Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,736 T→A 15.2% intergenic (+548/‑383) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,7360T→A15.2% 133.0 ‑1.1 ‑0.5296intergenic (+548/‑383)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/26):  ref base (127/124):  total (146/150)
Fisher's exact test strand distribution p-value = 3.34e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.53e-01

Only 100 of 304 total aligned reads displayed.

GCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGC  >  NC_014034/1395636‑1395836
                                                                                                     |                                                                                                           
gCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTAt                                                                                                             >  2:2973063/1‑101 (MQ=255)
 ccccccccGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATg                                                                                                            <  1:217975/99‑1 (MQ=255)
 cTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATg                                                                                                            <  1:2681678/101‑1 (MQ=255)
  tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATg.g                                                                                                          >  2:2939734/1‑101 (MQ=255)
  tCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATg.g                                                                                                          >  2:356006/1‑101 (MQ=255)
      cccGGGGGGGCGTGGATCGAAACATCTGCATGTAGTGGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCt                                                                                                      <  1:2566959/101‑1 (MQ=255)
       ccGCGGGGGCGTGGATCGAAACATCTGCATGGAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTg                                                                                                     <  1:4278723/101‑1 (MQ=255)
         gcgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..gc                                                                                                 >  2:4365631/1‑101 (MQ=255)
          cgGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..gcg                                                                                                >  1:2722511/1‑101 (MQ=255)
           gggggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..gcgc                                                                                               >  2:771128/1‑101 (MQ=255)
             gggCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCaa                                                                                             <  2:282795/101‑1 (MQ=255)
                cGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACg                                                                                          >  1:2661199/1‑101 (MQ=255)
                 gTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACgg                                                                                         >  1:3768994/1‑101 (MQ=255)
                 gTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACgg                                                                                         >  2:1547835/1‑101 (MQ=255)
                 gTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACgg                                                                                         >  1:2037351/1‑101 (MQ=255)
                  tGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..a                                                                                      >  2:829063/1‑101 (MQ=255)
                     aTCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAg                                                                                   >  1:80252/1‑101 (MQ=255)
                      tCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGt                                                                                  <  2:321850/101‑1 (MQ=255)
                        gAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCt                                                                                <  2:1869812/101‑1 (MQ=255)
                            cATCTGCATGTAGTCGGCGGGCAGTTCGAGCAGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g.tcg                                                                          >  1:3958474/1‑101 (MQ=255)
                             ctcggCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g.tcgt                                                                         <  2:4141045/97‑1 (MQ=255)
                              tCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g.tcgtc                                                                        <  1:3172250/101‑1 (MQ=255)
                              tCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.g.tcgtc                                                                        <  2:2453155/101‑1 (MQ=255)
                                tGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGc                                                                      >  1:1766536/1‑101 (MQ=255)
                                 tgaTGTAGGGGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCt                                                                     <  1:1000128/99‑1 (MQ=255)
                                    tGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTccc                                                                  <  1:3039365/101‑1 (MQ=255)
                                     gTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccc                                                                 >  2:1625582/1‑101 (MQ=255)
                                     gTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccc                                                                 <  1:3388345/101‑1 (MQ=255)
                                     gTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTcccc                                                                 >  2:1301508/1‑101 (MQ=255)
                                      aattcgaagagCAGTACGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTAGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTccccc                                                                <  2:2789949/91‑1 (MQ=37)
                                         tCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGGATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCcgc                                                             >  2:509465/1‑101 (MQ=255)
                                           ggcggGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCgg                                                           >  2:3033081/1‑101 (MQ=255)
                                            gcggGCAGTTGGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCggg                                                          <  2:311128/101‑1 (MQ=255)
                                                 gaGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGt                                                     <  2:1795476/100‑1 (MQ=255)
                                                  aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGTTCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTg                                                    >  1:2071340/1‑101 (MQ=255)
                                                  aGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCTTg                                                    >  1:335673/1‑101 (MQ=255)
                                                     tcaagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTg                                                    >  1:2844909/9‑101 (MQ=2)
                                                     tcaagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTg                                                    <  2:1696781/93‑1 (MQ=2)
                                                      gccgggctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGa                                                  >  2:330869/10‑101 (MQ=0)
                                                      caagattcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTgg                                                   <  2:856392/94‑1 (MQ=2)
                                                       gcgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                 <  1:1048791/93‑1 (MQ=0)
                                                         gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  1:861278/95‑1 (MQ=0)
                                                         gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             >  1:4084272/1‑101 (MQ=255)
                                                         gCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  1:4329476/101‑1 (MQ=255)
                                                          cgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              <  1:3879924/96‑1 (MQ=2)
                                                           cGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           <  1:406260/101‑1 (MQ=255)
                                                            tcAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  2:2010199/100‑1 (MQ=2)
                                                            ctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            <  2:1246216/98‑1 (MQ=2)
                                                             tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           <  1:2262273/99‑1 (MQ=2)
                                                             tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           >  1:692998/3‑101 (MQ=2)
                                                             cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                         <  2:1943643/101‑1 (MQ=255)
                                                             cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                         >  1:1203010/1‑101 (MQ=255)
                                                              ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                          <  1:3510966/100‑1 (MQ=2)
                                                              aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTg                                        >  1:1396361/1‑101 (MQ=255)
                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                          >  1:1665150/1‑100 (MQ=2)
                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                          <  2:3055567/101‑2 (MQ=2)
                                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                       >  1:3116456/1‑101 (MQ=255)
                                                               gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCt                                         <  1:1866704/101‑1 (MQ=2)
                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACCta                                        >  2:1509462/1‑100 (MQ=2)
                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg                                      >  2:139541/1‑101 (MQ=255)
                                                                gcGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCta                                        <  1:583479/100‑2 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.ATGACCAGTT..CCGCAATCGG..CCGGGCTCGATCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                       >  1:762208/1‑99 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                       <  2:2648117/101‑3 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                       <  1:523838/101‑3 (MQ=2)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACTNGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                       <  1:2007056/101‑5 (MQ=2)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                       <  1:143861/101‑5 (MQ=2)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccc                                       <  1:1122108/101‑5 (MQ=2)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                      <  2:642519/101‑6 (MQ=2)
                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGt                                  >  2:1103746/1‑101 (MQ=255)
                                                                     ccccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTg                                 <  1:2865686/101‑1 (MQ=255)
                                                                      cccccGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcg                                   >  1:608148/1‑93 (MQ=2)
                                                                        cccGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGTGCGTGGATCGAAAcacccggcgcc                                 <  1:4415426/101‑11 (MQ=2)
                                                                        cccGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcgcc                                 <  2:396788/101‑11 (MQ=2)
                                                                          cgcgGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTg                            >  2:2556226/1‑101 (MQ=255)
                                                                           gcgGGGGCGTGGATCGAAACT.GGTATG.GATCCG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGc                           <  2:4089459/101‑1 (MQ=255)
                                                                            cgGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGcc                          <  1:987025/101‑1 (MQ=255)
                                                                            cgGGGGCGTGGATCGAAACT.GGTATG.GATATG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGcc                          <  1:3221556/101‑1 (MQ=255)
                                                                             gggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                         >  2:4292067/1‑101 (MQ=255)
                                                                             gggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                         >  2:445663/1‑101 (MQ=255)
                                                                             gggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCa                         >  2:116345/1‑101 (MQ=255)
                                                                              ggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                        <  2:3788840/101‑1 (MQ=255)
                                                                              ggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAg                        >  2:3700339/1‑101 (MQ=255)
                                                                                   gTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATa                   >  2:2711495/1‑101 (MQ=255)
                                                                                    tGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg                  <  1:2461099/101‑1 (MQ=255)
                                                                                    tGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg                  >  2:3196633/1‑101 (MQ=255)
                                                                                    tGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAg                  <  2:3439032/101‑1 (MQ=255)
                                                                                     ggATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCT.CCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGAc                >  2:3760347/1‑101 (MQ=255)
                                                                                       aTCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg               <  1:229321/101‑1 (MQ=255)
                                                                                       aTCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg               <  2:1914804/101‑1 (MQ=255)
                                                                                       aTCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACg               >  1:4153850/1‑101 (MQ=255)
                                                                                        tCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGc              >  1:1702922/1‑101 (MQ=255)
                                                                                        tCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGc              <  2:4067758/101‑1 (MQ=255)
                                                                                          gAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCg            >  1:1273450/1‑101 (MQ=255)
                                                                                             aCT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTgg         >  2:260881/1‑101 (MQ=255)
                                                                                              cT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa        <  1:1146380/101‑1 (MQ=255)
                                                                                              cT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGa        <  2:145072/101‑1 (MQ=255)
                                                                                               t.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGAc       <  1:3097060/101‑1 (MQ=255)
                                                                                                 ggTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACg      <  1:1538061/101‑1 (MQ=255)
                                                                                                 ggTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACg      >  1:1311420/1‑101 (MQ=255)
                                                                                                    aTG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCg   >  2:1754223/1‑101 (MQ=255)
                                                                                                     |                                                                                                           
GCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGCATGTAGTCGGCGGGCAGTTCGAGCCGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGC  >  NC_014034/1395636‑1395836

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG