Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,732 G→T 10.4% intergenic (+544/‑387) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,7320G→T10.4% 74.7 ‑2.8 0.0293intergenic (+544/‑387)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/21):  ref base (132/130):  total (142/151)
Fisher's exact test strand distribution p-value = 6.01e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACG  >  NC_014034/1395632‑1395832
                                                                                                     |                                                                                                           
cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.g                                                                                                             >  1:311163/1‑101 (MQ=255)
  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGt                                                                                                           <  1:1269875/101‑1 (MQ=255)
  gcGCCCCCCCCGGCGGGGGGTGGATCAACACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGt                                                                                                           <  1:32959/100‑1 (MQ=25)
     ccccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATg                                                                                                        <  1:217975/99‑1 (MQ=255)
     cTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATg                                                                                                        <  2:1071177/101‑1 (MQ=255)
     cTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATg                                                                                                        >  2:3039144/1‑101 (MQ=255)
         ccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATc                                                                                                   >  1:1445663/1‑101 (MQ=255)
          cccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCt                                                                                                  <  1:1385603/101‑1 (MQ=255)
           ccGCGGGGGCGTGGATCGAAACATCTGCATGGAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTg                                                                                                 <  1:4278723/101‑1 (MQ=255)
             gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..gc                                                                                             >  2:4365631/1‑101 (MQ=255)
               gggggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..gcgc                                                                                           >  2:771128/1‑101 (MQ=255)
                  ggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCaaa                                                                                        <  2:1531056/101‑1 (MQ=255)
                   gCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAAc                                                                                       <  1:2661211/101‑1 (MQ=255)
                   gCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAAc                                                                                       >  1:3368259/1‑101 (MQ=255)
                     gTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACgg                                                                                     >  1:3768994/1‑101 (MQ=255)
                      tGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..a                                                                                  >  2:829063/1‑101 (MQ=255)
                      tGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..a                                                                                  >  2:1281276/1‑101 (MQ=255)
                         aTCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAg                                                                               >  2:3603562/1‑101 (MQ=255)
                         aTCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAg                                                                               >  1:80252/1‑101 (MQ=255)
                            gAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCt                                                                            <  2:1869812/101‑1 (MQ=255)
                            gAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCt                                                                            >  1:1306888/1‑101 (MQ=255)
                             aaaCATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g                                                                          <  2:3165424/101‑1 (MQ=255)
                               aCACCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g.tc                                                                       <  2:2702594/101‑1 (MQ=255)
                                cATCTGCATGTAGTCGGCGGGCAGTTCGAGCAGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g.tcg                                                                      >  1:3958474/1‑101 (MQ=255)
                                 ctcggCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g.tcgt                                                                     <  2:4141045/97‑1 (MQ=255)
                                 aTCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g.tcgt                                                                     >  2:1172522/1‑101 (MQ=255)
                                     tgaTGTAGGGGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCt                                                                 <  1:1000128/99‑1 (MQ=255)
                                     gcctGTAGGCGGCGGGCAGTGCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCt                                                                 <  1:2262801/98‑1 (MQ=255)
                                        tGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTccc                                                              <  1:3039365/101‑1 (MQ=255)
                                         gTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccc                                                             >  2:1625582/1‑101 (MQ=255)
                                           aGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccccc                                                           >  1:2032090/1‑101 (MQ=255)
                                             tCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGGATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCcgc                                                         >  2:509465/1‑101 (MQ=255)
                                                   ggCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGc                                                   <  2:2528726/101‑1 (MQ=255)
                                                         tcaagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTg                                                >  1:2844909/9‑101 (MQ=2)
                                                         tcaagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTg                                                <  2:1696781/93‑1 (MQ=2)
                                                          caagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTgg                                               <  2:856392/94‑1 (MQ=2)
                                                          caagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGACG.G.TGGTCGCTCCCCCCGCGGGGGCGTgg                                               >  2:1136513/8‑101 (MQ=2)
                                                          cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATc                                            >  2:3196943/1‑101 (MQ=255)
                                                          cGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCCCGGGGGCGTGGATc                                            >  1:1668730/1‑101 (MQ=255)
                                                           gcgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:1048791/93‑1 (MQ=0)
                                                           gcgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                             >  1:1433172/9‑101 (MQ=0)
                                                           aagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGa                                              <  1:2062223/95‑1 (MQ=2)
                                                            aGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          >  2:2588555/1‑101 (MQ=255)
                                                             gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  1:268709/95‑1 (MQ=0)
                                                             gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  1:4329476/101‑1 (MQ=255)
                                                             gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         >  1:4084272/1‑101 (MQ=255)
                                                              cgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          <  1:3879924/96‑1 (MQ=2)
                                                               ttcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          >  1:2736164/3‑101 (MQ=2)
                                                               cGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       >  2:1798745/1‑101 (MQ=255)
                                                               cGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       <  1:406260/101‑1 (MQ=255)
                                                                 tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       >  1:692998/3‑101 (MQ=2)
                                                                 cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                     <  2:1943643/101‑1 (MQ=255)
                                                                 cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                     >  1:1203010/1‑101 (MQ=255)
                                                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                      <  1:3510966/100‑1 (MQ=2)
                                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                   <  1:921768/101‑1 (MQ=255)
                                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGCGGGCGTGGATCGAAACCTgcg                                  <  1:2535964/101‑1 (MQ=255)
                                                                    gcGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCta                                    <  1:583479/100‑2 (MQ=2)
                                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAAcacc                                    >  2:2742486/1‑98 (MQ=2)
                                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.ATGACCAGTT..CCGCAATCGG..CCGGGCTCGATCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                   >  1:762208/1‑99 (MQ=2)
                                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                   <  1:523838/101‑3 (MQ=2)
                                                                      gCTCGCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                   >  2:3764467/1‑97 (MQ=0)
                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                   >  2:1468537/1‑97 (MQ=2)
                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                   <  1:1122108/101‑5 (MQ=2)
                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATATG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcggc                                >  2:3221327/1‑101 (MQ=255)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                  >  1:1755366/1‑96 (MQ=2)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                  <  2:1755247/101‑6 (MQ=2)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                  <  2:642519/101‑6 (MQ=2)
                                                                        tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                >  2:2805164/1‑96 (MQ=2)
                                                                        tCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGt                              >  2:3096836/1‑101 (MQ=255)
                                                                         ccccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                             <  1:2865686/101‑1 (MQ=255)
                                                                         ccccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                             <  2:1445556/101‑1 (MQ=255)
                                                                          cccccGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcg                               >  1:608148/1‑93 (MQ=2)
                                                                          cccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGa                            >  1:1825055/1‑101 (MQ=255)
                                                                           ccccGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgc                              >  1:3143641/1‑92 (MQ=2)
                                                                            cccGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGTGCGTGGATCGAAAcacccggcgcc                             <  1:4415426/101‑11 (MQ=2)
                                                                            cccGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgcc                             <  2:2623561/101‑11 (MQ=2)
                                                                            cccGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgcc                             <  2:396788/101‑11 (MQ=2)
                                                                            cccGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgcc                             <  2:3716852/101‑11 (MQ=2)
                                                                            cccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGt                          <  2:2987489/101‑1 (MQ=255)
                                                                               gcgGGGGCGTGGATCGAAACT.GGTATG.GATCCG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGc                       <  2:4089459/101‑1 (MQ=255)
                                                                                cgGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTGGcc                      >  2:2943048/1‑101 (MQ=255)
                                                                                cgGGGGCGTGGATCGAAACT.GGTATG.GATATG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGcc                      <  1:3221556/101‑1 (MQ=255)
                                                                                 gggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                     >  2:445663/1‑101 (MQ=255)
                                                                                 gggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                     >  2:4292067/1‑101 (MQ=255)
                                                                                 gggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                     >  1:1904105/1‑101 (MQ=255)
                                                                                  ggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                    <  2:3788840/101‑1 (MQ=255)
                                                                                  ggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                    <  2:2850629/101‑1 (MQ=255)
                                                                                      cGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGAt                >  2:2086476/1‑101 (MQ=255)
                                                                                        tGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg              <  2:3439032/101‑1 (MQ=255)
                                                                                        tGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg              <  2:157590/101‑1 (MQ=255)
                                                                                        tGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg              <  1:2578805/101‑1 (MQ=255)
                                                                                           aTCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg           <  2:1914804/101‑1 (MQ=255)
                                                                                           aTCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg           >  1:1654518/1‑101 (MQ=255)
                                                                                           aTCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg           >  1:4153850/1‑101 (MQ=255)
                                                                                            tCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGc          <  2:4067758/101‑1 (MQ=255)
                                                                                             cGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGcc         >  2:1343408/1‑101 (MQ=255)
                                                                                                aaCT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTg      >  2:3314314/1‑101 (MQ=255)
                                                                                                aaCT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTg      >  1:2305908/1‑101 (MQ=255)
                                                                                                  cT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa    <  1:1146380/101‑1 (MQ=255)
                                                                                                   t.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGAc   <  1:3097060/101‑1 (MQ=255)
                                                                                                     |                                                                                                           
CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACG  >  NC_014034/1395632‑1395832

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG