Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,329,189 C→G 21.2% intergenic (‑127/+114) RCAP_rcc01255 ← / ← RCAP_rcc01256 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,329,1890C→G21.2% 23.1 ‑2.4 ‑0.2108intergenic (‑127/+114)RCAP_rcc01255/RCAP_rcc01256hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/23):  ref base (20/62):  total (23/85)
Fisher's exact test strand distribution p-value = 9.37e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.84e-01

Only 100 of 188 total aligned reads displayed.

TGATGGGCGCGCAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGGGCGGCTGCAGCCA  >  NC_014034/1329089‑1329289
                                                                                                    |                                                                                                    
   tGGGCGCGCAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGAg                                                                                                   <  1:4133175/101‑1 (MQ=255)
   tGGGCGCGCAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGCCGGGGCGAg                                                                                                   >  2:1256285/1‑101 (MQ=255)
    gggCGCGCAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGAGc                                                                                                  <  1:2436741/101‑1 (MQ=255)
    gggCGCGCAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGCGGGGGGGAGc                                                                                                  >  2:2527510/1‑101 (MQ=255)
          gcagcaGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGCGGGGGCGGGCCggggg                                                                                            >  2:2372631/1‑97 (MQ=255)
           cagcagGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGCGGGGGGGGGcccgggat                                                                                           >  1:4319347/1‑95 (MQ=255)
            agcagGTAGCCTTCCGCGCATCGAATCCCGCCTGCTCCCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTa                                                                                          <  1:3232766/101‑1 (MQ=255)
            agcagGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTTTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGCGGGGGCGGGCCGTGGTTa                                                                                          >  2:1418076/1‑101 (MQ=255)
            agcagGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTa                                                                                          <  2:3260326/101‑1 (MQ=255)
            agcagGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGCGGGGGGGGGCCgggggtt                                                                                          >  1:3137652/1‑95 (MQ=255)
            agcagGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGCGGGGGGGGGCCGGGGTTa                                                                                          >  1:3861778/1‑101 (MQ=255)
             gcagGTAGCCTCCCGCGCATGGAACCCCGCCTGCTCCCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTac                                                                                         <  2:3722067/101‑1 (MQ=255)
              gagGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTaca                                                                                        <  2:2686544/100‑1 (MQ=255)
               agGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGCGGGGGGGGGCCggggtttccc                                                                                       >  1:2100024/1‑92 (MQ=255)
               agGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGCGGGGGGGAGCCGCGATTatac                                                                                       >  2:3448477/1‑101 (MQ=255)
                ggTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCCGGCCGGGGGGAGCCGGGGTTacacc                                                                                      >  2:1676555/1‑100 (MQ=255)
                  tAGCCTTCCGCGCATCGAACCCCGCCTGCTACCTCTCGACGATGGGCAACGGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTg                                                                                    <  2:365549/101‑1 (MQ=255)
                   acccTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTgc                                                                                   <  2:943183/99‑1 (MQ=255)
                    cccTCCCGGGCATCGAACCCCCCCTGCTCCCTCTGGACGAGGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGGGGGGGCGAGCCGTGATTACACGTgcg                                                                                  <  2:3031211/100‑1 (MQ=255)
                     ccTTCCGCGCATCGAATCCCGCCTCCTCCCTCCCGACGAGGGGCAACGGGGGGGTTTTGTGGCTCGCCCCGAGGGGGGGCGAGCCGTTATTACACGTgcgg                                                                                 <  2:592362/101‑1 (MQ=255)
                     ccTTCCGCCCATCAACTCCCGCCTGCTCCCTCTCGACGATGGGCAACAGGGGGGCTTGGTGGCCCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTgcgg                                                                                 <  1:1994645/101‑1 (MQ=255)
                      cTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCGCGCGGGGGGGGGGCGGGGTTTCACGGgcggg                                                                                >  2:1173225/1‑101 (MQ=255)
                       ttCCGCGCATCGAATCCCGCCTGCTCCCTCTCGACGATGGGCAACAGGGGGGCTTTGGGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTgcgggc                                                                               <  2:821418/101‑1 (MQ=255)
                         ccGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGCGGGGGCGGGGCGGGGTTACACGGgcgggggg                                                                             >  2:1603318/1‑101 (MQ=255)
                          cgcgCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGCGGGGGGGGGCCCGGGTTACACATggggggggg                                                                            >  2:1963589/1‑100 (MQ=255)
                          cgcgCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGCGGGGGCGGGGCGGGGTTAAACCTgcggtgggg                                                                            >  1:1704810/1‑96 (MQ=255)
                           gcgcATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCGAGCGGGGGGGAGCCGTGCTTACACGTgcgggggggg                                                                           >  2:1485443/1‑99 (MQ=255)
                           ccccaccGAACCCCCCCTGCCCCCTCCCGAAGAGGGGCAACAGGGGGGCTTTGTGGCCCCCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTg                                                                           <  1:2499472/95‑1 (MQ=255)
                            cgcATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCAGCGGGGGGGGGCCGGGGTTAAACGTgcggggggggg                                                                          >  1:3416165/1‑98 (MQ=255)
                            cccaccgacccccGCCTGCTCCCTCTCGACGATGGGCAACGGGGGGGCTTTGTGGCTCGCCCCGAGGGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGa                                                                          <  1:3086443/91‑1 (MQ=255)
                              cATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGCGCGGGGGCGGGCCGGGGTTAAACGTGCGGGGGGGGagg                                                                        >  2:2362020/1‑99 (MQ=255)
                               aTCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATgg                                                                       <  1:3978414/101‑1 (MQ=255)
                                tcgcgccccGCGTGCTCCCTCCCGACGATGGGCAACGGGGGGGTTTGGTGGCGCCCCCGGGGCGGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATggg                                                                      <  2:3007918/95‑1 (MQ=255)
                                cagAATCCCGCCTGCTACCGCTCGACGATGGGCAACAGGGGGGCTTTGTGGCCCGCCCGGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATggg                                                                      <  2:2603707/99‑1 (MQ=255)
                                 cGAATCCCGCCTGCTACCTCTGGACGATGGGCAACAGGGGGGCTTTGTGGCTTCCCCCCCGCGGGGGGGAGCCGGGAGTACACGTgggggggggggggggg                                                                     >  1:2544246/1‑93 (MQ=255)
                                 cGAACCCCGCCTGCTCCCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGa                                                                     <  1:3212287/101‑1 (MQ=255)
                                  gAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCAGCGGGGGGCGGCCGGGGTTACACGTgcgggggggggggggcg                                                                    >  2:3514492/1‑92 (MQ=255)
                                  gAAACCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCACCGAGCGGGGGCGAGCCGGGATTACCAGTGCGGGCGGTGGTGGGAg                                                                    >  2:3300887/1‑101 (MQ=255)
                                   aaTCCCGCCTGCTAGCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCAGCGGGGGCGGGCCGTGGTTATCAGTGCGGGCGGGGGTGGGGGc                                                                   >  2:2766306/1‑101 (MQ=255)
                                   aaTCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCAGCGGGGGGGAGCCGGGATTAAACGTGGGGGGGGGGATGGGAGc                                                                   >  1:2215942/1‑101 (MQ=255)
                                     tCCCGCCTGCTCCCTTTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGGGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGccc                                                                 <  1:1418183/101‑1 (MQ=255)
                                      cccGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCAGCGGGGGGGGGGCGGGAGTACACGTGCGGGCGGGGGTggggggccc                                                                >  2:3070298/1‑95 (MQ=255)
                                       ccGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGCGCGGGGGGGGGCCGTGATTACACGTGCGGGCGGGGGTgggtggcccg                                                               >  1:733782/1‑94 (MQ=255)
                                        cGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCGCGCGGGGGCGGGGCGCGGTTTCACGTGCGGGCGGTGGTGGGGGCCCCGt                                                              >  2:2877764/1‑101 (MQ=255)
                                         gCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGGGGGGCGGGGTTACACGTGCGGGCGGGGGTGGGGGGCCAggg                                                             >  1:3344789/1‑99 (MQ=255)
                                          ccTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCAGCGGGGGGGAGCCGGGATTTCACGTGCGGGCGGGGGTGGGAGGCCAGTGc                                                            >  1:2164233/1‑101 (MQ=255)
                                           cggCTCCCTCTCGGCGAGGGGAAAAGGGGGGGCTTTGTGGCTCGCCCCGAGGGGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCg                                                           <  1:378848/99‑1 (MQ=255)
                                           cTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCCAGCGGGGGGGAGCCGGGGTTACACGTGGGGGCGGGGGTGGGAGcgccggggg                                                           >  2:1305115/1‑93 (MQ=255)
                                             gCTTCCTCTGGACGATGGGCAACGGGGGGGTTTTGTGGCGCGCCCCGAGCGGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTg                                                         <  2:1511833/101‑1 (MQ=255)
                                              ctcccTCTCGACGAGGGGCAACGGGGGGGTTTTGGGGCTCGCCCGGGGGGGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTgg                                                        <  1:3517632/98‑1 (MQ=255)
                                              cTACCGCTGGACGGTGGGGAACGGGGGGGCTTTGGGGCCCGCCCGGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTgg                                                        <  2:3776631/101‑1 (MQ=255)
                                               tACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCCCGCGGGGGCGAGGCGTGGTTAAACGGGCGGGCGGGGGTGGGGGCCCAGTGCGGggg                                                       >  1:1469309/1‑101 (MQ=255)
                                               tACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCAGCGGGGGGGGGCCGGGGTTACACGGGCGGGGGGGGGGGGGGGCCCCGGGCGGggg                                                       >  1:106718/1‑101 (MQ=255)
                                               tACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGGGATGGGAGCGCACGTCGTggg                                                       >  2:4094272/1‑101 (MQ=255)
                                                  ctctCGCAGGTGGGGCACAGGGGGACTTTTTGGCGGCCCCAGAGGGGAGGCGAGCCGTCATTACACGTGCGGGCGGTGATGGCAGCCCAGTGCGTGGGCGa                                                    <  2:367762/101‑1 (MQ=255)
                                                  ctctCGACGATGGGCAACGGGGGGGCTTTGTGGCTCGCCCGGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGa                                                    <  1:1676666/101‑1 (MQ=255)
                                                    ctcGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCCAGCGGGGGCGAGCCGGGATTACACGTGCGGGCGGGGATGGGGGCCCAGGGCGGGGGGGATg                                                  >  2:2111087/1‑101 (MQ=255)
                                                     ccgaggaTGGGCAACGGGGGGGTTTTGTGCCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATgg                                                 <  2:1708613/100‑1 (MQ=255)
                                                      cgacgaTGGGCAACAGGGGGGCTTTGTGGCTCGCCCCCAGCGGGGGCGAGCCGCGATTATACCTGCGGGCGGGGAGGGGTGGCCAGAGCGCGGGCGGTggg                                                >  2:4427015/1‑100 (MQ=255)
                                                      cgacgaGGGGCAACGGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGga                                                <  2:2944855/101‑1 (MQ=255)
                                                        acgaTGGGCAACGGGGGGGCTTGGGGGCGCGCCCGGGGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGgaga                                              <  2:3950096/101‑1 (MQ=255)
                                                          gaTGGGCAGCGGGGGGTCTTTGGGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGc                                            <  2:1150284/101‑1 (MQ=255)
                                                          gaTGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGGGAGCCGGGATTACACGTGCGGGCGGGGGTGGGAGCCCCGTGCGGGGGCGGTGGGGAGc                                            >  1:1890893/1‑101 (MQ=255)
                                                           aggggCAACGGGGGGGCTTTGGGGCTCGCCCCGAGGGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGcc                                           <  1:585568/99‑1 (MQ=255)
                                                            tGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGGGCCGGGATTACACGTGCGGGCGGGGATGGGAGCCCAGTGCGGGGGCGGTGGGGGGCCg                                          >  1:3586250/1‑101 (MQ=255)
                                                             gggCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGGGCCGCGATTACACGTGGGGGCGGGGGTGGGAGCCCCATGCGTGGGGGATGgaggggcgg                                         >  1:3187118/1‑95 (MQ=255)
                                                              ggcacggggggggTTTGGTGGCTCGCCCCGAGGGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCgtg                                        <  2:1572870/94‑1 (MQ=255)
                                                               gCAACAGGGGGGCTTTGTGGCTCGCCCCCAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGGGATGGGAGGCCAGGGCGTGGGCGAGGGGGAGCCgtgg                                       >  1:1235806/1‑100 (MQ=255)
                                                                   cAGTGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGGGCCCAGTGCGTGGGCGATGGGGGGCCGTGGgggg                                   >  2:3217379/1‑99 (MQ=255)
                                                                   cAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGGGAGCCGGGATTACACGTGCGGGCGGGGATGGGAGGCCAGGGCGTGGGCGATGGAGAGCCGGGTgggg                                   >  1:2920824/1‑99 (MQ=255)
                                                                    gggggggCTTTGTGGCCCGCCCCGGGGGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGc                                  <  1:2685042/100‑1 (MQ=255)
                                                                    aGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGGGGGCCGGGGTTACACGGGCGGGGGGTGATGGGAGCCCCGCGCGCGGGCGATGGAGAGCCGTGTGGGGc                                  >  1:411009/1‑101 (MQ=255)
                                                                       ggggCTTTGGGGCTCGCCCGGAGCGGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATg                               <  2:1890769/101‑1 (MQ=255)
                                                                        gggTTTGGTGCCTCGCCCCGGGGGGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGa                              <  2:2164094/101‑1 (MQ=255)
                                                                           tttggtgGCCCGCCCCGGGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGAccc                           <  1:2256223/97‑1 (MQ=255)
                                                                           cTTTGTGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGGGATGGGAGCCCAGGGCGTGGGGGATGGAGAGCCGTGTGGGGCATGAccc                           >  1:2856946/1‑101 (MQ=255)
                                                                              tggggCTCGCCCCGGGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACccgcc                        <  2:390282/98‑1 (MQ=255)
                                                                               gtgGCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGGGATGGGAGCCCCGTGCGTGGGCGGTGGAGAGCCGTGTGGAGCATGACccgccg                       >  2:4282302/1‑101 (MQ=255)
                                                                               gtgGCTCGCCCCGAGCGGGGGCGAGCCGGGATTACACGTGCGGGCGGGGATGGGAGCCCAGTGCGGGGGCGATGGAGAGCCGTGTGGAGGATGACccgccg                       >  2:3347364/1‑101 (MQ=255)
                                                                               ggggCTCGCCCGGGGGGGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACccgccg                       <  2:4035753/99‑1 (MQ=255)
                                                                                tgGCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCAtagatcggaag                      >  1:1165193/1‑91 (MQ=255)
                                                                                 ggcccccccgGAGGGGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGaa                     <  2:4244676/95‑1 (MQ=255)
                                                                                 ggCTCGCCCCGGGGGGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGaa                     <  1:2601664/101‑1 (MQ=255)
                                                                                 ggCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGaa                     >  1:654925/1‑101 (MQ=255)
                                                                                  gCTCGCCCCGAGCGGGGGAGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCAGTGTGGAGCGTGACCCGCCGAag                    >  1:2963649/1‑101 (MQ=255)
                                                                                   cTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAaga                   >  2:4383952/1‑101 (MQ=255)
                                                                                     cggcgggggggggggCGGGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGGGTGGGGGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgc                 <  1:2772313/91‑1 (MQ=255)
                                                                                     ccccccGGGGGGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgc                 <  1:821508/99‑1 (MQ=255)
                                                                                     cGCCCGGAGGGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgc                 <  1:4427287/101‑1 (MQ=255)
                                                                                     cGCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgc                 >  1:4092554/1‑101 (MQ=255)
                                                                                      gccgggggggggggGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcg                <  1:343225/92‑1 (MQ=255)
                                                                                      gCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcg                >  1:85333/1‑101 (MQ=255)
                                                                                        ccggggggggggCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcggg              <  1:4048225/94‑1 (MQ=255)
                                                                                         gggggggggggGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcgggc             <  1:107079/95‑1 (MQ=255)
                                                                                           gggggggggCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcgggcgg           <  1:1720848/97‑1 (MQ=255)
                                                                                             gggggggGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGGGCGGCt         <  1:4180083/99‑1 (MQ=255)
                                                                                             gggggggGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGGGCGGCt         <  2:3861525/99‑1 (MQ=255)
                                                                                               gggggGGAGCCGTGATTACACGTGGGGGCGGTGAGGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGGGCGGCTGc       <  1:157608/101‑1 (MQ=255)
                                                                                               gggggCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGGGCGGCTGc       >  2:2205821/1‑101 (MQ=255)
                                                                                               gggggCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGGGCGGCTGc       >  2:553768/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TGATGGGCGCGCAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGCGGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGGGCGGCTGCAGCCA  >  NC_014034/1329089‑1329289

Base quality scores:  ATCG  < 3 ≤  ATCG  < 8 ≤  ATCG  < 21 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG