Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,556 C→A 11.0% intergenic (+368/‑563) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5560C→A11.0% 75.5 ‑1.0 ‑0.0308intergenic (+368/‑563)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (20/14):  ref base (134/140):  total (154/154)
Fisher's exact test strand distribution p-value = 3.63e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 338 total aligned reads displayed.

GGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1395456‑1395656
                                                                                                      |                                                                                                      
ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGc                                                                                                        >  2:4338005/1‑101 (MQ=255)
ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGc                                                                                                        >  1:3769254/1‑101 (MQ=255)
  aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACATCTCGGTGCCGTGGCTCG.T.CCAGCCg                                                                                                      >  2:2519687/1‑101 (MQ=255)
    cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.c                                                                                                   >  2:426963/1‑101 (MQ=255)
        aCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.Gc                                                                                              >  1:3165648/1‑101 (MQ=255)
         cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCt                                                                                             <  1:2632478/101‑1 (MQ=255)
          aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCtc                                                                                            <  1:544992/101‑1 (MQ=255)
                 cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTc                                                                                     >  1:84126/1‑101 (MQ=255)
                      gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTcccccc                                                                                <  2:1982857/101‑1 (MQ=255)
                       cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCcg                                                                               >  1:1581279/1‑101 (MQ=255)
                       cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCcg                                                                               <  2:4191464/101‑1 (MQ=255)
                        ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCcgc                                                                              >  2:2642920/1‑101 (MQ=255)
                            gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCgggg                                                                          >  2:1000043/1‑101 (MQ=255)
                             aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCggggg                                                                         <  1:715024/101‑1 (MQ=255)
                              cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGc                                                                        <  2:1855227/101‑1 (MQ=255)
                                 nnnnnnnaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGtn                                                                     <  1:1870365/94‑2 (MQ=255)
                                 gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTg                                                                     >  2:1127315/1‑101 (MQ=255)
                                 gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTg                                                                     <  1:2781424/101‑1 (MQ=255)
                                  cTGGAAGCGTGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCTCGGTGCGGTCACTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTgg                                                                    <  1:167216/101‑1 (MQ=38)
                                  agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                                    >  1:3113360/11‑101 (MQ=0)
                                  agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                                    <  2:2253123/91‑1 (MQ=0)
                                  agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.TGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                                    <  2:2096070/91‑1 (MQ=2)
                                   aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                                   >  2:4069680/10‑101 (MQ=0)
                                    ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  1:1704250/93‑1 (MQ=2)
                                     gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 <  2:160625/101‑1 (MQ=255)
                                     ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 >  2:549093/8‑101 (MQ=2)
                                      tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                >  1:2408261/7‑101 (MQ=2)
                                      tAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  1:2395409/101‑1 (MQ=255)
                                      ccgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  2:828281/95‑1 (MQ=2)
                                       cggtggtCGCGCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                               <  1:2742676/96‑1 (MQ=2)
                                       aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGa                                                               >  1:428770/1‑101 (MQ=255)
                                        ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  1:3928751/5‑101 (MQ=2)
                                        gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  2:3574842/5‑101 (MQ=2)
                                         gcggtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             <  1:1618627/98‑1 (MQ=2)
                                         ccggtCGCGCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCGGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             <  2:3042668/98‑1 (MQ=2)
                                         cacgtCGCTCCCCCCGTGGGGGCTTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             <  1:2814487/98‑1 (MQ=2)
                                         cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             >  2:1786142/4‑101 (MQ=2)
                                           tgtCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                           <  1:1130060/101‑1 (MQ=255)
                                           ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                           >  1:2571264/2‑101 (MQ=2)
                                           ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                           <  1:1300839/100‑2 (MQ=2)
                                           cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.A.TCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                            <  1:2229905/100‑1 (MQ=2)
                                           cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaat                                                           >  2:70497/2‑99 (MQ=2)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                                         >  2:3636307/1‑101 (MQ=255)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                                         >  2:2982962/1‑101 (MQ=255)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                                         <  2:2147390/101‑3 (MQ=2)
                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                                         <  2:486905/101‑4 (MQ=2)
                                             gcGCCCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                                         <  2:1362712/100‑4 (MQ=2)
                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                                        >  1:4141306/1‑101 (MQ=255)
                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgt                                                       >  1:4033531/1‑96 (MQ=2)
                                                cgccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                                      <  2:1316548/99‑7 (MQ=2)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                                      >  2:2359555/1‑101 (MQ=255)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                                      >  1:1914928/1‑101 (MQ=255)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                                      <  2:162354/101‑1 (MQ=255)
                                                cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                                      <  1:1370187/101‑7 (MQ=2)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                     >  2:2759467/1‑101 (MQ=255)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                                     >  1:3488125/1‑94 (MQ=2)
                                                 tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                                     >  2:353153/1‑94 (MQ=2)
                                                  ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCACCGCGGGGGCGTGGATCGAAACCACCTTGc                                                    <  1:1030052/101‑1 (MQ=255)
                                                  ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAactgcgtgcg                                                    >  1:3209031/1‑93 (MQ=2)
                                                  ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcg                                                    >  1:2471703/1‑93 (MQ=2)
                                                  ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                                    >  1:3664895/1‑94 (MQ=2)
                                                  ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                                    <  1:143049/101‑9 (MQ=2)
                                                  ccccccGCGGGGGCGTCGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                                    <  2:1247086/101‑1 (MQ=255)
                                                   cccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgc                                                   >  2:3099463/1‑92 (MQ=2)
                                                   cccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcg                                                   <  2:3704360/101‑9 (MQ=0)
                                                    ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggct                                                  >  1:307041/1‑91 (MQ=2)
                                                    ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggct                                                  <  1:2672591/101‑11 (MQ=2)
                                                    ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGGGGATCGAAactatactggct                                                  >  1:1853914/1‑91 (MQ=2)
                                                      ccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                                                >  1:1543978/1‑101 (MQ=255)
                                                           ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                                           >  2:3388108/1‑101 (MQ=255)
                                                              gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                                        <  1:3272330/101‑1 (MQ=255)
                                                              gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                                        >  2:2448809/1‑101 (MQ=255)
                                                                gTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                      <  2:2597922/101‑1 (MQ=255)
                                                                gTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                      >  2:2672026/1‑101 (MQ=255)
                                                                gTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                      >  1:3570217/1‑101 (MQ=255)
                                                                 tGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                                     <  1:1068494/101‑1 (MQ=255)
                                                                 tGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                                     <  2:385895/101‑1 (MQ=255)
                                                                   gATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                                   <  1:3473716/101‑1 (MQ=255)
                                                                   gATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                                   >  1:4172500/1‑101 (MQ=255)
                                                                   gATCGAAACAGCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgcg                                   <  2:168255/101‑1 (MQ=255)
                                                                    aTCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCgg                                  >  1:4342196/1‑101 (MQ=255)
                                                                     tCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACCACCTTGCGGATTGTCACTTTCGCggg                                 >  1:490881/1‑101 (MQ=255)
                                                                     tCGAAACATCTCGGTGCCGCCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCCTTGCGGATTGTCACTTTCGCggg                                 <  1:2519850/101‑1 (MQ=255)
                                                                        aaaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                              <  2:2037602/101‑1 (MQ=255)
                                                                        aaaCATCCCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                              <  2:2882355/101‑1 (MQ=255)
                                                                         aaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa                             <  2:3835187/101‑1 (MQ=255)
                                                                           cATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                           <  1:4063769/101‑1 (MQ=255)
                                                                              ctcGGGGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACCACCTGGCGGATTGTCACTTTCGCGGGGCGAAGCGt                        >  1:298386/1‑101 (MQ=255)
                                                                               tcGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTc                       >  1:896966/1‑101 (MQ=255)
                                                                                cGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCg                      <  2:1439037/101‑1 (MQ=255)
                                                                                    gCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCCcc                  >  1:4317755/1‑101 (MQ=255)
                                                                                     ccGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccc                 <  1:1981295/101‑1 (MQ=255)
                                                                                      cGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccc                <  1:2089065/101‑1 (MQ=255)
                                                                                           cTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgcg           >  2:3318371/1‑101 (MQ=255)
                                                                                           cTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgcg           <  1:339808/101‑1 (MQ=255)
                                                                                            tCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgg          <  2:1049516/101‑1 (MQ=255)
                                                                                              g.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgggg        >  2:3989161/1‑101 (MQ=255)
                                                                                                   cAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCg     >  1:2897840/1‑101 (MQ=255)
                                                                                                      ccGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTgg  <  2:4127835/101‑1 (MQ=255)
                                                                                                      ccGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTgg  >  2:1199803/1‑101 (MQ=255)
                                                                                                      |                                                                                                      
GGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1395456‑1395656

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG