BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,675	A→T	5.6%	intergenic (+487/‑444)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,675	0	A→T	5.6%	25.8 ‑1.0 ‑0.6	216	intergenic (+487/‑444)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (8/4): ref base (102/101): total (110/106)
Fisher's exact test strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.67e-01

Only 100 of 246 total aligned reads displayed.


CCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCC  >  NC_014034/1395575‑1395775


ccccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCCTGTa                                                                                                         >  2:4003822/1‑101 (MQ=255)


 cccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAg                                                                                                        <  1:2772661/101‑1 (MQ=255)


 cccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAg                                                                                                        <  1:618687/101‑1 (MQ=255)


  ccGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGt                                                                                                       <  1:3693159/101‑1 (MQ=255)


   cgcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCTGCATGTAGTc                                                                                                      >  1:954095/1‑101 (MQ=255)


   cgcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTc                                                                                                      <  1:228663/101‑1 (MQ=255)


    gcgGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcg                                                                                                     <  2:1715516/101‑1 (MQ=255)


      gggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggc                                                                                                   >  2:32946/1‑101 (MQ=255)


       ggggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggcg                                                                                                  >  1:68600/1‑101 (MQ=255)


         ggCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggcg.gc                                                                                               <  1:2572949/101‑2 (MQ=255)


           cGTGGATCGAAACCACCTTGCGGATTGTCAGTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCa                                                                                            >  1:3974390/1‑101 (MQ=255)


           cGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCa                                                                                            >  1:2453312/1‑101 (MQ=255)


            gTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAg                                                                                           >  2:406220/1‑101 (MQ=255)


            gTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAg                                                                                           >  2:2535801/1‑101 (MQ=255)


             tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGGC.G.GCact                                                                                          >  1:453205/1‑99 (MQ=255)


             tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGt                                                                                          >  1:3747002/1‑101 (MQ=255)


             tGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGt                                                                                          >  2:702663/1‑101 (MQ=255)


             gggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGt                                                                                          <  1:3098369/100‑1 (MQ=255)


              ggATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGtt                                                                                         >  1:1837522/1‑101 (MQ=255)


               gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTcggcgccttttgc                                                                                          <  2:1667655/101‑9 (MQ=255)


               gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTc                                                                                        <  1:3318603/101‑1 (MQ=255)


               gATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTc                                                                                        >  1:351398/1‑101 (MQ=255)


                 gcGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGa                                                                                      <  2:1487304/100‑1 (MQ=255)


                  cGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.g                                                                                    >  1:2237007/1‑101 (MQ=255)


                   gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.Gc                                                                                   >  2:3483763/1‑101 (MQ=255)


                    aaaCCGCCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGTG.G.GCAGTTCGA.Gcc                                                                                  <  2:2317702/101‑1 (MQ=255)


                    aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.Gcc                                                                                  <  1:3050159/101‑1 (MQ=255)


                     aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCg                                                                                 >  2:3735689/1‑101 (MQ=255)


                     aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCg                                                                                 <  1:741645/101‑1 (MQ=255)


                       cccccTTGCGGATTGTCCCTTTCGCGGGGCGAAGCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCa                                                                               <  1:1325651/98‑1 (MQ=255)


                       cccccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCa                                                                               <  2:490829/98‑1 (MQ=255)


                       ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCa                                                                               <  2:194520/101‑1 (MQ=255)


                       ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCa                                                                               >  2:1269782/1‑101 (MQ=255)


                       ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCa                                                                               <  2:2897632/101‑1 (MQ=255)


                         accTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGt                                                                             <  1:1040768/101‑1 (MQ=255)


                           cTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCg                                                                           >  1:578040/1‑101 (MQ=255)


                            ttGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGc                                                                          <  1:2643091/101‑1 (MQ=255)


                             tGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCt                                                                         <  1:3440399/101‑1 (MQ=255)


                              gCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTc                                                                        >  2:594554/1‑101 (MQ=255)


                               cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTcc                                                                       >  1:4014569/1‑101 (MQ=255)


                                 gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTcccc                                                                     >  2:4329207/1‑101 (MQ=255)


                                 gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTcccc                                                                     <  1:427003/101‑1 (MQ=255)


                                 gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTcccc                                                                     <  2:3064234/101‑1 (MQ=255)


                                    tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                                  <  1:3636560/101‑1 (MQ=255)


                                      tCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCcgcg                                                                <  1:500662/101‑1 (MQ=255)


                                       cccTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgg                                                               <  2:4054374/99‑1 (MQ=255)


                                       cACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgg                                                               <  1:3878685/101‑1 (MQ=255)


                                       cACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgg                                                               <  1:2981603/101‑1 (MQ=255)


                                          tttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTACCCCCGCggggg                                                            >  2:2015694/1‑101 (MQ=255)


                                           ttCGCGGGTCGAAGCGTCGCCCCCCCCGCGGGGACGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGc                                                           <  1:3852222/101‑1 (MQ=255)


                                              gcgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTg                                                        <  2:2236862/101‑1 (MQ=255)


                                               gggggCGACGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTgg                                                       <  1:1265649/100‑1 (MQ=255)


                                               cgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTgg                                                       <  2:2745645/101‑1 (MQ=255)


                                                ggggCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGGGGa                                                      >  1:1943772/1‑101 (MQ=255)


                                                 gggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                     <  2:3974134/101‑1 (MQ=255)


                                                 gggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                     <  2:896881/101‑1 (MQ=255)


                                                  gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                     >  2:3392779/7‑101 (MQ=2)


                                                   gCGAGGCGTCCCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  2:1581173/101‑1 (MQ=255)


                                                   gCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  1:1096445/101‑1 (MQ=255)


                                                   gCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                   <  1:2359705/101‑1 (MQ=255)


                                                    cGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                  <  2:4172239/101‑1 (MQ=255)


                                                     gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                                 >  1:1869936/1‑101 (MQ=255)


                                                     cgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCCC.GTCGCCGTCGGTCCCCCCGCGGGGGCGTGGATCGa                                                  <  2:154366/96‑1 (MQ=2)


                                                      aaGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                >  2:472437/1‑101 (MQ=255)


                                                        gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                               >  1:217397/1‑101 (MQ=2)


                                                         cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                             >  1:311163/1‑101 (MQ=255)


                                                             gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTAt                                         >  2:2973063/1‑101 (MQ=255)


                                                              cTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATg                                        <  2:1071177/101‑1 (MQ=255)


                                                               tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                       >  1:4068018/1‑101 (MQ=255)


                                                               tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                       >  2:3225306/1‑101 (MQ=255)


                                                               tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                       >  2:356006/1‑101 (MQ=255)


                                                                ccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                                      <  2:1389129/101‑1 (MQ=255)


                                                                  ccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATc                                    >  1:1445663/1‑101 (MQ=255)


                                                                      gcgGGGGCGTGGATCGAAACATCTGCAGGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                <  2:1837398/101‑1 (MQ=255)


                                                                         ggggCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCa                             <  2:3908662/101‑1 (MQ=255)


                                                                           ggCGTGGATCGAAACATCTGCATGTAGTCGGGG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaaa                           >  1:3951700/1‑101 (MQ=255)


                                                                            gCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAAc                          >  1:3368259/1‑101 (MQ=255)


                                                                             ggTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                         <  2:4245022/100‑1 (MQ=255)


                                                                             cGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                         >  1:2711974/1‑101 (MQ=255)


                                                                             cGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                         >  1:602825/1‑101 (MQ=255)


                                                                              gtagATGGAAACATATGCATGTGGCCGGGG.G.GGAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                        <  1:1193797/98‑1 (MQ=25)


                                                                              gTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                        >  1:2054589/1‑101 (MQ=255)


                                                                              gTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                        >  2:1547835/1‑101 (MQ=255)


                                                                               tGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                       <  2:1743662/101‑1 (MQ=255)


                                                                               tGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                       >  2:829063/1‑101 (MQ=255)


                                                                                  aTCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg                    >  1:2134865/1‑101 (MQ=255)


                                                                                  aTCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg                    >  1:80252/1‑101 (MQ=255)


                                                                                      aaaCATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTg                <  2:3165424/101‑1 (MQ=255)


                                                                                        aCACCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtc              <  2:2702594/101‑1 (MQ=255)


                                                                                         cATCTGCATGTAGTCGGGG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcg             <  1:3230413/101‑1 (MQ=255)


                                                                                          ctcggCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt            <  2:4141045/97‑1 (MQ=255)


                                                                                          aTCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt            >  2:1172522/1‑101 (MQ=255)


                                                                                           tCTGCATGTGGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc           <  1:2672198/101‑1 (MQ=255)


                                                                                           tCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc           <  2:2453155/101‑1 (MQ=255)


                                                                                              tgaTGTAGGGGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt        <  1:1000128/99‑1 (MQ=255)


                                                                                                  gTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc    <  2:2937660/101‑1 (MQ=255)


                                                                                                    aGTCGGGG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc  <  1:1415543/101‑1 (MQ=255)


                                                                                                    aGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc  <  1:1719863/101‑1 (MQ=255)


                                                                                                    aGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc  >  1:2032090/1‑101 (MQ=255)


                                                                                                    aGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc  >  1:2850836/1‑101 (MQ=255)


CCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCC  >  NC_014034/1395575‑1395775

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG