Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,542 C→G 10.5% intergenic (+354/‑577) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5420C→G10.5% 71.1 ‑1.3 ‑0.3319intergenic (+354/‑577)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (20/13):  ref base (138/144):  total (160/159)
Fisher's exact test strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.14e-01

Only 100 of 347 total aligned reads displayed.

CCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCC  >  NC_014034/1395442‑1395642
                                                                                                    |                                                                                                       
ccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGcc                                                                                                         >  2:3440160/1‑101 (MQ=255)
 cccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCg                                                                                                        <  2:2989944/101‑1 (MQ=255)
  ccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGt                                                                                                       <  2:4028463/101‑1 (MQ=255)
     cgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCAGGGGCGTGGATCGAAACATCTCGGTGCCGTCGc                                                                                                    >  1:3788457/1‑101 (MQ=255)
        gggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCg                                                                                                 <  1:4179142/101‑1 (MQ=255)
          gCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.c                                                                                             >  2:3692906/1‑101 (MQ=255)
           cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.cc                                                                                            <  1:3579236/101‑1 (MQ=255)
            gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCa                                                                                           <  1:2572313/101‑1 (MQ=255)
            gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCa                                                                                           <  2:4178411/101‑1 (MQ=255)
             tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCac                                                                                          >  1:2123838/1‑100 (MQ=255)
              ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGc                                                                                         >  2:4338005/1‑101 (MQ=255)
              ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGc                                                                                         <  1:2397317/101‑1 (MQ=255)
                aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCg                                                                                       >  2:2323335/1‑101 (MQ=255)
                  cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.c                                                                                    >  1:3667654/1‑101 (MQ=255)
                      aCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGc                                                                                >  1:3165648/1‑101 (MQ=255)
                        aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtc                                                                              <  1:114314/101‑1 (MQ=255)
                          cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtcgt                                                                            >  2:1029964/1‑101 (MQ=255)
                                 ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTccc                                                                     >  2:4149107/1‑101 (MQ=255)
                                    gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTcccccc                                                                  <  2:1982857/101‑1 (MQ=255)
                                    gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCGCGTCGCTcccccc                                                                  >  2:1415436/1‑101 (MQ=255)
                                     cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCcg                                                                 >  1:1581279/1‑101 (MQ=255)
                                       gTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCcgcg                                                               >  1:2037739/1‑101 (MQ=255)
                                        tCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCCCgg                                                              >  2:1665840/1‑101 (MQ=255)
                                          gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCgggg                                                            >  2:2491368/1‑101 (MQ=255)
                                           aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCggggg                                                           <  1:2266466/101‑1 (MQ=255)
                                            cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGc                                                          <  1:1921584/101‑1 (MQ=255)
                                               gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTg                                                       >  2:2656371/1‑101 (MQ=255)
                                               gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTg                                                       <  1:2781424/101‑1 (MQ=255)
                                               gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCGGGCGG.CCTGCTCGGCGCTCCCCCCGCTGGGGCGTg                                                       >  2:1762548/1‑101 (MQ=38)
                                                cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTgg                                                      >  2:3129883/1‑101 (MQ=255)
                                                agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                      <  2:2253123/91‑1 (MQ=0)
                                                agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.TGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                      <  2:2096070/91‑1 (MQ=2)
                                                aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTgg                                                      >  1:486957/11‑101 (MQ=2)
                                                  ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                    <  1:1704250/93‑1 (MQ=2)
                                                   gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                   <  1:2953280/101‑1 (MQ=255)
                                                   ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                   >  2:4065638/8‑101 (MQ=2)
                                                      ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                >  1:3928751/5‑101 (MQ=2)
                                                      gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                >  2:3574842/5‑101 (MQ=2)
                                                      aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                <  1:51831/101‑1 (MQ=255)
                                                       gcggtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                               <  1:1618627/98‑1 (MQ=2)
                                                       ccggtCGCGCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCGGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                               <  2:3042668/98‑1 (MQ=2)
                                                       cacgtCGCTCCCCCCGTGGGGGCTTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                               <  1:2814487/98‑1 (MQ=2)
                                                         ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                             <  2:2144519/100‑1 (MQ=2)
                                                         ggtCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.CCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                             <  2:2891007/100‑2 (MQ=2)
                                                          gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCCCGGGGGCGTGGATCGCAactg                                            >  2:1610703/1‑99 (MQ=2)
                                                          gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacta                                            <  2:3646860/101‑3 (MQ=2)
                                                          gtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccc                                            <  1:879407/101‑2 (MQ=2)
                                                          ggcGCGCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccc                                            <  2:2685051/99‑2 (MQ=2)
                                                           tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                           <  2:2611957/101‑4 (MQ=2)
                                                           gcGCCCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                           <  2:1132644/100‑4 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                          >  1:4141306/1‑101 (MQ=255)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                          <  2:3336388/101‑5 (MQ=2)
                                                            cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                          <  2:3549457/101‑5 (MQ=2)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgt                                         >  1:4033531/1‑96 (MQ=2)
                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                         <  2:1502136/101‑5 (MQ=2)
                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                        <  1:2611548/101‑1 (MQ=255)
                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAACCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcg                                        <  2:825507/101‑6 (MQ=0)
                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                        <  1:3095245/101‑7 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                       <  2:1206696/101‑1 (MQ=255)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                       >  2:617795/1‑94 (MQ=2)
                                                               tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                       <  2:1273044/101‑8 (MQ=2)
                                                               cccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                       <  1:3422241/100‑1 (MQ=255)
                                                                ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACCACCTTGc                                      >  2:2760103/1‑101 (MQ=255)
                                                                ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCACCGCGGGGGCGTGGATCGAAACCACCTTGc                                      <  1:1030052/101‑1 (MQ=255)
                                                                ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcg                                      >  1:2471703/1‑93 (MQ=2)
                                                                ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                      >  1:3492231/1‑94 (MQ=2)
                                                                ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                      <  1:143049/101‑9 (MQ=2)
                                                                 cccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCg                                     >  1:4253530/1‑101 (MQ=255)
                                                                 cccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgc                                     >  2:840736/1‑92 (MQ=2)
                                                                  ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggct                                    <  1:2672591/101‑11 (MQ=2)
                                                                  ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGGGGATCGAAactatactggct                                    >  1:1853914/1‑91 (MQ=2)
                                                                    ccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAGCACCTTGCGGAt                                  <  1:2418207/101‑1 (MQ=255)
                                                                     cgcgGGGGCGTGGATCGAAACCTCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                 <  1:693056/101‑1 (MQ=255)
                                                                      gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                                <  2:1847675/101‑1 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTgg                               >  2:2566796/1‑100 (MQ=255)
                                                                       cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                               <  1:3184118/101‑1 (MQ=255)
                                                                        gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                              >  1:997113/1‑101 (MQ=255)
                                                                         ggggCGTGGATCGAACCATCTTGGCGCCGTTGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCGTGCGGATTGTCa                             <  2:2432641/101‑1 (MQ=37)
                                                                          gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                            >  1:4078246/1‑101 (MQ=255)
                                                                           ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                           >  1:1667766/1‑101 (MQ=255)
                                                                            gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                          <  1:1880216/101‑1 (MQ=255)
                                                                            gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                          <  1:3272330/101‑1 (MQ=255)
                                                                             cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                         <  1:816256/101‑1 (MQ=255)
                                                                               tGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                       <  1:1068494/101‑1 (MQ=255)
                                                                               tGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                       <  2:385895/101‑1 (MQ=255)
                                                                                ggATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgc                      >  1:3064455/1‑101 (MQ=255)
                                                                                 gATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                     <  1:3473716/101‑1 (MQ=255)
                                                                                  aTCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgg                    >  1:4342196/1‑101 (MQ=255)
                                                                                   tCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCCCTTTCGCggg                   >  1:1531161/1‑101 (MQ=255)
                                                                                   tCGAAACATCTCGGTGCCGCCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCCTTGCGGATTGTCACTTTCGCggg                   <  1:2519850/101‑1 (MQ=255)
                                                                                      aaaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                <  2:2037602/101‑1 (MQ=255)
                                                                                       aaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa               <  1:2717524/101‑1 (MQ=255)
                                                                                       aaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa               <  2:3835187/101‑1 (MQ=255)
                                                                                       aaCACCTCGGGGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa               <  2:1955725/101‑1 (MQ=255)
                                                                                        aCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGaa              <  1:1369093/101‑1 (MQ=255)
                                                                                          aTCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc            >  2:1325554/1‑101 (MQ=255)
                                                                                            ctcGGGGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACCACCTGGCGGATTGTCACTTTCGCGGGGCGAAGCGt          >  1:298386/1‑101 (MQ=255)
                                                                                              cGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg        <  2:428730/101‑1 (MQ=255)
                                                                                                 tGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTc     >  2:500610/1‑101 (MQ=255)
                                                                                                  gCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCCcc    >  1:4317755/1‑101 (MQ=255)
                                                                                                    |                                                                                                       
CCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCC  >  NC_014034/1395442‑1395642

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG