Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,394,037 T→C 100% V59A (GTC→GCC)  RCAP_rcc01305 → Cas1 family CRISPR‑associated protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,394,0370T→C100.0% 795.5 211V59A (GTC→GCC) RCAP_rcc01305Cas1 family CRISPR‑associated protein
Reads supporting (aligned to +/- strand):  new base (111/100):  ref base (0/0):  total (111/100)

Only 100 of 218 total aligned reads displayed.

CGAGATCGAGGGTGCCGACAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGTCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCAATGTGCTGTTGCG  >  NC_014034/1393939‑1394133
                                                                                                  |                                                                                                
 gagaTCGAGGGTGCCGACAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTg                                                                                               >  1:3163555/1‑101 (MQ=255)
  agaTCGAGGGTGCCGACAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTgc                                                                                              <  1:2301632/101‑1 (MQ=255)
  agaTCGAGGGTGCCGACAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTgc                                                                                              <  1:1962330/101‑1 (MQ=255)
   gaTCGAGGGTGCCGACAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTgcg                                                                                             >  2:1874783/1‑101 (MQ=255)
   gaTCGAGGGTGCCGACAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTgcg                                                                                             >  2:3669939/1‑101 (MQ=255)
         gggTGCCGACAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAgc                                                                                       >  2:1024496/1‑101 (MQ=255)
          ggTGCCGACAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAgcg                                                                                      >  2:3559452/1‑101 (MQ=255)
                gACAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCAt                                                                                >  2:2160606/1‑101 (MQ=255)
                 aCAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATc                                                                               <  1:872675/101‑1 (MQ=255)
                 aCAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATc                                                                               >  2:2812552/1‑101 (MQ=255)
                 aCAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATc                                                                               >  1:104872/1‑101 (MQ=255)
                 aCAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATc                                                                               >  2:4257289/1‑101 (MQ=255)
                  cAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCa                                                                              >  1:1144095/1‑101 (MQ=255)
                  cAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCa                                                                              >  1:2704714/1‑101 (MQ=255)
                   aaGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCAc                                                                             >  2:2766413/1‑101 (MQ=255)
                     gACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGCCATCAccc                                                                           >  2:1114680/1‑101 (MQ=255)
                      aCTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCt                                                                          <  2:3738268/101‑1 (MQ=255)
                       cTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCtt                                                                         <  1:2889719/101‑1 (MQ=255)
                       cTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCtt                                                                         <  2:3730570/101‑1 (MQ=255)
                       cTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCtt                                                                         <  1:1563112/101‑1 (MQ=255)
                        tCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTg                                                                        <  1:2032584/101‑1 (MQ=255)
                          gggTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGcc                                                                      >  1:2552856/1‑101 (MQ=255)
                            gTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGCCCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCt                                                                    <  1:1953228/101‑1 (MQ=255)
                            gTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCt                                                                    >  2:4219109/1‑101 (MQ=255)
                             tGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTg                                                                   >  1:1864717/1‑101 (MQ=255)
                             tGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTg                                                                   <  1:2739015/101‑1 (MQ=255)
                             tGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTg                                                                   <  2:2993787/101‑1 (MQ=255)
                                 gCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATgg                                                               >  1:752094/1‑101 (MQ=255)
                                 gCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATgg                                                               >  1:2786265/1‑101 (MQ=255)
                                 gCTGCATATGCTGGCCTCGGGCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATgg                                                               <  2:350791/101‑1 (MQ=255)
                                  cTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACACTTGCCCTGATggg                                                              >  1:658804/1‑101 (MQ=255)
                                   tGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGc                                                             >  1:221517/1‑101 (MQ=255)
                                   tGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGc                                                             >  1:2869258/1‑101 (MQ=255)
                                    gCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGcc                                                            >  2:1158326/1‑101 (MQ=255)
                                    gCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGcc                                                            <  2:1303458/101‑1 (MQ=255)
                                    gCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGcc                                                            <  2:4381076/101‑1 (MQ=255)
                                      atatGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGc                                                          >  2:1555051/1‑101 (MQ=255)
                                          gCTGGCCTCGGTCGTGGTGTTCGGCCCGGGCTTGCTGACCCCCGCGCTGCTCGCCGCCTGCGCCGAGTGTGCCATCACCCTGGCCCTGACGCGCCGCACTg                                                      >  1:1480643/1‑101 (MQ=255)
                                           cTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATgg                                                     <  1:2594777/101‑1 (MQ=255)
                                           cTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATgg                                                     >  1:3131941/1‑101 (MQ=255)
                                            tGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGTATCACCCTTGCCCTGATGGGCCGCAATGGc                                                    <  1:3967244/101‑1 (MQ=255)
                                            tGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGc                                                    >  2:3914283/1‑101 (MQ=255)
                                            tGGCCTCGGTCGTGGTGTTCGGCCAGGTCTTGCTGACCCCCGAGCTGATCGAAGCCTGCGACGAGAGCGGAATAACCCTTGCCCTGATGGGCCGCAATGGc                                                    >  1:4151737/1‑101 (MQ=255)
                                             ggCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGcc                                                   >  1:350692/1‑101 (MQ=255)
                                             ggCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGcc                                                   >  1:4170998/1‑101 (MQ=255)
                                             ggCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGAGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGcc                                                   >  1:2719564/1‑101 (MQ=255)
                                               ccTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGTATCACCCTTGCCCCGATGGGCCGCAATGGCCgg                                                 >  1:3786974/1‑101 (MQ=255)
                                                cTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGt                                                >  1:3566856/1‑101 (MQ=255)
                                                   ggTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTc                                             <  1:2685296/101‑1 (MQ=255)
                                                   ggTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTc                                             <  2:3163331/101‑1 (MQ=255)
                                                    gTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCa                                            <  2:458248/101‑1 (MQ=255)
                                                      cGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCCgg                                          >  2:588663/1‑101 (MQ=255)
                                                      cGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAgg                                          >  2:1820903/1‑101 (MQ=255)
                                                      cGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAgg                                          >  2:681931/1‑101 (MQ=255)
                                                      cGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAgg                                          >  1:2510345/1‑101 (MQ=255)
                                                       gtggtgTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGc                                         >  2:2059089/1‑101 (MQ=255)
                                                         ggtgTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGccc                                       >  1:1080695/1‑101 (MQ=255)
                                                         ggtgTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGccc                                       <  1:3137440/101‑1 (MQ=255)
                                                          gtgtTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCcg                                      >  1:2118568/1‑101 (MQ=255)
                                                          gtgtTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCcg                                      <  1:3010881/101‑1 (MQ=255)
                                                            gtTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCTCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCcgcg                                    <  1:3809606/101‑1 (MQ=255)
                                                             ttCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGt                                   <  1:4066020/101‑1 (MQ=255)
                                                              tCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTc                                  <  1:2160745/101‑1 (MQ=255)
                                                               cGGCCCGGTCTTGCTGCCCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCg                                 <  1:4257558/101‑1 (MQ=255)
                                                               cGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCg                                 <  2:2704524/101‑1 (MQ=255)
                                                               cGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCg                                 >  1:811388/1‑101 (MQ=255)
                                                                  cccGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAg                              <  2:3065617/101‑1 (MQ=255)
                                                                   ccGGTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAgg                             >  2:1998240/1‑101 (MQ=255)
                                                                    cGGTCTTGCTGACCCCCGCGCTGATCGCCACCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGc                            >  2:3312103/1‑101 (MQ=255)
                                                                     ggTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGcc                           >  2:1505216/1‑101 (MQ=255)
                                                                     ggTCTGCCTGCCCCCCGCGCGGACCGCCGCCGGCGCCGAGCGCGGCATCCCCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGcc                           <  2:2625032/101‑1 (MQ=255)
                                                                      gTCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGccc                          >  1:1892832/1‑101 (MQ=255)
                                                                       tCTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGTCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCg                         >  1:1300133/1‑101 (MQ=255)
                                                                        cTTGCTGCCCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCgg                        <  1:887345/101‑1 (MQ=255)
                                                                        cTTGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCgg                        >  1:4020271/1‑101 (MQ=255)
                                                                         ttGCTGCCCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGt                       <  1:4334918/101‑1 (MQ=255)
                                                                         ttGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGt                       >  2:2210750/1‑101 (MQ=255)
                                                                          tGCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGtc                      <  1:1897022/101‑1 (MQ=255)
                                                                           gCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGtct                     >  2:2371490/1‑101 (MQ=255)
                                                                           gCTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGtct                     >  2:1352211/1‑101 (MQ=255)
                                                                            cTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGtctc                    <  2:2916020/101‑1 (MQ=255)
                                                                            cTGACCCCCGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGtctc                    <  1:2392008/101‑1 (MQ=255)
                                                                              gccccccGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCgg                  <  1:1426272/99‑1 (MQ=255)
                                                                              gccccccGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCgg                  <  2:4275941/99‑1 (MQ=255)
                                                                                cccccGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGc                >  1:3760426/1‑101 (MQ=255)
                                                                                 ctccGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCa               >  2:4317054/3‑101 (MQ=255)
                                                                                 ccccGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCa               >  2:3369469/1‑101 (MQ=255)
                                                                                 ccccGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCa               <  1:2059226/101‑1 (MQ=255)
                                                                                  cccGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCaa              >  1:4137913/1‑101 (MQ=255)
                                                                                  cccGCGCTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCaa              >  1:333424/1‑101 (MQ=255)
                                                                                      cgcTGATCGCCGCCTGCGGCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGACCGGGCTCGGGGAAtgtg          >  2:1446742/1‑101 (MQ=255)
                                                                                      cgcTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCAAtgtg          <  2:250382/101‑1 (MQ=255)
                                                                                       gcTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCAATGTGc         >  2:629044/1‑101 (MQ=255)
                                                                                        cTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCAATGTGCt        >  1:3923391/1‑101 (MQ=255)
                                                                                        cTGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCAATGTGCt        >  1:3666295/1‑101 (MQ=255)
                                                                                         tGATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCAATGTGCTg       >  2:107057/1‑101 (MQ=255)
                                                                                          gATCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCAATGTGCTGt      >  2:1699796/1‑101 (MQ=255)
                                                                                           aTCGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCAATGTGCTGtt     <  1:2380377/101‑1 (MQ=255)
                                                                                             cGCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCAATGTGCTGTTgc   <  1:3451268/101‑1 (MQ=255)
                                                                                              gCCGCCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCAATGTGCTGTTgcg  <  2:4067312/101‑1 (MQ=255)
                                                                                                  |                                                                                                
CGAGATCGAGGGTGCCGACAAGACTCGGGTGCCGCTGCATATGCTGGCCTCGGTCGTGGTGTTCGGCCCGGTCTTGCTGACCCCCGCGCTGATCGCCGTCTGCGCCGAGCGCGGCATCACCCTTGCCCTGATGGGCCGCAATGGCCGGTTTCAGGCCCGCGTCGAAGGCCCGGTCTCGGGCAATGTGCTGTTGCG  >  NC_014034/1393939‑1394133

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG