Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,316,044 A→T 100% K42M (AAG→ATG)  potA1 → polyamine ABC transporter ATP binding protein PotA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,316,0440A→T100.0% 831.4 216K42M (AAG→ATG) potA1polyamine ABC transporter ATP binding protein PotA
Reads supporting (aligned to +/- strand):  new base (121/93):  ref base (1/1):  total (122/94)

Only 100 of 218 total aligned reads displayed.

ATTCGTCGTCTTCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.AGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCGATGGCACCAA  >  NC_014034/1315946‑1316140
                                                                                                   |                                                                                                
aTTCGTCGTCTTCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGc                                                                                                <  2:3489758/101‑1 (MQ=255)
aTTCGTCGTCTTCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGc                                                                                                >  1:2591394/1‑101 (MQ=255)
 ttCGTCGTCTTCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCt                                                                                               >  1:2884949/1‑101 (MQ=255)
    gtcgtcTTCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCgg                                                                                            >  1:310478/1‑101 (MQ=255)
      cgtcTTCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCggcc                                                                                          >  1:993885/1‑99 (MQ=255)
      cgtcTTCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGAc                                                                                          <  2:3624590/101‑1 (MQ=255)
       gtcTTCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGAcc                                                                                         <  2:3727890/101‑1 (MQ=255)
        tcTTCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCt                                                                                        >  1:3469833/1‑101 (MQ=255)
        tcTTCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCt                                                                                        >  1:1703612/1‑101 (MQ=255)
         cTTCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCATAGATCGGaaga                                                                                        >  2:3348533/1‑98 (MQ=255)
         cTTCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCtt                                                                                       >  1:700273/1‑101 (MQ=255)
         cTTCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCtt                                                                                       <  2:2584615/101‑1 (MQ=255)
           tCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCg                                                                                     >  1:1805322/1‑101 (MQ=255)
            cGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCgg                                                                                    >  1:4182281/1‑101 (MQ=255)
            cGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCgg                                                                                    >  1:4350294/1‑101 (MQ=255)
            cGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCgg                                                                                    <  2:1655395/101‑1 (MQ=255)
               ccATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCt                                                                                 >  2:1871919/1‑101 (MQ=255)
                cATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTc                                                                                >  1:3522734/1‑101 (MQ=255)
                 aTGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTcc                                                                               >  2:2181043/1‑101 (MQ=255)
                 aTGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTcc                                                                               >  1:2055561/1‑101 (MQ=255)
                 aTGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTcc                                                                               >  2:2540195/1‑101 (MQ=255)
                 aTGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTcc                                                                               >  2:4198910/1‑101 (MQ=255)
                 aTGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTcc                                                                               <  1:3861634/101‑1 (MQ=255)
                   gtgCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCgg                                                                             <  1:75435/101‑1 (MQ=255)
                    tgCAGAAAAGCTATGACGAGGAGAAGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGACGAATTTCTGAACA.TGCTCGGACCTTCGGGCTCCGGc                                                                            <  2:401481/101‑1 (MQ=255)
                     gCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCa                                                                           <  1:2578247/101‑1 (MQ=255)
                     gCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCa                                                                           >  2:3562941/1‑101 (MQ=255)
                        gAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGa                                                                        <  2:1095734/101‑1 (MQ=255)
                        gAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGa                                                                        >  1:1192629/1‑101 (MQ=255)
                        gAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGa                                                                        >  1:2810317/1‑101 (MQ=255)
                        gAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGa                                                                        <  1:1018173/101‑1 (MQ=255)
                         aaaaGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGac                                                                       >  2:755344/1‑101 (MQ=255)
                            aGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGaccac                                                                    <  1:1663857/101‑1 (MQ=255)
                             gCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGaccacc                                                                   >  2:102515/1‑101 (MQ=255)
                             gCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGaccacc                                                                   >  1:2388414/1‑101 (MQ=255)
                              cTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCAcct                                                                  >  2:3063087/1‑101 (MQ=255)
                              cTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCAcct                                                                  <  1:544036/101‑1 (MQ=255)
                               tATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGATCCGGCACGACCAcctg                                                                 >  2:699442/1‑101 (MQ=255)
                                aTGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCAcctgc                                                                >  1:3035596/1‑101 (MQ=255)
                                aTGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCAcctgc                                                                <  1:3611951/101‑1 (MQ=255)
                                aTGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCAcctgc                                                                >  1:1524695/1‑101 (MQ=255)
                                 tGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCAcctgcc                                                               <  1:1121086/101‑1 (MQ=255)
                                  nnnnnnnnnncGCTCGTNGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCAcctgcct                                                              <  2:529844/91‑1 (MQ=255)
                                  gACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCAcctgcct                                                              >  2:2616081/1‑101 (MQ=255)
                                   aCGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCAcctgcctg                                                             >  2:4144630/1‑101 (MQ=255)
                                    cGGGGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCtga                                                            <  1:846424/101‑1 (MQ=255)
                                    cGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCtga                                                            <  2:4392174/101‑1 (MQ=255)
                                    cGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCtga                                                            <  1:4089944/101‑1 (MQ=255)
                                        gagaCGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTgatgat                                                        >  1:2712509/1‑101 (MQ=255)
                                            cGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCtt                                                    <  1:1993486/101‑1 (MQ=255)
                                             gCTCGTCGTCAAAGCCCTGAACCTGTCGAGTGCGAAGTGCGAATTTCTGCCCA.TGCTCGGGCGTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTg                                                   <  1:2676247/101‑1 (MQ=255)
                                             gCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTTTGACCA.TGCTCGGACCTTCGCGCTCCGGCAAGACCACCTGCCTGATGATGCTTg                                                   >  1:139054/1‑101 (MQ=255)
                                             gCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTg                                                   <  1:1240746/101‑1 (MQ=255)
                                             gCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTg                                                   >  1:1608619/1‑101 (MQ=255)
                                             gCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTg                                                   >  2:3919891/1‑101 (MQ=255)
                                             gCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTAGGGCCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTg                                                   <  2:3004283/101‑1 (MQ=255)
                                              cTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGc                                                  <  2:2311570/101‑1 (MQ=255)
                                               tcgtcgTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCg                                                 <  1:490250/101‑1 (MQ=255)
                                                 gtcgtcAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCggg                                               >  2:2067995/1‑101 (MQ=255)
                                                    gtcAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCtt                                            >  1:2369812/1‑101 (MQ=255)
                                                     tcAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTc                                           <  2:1976013/101‑1 (MQ=255)
                                                       aaaGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCga                                         >  1:4333168/1‑101 (MQ=255)
                                                        aaGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGTCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCgag                                        >  1:3804544/1‑101 (MQ=255)
                                                         aGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGGCCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCgaga                                       <  1:3370193/101‑1 (MQ=255)
                                                          gACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGAc                                      <  2:3238485/101‑1 (MQ=255)
                                                          gACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGAc                                      <  1:2873053/101‑1 (MQ=255)
                                                           aCCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACg                                     <  1:905791/101‑1 (MQ=255)
                                                           aCCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACg                                     >  1:782080/1‑101 (MQ=255)
                                                           aCCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACg                                     >  1:3412825/1‑101 (MQ=255)
                                                               gAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGcgc                                 >  1:3123328/1‑100 (MQ=255)
                                                               gAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGcga                                 <  2:2884741/101‑1 (MQ=255)
                                                                aaCCTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGcgac                                >  1:611452/1‑101 (MQ=255)
                                                                  ccTGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGcgacga                              >  1:4379006/1‑101 (MQ=255)
                                                                    tGTCGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAAc                            >  2:1862714/1‑101 (MQ=255)
                                                                       cGATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACCGCGACGAACggg                         >  2:1188419/1‑101 (MQ=255)
                                                                        gATTGCGAAGGGCGAATTTCTTACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGgc                        >  1:3296359/1‑101 (MQ=255)
                                                                        gATTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGgc                        <  2:1486329/101‑1 (MQ=255)
                                                                         aTTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGgcg                       >  2:371138/1‑101 (MQ=255)
                                                                         aTTGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGgcg                       >  1:1070145/1‑101 (MQ=255)
                                                                          ttGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGCGACGGCGACGACAGGgcgc                      >  2:3791084/1‑101 (MQ=255)
                                                                          ttGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGgcgc                      <  1:3651217/101‑1 (MQ=255)
                                                                          ttGCGAAGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGgcgc                      <  1:1126659/101‑1 (MQ=255)
                                                                               aaGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCt                 <  1:3425122/101‑1 (MQ=255)
                                                                                aGGGCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTg                >  1:3698963/1‑101 (MQ=255)
                                                                                  ggCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCt              >  2:1452455/1‑101 (MQ=255)
                                                                                   gCGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTc             >  2:2025493/1‑101 (MQ=255)
                                                                                    cGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCg            >  1:2177016/1‑101 (MQ=255)
                                                                                    cGAATTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCg            <  1:244014/101‑1 (MQ=255)
                                                                                      aaTTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCGAt          >  1:3464311/1‑101 (MQ=255)
                                                                                      aaTTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCGAt          <  1:298435/101‑1 (MQ=255)
                                                                                      aaTTTCCGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCGAt          <  2:611384/101‑1 (MQ=255)
                                                                                       aTTTCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCCTCCTGCTCGATg         >  2:1564766/1‑101 (MQ=255)
                                                                                        tttCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCGATgg        <  1:2269171/101‑1 (MQ=255)
                                                                                        tttCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCGATgg        >  1:1458593/1‑101 (MQ=255)
                                                                                         ttCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCGATGGc       >  2:4304010/1‑101 (MQ=255)
                                                                                          tCTGACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCGATGGCa      <  1:2068132/101‑1 (MQ=255)
                                                                                             gACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCGATGGCACCa   <  1:3920145/101‑1 (MQ=255)
                                                                                             gACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCGATGGCACCa   <  2:1643573/101‑1 (MQ=255)
                                                                                             gACCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCGATGGCACCa   >  2:3396726/1‑101 (MQ=255)
                                                                                              aCCA.TGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCGATGGCACCaa  >  2:2492297/1‑101 (MQ=255)
                                                                                                   |                                                                                                
ATTCGTCGTCTTCGACCATGTGCAGAAAAGCTATGACGGCGAGACGCTCGTCGTCAAAGACCTGAACCTGTCGATTGCGAAGGGCGAATTTCTGACCA.AGCTCGGACCTTCGGGCTCCGGCAAGACCACCTGCCTGATGATGCTTGCGGGCTTCGAGACGGCGACGAACGGGCGCATCCTGCTCGATGGCACCAA  >  NC_014034/1315946‑1316140

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG