Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,325,125 G→T 100% P351T (CCC→ACC)  dacC2 ← D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,325,1250G→T100.0% 584.0 164P351T (CCC→ACC) dacC2D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  new base (81/81):  ref base (2/0):  total (83/81)

Only 100 of 191 total aligned reads displayed.

ACCATCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGGGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCGGCC  >  NC_014034/1325025‑1325222
                                                                                                    |                                                                                                 
  caTCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGCGAAGCGGACCAGGAAGCCGGCCTCGGCCACGTCGGTTTCCGCCACCAGCCGCAGGGTgc                                                                                                 >  2:1096294/1‑101 (MQ=255)
     cTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGca                                                                                              >  2:3494898/1‑101 (MQ=255)
     cTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGca                                                                                              <  1:3982962/101‑1 (MQ=255)
     cTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCCCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGca                                                                                              <  2:719499/101‑1 (MQ=255)
      tGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcag                                                                                             >  2:1945774/1‑101 (MQ=255)
      tGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcag                                                                                             >  2:2298756/1‑101 (MQ=255)
          gggTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcccc                                                                                         <  1:1955688/101‑1 (MQ=255)
          gggTCGGCACCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcccc                                                                                         <  2:1409657/101‑1 (MQ=255)
           ggTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCCCCAGCGGCACGGTGCGcagccccg                                                                                        >  2:1390437/1‑100 (MQ=255)
           ggTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagccccc                                                                                        >  2:2137317/1‑100 (MQ=255)
           ggTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcccca                                                                                        <  1:4088008/101‑1 (MQ=255)
           ggTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcccca                                                                                        <  2:3975699/101‑1 (MQ=255)
            gTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagccccag                                                                                       <  1:2769266/101‑1 (MQ=255)
             tCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagccccagc                                                                                      <  2:2548350/101‑1 (MQ=255)
               tGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagccccagccc                                                                                    <  2:2073593/101‑1 (MQ=255)
                gcgcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCagccccagcccc                                                                                   <  1:598575/101‑1 (MQ=255)
                gcgcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGGGCGCagccccagcccc                                                                                   >  1:3865020/1‑101 (MQ=255)
                  gcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCgg                                                                                 <  2:1229547/101‑1 (MQ=255)
                    cAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCa                                                                               >  2:389162/1‑101 (MQ=255)
                     aGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCAc                                                                              <  2:1645090/101‑1 (MQ=255)
                     aGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGGGCGCAGCCCCAGCCCCGGCAc                                                                              >  2:4272108/1‑101 (MQ=255)
                      gAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCGCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACg                                                                             <  1:4401782/101‑1 (MQ=255)
                         gCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTg                                                                          <  1:1674429/101‑1 (MQ=255)
                           cGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAt                                                                        >  2:2384116/1‑101 (MQ=255)
                            ggCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAtt                                                                       >  2:1277890/1‑101 (MQ=255)
                               ccGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTcc                                                                    >  1:95426/1‑101 (MQ=255)
                                cgcgGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCa                                                                   >  2:428627/1‑101 (MQ=255)
                                 gcggcggcgGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAg                                                                  >  1:829560/1‑101 (MQ=255)
                                  cggcggcggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAgc                                                                 >  2:1256398/1‑101 (MQ=255)
                                    gcggcggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAgccg                                                               >  2:817197/1‑101 (MQ=255)
                                     cggcggTGAAGCGGGCCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAgccgc                                                              <  1:4431045/101‑1 (MQ=255)
                                         ggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCa                                                          <  1:3495138/101‑1 (MQ=255)
                                         ggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCa                                                          >  2:607095/1‑101 (MQ=255)
                                         ggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGCGATTTCCAGCCGCCCCa                                                          >  2:659662/1‑101 (MQ=255)
                                         ggTGAAGCGCACCCGGAAGCCGCCCTCGGCCCCGTCGGTTTCCGCCACCAGCGGCACGGTGCGCGGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCa                                                          <  2:1327712/101‑1 (MQ=255)
                                          gTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAg                                                         <  1:1072247/101‑1 (MQ=255)
                                            gAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCt                                                       <  1:1551595/101‑1 (MQ=255)
                                            gAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCt                                                       <  2:1046151/101‑1 (MQ=255)
                                             aaGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTc                                                      >  1:1316885/1‑101 (MQ=255)
                                             aaGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTc                                                      >  2:110926/1‑101 (MQ=255)
                                             aaGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCACCTc                                                      >  1:726917/1‑101 (MQ=255)
                                              aGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCg                                                     <  2:3581609/101‑1 (MQ=255)
                                              aGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCg                                                     <  2:625082/101‑1 (MQ=255)
                                               gCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCgg                                                    <  2:3923819/101‑1 (MQ=255)
                                                cGGACCAGGAAGCCGCCCTCGGCTACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGt                                                   >  2:948395/1‑101 (MQ=255)
                                                cGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGt                                                   <  2:3864767/101‑1 (MQ=255)
                                                 ggACCTGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAACCCCAGCCCCGGAACGGTGATTTACAGCCGCCCCAGCTCgggg                                                  >  1:301869/1‑99 (MQ=255)
                                                 ggACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTg                                                  >  1:3205565/1‑101 (MQ=255)
                                                  gccagggAGGCCGCCCTCGGCCCCGTCGGTTTCCGCCACCAGCGGCACCGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGc                                                 <  1:2260560/96‑1 (MQ=255)
                                                  gACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGc                                                 >  1:486833/1‑101 (MQ=255)
                                                   aCCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGcc                                                >  2:3747677/1‑101 (MQ=255)
                                                   aCCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGcc                                                >  2:2398246/1‑101 (MQ=255)
                                                    ccAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGccc                                               <  2:1444333/101‑1 (MQ=255)
                                                    ccAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGccc                                               >  1:3378061/1‑101 (MQ=255)
                                                     cAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccg                                              <  2:2443356/101‑1 (MQ=255)
                                                      aGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccgc                                             <  1:1789802/101‑1 (MQ=255)
                                                       ggAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccgac                                            >  2:2194395/1‑99 (MQ=255)
                                                       ggAAGCCGCCCCCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccgcc                                            <  2:1728335/101‑1 (MQ=255)
                                                        gAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccgccg                                           <  1:2194534/101‑1 (MQ=255)
                                                        gAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccgccg                                           >  2:3123604/1‑101 (MQ=255)
                                                        gAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccgccg                                           >  2:2205563/1‑101 (MQ=255)
                                                            ccgccCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccgccgcaag                                       <  2:2011489/101‑5 (MQ=255)
                                                             cgccCTCGGCCCCGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCAcc                                      <  2:301834/101‑1 (MQ=255)
                                                             cccccCCGCCCCCGTCGGTTCCCGCCCCCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTTTTTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCAcc                                      <  1:2384266/99‑1 (MQ=255)
                                                              gccCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCg                                     <  1:914329/101‑1 (MQ=255)
                                                                   cGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGcgcg                                >  1:3504157/1‑101 (MQ=255)
                                                                    ggCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCgg                               <  1:3132175/101‑1 (MQ=255)
                                                                    ggCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCgg                               >  2:4107700/1‑101 (MQ=255)
                                                                     gCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGt                              >  2:2434382/1‑101 (MQ=255)
                                                                     gCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGt                              <  1:3710629/101‑1 (MQ=255)
                                                                     gCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGt                              >  2:345627/1‑101 (MQ=255)
                                                                      ccACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTg                             >  2:3357960/1‑101 (MQ=255)
                                                                      ccACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTg                             <  1:2887731/101‑1 (MQ=255)
                                                                        ccGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGAc                           <  1:1908742/100‑1 (MQ=255)
                                                                        aCGTCGGTTTCCGCCCGCAGCAGCACGGTGCGCAACACCAGCCCCGGCACGGCGGCGTTGAGCCGCCCCATCCCGGGGCCCGCCGCCACCGCcgcgcgggc                           >  2:3074217/1‑96 (MQ=255)
                                                                          gtgggTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCg                         >  1:2557870/4‑101 (MQ=255)
                                                                            cGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCggg                       >  1:808201/1‑101 (MQ=255)
                                                                              gTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGcc                     <  1:3459898/101‑1 (MQ=255)
                                                                              gTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGcc                     <  1:2063467/101‑1 (MQ=255)
                                                                              gTTTCCCCCACCAGCGGCCCGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGcc                     <  1:1236629/101‑1 (MQ=255)
                                                                               tttCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCg                    >  2:562667/1‑101 (MQ=255)
                                                                               tttCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCg                    >  2:2473018/1‑101 (MQ=255)
                                                                               tttCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCg                    >  1:1416414/1‑101 (MQ=255)
                                                                                ttCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGt                   >  2:3661575/1‑101 (MQ=255)
                                                                                   cGCCGCCAGCGGGACGGTGCGCGCCCCCAGCCCCGGCCCGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCaa                <  2:1933927/101‑1 (MQ=255)
                                                                                   cGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCaa                >  2:959223/1‑101 (MQ=255)
                                                                                    ccccccaGCGGCCCGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCaaa               <  1:4245661/97‑1 (MQ=255)
                                                                                     ccaccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAg              >  1:3269673/1‑101 (MQ=255)
                                                                                     ccaccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAg              >  2:161661/1‑101 (MQ=255)
                                                                                      caccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGa             >  2:4419565/1‑101 (MQ=255)
                                                                                      caccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGa             <  1:3698132/101‑1 (MQ=255)
                                                                                         caGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCg          <  2:1702481/101‑1 (MQ=255)
                                                                                          aGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGc         >  1:2802843/1‑101 (MQ=255)
                                                                                            cGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCt       >  2:354842/1‑101 (MQ=255)
                                                                                            cGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCt       <  2:353898/101‑1 (MQ=255)
                                                                                            cGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCt       <  2:3205336/101‑1 (MQ=255)
                                                                                             ggCACGGTGCGCACGCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCGGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTc      <  2:2877906/101‑1 (MQ=255)
                                                                                              gcccGGTGCGCAGCCCCAGCCCCGGCCCGGTGATTCCCGGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCg     <  2:486781/98‑1 (MQ=255)
                                                                                              gCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCg     <  1:4272377/101‑1 (MQ=255)
                                                                                                ccGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCGGc   <  2:4165501/100‑1 (MQ=255)
                                                                                                    |                                                                                                 
ACCATCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGGGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCGGCC  >  NC_014034/1325025‑1325222

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG