Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,303,926	T→G	100%	intergenic (+113/+44)	urtE → / ← RCAP_rcc01231	urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,303,926	0	T→G	100.0%	191.1	92	intergenic (+113/+44)	urtE/RCAP_rcc01231	urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (65/27):  ref base (0/0):  total (65/27)

TTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....G....CCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCAT  >  NC_014034/1303856‑1303996                                                                               |                                                                          tttGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcg                                                 <  1:954819/101‑1 (MQ=14) tttGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcg                                                 >  1:3534568/1‑101 (MQ=14)  ttGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcgg                                                <  2:4304026/101‑1 (MQ=14)   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGTGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                               >  2:300071/1‑101 (MQ=9)   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                               >  2:3787186/1‑101 (MQ=14)   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                               <  2:3221761/101‑1 (MQ=14)   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                               >  2:2885445/1‑101 (MQ=14)   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                               <  2:1970132/101‑1 (MQ=14)   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                               >  1:3138648/1‑101 (MQ=14)   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                               >  2:3016145/1‑101 (MQ=9)     gggCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                                             <  2:4133103/101‑1 (MQ=14)     gggCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                                             >  2:4154139/1‑101 (MQ=14)     gggCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                                             >  1:3736375/1‑101 (MQ=14)     gggCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                                             >  2:806173/1‑101 (MQ=14)      ggCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccg                                            >  2:3622874/1‑101 (MQ=14)        cAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca                                          >  2:3888972/1‑101 (MQ=14)        cAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca                                          >  2:1556678/1‑101 (MQ=14)        cAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca                                          >  1:3356076/1‑101 (MQ=14)         aaGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcag                                         >  1:777618/1‑101 (MQ=14)         aaGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcag                                         >  1:210835/1‑101 (MQ=14)          aGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                                        >  2:3254270/1‑101 (MQ=14)          aGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                                        >  2:2472281/1‑101 (MQ=14)          aGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                                        >  2:1255868/1‑101 (MQ=14)          aGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                                        <  1:203533/101‑1 (MQ=14)          aGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                                        >  1:2649734/1‑101 (MQ=14)           gATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcaggg                                       >  2:4423962/1‑101 (MQ=14)            aTAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggc                                      >  1:93677/1‑101 (MQ=14)             tAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc                                     <  2:2480909/101‑1 (MQ=14)             tAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc                                     >  2:4247280/1‑101 (MQ=14)              aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccggagggccc                                    >  2:2690958/1‑100 (MQ=9)              aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg                                    >  1:1427834/1‑101 (MQ=14)              aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg                                    >  2:423736/1‑101 (MQ=14)              aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg                                    >  2:560068/1‑101 (MQ=14)               aaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc                                   >  1:3966317/1‑101 (MQ=14)               aaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc                                   >  2:1781408/1‑101 (MQ=14)                aCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgca                                  >  1:3863071/1‑101 (MQ=14)                 ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc                                 >  2:2303029/1‑101 (MQ=14)                 ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc                                 <  2:3813664/101‑1 (MQ=14)                 ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc                                 >  1:1227406/1‑101 (MQ=14)                  cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAcc                                >  1:486319/1‑101 (MQ=14)                  cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAcc                                >  1:2702403/1‑101 (MQ=14)                   ccATCCTGTCACCTGGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTGCGGGGCGGGCCGCAGGGCCGCAccc                               <  1:4417613/101‑1 (MQ=9)                    cctCCTTTCACCTTGCCGCAAATTTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt                              <  2:4031089/99‑1 (MQ=9)                    cATCCTTTCACCTTGCCTTAAATATCCCGGGGGGTGAACTGGCC....GAAGACCAAGAGGGGGGCAGCGCCCCTTTTTCGGGGGGGGCCGCAGGGCCGCACCCt                              <  2:2638958/101‑1 (MQ=9)                    cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt                              <  1:549071/101‑1 (MQ=14)                    cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt                              >  2:263089/1‑101 (MQ=14)                      tCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGt                            >  1:453942/1‑101 (MQ=14)                      tCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGt                            >  2:1007778/1‑101 (MQ=14)                       ccTTTCGCCTGGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTc                           <  2:4102175/101‑1 (MQ=9)                       ccTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTc                           >  1:2939892/1‑101 (MQ=14)                       ccTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTc                           >  2:2882358/1‑101 (MQ=14)                       ccTTTCACCATGCCGCAAATATCCCGGGGGGTGAATTAGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGACGCCGGGCCGCCCCCTGTc                           >  2:437568/1‑101 (MQ=2)                        cTTTCACCTTGCCGCAAATGTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa                          <  2:4261764/101‑1 (MQ=9)                        cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa                          <  2:1670313/101‑1 (MQ=14)                        cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa                          >  1:288626/1‑101 (MQ=14)                        cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa                          >  2:4376887/1‑101 (MQ=14)                          ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc                        >  2:2490301/1‑101 (MQ=14)                          ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc                        >  2:1114489/1‑101 (MQ=14)                          ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc                        >  1:1608606/1‑101 (MQ=14)                          tcccccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc                        <  2:3460298/97‑1 (MQ=9)                           tCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcg                       >  1:2494327/1‑101 (MQ=14)                           tCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcg                       <  1:3716282/101‑1 (MQ=14)                            cACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcgc                      <  2:3630601/101‑1 (MQ=17)                             aCCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCa                     >  1:4357470/1‑101 (MQ=17)                              ccTTGCGGCAAATGTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                    <  2:3837152/101‑1 (MQ=12)                              ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                    >  1:4227772/1‑101 (MQ=17)                              ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                    <  1:4175414/101‑1 (MQ=17)                              ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                    <  1:3936863/101‑1 (MQ=17)                              ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                    <  1:1962743/101‑1 (MQ=17)                              ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                    <  1:2706238/101‑1 (MQ=17)                               cTTGCCGCAAATCTCGCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATc                   <  1:2009981/101‑1 (MQ=12)                               cTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCCGCGCCTc                   >  2:665847/1‑101 (MQ=9)                               cTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATc                   >  1:1216211/1‑101 (MQ=17)                               cTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATc                   >  1:2659833/1‑101 (MQ=17)                                ttGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcc                  >  2:2808500/1‑101 (MQ=17)                                ttGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcc                  <  2:2193020/101‑1 (MQ=17)                                 tGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATccc                 >  1:300442/1‑101 (MQ=17)                                 tGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATccc                 >  1:2732783/1‑101 (MQ=17)                                    cGCAAATATCCCGGGGGGTGAATTGGGCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa              <  1:2639677/101‑1 (MQ=12)                                    cGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa              >  1:2053348/1‑101 (MQ=17)                                      cAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgc            >  1:667094/1‑101 (MQ=17)                                      cAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGAAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgc            >  1:3807763/1‑101 (MQ=17)                                       aaaTATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcc           >  2:3635357/1‑101 (MQ=17)                                       aaaTATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcc           >  2:833017/1‑101 (MQ=17)                                       aaaTATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcc           >  1:1603506/1‑101 (MQ=17)                                        aaTCTCCCGGGGTGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCCCCCTGTCAGCGCATCCCCAAgccc          <  2:667874/101‑1 (MQ=12)                                        aaTATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgccc          >  2:907262/1‑101 (MQ=17)                                        aaTATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgccc          >  1:520147/1‑101 (MQ=17)                                         atatCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccg         >  2:4287746/1‑101 (MQ=17)                                           atCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAACCCCCAgcccgcc       >  1:3085756/1‑101 (MQ=12)                                            tCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgccc      >  1:2698747/1‑101 (MQ=18)                                            tCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgccc      >  1:85357/1‑101 (MQ=18)                                             cccGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGcccgcccg     >  1:2348261/1‑101 (MQ=18)                                                ggggggTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCAt  <  2:3093620/101‑1 (MQ=18)                                                                               |                                                                          TTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....G....CCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCAT  >  NC_014034/1303856‑1303996 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG