Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,892 G→C 3.2% intergenic (+79/+78) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,8920G→C 3.2% 2.5 ‑1.7 ‑0.5133intergenic (+79/+78)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (71/57):  total (72/61)
Fisher's exact test strand distribution p-value = 1.79e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.14e-01

Only 100 of 146 total aligned reads displayed.

GCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....G....CCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAA  >  NC_014034/1303794‑1303984
                                                                                                       |                                                                                                       
gcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCg                                                                                                         >  1:381672/1‑101 (MQ=37)
gcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCg                                                                                                         >  2:198702/1‑101 (MQ=37)
 cgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGc                                                                                                        <  1:2484888/101‑1 (MQ=37)
   ccGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAA.CCCATCCTTTCCCCTTGCCGCaa                                                                                                      >  1:2350921/1‑101 (MQ=25)
    cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCaaa                                                                                                     <  1:3252659/101‑1 (MQ=37)
    cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCaaa                                                                                                     <  1:2567733/101‑1 (MQ=37)
    cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCaaa                                                                                                     >  2:676889/1‑101 (MQ=37)
     gTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAat                                                                                                    >  1:945980/1‑101 (MQ=25)
     gTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGAAAat                                                                                                    <  2:603395/101‑1 (MQ=25)
       ggTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCTCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAatat                                                                                                  <  1:4408920/101‑1 (MQ=37)
        gTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATc                                                                                                 <  2:2894150/101‑1 (MQ=37)
         tCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATcc                                                                                                <  2:3109187/101‑1 (MQ=37)
          cAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCCCAAATATccc                                                                                               >  1:4031346/1‑101 (MQ=25)
            gCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCCCCCCCGGGGTATTTGGGGCAAGAT.AAAC.CCCCTCCTTTCACCTTGCCGCAAATATCCCgg                                                                                             >  2:4200577/1‑101 (MQ=25)
             cGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                                            >  2:3450970/1‑101 (MQ=25)
             cGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGAGATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                                            >  1:3764368/1‑101 (MQ=37)
              gTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggg                                                                                           <  1:2713752/101‑1 (MQ=37)
               tttAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggggg                                                                                          <  2:2141317/101‑1 (MQ=37)
               tttAGGCAAGGGGGCGATG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggggg                                                                                          >  1:4411521/1‑101 (MQ=25)
                ttAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggggg                                                                                         >  2:3877198/1‑101 (MQ=25)
                 tAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGt                                                                                        <  1:3450426/101‑1 (MQ=37)
                  aGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCCCCTTGCCGCAAATATCCCGGGGGGTg                                                                                       <  2:2817443/101‑1 (MQ=25)
                  aGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg                                                                                       >  1:2803911/1‑101 (MQ=37)
                   ggCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGa                                                                                      <  1:94295/101‑1 (MQ=37)
                   ggCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGa                                                                                      <  2:433091/101‑1 (MQ=37)
                    gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                                                     <  2:2298036/101‑1 (MQ=37)
                    gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                                                     <  1:2713358/101‑1 (MQ=37)
                    gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGaa                                                                                     >  1:3093844/1‑101 (MQ=25)
                     acggggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGG.GGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTgg                                                                                 <  2:3002218/98‑1 (MQ=2)
                      ctgggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                                   <  1:2093029/99‑1 (MQ=25)
                      aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCGCCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                                   <  1:2705808/101‑1 (MQ=25)
                      aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                                   <  1:3714195/101‑1 (MQ=37)
                      aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAAC.CCCATACCTGTTCCTTGCCACAAATATGCCgggggggcaact                                                                                   >  1:3837157/1‑95 (MQ=12)
                      aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCACCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                                   <  1:1772363/101‑1 (MQ=25)
                       aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                                                  <  2:3421725/101‑1 (MQ=37)
                        gggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGG.GGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgcca                                                                              <  2:4436892/101‑2 (MQ=2)
                        gggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgcca                                                                              <  2:4155742/101‑2 (MQ=2)
                        gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                                                 <  2:337839/101‑1 (MQ=37)
                          gggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgcc                                                                               <  2:344965/101‑1 (MQ=37)
                                                                 tttGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcg                                     >  1:3534568/1‑101 (MQ=14)
                                                                 tttGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcg                                     <  1:954819/101‑1 (MQ=14)
                                                                  ttGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcgg                                    <  2:4304026/101‑1 (MQ=14)
                                                                   tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                   <  2:1970132/101‑1 (MQ=14)
                                                                   tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                   >  2:2885445/1‑101 (MQ=14)
                                                                   tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                   >  2:3787186/1‑101 (MQ=14)
                                                                   tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                   <  2:3221761/101‑1 (MQ=14)
                                                                   tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                   >  1:3138648/1‑101 (MQ=14)
                                                                   tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                   >  2:3016145/1‑101 (MQ=9)
                                                                     gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                                 >  2:4154139/1‑101 (MQ=14)
                                                                      ggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccg                                >  2:3622874/1‑101 (MQ=14)
                                                                        cAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca                              >  2:1556678/1‑101 (MQ=14)
                                                                        cAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca                              >  1:3356076/1‑101 (MQ=14)
                                                                         aaGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcag                             >  1:777618/1‑101 (MQ=14)
                                                                         aaGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcag                             >  1:210835/1‑101 (MQ=14)
                                                                          aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                            >  2:1255868/1‑101 (MQ=14)
                                                                          aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                            <  1:203533/101‑1 (MQ=14)
                                                                          aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                            >  1:2649734/1‑101 (MQ=14)
                                                                           gAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcaggg                           >  2:4423962/1‑101 (MQ=14)
                                                                             t.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc                         >  2:4247280/1‑101 (MQ=14)
                                                                             t.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc                         <  2:2480909/101‑1 (MQ=14)
                                                                               aaaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccggagggccc                        >  2:2690958/1‑100 (MQ=9)
                                                                               aaaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg                        >  1:1427834/1‑101 (MQ=14)
                                                                               aaaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg                        >  2:423736/1‑101 (MQ=14)
                                                                               aaaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg                        >  2:560068/1‑101 (MQ=14)
                                                                                aaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc                       >  2:1781408/1‑101 (MQ=14)
                                                                                aaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc                       >  1:3966317/1‑101 (MQ=14)
                                                                                 aC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgca                      >  1:3863071/1‑101 (MQ=14)
                                                                                  c.cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc                     <  2:3813664/101‑1 (MQ=14)
                                                                                  c.cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc                     >  2:2303029/1‑101 (MQ=14)
                                                                                  c.cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc                     >  1:1227406/1‑101 (MQ=14)
                                                                                    cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAcc                    >  1:486319/1‑101 (MQ=14)
                                                                                    cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAcc                    >  1:2702403/1‑101 (MQ=14)
                                                                                      cctCCTTTCACCTTGCCGCAAATTTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt                  <  2:4031089/99‑1 (MQ=9)
                                                                                      cATCCTTTCACCTTGCCTTAAATATCCCGGGGGGTGAACTGGCC....GAAGACCAAGAGGGGGGCAGCGCCCCTTTTTCGGGGGGGGCCGCAGGGCCGCACCCt                  <  2:2638958/101‑1 (MQ=9)
                                                                                      cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt                  >  2:263089/1‑101 (MQ=14)
                                                                                      cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt                  <  1:549071/101‑1 (MQ=14)
                                                                                        tCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGt                >  2:1007778/1‑101 (MQ=14)
                                                                                        tCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGt                >  1:453942/1‑101 (MQ=14)
                                                                                         ccTTTCGCCTGGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTc               <  2:4102175/101‑1 (MQ=9)
                                                                                         ccTTTCACCATGCCGCAAATATCCCGGGGGGTGAATTAGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGACGCCGGGCCGCCCCCTGTc               >  2:437568/1‑101 (MQ=2)
                                                                                          cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa              >  2:4376887/1‑101 (MQ=14)
                                                                                          cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa              <  2:1670313/101‑1 (MQ=14)
                                                                                          cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa              >  1:288626/1‑101 (MQ=14)
                                                                                            ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc            >  2:2490301/1‑101 (MQ=14)
                                                                                            ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc            >  2:1114489/1‑101 (MQ=14)
                                                                                            tcccccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc            <  2:3460298/97‑1 (MQ=9)
                                                                                             tCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcg           <  1:3716282/101‑1 (MQ=14)
                                                                                              cACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcgc          <  2:3630601/101‑1 (MQ=17)
                                                                                               aCCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCa         >  1:4357470/1‑101 (MQ=17)
                                                                                                ccTTGCGGCAAATGTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt        <  2:3837152/101‑1 (MQ=12)
                                                                                                ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt        <  1:1962743/101‑1 (MQ=17)
                                                                                                ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt        <  1:4175414/101‑1 (MQ=17)
                                                                                                ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt        >  1:4227772/1‑101 (MQ=17)
                                                                                                 cTTGCCGCAAATCTCGCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATc       <  1:2009981/101‑1 (MQ=12)
                                                                                                 cTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCCGCGCCTc       >  2:665847/1‑101 (MQ=9)
                                                                                                 cTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATc       >  1:1216211/1‑101 (MQ=17)
                                                                                                  ttGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcc      >  2:2808500/1‑101 (MQ=17)
                                                                                                  ttGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcc      <  2:2193020/101‑1 (MQ=17)
                                                                                                   tGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATccc     >  1:300442/1‑101 (MQ=17)
                                                                                                      cGCAAATATCCCGGGGGGTGAATTGGGCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa  <  1:2639677/101‑1 (MQ=12)
                                                                                                       |                                                                                                       
GCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....G....CCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAA  >  NC_014034/1303794‑1303984

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG