Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,878 C→G 2.9% intergenic (+65/+92) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,8780C→G 2.9% 2.5 ‑2.1 ‑0.9138intergenic (+65/+92)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (65/69):  total (65/73)
Fisher's exact test strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.13e-01

Only 100 of 156 total aligned reads displayed.

CCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....G....CCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTC  >  NC_014034/1303780‑1303971
                                                                                                       |                                                                                                        
ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATc                                                                                                          <  1:2094341/101‑1 (MQ=37)
ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATc                                                                                                          >  1:2298181/1‑101 (MQ=37)
ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATc                                                                                                          <  1:2455505/101‑1 (MQ=37)
ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATc                                                                                                          <  1:297474/101‑1 (MQ=37)
  gcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCt                                                                                                        <  1:1209386/101‑1 (MQ=37)
  gcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCt                                                                                                        <  1:1717887/101‑1 (MQ=37)
   cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCtt                                                                                                       <  2:3924512/101‑1 (MQ=37)
   cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCtt                                                                                                       <  2:2294799/101‑1 (MQ=37)
   cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCGCACCCCCGGGATATTTGGGGCAAGAT.AAAA.CCCATCCtt                                                                                                       >  2:2516216/1‑101 (MQ=25)
    gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCttt                                                                                                      >  2:954734/1‑101 (MQ=37)
    gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCttt                                                                                                      <  1:2266365/101‑1 (MQ=37)
     aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                                                                                     >  1:1964968/1‑101 (MQ=37)
     aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                                                                                     <  2:1655431/101‑1 (MQ=37)
     aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                                                                                     <  1:4183610/101‑1 (MQ=37)
     aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                                                                                     <  1:898519/101‑1 (MQ=37)
     aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                                                                                     <  2:1153367/101‑1 (MQ=37)
     aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAA.CCCATCATTTc                                                                                                     >  2:1634156/1‑101 (MQ=25)
      aaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCa                                                                                                    <  1:560586/101‑1 (MQ=37)
      aaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCa                                                                                                    <  1:2941568/101‑1 (MQ=37)
       aCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCAc                                                                                                   <  2:1520131/101‑1 (MQ=37)
       aCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCAc                                                                                                   <  1:4067251/101‑1 (MQ=37)
        cccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCAcc                                                                                                  <  1:2909380/101‑1 (MQ=37)
          cTGCGCGCCGTGGTCAGCGTTTAGGCGAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCtt                                                                                                <  1:2694111/101‑1 (MQ=25)
          cTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCtt                                                                                                <  2:1157380/101‑1 (MQ=37)
           tGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTg                                                                                               <  1:1029661/101‑1 (MQ=37)
           tGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTg                                                                                               <  1:2340026/101‑1 (MQ=37)
            gcgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGc                                                                                              <  2:232175/101‑1 (MQ=37)
             cgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGcc                                                                                             <  2:709830/101‑1 (MQ=37)
              gcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCg                                                                                            >  1:381672/1‑101 (MQ=37)
               cgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGc                                                                                           <  1:2484888/101‑1 (MQ=37)
                  cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCaaa                                                                                        >  2:676889/1‑101 (MQ=37)
                  cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCaaa                                                                                        <  1:2567733/101‑1 (MQ=37)
                   gTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAat                                                                                       >  1:945980/1‑101 (MQ=25)
                   gTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAat                                                                                       >  1:325991/1‑101 (MQ=25)
                   gTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGAAAat                                                                                       <  2:603395/101‑1 (MQ=25)
                     ggTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCTCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAatat                                                                                     <  1:4408920/101‑1 (MQ=37)
                     ggTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAatat                                                                                     >  1:1970261/1‑101 (MQ=37)
                      gTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATc                                                                                    <  1:4091011/101‑1 (MQ=37)
                      gTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATc                                                                                    <  2:2894150/101‑1 (MQ=37)
                      gTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATc                                                                                    <  2:4366074/101‑1 (MQ=37)
                       tCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATcc                                                                                   <  2:3109187/101‑1 (MQ=37)
                        cAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATccc                                                                                  <  2:1415316/101‑1 (MQ=37)
                           cGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                               <  2:1935375/101‑1 (MQ=37)
                             tttAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCTCCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggggg                                                                             <  1:3110089/101‑1 (MQ=37)
                             tttAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggggg                                                                             <  2:2141317/101‑1 (MQ=37)
                             tttAGGCAAGGGGGCGATG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggggg                                                                             >  1:4411521/1‑101 (MQ=25)
                              ttAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggggg                                                                            >  2:3877198/1‑101 (MQ=25)
                               tAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGt                                                                           <  1:3450426/101‑1 (MQ=37)
                                aGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCCCCTTGCCGCAAATATCCCGGGGGGTg                                                                          <  2:2817443/101‑1 (MQ=25)
                                aGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg                                                                          >  1:2803911/1‑101 (MQ=37)
                                 ggCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGa                                                                         <  2:433091/101‑1 (MQ=37)
                                 ggCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGa                                                                         <  1:94295/101‑1 (MQ=37)
                                  gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                                        <  1:2713358/101‑1 (MQ=37)
                                   cAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGGCAGAT.AAAC.CCCATACTTTCACCTTGCCGCAAATATTCCGGGGGGGGact                                                                       >  2:4417341/1‑99 (MQ=21)
                                   cAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt                                                                       <  2:3835025/101‑1 (MQ=37)
                                   acggggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGG.GGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTgg                                                                    <  2:3002218/98‑1 (MQ=2)
                                    ctgggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                      <  1:2093029/99‑1 (MQ=25)
                                    aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                      <  2:537103/101‑1 (MQ=37)
                                    aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                      <  1:3714195/101‑1 (MQ=37)
                                     aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                                     <  2:3443337/101‑1 (MQ=37)
                                     aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                                     <  2:2710675/101‑1 (MQ=37)
                                     aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAGTg                                                                     >  2:3748250/1‑101 (MQ=25)
                                      gggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGG.GGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgcca                                                                 <  2:4436892/101‑2 (MQ=2)
                                      gggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgcca                                                                 <  2:4155742/101‑2 (MQ=2)
                                      gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                                    >  2:3503051/1‑101 (MQ=37)
                                      gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                                    <  2:337839/101‑1 (MQ=37)
                                      gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAAGATCCCGGGGGGTGAAtgag                                                                    >  2:2092892/1‑98 (MQ=25)
                                      gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                                    <  1:3824735/101‑1 (MQ=25)
                                        gggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgcc                                                                  <  2:344965/101‑1 (MQ=37)
                                         ggCGCTG.CCCCCGCGGCTTCGCGACACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTGGCCGCAAATGTCCCGGGGGGTGAATTGgcc....g                                                             <  2:4177995/101‑1 (MQ=25)
                                                                               tttGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcg                        >  1:3534568/1‑101 (MQ=14)
                                                                               tttGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcg                        <  1:954819/101‑1 (MQ=14)
                                                                                 tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                      >  1:3138648/1‑101 (MQ=14)
                                                                                 tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                      >  2:3787186/1‑101 (MQ=14)
                                                                                 tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                      <  2:3221761/101‑1 (MQ=14)
                                                                                 tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                      >  2:2885445/1‑101 (MQ=14)
                                                                                 tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                      <  2:1970132/101‑1 (MQ=14)
                                                                                 tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                      >  2:3016145/1‑101 (MQ=9)
                                                                                   gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                    >  1:3736375/1‑101 (MQ=14)
                                                                                   gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                    <  2:4133103/101‑1 (MQ=14)
                                                                                    ggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccg                   >  2:3622874/1‑101 (MQ=14)
                                                                                        aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg               >  2:1255868/1‑101 (MQ=14)
                                                                                        aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg               >  1:2649734/1‑101 (MQ=14)
                                                                                           t.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc            <  2:2480909/101‑1 (MQ=14)
                                                                                           t.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc            >  2:4247280/1‑101 (MQ=14)
                                                                                             aaaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccggagggccc           >  2:2690958/1‑100 (MQ=9)
                                                                                             aaaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg           >  1:1427834/1‑101 (MQ=14)
                                                                                             aaaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg           >  2:423736/1‑101 (MQ=14)
                                                                                             aaaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg           >  2:560068/1‑101 (MQ=14)
                                                                                              aaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc          >  1:3966317/1‑101 (MQ=14)
                                                                                               aC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgca         >  1:3863071/1‑101 (MQ=14)
                                                                                                c.cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc        >  2:2303029/1‑101 (MQ=14)
                                                                                                  cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAcc       >  1:2702403/1‑101 (MQ=14)
                                                                                                  cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAcc       >  1:486319/1‑101 (MQ=14)
                                                                                                    cATCCTTTCACCTTGCCTTAAATATCCCGGGGGGTGAACTGGCC....GAAGACCAAGAGGGGGGCAGCGCCCCTTTTTCGGGGGGGGCCGCAGGGCCGCACCCt     <  2:2638958/101‑1 (MQ=9)
                                                                                                    cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt     >  2:263089/1‑101 (MQ=14)
                                                                                                    cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt     <  1:549071/101‑1 (MQ=14)
                                                                                                      tCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGt   >  2:1007778/1‑101 (MQ=14)
                                                                                                      tCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGt   >  1:453942/1‑101 (MQ=14)
                                                                                                       ccTTTCGCCTGGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTc  <  2:4102175/101‑1 (MQ=9)
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CCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....G....CCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTC  >  NC_014034/1303780‑1303971

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG