Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,693 G→A 5.6% intergenic (+505/‑426) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,6930G→A 5.6% 20.2 ‑1.4 ‑0.0215intergenic (+505/‑426)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (8/4):  ref base (98/105):  total (106/109)
Fisher's exact test strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.91e-01

Only 100 of 236 total aligned reads displayed.

CGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395593‑1395793
                                                                                                       |                                                                                                    
cGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.g                                                                                                      <  1:2218143/101‑1 (MQ=255)
 gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.Gc                                                                                                     >  1:3982282/1‑101 (MQ=255)
 gAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.Gc                                                                                                     >  2:3483763/1‑101 (MQ=255)
  aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.Gcc                                                                                                    >  1:3094899/1‑101 (MQ=255)
  aaaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.Gcc                                                                                                    >  2:2835884/1‑101 (MQ=255)
   aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCg                                                                                                   >  2:3735689/1‑101 (MQ=255)
   aaCCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCg                                                                                                   <  1:741645/101‑1 (MQ=255)
     ccaccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCa                                                                                                 <  2:2897632/101‑1 (MQ=255)
      caccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAg                                                                                                >  2:621506/1‑101 (MQ=255)
       accTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGt                                                                                               <  1:1040768/101‑1 (MQ=255)
        ccTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTc                                                                                              <  1:2441117/101‑1 (MQ=255)
           tGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCt                                                                                           <  1:3440399/101‑1 (MQ=255)
             cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTcc                                                                                         >  1:4014569/1‑101 (MQ=255)
             cGGATTGTCACTTTCGCGGGGCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTcc                                                                                         <  1:3713683/101‑1 (MQ=255)
               gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTcccc                                                                                       <  1:427003/101‑1 (MQ=255)
               gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTcccc                                                                                       >  1:2606808/1‑101 (MQ=255)
               gATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTcccc                                                                                       <  2:3064234/101‑1 (MQ=255)
                aTTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTccccc                                                                                      <  1:3627139/101‑1 (MQ=255)
                  tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                                                    <  1:3636560/101‑1 (MQ=255)
                  tGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCcg                                                                                    >  1:829358/1‑101 (MQ=255)
                      aCTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCggg                                                                                >  1:1931550/1‑101 (MQ=255)
                       cTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCgggg                                                                               <  1:2352298/101‑1 (MQ=255)
                         ttCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGc                                                                             <  2:3951089/101‑1 (MQ=255)
                           cgcgGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCgg                                                                           >  2:4280485/1‑100 (MQ=255)
                            tggtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGt                                                                           >  1:3853430/11‑101 (MQ=2)
                             gggggCGACGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  1:1265649/100‑1 (MQ=255)
                              gtgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  2:489962/93‑1 (MQ=2)
                               tgccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                        >  2:1717133/8‑101 (MQ=0)
                               gggCGAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  2:3264323/101‑1 (MQ=255)
                                ggCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      >  1:545258/1‑101 (MQ=255)
                                gccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       >  2:3392779/7‑101 (MQ=2)
                                 gCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     <  1:1096445/101‑1 (MQ=255)
                                 ccgctgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  2:3976626/96‑1 (MQ=2)
                                  cGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    <  2:4172239/101‑1 (MQ=255)
                                  cGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    >  1:2300615/1‑101 (MQ=255)
                                   gAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                                                   >  1:1869936/1‑101 (MQ=255)
                                   gAAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   <  1:2983183/101‑1 (MQ=255)
                                   cgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAA.GCTGGTCCC.GTCGCCGTCGGTCCCCCCGCGGGGGCGTGGATCGa                                                                    <  2:154366/96‑1 (MQ=2)
                                    aaGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  >  2:4204227/1‑101 (MQ=255)
                                    aaGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  >  2:4071815/1‑101 (MQ=255)
                                     tgCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  >  2:2212590/2‑101 (MQ=2)
                                     aGCGTCGGCCCCCCCGGGGGGGCGTGGATCGAAACAGCTGCATGTAGTGGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 <  2:2459554/101‑1 (MQ=37)
                                      gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 >  1:217397/1‑101 (MQ=2)
                                      gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGGCG.GCGAACACCGATACCGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 <  2:4005160/101‑1 (MQ=2)
                                       cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                               >  1:311163/1‑101 (MQ=255)
                                       cGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                               <  2:1576146/101‑1 (MQ=255)
                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgg                                                              <  2:3788204/101‑1 (MQ=255)
                                         ccGCCCCCCCCGGGGGGGGGTGGTTCGAAATATCTCCATGTAGCCGGCG.G.GCAGTCCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGt                                                             <  2:1802287/100‑1 (MQ=25)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTAt                                                           >  2:2973063/1‑101 (MQ=255)
                                            cTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATg                                                          <  2:1071177/101‑1 (MQ=255)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATgg                                                         >  2:356006/1‑101 (MQ=255)
                                              ccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGa                                                        <  2:1389129/101‑1 (MQ=255)
                                                    gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgc                                                  >  2:4365631/1‑101 (MQ=255)
                                                     cgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcg                                                 >  1:328819/1‑101 (MQ=255)
                                                     cgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcg                                                 >  1:2722511/1‑101 (MQ=255)
                                                      gggggCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGgcgc                                                >  2:771128/1‑101 (MQ=255)
                                                      gggggCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACTGGTATGGATCTGgcgc                                                >  2:562734/1‑101 (MQ=255)
                                                         ggCGTGGATCGAAACATCTGCATGTAGTCGGGG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaaa                                             >  1:3951700/1‑101 (MQ=255)
                                                         ggCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCaaa                                             <  1:3362370/101‑1 (MQ=255)
                                                           cGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                                           >  1:602825/1‑101 (MQ=255)
                                                           cGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACg                                           >  1:2661199/1‑101 (MQ=255)
                                                            gtagATGGAAACATATGCATGTGGCCGGGG.G.GGAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                          <  1:1193797/98‑1 (MQ=25)
                                                            gTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACgg                                          >  1:2054589/1‑101 (MQ=255)
                                                             tGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                         <  2:1743662/101‑1 (MQ=255)
                                                             tGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                         >  2:229294/1‑101 (MQ=255)
                                                             tGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGa                                         >  2:1281276/1‑101 (MQ=255)
                                                                aTCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAg                                      >  1:2134865/1‑101 (MQ=255)
                                                                  cGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTAGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTc                                    <  1:594622/101‑1 (MQ=255)
                                                                   gAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                                   <  2:1869812/101‑1 (MQ=255)
                                                                   gAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                                   >  1:1438693/1‑101 (MQ=255)
                                                                   gAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCt                                   >  1:1306888/1‑101 (MQ=255)
                                                                        aTCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgt                              >  2:1172522/1‑101 (MQ=255)
                                                                         tCTGCATGTGGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                             <  1:2672198/101‑1 (MQ=255)
                                                                         tCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTgtcgtc                             <  1:3172250/101‑1 (MQ=255)
                                                                           tGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGc                           >  1:1766536/1‑101 (MQ=255)
                                                                            tgaTGTAGGGGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt                          <  1:1000128/99‑1 (MQ=255)
                                                                            gCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCt                          >  2:1950133/1‑101 (MQ=255)
                                                                               tGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTccc                       <  1:3039365/101‑1 (MQ=255)
                                                                                gTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc                      <  1:3388345/101‑1 (MQ=255)
                                                                                gTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccc                      >  2:1625582/1‑101 (MQ=255)
                                                                                  aGTCGGGG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc                    <  1:1415543/101‑1 (MQ=255)
                                                                                  aGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTcccccc                    >  1:2032090/1‑101 (MQ=255)
                                                                                   gTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTccccccc                   >  2:1499411/1‑100 (MQ=255)
                                                                                   aattcgaagagCAGTACGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTAGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTccccc                     <  2:2789949/91‑1 (MQ=37)
                                                                                      ggcg.g.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCgg                >  2:3033081/1‑101 (MQ=255)
                                                                                       gcg.g.GCAGTTGGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCggg               <  2:311128/101‑1 (MQ=255)
                                                                                               aGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTg         >  1:3816219/1‑101 (MQ=255)
                                                                                                gtgcGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTgg        <  2:1927522/98‑1 (MQ=255)
                                                                                                  tCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGAt      >  1:2790740/1‑101 (MQ=255)
                                                                                                  tCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGAt      >  1:1547618/1‑101 (MQ=255)
                                                                                                   cGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATc     <  2:829276/101‑1 (MQ=255)
                                                                                                   cGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATc     >  2:3196943/1‑101 (MQ=255)
                                                                                                    gA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg    <  1:771208/101‑1 (MQ=255)
                                                                                                    gA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCg    >  2:4114243/1‑101 (MQ=255)
                                                                                                     a.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGa   >  2:2588555/1‑101 (MQ=255)
                                                                                                       gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  2:445303/101‑1 (MQ=255)
                                                                                                       gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  >  1:4084272/1‑101 (MQ=255)
                                                                                                       gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  1:4329476/101‑1 (MQ=255)
                                                                                                       gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  2:2722321/101‑1 (MQ=255)
                                                                                                       gCCGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  2:883851/101‑1 (MQ=255)
                                                                                                       |                                                                                                    
CGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCG.G.GCAGTTCGA.GCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGGTATGGATCTGGCGCAAACGGAAAGTCTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1395593‑1395793

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG