Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,885 C→T 3.0% intergenic (+72/+85) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,8850C→T 3.0% 3.2 ‑3.1 ‑0.4132intergenic (+72/+85)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (70/58):  total (70/62)
Fisher's exact test strand distribution p-value = 4.62e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.83e-01

Only 100 of 150 total aligned reads displayed.

ACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....G....CCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAT  >  NC_014034/1303787‑1303978
                                                                                                       |                                                                                                        
aCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCAc                                                                                                          <  2:1520131/101‑1 (MQ=37)
aCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCAc                                                                                                          <  1:4067251/101‑1 (MQ=37)
  ccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCt                                                                                                        <  2:1309633/101‑1 (MQ=37)
   cTGCGCGCCGTGGTCAGCGTTTAGGCGAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCtt                                                                                                       <  1:2694111/101‑1 (MQ=25)
    tGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTg                                                                                                      <  1:1029661/101‑1 (MQ=37)
     gcgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGc                                                                                                     <  2:232175/101‑1 (MQ=37)
     gcgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGc                                                                                                     >  1:4069879/1‑101 (MQ=37)
      cgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGcc                                                                                                    >  2:268817/1‑101 (MQ=37)
      cgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGcc                                                                                                    <  2:709830/101‑1 (MQ=37)
       gcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCg                                                                                                   >  2:198702/1‑101 (MQ=37)
        cgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGc                                                                                                  <  1:2484888/101‑1 (MQ=37)
          ccGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAA.CCCATCCTTTCCCCTTGCCGCaa                                                                                                >  1:2350921/1‑101 (MQ=25)
           cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCaaa                                                                                               <  1:2567733/101‑1 (MQ=37)
           cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCaaa                                                                                               <  1:3252659/101‑1 (MQ=37)
            gTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAat                                                                                              <  1:548970/101‑1 (MQ=37)
            gTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGAAAat                                                                                              <  2:603395/101‑1 (MQ=25)
              ggTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCTCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAatat                                                                                            <  1:4408920/101‑1 (MQ=37)
              ggTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAatat                                                                                            >  1:1970261/1‑101 (MQ=37)
               gTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATc                                                                                           <  2:2894150/101‑1 (MQ=37)
               gTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATc                                                                                           <  2:4366074/101‑1 (MQ=37)
                tCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATcc                                                                                          <  2:3109187/101‑1 (MQ=37)
                  aGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCg                                                                                        <  2:2251175/101‑1 (MQ=37)
                   gCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCCCCCCCGGGGTATTTGGGGCAAGAT.AAAC.CCCCTCCTTTCACCTTGCCGCAAATATCCCgg                                                                                       >  2:4200577/1‑101 (MQ=25)
                    cGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                                      >  2:3450970/1‑101 (MQ=25)
                    cGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                                      <  2:1935375/101‑1 (MQ=37)
                    cGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGAGATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                                      >  1:3764368/1‑101 (MQ=37)
                     gTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggg                                                                                     >  2:1044292/1‑101 (MQ=37)
                       ttAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggggg                                                                                   >  2:3877198/1‑101 (MQ=25)
                        tAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGt                                                                                  <  1:3450426/101‑1 (MQ=37)
                         cggacggggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAAt                                                                              <  1:3652129/95‑1 (MQ=2)
                         aGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCCCCTTGCCGCAAATATCCCGGGGGGTg                                                                                 <  2:2817443/101‑1 (MQ=25)
                         aGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg                                                                                 >  1:2803911/1‑101 (MQ=37)
                          ggCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGa                                                                                <  2:433091/101‑1 (MQ=37)
                          ggCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGa                                                                                <  1:94295/101‑1 (MQ=37)
                           gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                                               <  1:2713358/101‑1 (MQ=37)
                           gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                                               <  2:2298036/101‑1 (MQ=37)
                           gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                                               >  2:627859/1‑101 (MQ=25)
                           gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGaa                                                                               >  1:3093844/1‑101 (MQ=25)
                            cAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGGCAGAT.AAAC.CCCATACTTTCACCTTGCCGCAAATATTCCGGGGGGGGact                                                                              >  2:4417341/1‑99 (MQ=21)
                            cAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt                                                                              <  1:2281954/101‑1 (MQ=37)
                            cAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt                                                                              <  2:3835025/101‑1 (MQ=37)
                            acggggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGG.GGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTgg                                                                           <  2:3002218/98‑1 (MQ=2)
                             aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                             <  2:537103/101‑1 (MQ=37)
                             aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                             <  1:3714195/101‑1 (MQ=37)
                             aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAAtt                                                                             >  1:953618/1‑101 (MQ=25)
                             aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAAC.CCCATACCTGTTCCTTGCCACAAATATGCCgggggggcaact                                                                             >  1:3837157/1‑95 (MQ=12)
                             aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCACCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                             <  1:1772363/101‑1 (MQ=25)
                              aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                                            <  2:2710675/101‑1 (MQ=37)
                              aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                                            <  2:3421725/101‑1 (MQ=37)
                              aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAGTg                                                                            >  2:3748250/1‑101 (MQ=25)
                               gggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGG.GGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgcca                                                                        <  2:4436892/101‑2 (MQ=2)
                               gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                                           <  2:337839/101‑1 (MQ=37)
                               gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                                           >  2:3503051/1‑101 (MQ=37)
                               gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAAGATCCCGGGGGGTGAAtgag                                                                           >  2:2092892/1‑98 (MQ=25)
                               gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                                           <  1:3824735/101‑1 (MQ=25)
                                ggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgc                                                                          >  2:2568582/1‑101 (MQ=37)
                                 gggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgcc                                                                         <  2:344965/101‑1 (MQ=37)
                                  ggCGCTG.CCCCCGCGGCTTCGCGACACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTGGCCGCAAATGTCCCGGGGGGTGAATTGgcc....g                                                                    <  2:4177995/101‑1 (MQ=25)
                                                                        tttGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcg                               <  1:954819/101‑1 (MQ=14)
                                                                          tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                             <  2:3221761/101‑1 (MQ=14)
                                                                          tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                             >  1:3138648/1‑101 (MQ=14)
                                                                          tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                             >  2:3787186/1‑101 (MQ=14)
                                                                          tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                             >  2:2885445/1‑101 (MQ=14)
                                                                          tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                             >  2:3016145/1‑101 (MQ=9)
                                                                            gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                           <  2:4133103/101‑1 (MQ=14)
                                                                            gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                           >  2:806173/1‑101 (MQ=14)
                                                                            gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                           >  2:4154139/1‑101 (MQ=14)
                                                                            gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                           >  1:3736375/1‑101 (MQ=14)
                                                                             ggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccg                          >  2:3622874/1‑101 (MQ=14)
                                                                               cAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca                        >  2:1556678/1‑101 (MQ=14)
                                                                               cAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca                        >  1:3356076/1‑101 (MQ=14)
                                                                                aaGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcag                       >  1:210835/1‑101 (MQ=14)
                                                                                 aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                      <  1:203533/101‑1 (MQ=14)
                                                                                 aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                      >  2:2472281/1‑101 (MQ=14)
                                                                                 aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                      >  2:1255868/1‑101 (MQ=14)
                                                                                 aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                      >  1:2649734/1‑101 (MQ=14)
                                                                                   aT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggc                    >  1:93677/1‑101 (MQ=14)
                                                                                    t.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc                   >  2:4247280/1‑101 (MQ=14)
                                                                                       aaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc                 >  2:1781408/1‑101 (MQ=14)
                                                                                       aaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc                 >  1:3966317/1‑101 (MQ=14)
                                                                                         c.cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc               >  1:1227406/1‑101 (MQ=14)
                                                                                           cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAcc              >  1:2702403/1‑101 (MQ=14)
                                                                                            ccATCCTGTCACCTGGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTGCGGGGCGGGCCGCAGGGCCGCAccc             <  1:4417613/101‑1 (MQ=9)
                                                                                             cctCCTTTCACCTTGCCGCAAATTTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt            <  2:4031089/99‑1 (MQ=9)
                                                                                             cATCCTTTCACCTTGCCTTAAATATCCCGGGGGGTGAACTGGCC....GAAGACCAAGAGGGGGGCAGCGCCCCTTTTTCGGGGGGGGCCGCAGGGCCGCACCCt            <  2:2638958/101‑1 (MQ=9)
                                                                                             cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt            <  1:549071/101‑1 (MQ=14)
                                                                                               tCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGt          >  1:453942/1‑101 (MQ=14)
                                                                                                ccTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTc         >  1:2939892/1‑101 (MQ=14)
                                                                                                 cTTTCACCTTGCCGCAAATGTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa        <  2:4261764/101‑1 (MQ=9)
                                                                                                 cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa        >  2:4376887/1‑101 (MQ=14)
                                                                                                 cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa        >  1:288626/1‑101 (MQ=14)
                                                                                                   ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc      >  1:1608606/1‑101 (MQ=14)
                                                                                                   ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc      >  2:1114489/1‑101 (MQ=14)
                                                                                                   ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc      >  2:2490301/1‑101 (MQ=14)
                                                                                                      aCCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCa   >  1:4357470/1‑101 (MQ=17)
                                                                                                       ccTTGCGGCAAATGTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt  <  2:3837152/101‑1 (MQ=12)
                                                                                                       ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt  >  1:4227772/1‑101 (MQ=17)
                                                                                                       ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt  <  1:3936863/101‑1 (MQ=17)
                                                                                                       ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt  <  1:2706238/101‑1 (MQ=17)
                                                                                                       ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt  <  1:4175414/101‑1 (MQ=17)
                                                                                                       |                                                                                                        
ACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....G....CCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAT  >  NC_014034/1303787‑1303978

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG