Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,555 G→T 13.0% intergenic (+367/‑564) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5550G→T13.0% 97.1 ‑2.5 ‑0.0315intergenic (+367/‑564)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/24):  ref base (135/113):  total (159/156)
Fisher's exact test strand distribution p-value = 8.39e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.16e-01

Only 100 of 339 total aligned reads displayed.

TGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1395455‑1395655
                                                                                                      |                                                                                                     
 ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGc                                                                                                      >  1:4245291/1‑101 (MQ=255)
 ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGc                                                                                                      >  2:4338005/1‑101 (MQ=255)
  gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGcc                                                                                                     >  2:2781234/1‑101 (MQ=255)
   aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCg                                                                                                    >  2:2323335/1‑101 (MQ=255)
     cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.c                                                                                                 >  2:426963/1‑101 (MQ=255)
      gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCCGCCGG.cc                                                                                                >  1:1955854/1‑101 (MQ=255)
       aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCt                                                                                               >  2:1981166/1‑101 (MQ=255)
       aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCt                                                                                               >  2:3734775/1‑101 (MQ=255)
         aCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGc                                                                                             >  1:3951343/1‑101 (MQ=255)
           aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtc                                                                                           <  1:2519588/101‑1 (MQ=255)
           aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGGCGCTCG.T.CCAGCCGG.CCTGCtc                                                                                           >  2:3098145/1‑101 (MQ=255)
            tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtcg                                                                                          <  2:1184871/101‑1 (MQ=255)
            tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtcg                                                                                          >  1:160646/1‑101 (MQ=255)
             cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtcgt                                                                                         >  2:1029964/1‑101 (MQ=255)
               ccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtcgtcg                                                                                       <  2:2726385/101‑1 (MQ=255)
                   tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCCCGGGGGCGTGGGTCGGAAAACCTCGGTGCCGTCGCTCG.T.CCAGACGG.CCCGCTCGTCGCTcc                                                                                   >  1:3182344/1‑101 (MQ=255)
                      ggCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTccccc                                                                                >  2:3473476/1‑101 (MQ=255)
                        cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTccccccc                                                                              >  1:308252/1‑100 (MQ=255)
                        cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCcg                                                                              <  2:4191464/101‑1 (MQ=255)
                         ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCcgc                                                                             >  2:2921769/1‑101 (MQ=255)
                             gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCgggg                                                                         >  2:2491368/1‑101 (MQ=255)
                             gACAGGCTGGGAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCCCgggg                                                                         >  1:3044498/1‑101 (MQ=255)
                               cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGc                                                                       <  2:1855227/101‑1 (MQ=255)
                                 ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGt                                                                     <  1:1704132/101‑1 (MQ=255)
                                  gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTg                                                                    >  2:2656371/1‑101 (MQ=255)
                                  gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTg                                                                    <  1:2742248/101‑1 (MQ=255)
                                   cTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  2:3667401/101‑1 (MQ=255)
                                   agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.TGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                                   <  2:2096070/91‑1 (MQ=2)
                                   aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTgg                                                                   >  1:1571544/11‑101 (MQ=2)
                                    gcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                  <  2:3594909/92‑1 (MQ=0)
                                     gggaaCGTGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                 <  2:1303047/98‑1 (MQ=255)
                                      ggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGGGGATc                                                                >  1:3221567/8‑101 (MQ=0)
                                      gccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  1:2849624/94‑1 (MQ=2)
                                      gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                <  1:726867/101‑1 (MQ=255)
                                      ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                >  2:549093/8‑101 (MQ=2)
                                      ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                >  2:4065638/8‑101 (MQ=2)
                                       tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               >  1:2408261/7‑101 (MQ=2)
                                       ccgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                               <  2:828281/95‑1 (MQ=2)
                                        cggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                              >  2:2618932/6‑101 (MQ=2)
                                         ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             <  1:3439544/97‑1 (MQ=2)
                                         aCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                             <  1:51831/101‑1 (MQ=255)
                                          cacgtCGCTCCCCCCGTGGGGGCTTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            <  1:2814487/98‑1 (MQ=2)
                                          cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  2:4106974/1‑101 (MQ=255)
                                          cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                            >  2:3851969/1‑101 (MQ=255)
                                           cggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                           <  1:4062880/99‑1 (MQ=2)
                                            tgtCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                          <  1:4161945/101‑1 (MQ=255)
                                            tgtCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                          <  1:1130060/101‑1 (MQ=255)
                                            ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                          >  1:2571264/2‑101 (MQ=2)
                                            ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                          <  1:1300839/100‑2 (MQ=2)
                                            cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaat                                                          >  2:70497/2‑99 (MQ=2)
                                             gtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccc                                                         <  1:879407/101‑2 (MQ=2)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                                        <  2:1513480/101‑3 (MQ=2)
                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                                        <  2:486905/101‑4 (MQ=2)
                                              gcGCCCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                                        <  2:1362712/100‑4 (MQ=2)
                                               cGCTCCCCCCGCGGTGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcg                                                       <  1:3843414/101‑5 (MQ=2)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                                       >  1:627229/1‑101 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                                       >  1:445343/1‑101 (MQ=255)
                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                                       <  1:1774709/101‑4 (MQ=2)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgt                                                      >  1:2376134/1‑96 (MQ=2)
                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                                      >  1:2968658/1‑97 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                                     <  1:2611548/101‑1 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                                     <  2:162354/101‑1 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                                     >  1:1914928/1‑101 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtg                                                     <  1:3486444/101‑7 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcg                                                     >  2:3171748/1‑96 (MQ=2)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                                     <  1:1370187/101‑7 (MQ=2)
                                                  cccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                    <  1:4338274/100‑1 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCACCGCGGGGGCGTGGATCGAAACCACCTTGc                                                   <  1:1030052/101‑1 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                                   <  1:143049/101‑9 (MQ=2)
                                                   ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                                   <  1:954931/101‑9 (MQ=2)
                                                   ccccccGCGGGGGCGTCGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                                   <  2:1247086/101‑1 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                                  >  2:2200738/1‑92 (MQ=2)
                                                     ccccGCGGGGGCGTTGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgcg                                                 <  1:1869934/101‑11 (MQ=0)
                                                      cccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGa                                                <  2:3022331/101‑1 (MQ=255)
                                                       ccGCGGGGGCGTGGATCGAAACATCGCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                                               <  1:4123184/101‑1 (MQ=255)
                                                        cgcgGGGGGGTGGATCGAAACATCTCGGTGCCGTCGCCCC.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                              <  1:4301499/101‑1 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTgg                                            >  2:2566796/1‑100 (MQ=255)
                                                            ggggCGTGGATCGAACCATCTTGGCGCCGTTGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCGTGCGGATTGTCa                                          <  2:2432641/101‑1 (MQ=37)
                                                              ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                                        >  1:1667766/1‑101 (MQ=255)
                                                                  tGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                                    <  1:1068494/101‑1 (MQ=255)
                                                                    gATCGAAACAGCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                                  <  2:168255/101‑1 (MQ=255)
                                                                      tCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCCCTTTCGCggg                                >  1:1531161/1‑101 (MQ=255)
                                                                       cGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCCCTTTCGCgggg                               >  2:1096357/1‑101 (MQ=255)
                                                                       cGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgggg                               <  1:3164830/101‑1 (MQ=255)
                                                                         aaaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                             <  2:2037602/101‑1 (MQ=255)
                                                                           aCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGaa                           <  1:3582221/101‑1 (MQ=255)
                                                                             aTCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                         <  1:2219277/101‑1 (MQ=255)
                                                                             aTCTCGGTGCCGTCGCTCG.T.CCAGCCCG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                         <  1:3735028/101‑1 (MQ=255)
                                                                                tcGGTGCCGTCTCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc                      <  1:2656543/101‑1 (MQ=255)
                                                                                 cGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg                     <  2:1439037/101‑1 (MQ=255)
                                                                                   gTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCt                   >  2:3362133/1‑101 (MQ=255)
                                                                                    tGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTc                  >  1:3340895/1‑101 (MQ=255)
                                                                                      ccGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccc                <  2:3569969/101‑1 (MQ=255)
                                                                                       cGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccc               >  2:3910437/1‑101 (MQ=255)
                                                                                         tCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc             <  1:2051075/101‑1 (MQ=255)
                                                                                           gCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgc           <  1:2759657/101‑1 (MQ=255)
                                                                                            cTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgcg          >  1:3519958/1‑101 (MQ=255)
                                                                                                 t.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCggggg      >  1:2459711/1‑101 (MQ=255)
                                                                                                    cAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCg    <  2:1770636/101‑1 (MQ=255)
                                                                                                     aGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGt   <  2:2123699/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
TGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTG  >  NC_014034/1395455‑1395655

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG