101 (MQ=14)
                                                                                           cAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca            >  2:1556678/1‑101 (MQ=14)
                                                                                             aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg          >  2:2472281/1‑101 (MQ=14)
                                                                                             aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg          >  1:2649734/1‑101 (MQ=14)
                                                                                             aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg          <  1:203533/101‑1 (MQ=14)
                                                                                             aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg          >  2:3254270/1‑101 (MQ=14)
                                                                                               aT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggc        >  1:93677/1‑101 (MQ=14)
                                                                                                t.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc       >  2:4247280/1‑101 (MQ=14)
                                                                                                t.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc       <  2:2480909/101‑1 (MQ=14)
                                                                                                  aaaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccggagggccc      >  2:2690958/1‑100 (MQ=9)
                                                                                                   aaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc     >  2:1781408/1‑101 (MQ=14)
                                                                                                   aaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc     >  1:3966317/1‑101 (MQ=14)
                                                                                                     c.cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc   >  2:2303029/1‑101 (MQ=14)
                                                                                                       cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAcc  >  1:486319/1‑101 (MQ=14)
                                                                                                       cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAcc  >  1:2702403/1‑101 (MQ=14)
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CTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....GCCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACC  >  NC_014034/1303775‑1303966

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG