Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,547 C→A 8.9% intergenic (+359/‑572) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5470C→A 8.9% 68.8 ‑3.0 ‑0.5313intergenic (+359/‑572)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (9/19):  ref base (149/136):  total (158/155)
Fisher's exact test strand distribution p-value = 4.84e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.22e-01

Only 100 of 343 total aligned reads displayed.

CGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCG  >  NC_014034/1395447‑1395647
                                                                                                    |                                                                                                        
cgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCAGGGGCGTGGATCGAAACATCTCGGTGCCGTCGc                                                                                                          >  1:3788457/1‑101 (MQ=255)
    ggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.t                                                                                                     >  2:1940486/1‑101 (MQ=255)
      cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTACCGTGTCGCTCCCCCCGCGGGGGCCTGGGTCGAAACATTTCGGTACCGCCACTCG.C.cc                                                                                                  >  1:2432798/1‑101 (MQ=37)
      cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.cc                                                                                                  <  1:3579236/101‑1 (MQ=255)
        tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCac                                                                                                >  1:2123838/1‑100 (MQ=255)
           aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCg                                                                                             >  2:2323335/1‑101 (MQ=255)
             cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.c                                                                                          >  1:3667654/1‑101 (MQ=255)
               aaaCATCGCCGCTTTGGCGGTGGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCt                                                                                        >  1:2546586/1‑101 (MQ=255)
                   aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCtc                                                                                   <  1:2519588/101‑1 (MQ=255)
                   aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCtc                                                                                   <  1:114314/101‑1 (MQ=255)
                     cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCtcgt                                                                                 >  2:1029964/1‑101 (MQ=255)
                     cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCC.T.CCCGCCGG.CCT.GCtcgt                                                                                 >  2:4301230/1‑101 (MQ=255)
                      gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCtcgtc                                                                                <  2:3885209/101‑1 (MQ=255)
                      gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCtcgtc                                                                                >  2:3678087/1‑101 (MQ=255)
                      gccgcTTTGGCGGTCGACAGGCTGGTAACGTGACGCTCCCCCCGCGGGGGAGTGGATCGAAACATCTCGGTGCCGCCGCTCG.T.CCAGCCGG.CCG.CCtcgtc                                                                                >  1:2790155/1‑101 (MQ=38)
                           tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTcc                                                                           >  1:2598093/1‑101 (MQ=255)
                           tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCCCGGGGGCGTGGGTCGGAAAACCTCGGTGCCGTCGCTCG.T.CCAGACGG.CCC.GCTCGTCGCTcc                                                                           >  1:3182344/1‑101 (MQ=255)
                               gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTcccccc                                                                       <  2:1982857/101‑1 (MQ=255)
                                cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTccccccc                                                                      >  1:308252/1‑100 (MQ=255)
                                   tCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCCCgg                                                                   >  2:1665840/1‑101 (MQ=255)
                                     gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCgggg                                                                 >  2:2491368/1‑101 (MQ=255)
                                     gACAGGCTGGGAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCCCgggg                                                                 >  1:3044498/1‑101 (MQ=255)
                                      aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCggggg                                                                <  1:2266466/101‑1 (MQ=255)
                                      aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCggggg                                                                >  1:1770755/1‑101 (MQ=255)
                                      aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCggggg                                                                >  1:194547/1‑101 (MQ=255)
                                          gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTg                                                            >  2:1127315/1‑101 (MQ=255)
                                          gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCGGGCGG.CCT.GCTCGGCGCTCCCCCCGCTGGGGCGTg                                                            >  2:1762548/1‑101 (MQ=38)
                                           cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:4315416/101‑1 (MQ=255)
                                           cTGGAAGCGTGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCTCGGTGCGGTCACTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:167216/101‑1 (MQ=38)
                                           agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  2:2253123/91‑1 (MQ=0)
                                           agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.TGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  2:2096070/91‑1 (MQ=2)
                                            tGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGa                                                          >  1:186720/1‑101 (MQ=255)
                                            gcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  2:3594909/92‑1 (MQ=0)
                                            ccctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  1:1581285/92‑1 (MQ=2)
                                            aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  1:2635088/92‑1 (MQ=0)
                                              ggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGGGGATc                                                        >  1:3221567/8‑101 (MQ=0)
                                              gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:726867/101‑1 (MQ=255)
                                              gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        >  2:1484180/1‑101 (MQ=255)
                                              ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        >  2:4065638/8‑101 (MQ=2)
                                              ataccgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.A.TCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         >  2:2542467/8‑101 (MQ=0)
                                               tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       >  1:1884232/7‑101 (MQ=2)
                                                cgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGGGGATCGa                                                      >  2:3075314/6‑101 (MQ=2)
                                                 ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     >  1:3928751/5‑101 (MQ=2)
                                                 ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     <  1:3439544/97‑1 (MQ=2)
                                                 aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     <  1:51831/101‑1 (MQ=255)
                                                  gcggtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    <  1:1618627/98‑1 (MQ=2)
                                                  cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    >  2:4106974/1‑101 (MQ=255)
                                                  cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    >  2:3851969/1‑101 (MQ=255)
                                                   ctgccccTCCCCCCGCGGGGGCGTGGATCCAAACACCTCGGTGCCGCCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                   <  2:4212068/95‑1 (MQ=37)
                                                   cggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                   <  1:2847619/99‑1 (MQ=2)
                                                    ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                  <  2:2144519/100‑1 (MQ=2)
                                                    ggtCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.CCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                  <  2:2891007/100‑2 (MQ=2)
                                                     gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCCCGGGGGCGTGGATCGCAactg                                                 >  2:1610703/1‑99 (MQ=2)
                                                     gtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccc                                                 <  1:879407/101‑2 (MQ=2)
                                                     ggcGCGCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccc                                                 <  2:2685051/99‑2 (MQ=2)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                                <  2:2611957/101‑4 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                               >  1:627229/1‑101 (MQ=255)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                               >  1:445343/1‑101 (MQ=255)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                               >  2:1383603/1‑101 (MQ=255)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                               >  1:4141306/1‑101 (MQ=255)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGAGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCTGCGGGGGCGTGGATCGAAactgcg                                               >  2:2648061/1‑97 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                               >  2:3720117/1‑98 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                               <  2:3336388/101‑5 (MQ=2)
                                                        gccccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                              <  1:3151513/98‑5 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgt                                              >  1:4033531/1‑96 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                              >  1:2968658/1‑97 (MQ=2)
                                                         cgccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                             <  2:1316548/99‑7 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtg                                             <  1:3486444/101‑7 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcg                                             >  2:3171748/1‑96 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                            <  2:1206696/101‑1 (MQ=255)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                            >  2:754725/1‑101 (MQ=255)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                            >  2:353153/1‑94 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACCACCTTGc                                           >  2:2760103/1‑101 (MQ=255)
                                                           ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCACCGCGGGGGCGTGGATCGAAACCACCTTGc                                           <  1:1030052/101‑1 (MQ=255)
                                                           ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                           <  2:2386913/101‑8 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                           <  1:1330008/101‑8 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                           <  1:954931/101‑9 (MQ=2)
                                                            cccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCg                                          >  1:4253530/1‑101 (MQ=255)
                                                            cccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgc                                          >  2:840736/1‑92 (MQ=2)
                                                            cccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcg                                          >  2:4161767/1‑93 (MQ=0)
                                                             ccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCTCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCgg                                         >  2:482597/1‑101 (MQ=255)
                                                              cccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGa                                        >  1:2745835/1‑101 (MQ=255)
                                                               ccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                                       >  1:1543978/1‑101 (MQ=255)
                                                                 gcgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTg                                     <  2:1847675/101‑1 (MQ=255)
                                                                  cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                                    <  1:2400753/101‑1 (MQ=255)
                                                                   gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCCAAAACACCTTGCGGATTGTc                                   >  2:1265556/1‑101 (MQ=255)
                                                                     gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                                 >  1:4078246/1‑101 (MQ=255)
                                                                         gTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                             <  2:3018930/101‑1 (MQ=255)
                                                                          tGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                            <  1:1068494/101‑1 (MQ=255)
                                                                           ggATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgc                           <  1:1484285/101‑1 (MQ=255)
                                                                            gATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                          >  1:4172500/1‑101 (MQ=255)
                                                                             aTCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgg                         >  1:4342196/1‑101 (MQ=255)
                                                                                gAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                      >  2:203211/1‑101 (MQ=255)
                                                                                 aaaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                     <  2:514822/101‑1 (MQ=255)
                                                                                      tctcGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg                >  1:2702784/1‑101 (MQ=255)
                                                                                            tGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTc          >  1:3340895/1‑101 (MQ=255)
                                                                                              ccGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccc        <  2:3569969/101‑1 (MQ=255)
                                                                                                gTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACCACCTTGCGGGTTGTCACTTTCGCGGGGGGGAGGGTCGCTccccc      >  1:1389226/1‑101 (MQ=38)
                                                                                                 tCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc     <  1:2051075/101‑1 (MQ=255)
                                                                                                    cTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgcg  >  1:3519958/1‑101 (MQ=255)
                                                                                                    |                                                                                                        
CGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCG  >  NC_014034/1395447‑1395647

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG