Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,557 C→T 12.6% intergenic (+369/‑562) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5570C→T12.6% 96.8 ‑1.8 ‑0.8308intergenic (+369/‑562)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/24):  ref base (139/130):  total (154/154)
Fisher's exact test strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.66e-01

Only 100 of 337 total aligned reads displayed.

GATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1395457‑1395657
                                                                                                     |                                                                                                      
gATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGcc                                                                                                        >  2:1921460/1‑101 (MQ=255)
    gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCCGCCGG.cc                                                                                                   >  1:1955854/1‑101 (MQ=255)
     aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCt                                                                                                  >  2:3734775/1‑101 (MQ=255)
     aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCt                                                                                                  >  1:158756/1‑101 (MQ=255)
       aCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.Gc                                                                                               >  1:3165648/1‑101 (MQ=255)
        cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCt                                                                                              <  1:2632478/101‑1 (MQ=255)
             ccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCtcgtcg                                                                                         <  2:4337967/101‑1 (MQ=255)
                     gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTcccccc                                                                                 <  2:1982857/101‑1 (MQ=255)
                      cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCcg                                                                                <  1:3530394/101‑1 (MQ=255)
                       ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCcgc                                                                               >  2:2642920/1‑101 (MQ=255)
                               ggCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGt                                                                       <  1:1704132/101‑1 (MQ=255)
                                gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTg                                                                      <  1:2781424/101‑1 (MQ=255)
                                gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTg                                                                      <  1:2742248/101‑1 (MQ=255)
                                 agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                                     <  2:2253123/91‑1 (MQ=0)
                                 agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                                     >  1:3113360/11‑101 (MQ=0)
                                   ggTAACGTTTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   <  1:3452692/101‑1 (MQ=255)
                                    gccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  1:2849624/94‑1 (MQ=2)
                                    gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  <  2:160625/101‑1 (MQ=255)
                                    gTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  >  2:1484180/1‑101 (MQ=255)
                                    ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  >  2:4065638/8‑101 (MQ=2)
                                    ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                  >  2:549093/8‑101 (MQ=2)
                                    ataccgcgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCA.ATCGGCCGG.GCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                   >  2:2542467/8‑101 (MQ=0)
                                     tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 >  1:1884232/7‑101 (MQ=2)
                                     tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 >  1:2408261/7‑101 (MQ=2)
                                     ccgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                 <  2:828281/95‑1 (MQ=2)
                                       ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               >  2:2115785/5‑101 (MQ=2)
                                       ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               >  1:3928751/5‑101 (MQ=2)
                                       gcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                               >  2:3574842/5‑101 (MQ=2)
                                        ccggtCGCGCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCGGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                              <  2:3042668/98‑1 (MQ=2)
                                        cacgtCGCTCCCCCCGTGGGGGCTTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                              <  1:2814487/98‑1 (MQ=2)
                                        cacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                              >  2:1786142/4‑101 (MQ=2)
                                        cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                              >  2:3851969/1‑101 (MQ=255)
                                         ctgccccTCCCCCCGCGGGGGCGTGGATCCAAACACCTCGGTGCCGCCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                             <  2:4212068/95‑1 (MQ=37)
                                          tgtCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                            <  1:1130060/101‑1 (MQ=255)
                                          ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                            >  1:2571264/2‑101 (MQ=2)
                                          ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                            <  1:1300839/100‑2 (MQ=2)
                                          cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaat                                                            >  2:70497/2‑99 (MQ=2)
                                           gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactg                                                           <  2:1013000/101‑3 (MQ=2)
                                           gtCGCCCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccc                                                           <  1:879407/101‑2 (MQ=2)
                                           gccGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacca                                                           <  2:1729345/99‑1 (MQ=255)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                                          >  2:3636307/1‑101 (MQ=255)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccac                                                          >  2:2982962/1‑101 (MQ=255)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacccc                                                          <  2:2147390/101‑3 (MQ=2)
                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactat                                                          <  2:486905/101‑4 (MQ=2)
                                            gcGCCCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                                          <  2:1132644/100‑4 (MQ=2)
                                            gcGCCCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                                          <  2:1362712/100‑4 (MQ=2)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                                         >  1:445343/1‑101 (MQ=255)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                                         >  1:4141306/1‑101 (MQ=255)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                                         >  2:2681488/1‑101 (MQ=255)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccg                                                         <  1:1774709/101‑4 (MQ=2)
                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                         <  2:3549457/101‑5 (MQ=2)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgt                                                        >  1:2376134/1‑96 (MQ=2)
                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgc                                                        <  1:4034304/101‑6 (MQ=2)
                                               cgccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                                       <  2:1316548/99‑7 (MQ=2)
                                               cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                                       <  2:162354/101‑1 (MQ=255)
                                               cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                                       <  1:1370187/101‑7 (MQ=2)
                                                tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                      >  2:2759467/1‑101 (MQ=255)
                                                tCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgc                                                      >  1:3488125/1‑94 (MQ=2)
                                                cccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                      <  1:4338274/100‑1 (MQ=255)
                                                 ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                                     >  1:4202298/1‑101 (MQ=255)
                                                 ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCACCGCGGGGGCGTGGATCGAAACCACCTTGc                                                     <  1:1030052/101‑1 (MQ=255)
                                                 ccccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcg                                                     >  1:2471703/1‑93 (MQ=2)
                                                 ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                                     >  1:3664895/1‑94 (MQ=2)
                                                 ccccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctgg                                                     <  1:143049/101‑9 (MQ=2)
                                                  cccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgc                                                    >  2:3099463/1‑92 (MQ=2)
                                                  cccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcg                                                    >  2:4161767/1‑93 (MQ=0)
                                                  cccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCGGCAACCGG.CCC.G.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggcg                                                    <  2:3704360/101‑9 (MQ=0)
                                                   ccccGCGGGGGCGTTGATCGAAACAATGCGGCGCGGGC.CTAT.GGCTGACGGACCC.GCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgcg                                                   <  1:1869934/101‑11 (MQ=0)
                                                   ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.TGCGTCTGGAACA.GGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggct                                                   <  1:2672591/101‑11 (MQ=2)
                                                     ccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAt                                                 >  1:1543978/1‑101 (MQ=255)
                                                      cgcgGGGGGGTGGATCGAAACATCTCGGTGCCGTCGCCCC.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                                <  1:4301499/101‑1 (MQ=255)
                                                      cgcgGGGGCGTGGATCGAAACCTCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAtt                                                <  1:693056/101‑1 (MQ=255)
                                                        cgGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGt                                              <  1:3184118/101‑1 (MQ=255)
                                                         gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTc                                             >  1:997113/1‑101 (MQ=255)
                                                         gggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCCAAAACACCTTGCGGATTGTc                                             >  2:1265556/1‑101 (MQ=255)
                                                          ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                                            >  2:3388108/1‑101 (MQ=255)
                                                           gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                                           >  1:4078246/1‑101 (MQ=255)
                                                           gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGAATGTCAc                                           >  1:514874/1‑101 (MQ=255)
                                                            ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                                          >  1:1667766/1‑101 (MQ=255)
                                                             gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                                         <  1:3272330/101‑1 (MQ=255)
                                                             gCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACtt                                         >  2:2448809/1‑101 (MQ=255)
                                                              cGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACttt                                        <  1:816256/101‑1 (MQ=255)
                                                               gTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                       <  2:2597922/101‑1 (MQ=255)
                                                                tGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                                      <  1:1068494/101‑1 (MQ=255)
                                                                 ggATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcgc                                     >  1:3064455/1‑101 (MQ=255)
                                                                    tCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCggg                                  <  2:2368633/101‑1 (MQ=255)
                                                                    tCGAAACATCTCGGTGCCGCCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCCTTGCGGATTGTCACTTTCGCggg                                  <  1:2519850/101‑1 (MQ=255)
                                                                       aaaCATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                               <  2:2037602/101‑1 (MQ=255)
                                                                       aaaCATCCCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                               <  2:2882355/101‑1 (MQ=255)
                                                                          cATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAGCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                            >  1:3789093/1‑101 (MQ=255)
                                                                             ctcGGGGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACCACCTGGCGGATTGTCACTTTCGCGGGGCGAAGCGt                         >  1:298386/1‑101 (MQ=255)
                                                                                     cGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccc                 >  2:3910437/1‑101 (MQ=255)
                                                                                       tCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTcccccc               <  1:2051075/101‑1 (MQ=255)
                                                                                          cTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgcg            <  1:339808/101‑1 (MQ=255)
                                                                                          cTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCcgcg            >  2:3318371/1‑101 (MQ=255)
                                                                                           tCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgg           <  1:2208265/101‑1 (MQ=255)
                                                                                           tCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgg           <  2:1049516/101‑1 (MQ=255)
                                                                                                    ccGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTgg   <  2:4127835/101‑1 (MQ=255)
                                                                                                    ccGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTgg   >  2:1199803/1‑101 (MQ=255)
                                                                                                     cGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGa  >  1:1576252/1‑101 (MQ=255)
                                                                                                     |                                                                                                      
GATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.TCCAGCCGG.CCT.GCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGA  >  NC_014034/1395457‑1395657

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG