Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,534 C→T 10.9% intergenic (+346/‑585) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5340C→T10.9% 72.3 ‑1.0 0.0318intergenic (+346/‑585)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (20/13):  ref base (132/125):  total (161/157)
Fisher's exact test strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 372 total aligned reads displayed.

GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGT  >  NC_014034/1395434‑1395634
                                                                                                    |                                                                                                        
   gCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                                                                                      <  1:2194230/101‑4 (MQ=2)
   gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGTTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                                                                                       >  1:3836163/1‑101 (MQ=255)
    cTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCgg                                                                                                      <  2:1680799/101‑1 (MQ=255)
    cTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCgg                                                                                                      <  1:2754068/101‑1 (MQ=255)
     tCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaa                                                                                                    >  2:826314/1‑96 (MQ=2)
     tCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaa                                                                                                    <  2:1220077/101‑6 (MQ=2)
     tCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaa                                                                                                    <  1:969584/101‑6 (MQ=2)
     tCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaa                                                                                                    >  1:672885/1‑96 (MQ=2)
       nccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGNTCGAAACNnnncnnnnn                                                                                                   >  2:1870241/2‑96 (MQ=255)
       cccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatg                                                                                                  >  2:3470297/1‑94 (MQ=2)
       cccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatg                                                                                                  >  1:4089173/1‑94 (MQ=2)
       cccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGc                                                                                                   >  2:3164606/1‑101 (MQ=255)
       cccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGc                                                                                                   <  1:2444051/101‑1 (MQ=255)
        ccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatgg                                                                                                 <  1:1764724/101‑9 (MQ=2)
        ccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGcc                                                                                                  <  2:1548118/101‑1 (MQ=255)
         cccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCg                                                                                                 <  2:2989944/101‑1 (MQ=255)
         cccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCg                                                                                                 <  1:297628/101‑1 (MQ=255)
             cgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGc                                                                                             >  2:2266321/1‑101 (MQ=255)
                gggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCg                                                                                          <  1:4179142/101‑1 (MQ=255)
                 ggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.t                                                                                        >  2:1940486/1‑101 (MQ=255)
                    gtggcgcGAAACGTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCACTCCCCCCGAGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCa                                                                                    <  2:4038273/95‑1 (MQ=38)
                      ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGc                                                                                  >  1:3769254/1‑101 (MQ=255)
                      ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGc                                                                                  >  2:4338005/1‑101 (MQ=255)
                        aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCg                                                                                >  1:162375/1‑101 (MQ=255)
                        aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACATCTCGGTGCCGTGGCTCG.T.CCAGCCg                                                                                >  2:2519687/1‑101 (MQ=255)
                          cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.c                                                                             >  2:3581973/1‑101 (MQ=255)
                            aaaCATCGCCGCTTTGGCGGTGGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCt                                                                           >  1:2546586/1‑101 (MQ=255)
                            aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCt                                                                           >  1:158756/1‑101 (MQ=255)
                                 tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCtcg                                                                     <  2:1184871/101‑1 (MQ=255)
                                  cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCtcgt                                                                    >  2:1029964/1‑101 (MQ=255)
                                        tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTcc                                                              >  1:2598093/1‑101 (MQ=255)
                                            gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTcccccc                                                          <  2:1982857/101‑1 (MQ=255)
                                            gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCGCGTCGCTcccccc                                                          >  2:1415436/1‑101 (MQ=255)
                                             cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTccccccc                                                         >  1:308252/1‑100 (MQ=255)
                                                  gACAGGCTGGGAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCCCgggg                                                    >  1:3044498/1‑101 (MQ=255)
                                                   aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCggggg                                                   >  1:194547/1‑101 (MQ=255)
                                                        cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTgg                                              <  2:3667401/101‑1 (MQ=255)
                                                        agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.TGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                              <  2:2096070/91‑1 (MQ=2)
                                                         gcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                             <  1:3181707/92‑1 (MQ=0)
                                                         aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGa                                             <  1:2635088/92‑1 (MQ=0)
                                                          ggTAACGTTTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  1:3452692/101‑1 (MQ=255)
                                                           ggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGGGGATc                                           >  1:3221567/8‑101 (MQ=0)
                                                           gccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:2849624/94‑1 (MQ=2)
                                                            tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          >  1:2408261/7‑101 (MQ=2)
                                                            gctgcgggcGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  1:3283593/93‑1 (MQ=2)
                                                             cgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGGGGATCGa                                         >  2:3075314/6‑101 (MQ=2)
                                                             aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGa                                         >  1:428770/1‑101 (MQ=255)
                                                              ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        >  1:2108022/5‑101 (MQ=2)
                                                              ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        >  1:3928751/5‑101 (MQ=2)
                                                              ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                        >  2:4089049/5‑101 (MQ=2)
                                                              ccgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.A.TCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         >  1:1698980/5‑101 (MQ=2)
                                                              aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  1:51831/101‑1 (MQ=255)
                                                               cacgtCGCTCCCCCCGTGGGGGCTTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  1:2814487/98‑1 (MQ=2)
                                                               cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  2:1786142/4‑101 (MQ=2)
                                                               cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.C.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  2:3851638/101‑1 (MQ=255)
                                                                ctgccccTCCCCCCGCGGGGGCGTGGATCCAAACACCTCGGTGCCGCCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                      <  2:4212068/95‑1 (MQ=37)
                                                                 ggtCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.CCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                     <  2:2891007/100‑2 (MQ=2)
                                                                 cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaat                                     >  2:70497/2‑99 (MQ=2)
                                                                  gtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccc                                    <  1:879407/101‑2 (MQ=2)
                                                                  ggcGCGCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccc                                    <  2:2685051/99‑2 (MQ=2)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                   <  2:2147390/101‑3 (MQ=2)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCCGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaaat                                   >  2:883853/1‑97 (MQ=2)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                   <  2:2611957/101‑4 (MQ=2)
                                                                   tCGCCCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                   <  1:3603015/101‑4 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                  >  1:445343/1‑101 (MQ=255)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                  >  1:4141306/1‑101 (MQ=255)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGAGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCTGCGGGGGCGTGGATCGAAactgcg                                  >  2:2648061/1‑97 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                  >  2:3720117/1‑98 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                  <  2:3549457/101‑5 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                  <  2:3336388/101‑5 (MQ=2)
                                                                     gccccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                 <  1:3151513/98‑5 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgt                                 >  1:2376134/1‑96 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgc                                 <  1:4034304/101‑6 (MQ=2)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                >  2:2359555/1‑101 (MQ=255)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                               >  1:3488125/1‑94 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                               <  2:386437/101‑8 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                               >  2:415182/1‑94 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                               <  2:1273044/101‑8 (MQ=2)
                                                                        ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACCACCTTGc                              >  2:2760103/1‑101 (MQ=255)
                                                                        ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCACCGCGGGGGCGTGGATCGAAACCACCTTGc                              <  1:1030052/101‑1 (MQ=255)
                                                                        ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacaccaatgc                              <  2:1615327/101‑9 (MQ=2)
                                                                        ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                              <  1:143049/101‑9 (MQ=2)
                                                                          ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGGGGATCGAAactatactggct                            >  1:1853914/1‑91 (MQ=2)
                                                                            ccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                          >  1:1543978/1‑101 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTgg                       >  2:2566796/1‑100 (MQ=255)
                                                                                 ggggCGTGGATCGAACCATCTTGGCGCCGTTGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCGTGCGGATTGTCa                     <  2:2432641/101‑1 (MQ=37)
                                                                                  gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                    <  1:2491531/101‑1 (MQ=255)
                                                                                    gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                  <  1:1880216/101‑1 (MQ=255)
                                                                                     cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                 <  1:816256/101‑1 (MQ=255)
                                                                                      gTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                >  1:3570217/1‑101 (MQ=255)
                                                                                       tGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg               <  1:1068494/101‑1 (MQ=255)
                                                                                        ggATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgc              <  1:1484285/101‑1 (MQ=255)
                                                                                          aTCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgg            >  1:4342196/1‑101 (MQ=255)
                                                                                            cGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCCCTTTCGCgggg          >  2:1096357/1‑101 (MQ=255)
                                                                                              aaaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg        <  2:2037602/101‑1 (MQ=255)
                                                                                              aaaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg        <  2:514822/101‑1 (MQ=255)
                                                                                               aaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa       <  1:2717524/101‑1 (MQ=255)
                                                                                               aaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa       >  1:1208190/1‑101 (MQ=255)
                                                                                                aCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGaa      <  1:1369093/101‑1 (MQ=255)
                                                                                                  aTCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc    >  2:1325554/1‑101 (MQ=255)
                                                                                                    |                                                                                                        
GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGT  >  NC_014034/1395434‑1395634

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG