Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,554 A→G 17.3% intergenic (+366/‑565) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5540A→G17.3% 104.1 ‑1.7 ‑0.6314intergenic (+366/‑565)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (17/26):  ref base (120/115):  total (159/155)
Fisher's exact test strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.65e-01

Only 100 of 342 total aligned reads displayed.

GTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1395454‑1395654
                                                                                                      |                                                                                                     
gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCa                                                                                                       <  2:4178411/101‑1 (MQ=255)
 tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAg                                                                                                      >  1:145093/1‑101 (MQ=255)
   gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGcc                                                                                                    >  2:2781234/1‑101 (MQ=255)
    aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCg                                                                                                   >  2:2323335/1‑101 (MQ=255)
      cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.c                                                                                                >  1:3667654/1‑101 (MQ=255)
       gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCCGCCGG.cc                                                                                               >  1:1955854/1‑101 (MQ=255)
        aaaCATCGCCGCTTTGGCGGTGGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCt                                                                                              >  1:2546586/1‑101 (MQ=255)
        aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCt                                                                                              >  2:1981166/1‑101 (MQ=255)
        aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCt                                                                                              >  2:3734775/1‑101 (MQ=255)
          aCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGc                                                                                            >  1:3951343/1‑101 (MQ=255)
            aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtc                                                                                          <  1:2519588/101‑1 (MQ=255)
             tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtcg                                                                                         >  1:160646/1‑101 (MQ=255)
             tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtcg                                                                                         <  2:1184871/101‑1 (MQ=255)
              cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCC.T.CCCGCCGG.CCTGCtcgt                                                                                        >  2:4301230/1‑101 (MQ=255)
               gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtcgtc                                                                                       >  2:3678087/1‑101 (MQ=255)
               gccgcTTTGGCGGTCGACAGGCTGGTAACGTGACGCTCCCCCCGCGGGGGAGTGGATCGAAACATCTCGGTGCCGCCGCTCG.T.CCAGCCGG.CCGCCtcgtc                                                                                       >  1:2790155/1‑101 (MQ=38)
                ccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtcgtcg                                                                                      <  2:2726385/101‑1 (MQ=255)
                 cgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCACGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGc                                                                                     >  2:2981382/1‑101 (MQ=255)
                   cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTc                                                                                   >  2:2578642/1‑101 (MQ=255)
                    tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTcc                                                                                  >  1:2598093/1‑101 (MQ=255)
                          ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCcgc                                                                            >  1:3022552/1‑101 (MQ=255)
                              gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCgggg                                                                        >  2:2491368/1‑101 (MQ=255)
                                cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGc                                                                      <  2:1855227/101‑1 (MQ=255)
                                   nnnnnnnaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGtn                                                                   <  1:1870365/94‑2 (MQ=255)
                                   gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTg                                                                   >  2:2656371/1‑101 (MQ=255)
                                    agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.TGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                                  <  2:2096070/91‑1 (MQ=2)
                                    aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTgg                                                                  >  1:1571544/11‑101 (MQ=2)
                                     gcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 <  1:3181707/92‑1 (MQ=0)
                                     aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 <  1:2635088/92‑1 (MQ=0)
                                      gggaaCGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                <  2:1303047/98‑1 (MQ=255)
                                      ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                <  1:1704250/93‑1 (MQ=2)
                                       gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  1:726867/101‑1 (MQ=255)
                                       gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               <  1:2953280/101‑1 (MQ=255)
                                       ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               >  2:4065638/8‑101 (MQ=2)
                                        gctgcgggcGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  1:3283593/93‑1 (MQ=2)
                                        ccgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              <  2:828281/95‑1 (MQ=2)
                                         cggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             >  2:2618932/6‑101 (MQ=2)
                                          gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGGGGATCgca                                                            >  1:847854/5‑99 (MQ=2)
                                           gtggtCGCCCCCCCCGCGGGGGCGCGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           <  1:3480137/98‑1 (MQ=2)
                                           cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           >  2:4106974/1‑101 (MQ=255)
                                            ctgccccTCCCCCCGCGGGGGCGTGGATCCAAACACCTCGGTGCCGCCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                          <  2:4212068/95‑1 (MQ=37)
                                            cggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                          <  1:2847619/99‑1 (MQ=2)
                                             tgtCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                         <  1:1130060/101‑1 (MQ=255)
                                             ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                         <  1:1300839/100‑2 (MQ=2)
                                             cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaat                                                         >  2:70497/2‑99 (MQ=2)
                                              gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactg                                                        <  2:1013000/101‑3 (MQ=2)
                                              gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactc                                                        >  2:1347244/1‑99 (MQ=2)
                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                                       >  2:3636307/1‑101 (MQ=255)
                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                                       <  2:1513480/101‑3 (MQ=2)
                                               ccGCCCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGCCCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                                       <  2:123644/100‑4 (MQ=2)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                                      >  1:627229/1‑101 (MQ=255)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                                      >  1:445343/1‑101 (MQ=255)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                                      <  1:1774709/101‑4 (MQ=2)
                                                 gccccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                                     <  1:3151513/98‑5 (MQ=2)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgt                                                     >  1:2376134/1‑96 (MQ=2)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgc                                                     <  1:4034304/101‑6 (MQ=2)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                                    >  1:1914928/1‑101 (MQ=255)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                                    <  2:162354/101‑1 (MQ=255)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                                    <  1:1370187/101‑7 (MQ=2)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                   >  1:245244/1‑101 (MQ=255)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                                   >  2:353153/1‑94 (MQ=2)
                                                   cccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                   <  1:3422241/100‑1 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                                  >  1:4202298/1‑101 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCACCGCGGGGGCGTGGATCGAAACCACCTTGc                                                  <  1:1030052/101‑1 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAactgcgtgcg                                                  >  1:3209031/1‑93 (MQ=2)
                                                    ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                                  >  1:3492231/1‑94 (MQ=2)
                                                    ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                                  <  1:954931/101‑9 (MQ=2)
                                                     cccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgc                                                 >  2:3099463/1‑92 (MQ=2)
                                                     cccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                                 >  2:2200738/1‑92 (MQ=2)
                                                      ccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCTCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCgg                                                >  2:482597/1‑101 (MQ=255)
                                                      ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggct                                                >  1:307041/1‑91 (MQ=2)
                                                       cccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGa                                               <  2:3022331/101‑1 (MQ=255)
                                                       cccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGa                                               >  1:2745835/1‑101 (MQ=255)
                                                        ccGCGGGGGCGTGGATCGAAACATCGCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                                              <  1:4123184/101‑1 (MQ=255)
                                                         cgcgGGGGGGTGGATCGAAACATCTCGGTGCCGTCGCCCC.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                             <  1:4301499/101‑1 (MQ=255)
                                                           cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                                           <  1:2400753/101‑1 (MQ=255)
                                                             ggggCGTGGATCGAACCATCTTGGCGCCGTTGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCGTGCGGATTGTCa                                         <  2:2432641/101‑1 (MQ=37)
                                                              gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAATGTCAc                                        >  1:514874/1‑101 (MQ=255)
                                                               ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                                       >  1:1667766/1‑101 (MQ=255)
                                                                  gTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                    >  1:3570217/1‑101 (MQ=255)
                                                                   tGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                                   <  1:1068494/101‑1 (MQ=255)
                                                                   tGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                                   <  2:385895/101‑1 (MQ=255)
                                                                     gATCGAAACAGCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                                 <  2:168255/101‑1 (MQ=255)
                                                                      aTCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgg                                <  1:4149368/101‑1 (MQ=255)
                                                                       tCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCCCTTTCGCggg                               >  1:1531161/1‑101 (MQ=255)
                                                                        cGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCCCTTTCGCgggg                              >  2:1096357/1‑101 (MQ=255)
                                                                          aaaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                            <  2:514822/101‑1 (MQ=255)
                                                                          aaaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                            <  2:2037602/101‑1 (MQ=255)
                                                                           aaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa                           <  1:2717524/101‑1 (MQ=255)
                                                                             cctctcGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                         <  2:3182117/99‑1 (MQ=255)
                                                                             cATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAGCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                         >  1:3789093/1‑101 (MQ=255)
                                                                                  cGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg                    <  2:1439037/101‑1 (MQ=255)
                                                                                    gTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCt                  >  1:4324375/1‑101 (MQ=255)
                                                                                    gTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCt                  >  2:3362133/1‑101 (MQ=255)
                                                                                       ccGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccc               <  2:3569969/101‑1 (MQ=255)
                                                                                        cGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccc              <  1:2089065/101‑1 (MQ=255)
                                                                                        cGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccc              >  2:3910437/1‑101 (MQ=255)
                                                                                              tCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgg        <  1:2208265/101‑1 (MQ=255)
                                                                                                     cAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCg   <  2:1770636/101‑1 (MQ=255)
                                                                                                      aGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGt  <  2:2123699/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
GTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1395454‑1395654

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG